The effect of vitamin E, sodium selenite and "Santoquin" on the thiobarbituric acid values of pig liver homogenates

1969 ◽  
Vol 84 (5) ◽  
pp. 111-113 ◽  
Author(s):  
J. Connolly ◽  
J. O'Brien
Keyword(s):  
Vitamin E ◽  
Sodium Selenite ◽  
Thiobarbituric Acid ◽  
Pig Liver ◽  
Liver Homogenates

scholarly journals Selenium and α-tocopherol content in eggs produced by hens that were fed diets supplemented with selenomethionine, sodium selenite and vitamin E

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 388-397 ◽  
Author(s):  
M. Skřivan ◽  
I. Bubancová ◽  
M. Marounek ◽  
G. Dlouhá
Keyword(s):  
Vitamin E ◽  
Sodium Selenite ◽  
Thiobarbituric Acid ◽  
Basal Diet ◽  
Tocopherol Content ◽  
Alpha Tocopherol ◽  
Dietary Vitamin ◽  
Tocopherol Concentration ◽  
The Third ◽  
Egg Yolks

The effect of supplementing dietary selenium (Se) and vitamin E was investigated in 330 24-week-old laying hens. The hens were fed a basal diet containing Se and α-tocopherol at 0.11 and 26 mg/kg, respectively, or a diet supplemented with Se at 0.3 mg/kg and vitamin E between 0 and 625 mg/kg. Se was supplied as Se-methionine or sodium selenite. The eggs were collected for analysis during the third, seventh and eleventh weeks of the experiment. Supplementation of either form of Se significantly increased the Se concentration in egg yolks and whites, with a more pronounced effect caused by Se-methionine. The egg yolk α-tocopherol concentration paralleled the dietary α-tocopherol concentration. At a high dietary α-tocopherol concentration (632 mg/kg), the retinol content in egg yolks from hens fed Se-methionine increased significantly. Supplementation of Se-methionine significantly increased the α-tocopherol content in the eggs in the third and seventh weeks of the experiment. A moderate decrease in yolk cholesterol was observed in hens fed Se-methionine and α-tocopherol at 119 mg/kg. The concentration of products from lipid peroxidation (thiobarbituric acid-reactive substances, TBARS) in egg yolks increased marginally during the refrigerated storage of the eggs for 2 weeks. The effect of dietary vitamin E on TBARS formation was generally small, although a more significant effect was observed at the highest dose tested.

Die Bedeutung der Homogenisationsart und -intensität für die Größe und Konstanz der Sauerstoffaufnahme von Meerschweinchen-Leberhomogenat / The Influence of Mode and Intensity of Homogenization on the Absolute Value and Stability of Oxygen Consumption of Guinea Pig Liver Homogenates

1977 ◽  
Vol 32 (11-12) ◽  
pp. 908-912 ◽  
Author(s):  
H. J. Schmidt ◽  
U. Schaum ◽  
J. P. Pichotka
Keyword(s):  
Oxygen Uptake ◽  
Guinea Pig ◽  
Measuring Time ◽  
Pig Liver ◽  
Absolute Value ◽  
Modified Method ◽  
Simultaneous Measurements ◽  
Liver Homogenates ◽  
The Absolute ◽  
Guinea Pig Liver

Abstract The influence of five different methods of homogenisation (1. The method according to Potter and Elvehjem, 2. A modification of this method called Potter S, 3. The method of Dounce, 4. Homogenisation by hypersonic waves and 5. Coarce-grained homogenisation with the “Mikro-fleischwolf”) on the absolute value and stability of oxygen uptake of guinea pig liver homogenates has been investigated in simultaneous measurements. All homogenates showed a characteristic fall of oxygen uptake during measuring time (3 hours). The modified method according to Potter and Elvehjem called Potter S showed reproducible results without any influence by homogenisation intensity.

scholarly journals COMPARISONS OF THE OXIDATION OF C19-HYDROXYSTEROIDS BY GUINEA PIG LIVER HOMOGENATES

1957 ◽  
Vol 224 (2) ◽  
pp. 811-818
Author(s):  
Charles D. Kochakian ◽  
Betty R. Carroll ◽  
Barbara Uhri
Keyword(s):  
Guinea Pig ◽  
Pig Liver ◽  
Liver Homogenates ◽  
Guinea Pig Liver

Effect of Sodium Selenite and Vitamin E on the Renal Cortex in Rats: An Ultrastructure Study

2014 ◽  
Vol 46 (3) ◽  
pp. 170-177 ◽  
Author(s):  
Adel M. Hussein ◽  
Hamid A. Saleh ◽  
Mustafa H.N.
Keyword(s):  
Vitamin E ◽  
Sodium Selenite ◽  
Renal Cortex ◽  
Ultrastructure Study

scholarly journals Oxidative stability of tilapia feeds containing Saccharomyces cerevisiae and Spirulina platensis

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Juliana Sedlacek-Bassani ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vitamin E ◽  
Spirulina Platensis ◽  
Nile Tilapia ◽  
Room Temperature ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Rancidity ◽  
Plastic Bags

ABSTRACT: The aim of this study was to evaluate the effect of the inclusion of microbial biomass on the oxidative rancidity of tilapia rations stored for 12 months. Treatments included a control diet and diets supplemented with either 0.01% vitamin E, 0.25 and 0.5% of Saccharomyces cerevisiae and 0.25 and 0.5% of Spirulina platensis. Experimental diets were stored in the dark inside plastic bags at room temperature (25 °C) for 12 months. The oxidative rancidity was measured as thiobarbituric acid reactive substances (TBARS). It was concluded that the inclusions of Spirulina platensis at 0.25% (1.734 ± 0.206) and 0.5% (1.629 ± 0.181) and Saccharomyces cerevisiae at 0.5% (1.459 ± 0.305) minimized the oxidative rancidity in comparation to control diet (2.843 ± 0.109) of Nile tilapia until 12 months of storage.

Glutathione peroxidase, selenium, and prostaglandin synthesis in platelets

1981 ◽  
Vol 240 (5) ◽  
pp. H800-H803 ◽  
Author(s):  
M. G. Doni ◽  
G. L. Avventi ◽  
L. Bonadiman ◽  
G. Bonaccorso
Keyword(s):  
Glutathione Peroxidase ◽  
Dietary Intake ◽  
G Protein ◽  
Sodium Selenite ◽  
Thiobarbituric Acid ◽  
Prostaglandin Synthesis ◽  
Breakdown Product ◽  
Standard Diet ◽  
Organic Hydroperoxides ◽  
Selenium Treatment

Glutathione peroxidase (GSH-Px) contains 4 selenium atoms/molecule; its activity is increased by selenium dietary intake. The enzyme destroys H2O2 and organic hydroperoxides, contributing to the integrity of biological membranes. GSH-Px activity increased (+100%) in washed platelets of rats administered selenium (0.3 ppm given as sodium selenite) for 60 days from 10.44 +/- 1.10 U/g protein (control rats fed a standard diet) to 20.50 +/- 1.21 U/g protein (mean +/- SE; P less than 0.001). GSH-Px in washed erythrocytes was also stimulated (+70%) after 80 days of selenium dietary intake from 11.60 +/0 0.82 U/g Hb to 19.74 +/- 0.94 U/g Hb (P less than 0.001). Malondialdehyde (MDA), the typical breakdown product of peroxidized lipid and a suitable indicator of platelet prostaglandin production, increased from 0.343 +/- 0.035 nM/3 X 10(8) platelets (control) to 0.478 +/- 0.052 nM/3 X 10(8) platelets after 30 days of selenium treatment (P less than 0.05) and to 0.527 +/- 0.051 nM/3 X 10(8) platelets after 80 days (P less than 0.01). MDA was measured by the thiobarbituric acid method after stimulation with 25 X 10(-4) M arachidonic acid. It is concluded that platelets are very rich in GSH-Px, i.e., activity is greatly increased by oral administration of selenium and that the synthesis of prostaglandins is stimulated too.

scholarly journals Markers of oxidative and antioxidative activity in female dogs with mammary gland tumour with and without additional vitamin E supplementation

2012 ◽  
Vol 81 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Renata Stavinohová ◽  
Jana Lorenzová ◽  
Ivana Papežíková ◽  
Ivana Borkovcová ◽  
Jakub Pfeifr ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Vitamin E ◽  
Antioxidative Activity ◽  
Thiobarbituric Acid ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Antioxidative Status ◽  
Group 2 ◽  
Gland Tumour

The present study determined markers of oxidative and antioxidative activity in dog females affected with mammary gland tumour compared to healthy ones. The effect of additional vitamin E supplementation on oxidative and antioxidative status was evaluated as well. The study included 29 female dogs divided into 4 groups (groups 1 and 2 included females with a mammary gland tumour; groups 3 and 4 included healthy female dogs). Additional vitamin supplement containing α-tocopherol was given to the females of groups 1 and 4. Dogs from groups 1 and 2 were anaesthetized before surgery (ovariohysterectomy and mastectomy); anaesthesia was used also in group 3, but without performeing surgery. The content of vitamin E (free α-tocopherol), marker of antioxidative status, was measured in blood serum by liquid chromatography. The concentration of thiobarbituric acid reactive substances, marker of oxidative status, in serum and concentrations of protein and non-protein thiol groups, markers of oxidative and antioxidative status, in blood serum and in red blood cells were measured colorimetrically. In females with a mammary gland tumour from group 2, concentration of thiobarbituric acid reactive substances was significantly higher than 14 days after surgery and compared to healthy ones as well. In females with a mammary gland tumour from group 2, concentration of protein thiol groups in serum was significantly lower and concentration of non-protein thiol groups in serum was significantly higher than in healthy controls. The values of protein thiols in erythrocytes in females with mammary gland tumour from group 1 were significantly higher before supplementation with vitamin E. The present study revealed that females with a mammary gland tumour were more burdened with oxidative stress compared to healthy dogs. The removal of the mammary gland tumour led to improvement of oxidative and antioxidative status. This is the first report focusing on the effect of additional α-tocopherol supplementation on reducing oxidative stress by increasing antioxidative activity in females affected with mammary gland tumour; however, we did not prove it.

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