Changes in the amount and composition of fractions from extracellular polysaccharide during the culture cycle of cotyledon cell suspension culture of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (3-4) ◽  
pp. 198-201 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Suspension Culture ◽  
Cell Suspension Culture ◽  
Cold Water ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
Neutral Sugars ◽  
Culture Cycle

Three different polysaccharides (cold-water-soluble pectin, EDTA-soluble pectin, and neutral polysaccharides) were separated from extracellular polysaccharide produced by bush-bean cotyledon suspension cultures. The different polysaccharides were associated with different phases of the culture cycle. The neutral sugars present in the pectin fractions showed considerable fluctuation during the culture cycle as compared with the rather small changes in the levels of the sugars present in the neutral polysaccharides.

Comparison of growth and extracellular polysaccharide of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender) grown in coconut-milk medium and synthetic medium

1975 ◽  
Vol 53 (15) ◽  
pp. 1542-1548 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Synthetic Medium ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Coconut Milk ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
Neutral Sugars ◽  
Culture Cycle

Growth and extracellular polysaccharide (EP) production by cotyledon suspension cultures of bush bean were compared in coconut-milk (CM) medium and synthetic medium. EP was produced at all stages of the culture cycle investigated. The same neutral sugars were present in the EP recovered from both media and the composition showed some changes during the culture cycle. Factors in coconut milk had relatively little effect on the neutral sugar composition of the EP.

Extracellular polysaccharide from cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1972 ◽  
Vol 50 (10) ◽  
pp. 2031-2037 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Higher Plants ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Cell Number ◽  
Suspension Cultures ◽  
Logarithmic Phase ◽  
Bush Bean ◽  
Culture Cycle ◽  
High Yields

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.

Isoenzymes in cell cultures of bush bean (Phaseolus vulgaris cv. Contender): isoenzymatic changes during the callus culture cycle and differences between stock cultures

1974 ◽  
Vol 52 (12) ◽  
pp. 2621-2629 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Polyphenol Oxidase ◽  
Callus Culture ◽  
Cell Cultures ◽  
Callus Cultures ◽  
Bush Bean ◽  
Leucine Amino Peptidase ◽  
Culture Cycle ◽  
Enzyme Patterns ◽  
Isoenzyme Patterns

Electrophoretic analyses of isoenzyme patterns were performed with extracts of root, hypocotyl, and cotyledon callus cultures derived from a single seedling. The enzymes studied included peroxidase, polyphenol oxidase, catalase, malate and glutamate dehydrogenases, esterase, and leucine amino peptidase. Enzyme patterns changed during the culture cycle and several isoenzymes appeared only at certain times. The isoenzymatic patterns of the three cultures were very similar but persistent differences between them were observed.

Effect of either 2,4-dichlorophenoxyacetic acid or kinetin on production and composition of various fractions from extracellular polysaccharides produced by cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (15) ◽  
pp. 1816-1822 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Extracellular Polysaccharides ◽  
Dichlorophenoxyacetic Acid ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
2,4 Dichlorophenoxyacetic Acid

Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.

scholarly journals Transient Gene Expression in Protoplasts of Phaseolus vulgaris Isolated from a Cell Suspension Culture

1991 ◽  
Vol 95 (3) ◽  
pp. 968-972 ◽  
Author(s):  
Patricia Leon ◽  
Frederique Planckaert ◽  
Virginia Walbot
Keyword(s):  
Gene Expression ◽  
Phaseolus Vulgaris ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Transient Gene Expression ◽  
A Cell

Ag+-induced silymarin production in cell suspension culture of Silybum marianum L. Gaertn

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
SR Ashtiani ◽  
T Hasanloo ◽  
MR Bihamta
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Silybum Marianum

Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
CP Stefanache ◽  
N Imseng ◽  
B Meier ◽  
C Tanase ◽  
R Eibl-Schindler ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Arnica Montana ◽  
L Cell ◽  
Biotechnological Approach ◽  
Bioactive Secondary Metabolites

Grafting pH‐Responsive Copolymers to Cold Water‐Soluble Starch Using Nitroxide‐Mediated Polymerization

2021 ◽  
pp. 2100011
Author(s):  
Alexander T. Fritz ◽  
Jaime C. Cazotti ◽  
Omar Garcia‐Valdez ◽  
Niels M. B. Smeets ◽  
Marc A. Dubé ◽  
...  
Keyword(s):  
Cold Water ◽  
Soluble Starch ◽  
Water Soluble ◽  
Ph Responsive ◽  
Nitroxide Mediated Polymerization

Jam processing: Effect of pectin replacement by locust bean gum on its characteristics

2020 ◽  
pp. 1-12
Author(s):  
Aida Mekhoukhe ◽  
Nacer Mohellebi ◽  
Tayeb Mohellebi ◽  
Leila Deflaoui-Abdelfettah ◽  
Sonia Medouni-Adrar ◽  
...  
Keyword(s):  
Cold Water ◽  
Physicochemical Characteristics ◽  
Water Soluble ◽  
Sensory Profile ◽  
Locust Bean Gum ◽  
Locust Bean ◽  
Preference Map ◽  
The Right ◽  
High Level ◽  
The Impact

OBJECTIVE: the present work proposed to extract Locust Bean Gum (LBG) from Algerian carob fruits, evaluate physicochemical and rheological properties (solubility). It aimed also to develop different formulations of strawberry jams with a mixture of LBG and pectin in order to obtain a product with a high sensory acceptance. METHODS: the physicochemical characteristics of LBG were assessed. The impact of temperature on solubility was also studied. The physical and the sensory profile and acceptance of five Jams were evaluated. RESULTS: composition results revealed that LBG presented a high level of carbohydrate but low concentrations of fat and ash. The LBG was partially cold-water-soluble (∼62% at 25°C) and needed heating to reach a higher solubility value (∼89% at 80 °C). Overall, the sensorial acceptances decreased in jams J3 which was formulated with 100% pectin and commercial one (J5). The external preference map explained that most consumers were located to the right side of the map providing evidence that most samples appreciated were J4 and J2 (rate of 80–100%). CONCLUSION: In this investigation, the LBG was used successfully in the strawberry jam’s formulation.

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