scholarly journals Markers of oxidative and antioxidative activity in female dogs with mammary gland tumour with and without additional vitamin E supplementation

2012 ◽  
Vol 81 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Renata Stavinohová ◽  
Jana Lorenzová ◽  
Ivana Papežíková ◽  
Ivana Borkovcová ◽  
Jakub Pfeifr ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Vitamin E ◽  
Antioxidative Activity ◽  
Thiobarbituric Acid ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Antioxidative Status ◽  
Group 2 ◽  
Gland Tumour

The present study determined markers of oxidative and antioxidative activity in dog females affected with mammary gland tumour compared to healthy ones. The effect of additional vitamin E supplementation on oxidative and antioxidative status was evaluated as well. The study included 29 female dogs divided into 4 groups (groups 1 and 2 included females with a mammary gland tumour; groups 3 and 4 included healthy female dogs). Additional vitamin supplement containing α-tocopherol was given to the females of groups 1 and 4. Dogs from groups 1 and 2 were anaesthetized before surgery (ovariohysterectomy and mastectomy); anaesthesia was used also in group 3, but without performeing surgery. The content of vitamin E (free α-tocopherol), marker of antioxidative status, was measured in blood serum by liquid chromatography. The concentration of thiobarbituric acid reactive substances, marker of oxidative status, in serum and concentrations of protein and non-protein thiol groups, markers of oxidative and antioxidative status, in blood serum and in red blood cells were measured colorimetrically. In females with a mammary gland tumour from group 2, concentration of thiobarbituric acid reactive substances was significantly higher than 14 days after surgery and compared to healthy ones as well. In females with a mammary gland tumour from group 2, concentration of protein thiol groups in serum was significantly lower and concentration of non-protein thiol groups in serum was significantly higher than in healthy controls. The values of protein thiols in erythrocytes in females with mammary gland tumour from group 1 were significantly higher before supplementation with vitamin E. The present study revealed that females with a mammary gland tumour were more burdened with oxidative stress compared to healthy dogs. The removal of the mammary gland tumour led to improvement of oxidative and antioxidative status. This is the first report focusing on the effect of additional α-tocopherol supplementation on reducing oxidative stress by increasing antioxidative activity in females affected with mammary gland tumour; however, we did not prove it.

scholarly journals Oxidation-antioxidant balance in the colon mucosa of rats at different times points after ceftriaxone administration

2017 ◽  
Vol 22 (1) ◽  
pp. 11-15
Author(s):  
Y. Holota ◽  
A. Bazan ◽  
G. Tolstanova
Keyword(s):  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Colon Mucosa ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Sod Activity ◽  
Control Value ◽  
Active Substances

Сeftriaxone administration for 14 days (300 mg/kg, i.m.) increased level of thiobarbituric acid reactive substances and decreased the activity of superoxide dismutase and catalase antioxidant enzymes in the colon mucosa of rats immediately after antibiotics injection. On the 29th day of the experiment (in 14 days after ceftriaxone withdrawal) the level of TBA-active substances still 2.5-fold (P <0.05) exceeded the control value and SOD activity remained below control values to the 72nd day of the experiment. These were accompanied by decreased level of protein thiol groups in 15 and 29 days 1.9-fold (P <0.05) and 1.4-fold (P = 0.08), respectively. Thus antibiotics can lead to long-term oxidative disturbance in the colon mucosa of rats.

scholarly journals Oxidative stability of tilapia feeds containing Saccharomyces cerevisiae and Spirulina platensis

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Juliana Sedlacek-Bassani ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vitamin E ◽  
Spirulina Platensis ◽  
Nile Tilapia ◽  
Room Temperature ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Rancidity ◽  
Plastic Bags

ABSTRACT: The aim of this study was to evaluate the effect of the inclusion of microbial biomass on the oxidative rancidity of tilapia rations stored for 12 months. Treatments included a control diet and diets supplemented with either 0.01% vitamin E, 0.25 and 0.5% of Saccharomyces cerevisiae and 0.25 and 0.5% of Spirulina platensis. Experimental diets were stored in the dark inside plastic bags at room temperature (25 °C) for 12 months. The oxidative rancidity was measured as thiobarbituric acid reactive substances (TBARS). It was concluded that the inclusions of Spirulina platensis at 0.25% (1.734 ± 0.206) and 0.5% (1.629 ± 0.181) and Saccharomyces cerevisiae at 0.5% (1.459 ± 0.305) minimized the oxidative rancidity in comparation to control diet (2.843 ± 0.109) of Nile tilapia until 12 months of storage.

scholarly journals Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production

1998 ◽  
Vol 7 (5) ◽  
pp. 313-317 ◽  
Author(s):  
V. Bocci ◽  
G. Valacchi ◽  
F. Corradeschi ◽  
G. Fanetti
Keyword(s):  
Antioxidant Status ◽  
Cytokine Production ◽  
Biological Effects ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Cytokine Induction ◽  
Wide Range ◽  
Total Antioxidant

Ozone (O3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While the disinfectant activity of O3is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O3concentrations between 40 and 80μ g/ml per gram of blood (˜0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to fivefold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O3decomposition, acts as a messenger for the cytokine induction.

scholarly journals Dietary lipid sources and vitamin E affect fatty acid composition or lipid stability of breast meat from Muscovy duck

2010 ◽  
Vol 90 (3) ◽  
pp. 371-378 ◽  
Author(s):  
A. Schiavone ◽  
M. Marzoni ◽  
A. Castillo ◽  
J. Nery ◽  
I. Romboli
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Vitamin E ◽  
Fish Oil ◽  
Acid Composition ◽  
Thiobarbituric Acid ◽  
Muscovy Duck ◽  
Thiobarbituric Acid Reactive Substances ◽  
Breast Meat ◽  
Lipid Stability

A trial was conducted in order to assess the fatty acid composition and lipid stability of breast meat from Muscovy ducks (Cairina moschata domestica L.) fed a basal diet supplemented with 20 g kg-1 of soybean oil or fish oil and 30 or 230 mg kg-1 α-tocopheryl acetate (α-TA). A total of 120 one-day-old female muscovy ducklings were distributed over 12 pens (10 birds/pen). Growth performance traits were measured through the study. Each dietary treatment was randomly administered to three replicates when birds were 43 d until the slaughtering age of 66 d. Dietary treatments did not induce differences in growth performances and slaughter traits. Similarly, proximate composition and pH of breast muscle were not influenced by the diets. Significant increases in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents in breast meat of ducks fed fish-oil-supplemented diets was observed. Lipid oxidation, expressed as thiobarbituric acid reactive substances (TBARS), of breast meat from birds fed 230 mg kg-1 α-TA-supplemented diets was significantly lower, compared with those fed diets supplemented with 30 mg kg-1 α-TA, both 1 and 7 d after slaughter.Key words: Duck, meat, fatty acid, vitamin E, thiobarbituric acid reactive substances

Thiobarbituric acid reactive substances and vitamin E in healthy women and patients with pregnancy-induced hypertension

2001 ◽  
Vol 1 (5) ◽  
pp. 259-266
Author(s):  
N[eacute]stor J. Aparicio ◽  
Mirta Joao ◽  
Pablo Fernandez ◽  
Marta Cortelezzi ◽  
Viviana Caram[eacute]s ◽  
...  
Keyword(s):  
Vitamin E ◽  
Thiobarbituric Acid ◽  
Pregnancy Induced Hypertension ◽  
Thiobarbituric Acid Reactive Substances ◽  
Healthy Women ◽  
Induced Hypertension

Diaphragmatic function after resistive breathing in vitamin E-deficient rats

1993 ◽  
Vol 74 (1) ◽  
pp. 267-271 ◽  
Author(s):  
A. Anzueto ◽  
F. H. Andrade ◽  
L. C. Maxwell ◽  
S. M. Levine ◽  
R. A. Lawrence ◽  
...  
Keyword(s):  
Vitamin E ◽  
Contractile Function ◽  
Thiobarbituric Acid ◽  
Force Frequency ◽  
Sprague Dawley ◽  
Thiobarbituric Acid Reactive Substances ◽  
Vitamin E Deficiency ◽  
Deficient Diet ◽  
Resistive Breathing

The effects of vitamin E deficiency on diaphragm function were studied at rest and after resistive breathing (RB) in Sprague-Dawley rats (wt 300–400 g). The animals were pair fed a vitamin E-deficient diet (E-def) or a matched vitamin E-sufficient diet (E-suf). Each diet group was then further subdivided into a group that breathed unimpeded (control) and a second group that breathed through an inspiratory resistor until the animals were unable to sustain 70% of their maximum airway pressure. Diaphragm samples were obtained for analysis of thiobarbituric acid-reactive substances, glutathione (GSH) concentrations, and glutathione disulfide (GSSG) concentrations. In vitro isometric contractile studies were also performed and included twitch (Pt) and maximum tetanic (Po) tensions, force-frequency curves, fatigue index, and recovery index. Pt was significantly reduced in the E-suf RB group as well as both of the E-def groups. Po was also significantly reduced in both E-def groups. The E-def rats subjected to RB showed a significant decrease in tension at both high and low frequencies compared with the E-suf rats. Concentrations of diaphragm thiobarbituric acid-reactive substances were significantly increased in both E-def groups. RB in both E-suf and E-def rats resulted in increases in diaphragm concentrations of GSSG and decreases in the GSH/GSSG ratios. We conclude that reduction of contractile function, lipid peroxidation, and activation of the GSH redox cycle occur with RB and that these effects are significantly increased in the presence of vitamin E deficiency.

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