Embryo and endosperm development in ovules of Papaver nudicaule after in vitro placental pollination

1981 ◽  
Vol 59 (9) ◽  
pp. 1738-1748 ◽  
Author(s):  
A. Randall Olson

Embryo development in Papaver nudicaule L. following in vitro placental pollination is of the Solanad type. Nuclear endosperm develops within 2–3 days and a filamentous proembryo is established. Endosperm cellularization is underway at the time the embryo proper begins to differentiate from the terminal and subterminal cells of the proembryo. After 6 days, the highly vacuolate endosperm is completely cellular; there is also a small globular embryo with no apparent endosperm degradation. Endosperm breakdown is initiated at the embryo–endosperm interface between 8 and 9 days postpollination. At day 10 of incubation, endosperm cytoplasm is dense and there is a large globular embryo. Substantial crenulation of the outer tangential wall of the protoderm occurs at this time. This phenomenon is presumably related to aspects of endosperm degradation. The primary tissue systems are discernable between 12 and 15 days when cotyledon initiation occurs. Seed development is complete within 27–28 days after placental pollination and the majority of the seeds germinate. This experimental approach provides a useful system for the study of a number of factors influencing seed ontogeny.

Author(s):  
Karunesh Makker ◽  
Prince Patel ◽  
Hrishikesh Roy ◽  
Sonali Borse

Stock market is a very volatile in-deterministic system with vast number of factors influencing the direction of trend on varying scales and multiple layers. Efficient Market Hypothesis (EMH) states that the market is unbeatable. This makes predicting the uptrend or downtrend a very challenging task. This research aims to combine multiple existing techniques into a much more robust prediction model which can handle various scenarios in which investment can be beneficial. Existing techniques like sentiment analysis or neural network techniques can be too narrow in their approach and can lead to erroneous outcomes for varying scenarios. By combing both techniques, this prediction model can provide more accurate and flexible recommendations. Embedding Technical indicators will guide the investor to minimize the risk and reap better returns.


1956 ◽  
Vol 15 (4) ◽  
pp. 1180-1187 ◽  
Author(s):  
C. N. Huhtanen ◽  
R.F. Elliott

2013 ◽  
Vol 39 (10) ◽  
pp. 1875-1886 ◽  
Author(s):  
Renate W. Boekhoven ◽  
Richard G.P. Lopata ◽  
Marc R. van Sambeek ◽  
Frans N. van de Vosse ◽  
Marcel C.M. Rutten

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 421-430 ◽  
Author(s):  
A Pellegrineschi ◽  
L M Noguera ◽  
B Skovmand ◽  
R M Brito ◽  
L Velazquez ◽  
...  

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.Key words: wheat transformation, 'Bobwhite', genotype effect.


Transfusion ◽  
1982 ◽  
Vol 22 (5) ◽  
pp. 355-358 ◽  
Author(s):  
JS Hunt ◽  
ML Beck ◽  
GE Tegtmeier ◽  
WL Bayer

Author(s):  
Babajide A Ojo ◽  
Kelli L VanDussen ◽  
Michael J Rosen

Abstract Physiologic, molecular, and genetic findings all point to impaired intestinal epithelial function as a key element in the multifactorial pathogenesis of ulcerative colitis (UC). The lack of epithelial-directed therapies is a conspicuous weakness of our UC therapeutic armamentarium. However, a critical barrier to new drug discovery is the lack of preclinical human models of UC. Patient tissue–derived colon epithelial organoids (colonoids) are primary epithelial stem cell–derived in vitro structures capable of self-organization and self-renewal that hold great promise as a human preclinical model for UC drug development. Several single and multi-tissue systems for colonoid culture have been developed, including 3-dimensional colonoids grown in a gelatinous extracellular matrix, 2-dimensional polarized monolayers, and colonoids on a chip that model luminal and blood flow and nutrient delivery. A small number of pioneering studies suggest that colonoids derived from UC patients retain some disease-related transcriptional and epigenetic changes, but they also raise questions regarding the persistence of inflammatory transcriptional programs in culture over time. Additional research is needed to fully characterize the extent to which and under what conditions colonoids accurately model disease-associated epithelial molecular and functional aberrations. With further advancement and standardization of colonoid culture methodology, colonoids will likely become an important tool for realizing precision medicine in UC.


Sign in / Sign up

Export Citation Format

Share Document