Photosynthesis and CO2 evolution by leaf discs: gas exchange, extraction, and ion-exchange fractionation of 14C-labeled photosynthetic products

1971 ◽  
Vol 49 (7) ◽  
pp. 1225-1234 ◽  
Author(s):  
C. A. Atkins ◽  
D. T. Canvin

A leaf chamber is described which allows the measurement of the gas exchange of leaf discs in an open gas analysis system. Pretreatment of the leaf discs with nitrogen and inclusion of moist filter paper in the leaf chamber resulted in rates of photosynthesis that were constant for 40 min and equal to 60 to 70% of the rate of photosynthesis measured in intact leaves. Analysis of the products of 14CO2 fixation from whole leaves and leaf discs and a comparison of the gas exchange under various conditions established that the leaf discs accurately reflected the metabolism of the attached leaves.

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 540a-540
Author(s):  
K.J. Prevete ◽  
R.T. Fernandez

Three species of herbaceous perennials were tested on their ability to withstand and recover from drought stress periods of 2, 4, and 6 days. Eupatorium rugosum and Boltonia asteroides `Snowbank' were chosen because of their reported drought intolerance, while Rudbeckia triloba was chosen based on its reported drought tolerance. Drought stress began on 19 Sept. 1997. Plants were transplanted into the field the day following the end of each stress period. The effects of drought on transpiration rate, stomatal conductance, and net photosynthetic rate were measured during the stress and throughout recovery using an infrared gas analysis system. Leaf gas exchange measurements were taken through recovery until there were no differences between the stressed plants and the control plants. Transpiration, stomatal conductance, and photosynthesis of Rudbeckia and Boltonia were not affected until 4 days after the start of stress. Transpiration of Eupatorium decreased after 3 days of stress. After rewatering, leaf gas exchange of Boltonia and Rudbeckia returned to non-stressed levels quicker than Eupatorium. Growth measurements were taken every other day during stress, and then weekly following transplanting. Measurements were taken until a killing frost that occurred on 3 Nov. There were no differences in the growth between the stressed and non-stressed plants in any of the species. Plants will be monitored throughout the winter, spring, and summer to determine the effects of drought on overwintering capability and regrowth.


1994 ◽  
Vol 40 (1) ◽  
pp. 124-129 ◽  
Author(s):  
R J Wong ◽  
J J Mahoney ◽  
J A Harvey ◽  
A L Van Kessel

Abstract We evaluated a new portable instrument, the PPG StatPal II pH and Blood Gas Analysis System, designed for "point-of-care" measurements of blood gases and pH. Inaccuracy (% of target value) and imprecision (CV%) were assessed by blood tonometry and comparison with a Corning 178. Within-day results for PCO2 inaccuracy and imprecision ranged from 98.2% to 102.9% and 3.3% to 3.9%, respectively; for PO2, these were 95.5% to 102.3% and 2.3% to 3.0%, respectively. Between-day results for PCO2 inaccuracy and imprecision ranged from 99.2% to 99.3% and from 2.9% to 3.2%, respectively; for PO2, the ranges were 96.2% to 98.2% and 2.6% to 3.0%, respectively. Two PCO2 outliers (in 645 samples = 0.3%) were observed. In general, tonometry recovery, measurement stability, and pH bias results for the StatPal II and Corning 178 were comparable. We conclude that the StatPal II performs within acceptable ranges of inaccuracy and imprecision.


2011 ◽  
Vol 9 (6) ◽  
pp. 060101-60104 ◽  
Author(s):  
孙友文 Youwen Sun ◽  
刘文清 Wenqing Liu ◽  
汪世美 Shimei Wang ◽  
黄书华 Shuhua Huang ◽  
于晓曼 Xiaoman Yu
Keyword(s):  

2021 ◽  
Author(s):  
Tiago DG Nunes ◽  
Magdalena W Slawinska ◽  
Heike Lindner ◽  
Michael T Raissig

Stomata are cellular pores on the leaf epidermis that allow plants to regulate carbon assimilation and water loss. Stomata integrate environmental signals to regulate pore apertures and optimize gas exchange to fluctuating conditions. Here, we quantified intraspecific plasticity of stomatal gas exchange and anatomy in response to seasonal variation in Brachypodium distachyon. Over the course of two years we (i) used infrared gas analysis to assess light response kinetics of 120 Bd21-3 wild-type individuals in an environmentally fluctuating greenhouse and (ii) microscopically determined the seasonal variability of stomatal anatomy in a subset of these plants. We observed systemic environmental effects on gas exchange measurements and remarkable intraspecific plasticity of stomatal anatomical traits. To reliably link anatomical variation to gas exchange, we adjusted anatomical gsmax calculations for grass stomatal morphology. We propose that systemic effects and variability in stomatal anatomy should be accounted for in long-term gas exchange studies.


1971 ◽  
Vol 24 (4) ◽  
pp. 1077 ◽  
Author(s):  
MM Ludlow ◽  
GL Wilson

Grass and legume plants were grown under near-optimum conditions in controlled-environment cabinets. Changes in net photosynthetic rate, dark respiration rate, and carbon dioxide transfer resistances during leaf ontogeny, and variability between leaves on grass tillers and legume runners were studied under controlled conditions in an open gas analysis system.


2021 ◽  
Vol 8 (12) ◽  
pp. 164
Author(s):  
Eleftherios Karatzanos ◽  
Panagiotis Ferentinos ◽  
Georgios Mitsiou ◽  
Stavros Dimopoulos ◽  
Argyrios Ntalianis ◽  
...  

The purpose of this study was to compare the acute cardiorespiratory responses and time spent above different %VO2peak intensities between three “iso-work” protocols: (a) a high intensity interval training protocol (HIIT), (b) a higher intensity continuous protocol (CON70) and (c) a lower intensity continuous protocol (CON50) in patients with chronic heart failure (CHF). Ten male CHF patients (aged 55.1 ± 16.2 years) performed in separate days a single session of a HIIT protocol consisted of 4 sets × 4 min cycling at 80% VO2peak with 3 min of recovery at 50% VO2peak, a CON70 protocol corresponding to 70% VO2peak and a CON50 protocol corresponding to 50% VO2peak. Cardiopulmonary data were collected by an online gas analysis system. The HIIT and CON70 elicited higher cardiorespiratory responses compared to CON50 with no differences between them (p > 0.05). In HIIT and CON70, patients exercised longer at >80% and >90% VO2peak. The completion rate was 100% for the three protocols. Not any adverse events were observed in either protocol. Both HIIT and CON70 elicited a stronger physiological stimulus and required shorter time than CON50. Both HIIT and CON70 also induced comparable hemodynamic responses and ventilatory demand.


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