Addition of Clinoptilolite In The Diet Reduces Uterine PMN Leukocytes And Open Days In Multiparous Lactating Dairy Cows Managed In A Mountain Tropical Pasture-Based System

This study was aimed to assess the effect of adding clinoptilolite in the diet on uterine health and reproductive performance in multiparous lactating dairy cows managed in a tropical pasture-based system above 2500 meters of altitude. Seventy-seven multiparous Holstein crossbred cows were allocated randomly into two groups: clinoptilolite supplemented cows (CLG, n = 42) and non-supplemented cows as control (CG, n = 35). Cows from CLG were supplemented with clinoptilolite from 30 days (50 g/cow/day) before to 60 days after calving (200 g/cow/day). Data were analyzed by general linear model, least squares means and chi-square test of SAS. In CLG cows, percentages of uterine PMN leukocytes (P<0.0001) and proportion of subclinical endometritis (P=0.0187) were lower than in CG. The interval calving to first corpus luteum was shorter (P=0.0759) in CLG than CG, and calving to first service interval was similar between treatments. Cows from CLG became pregnant 35 days earlier than CG cows (P=0.0224). In conclusion, daily addition of clinoptilolite in the diet decreased the proportion of cows with subclinical endometritis and shortened the interval from calving to conception in lactating dairy cows.

Influence of energy supplementation on associative effects in Nellore bulls on a tropical pasture during the rainy season

The objective of this study was to evaluate the influence of energy supplementation on associative effects between forage and concentrate supplements and on the performance of beef cattle on a tropical pasture during the rainy season. Twenty-eight Nellore bulls averaging 19 months of age and 325.2 ± 4.1 kg were allotted to a completely randomized design and distributed into four paddocks of two hectares each with a tropical pasture for 84 d. The treatments consisted of bulls on Urochloa brizantha cv. Xaraés pasture without concentrate supplementation or bulls on Urochloa brizantha cv. Xaraés pasture receiving concentrate supplementation at 15 (S15), 30 (S30) or 45% (S45) of the total digestible nutrients (TDN) requirement All bulls had ad libitum access to mineral supplementation. The amount and chemical composition of the supplement were adjusted every 28 d. Forage and supplement intakes were evaluated in all animals using fecal markers. Crude protein: digestible organic matter ratio decreased with supplementation; consequently, the imbalance between dietary protein and energy increased. Forage intake decreased with increasing supplementation. The substitution rates of pasture for supplementary feed were 0.14, 0.33, and 0.46 for bulls from S15, S30, and S45 treatments, respectively. However, the apparent DM digestibility increased linearly with supplementation level. Supplementation improved the assimilation of ammonia-N in the rumen. Energy supplementation for Nellore bulls on tropical pastures during the rainy season provides excess energy relative to protein, resulting in a substitution effect of supplement intake on forage intake. Moreover, energy supplementation does not improve body weight gain. Protein sources should be added to supplements for balancing protein and energy levels, thereby reducing a likely substitution effect.

Influence of tropical upright pasture structural and chemical characteristics on lamb grazing time

Tropical pasture canopy characteristics can alter lamb ingestive behavior. Our study evaluated the ingestive behavior of young lambs in different tropical pastures to identify which variables interfere in their grazing activity. Two years of study were carried out with 54 weaned lambs distributed in three different pasture canopies: 1) monoculture of an upright grass, guinea grass (Panicum maximum; GG); 2) monoculture of a shrubby legume pigeon pea (Cajanus cajan; PP) and 3) contiguous paddock with half GG and half PP (GP). The experiment was set out in a randomized complete block design (3 blocks). Lamb ingestive behavior was observed from sunrise to sunset with records every 5 minutes. To identify the main variables that affected lamb grazing activity, a multivariate analysis of the Decision Tree was performed. Our results showed that there was no difference in the ingestive behavior parameters of young lambs in different canopies (P > 0.05). There was interaction among the canopies and the experimental periods for the variables idleness time and biting rate (P ≤ 0.05). Lambs in all canopies showed more idleness time in the first evaluation period. Lambs in canopies containing grass (GG and GP) exhibited greater bites per minute throughout the experimental period. Lamb grazing time increased 40% as experimental period progressed and plants matured. The Decision Tree identified leaf:stem ratio as the variable that most influenced lamb grazing time in GG and GP canopies while in the PP, grazing time was directly related to canopy height. The behavior of young lambs on tropical pasture is variable as there is a change in the behavioral response to canopy characteristics over time. In addition, the grazing time of these animals can be estimated by means of variables related to canopy structural characteristics (leaf:stem ratio and height) together with chemical variables.