Protein bodies in umbelliferous seeds. II. Elemental composition

1982 ◽  
Vol 60 (8) ◽  
pp. 1392-1398 ◽  
Author(s):  
Ernest Spitzer ◽  
John N. A. Lott

The elemental composition of the protein bodies from several members of the Umbelliferae including carrot (Daucus carota L. cv. Imperator 408), wild carrot (Daucus carota L.), caraway (Carum carvi L.), anise (Pimpinella anisum L.), dill (Anethum graveolens L.), celery (Apium graveolens L. cv. Tall Utah), fennel (Foeniculum vulgare Mill.), parsnip (Pastinaca sativa L. cv. Hollow Crown), parsley (Petroselinum sativum L. cv. Moss Curled), and chervil (Anthriscus cerefolium L. cv. Curled) was determined using energy dispersive X-ray analysis. Globoid crystals in the endosperm usually contained P, K, and Mg or infrequently P, K, Mg, and Ca. In the embryos of carrot and caraway, P was always present with a combination of K, Mg, or Ca. Calcium was the only element detectable in the calcium-rich crystals. The proteinaceous matrix always contained S and K regardless of the inclusion present in the protein body.Quantitative determinations of P, Mg, K, and Ca are presented for all members studied except wild carrot, chervil, celery, and fennel. In all species analysed in this manner K or Ca were present in the highest amounts followed by P and Mg. Calcium-localization studies showed that most of the Ca is located in the endosperm and pericarp portions of the mericarp.

1982 ◽  
Vol 60 (8) ◽  
pp. 1399-1403 ◽  
Author(s):  
Ernest Spitzer ◽  
John N. A. Lott

The chemical composition of the calcium-rich crystal inclusions present in the seed protein bodies of carrot (Daucus carota L. cv. Imperator 408), wild carrot (Daucus carota L.), caraway (Carum carvi L.), anise (Pimpinella anisum L.), dill (Anethum graveolens L.), celery (Apium graveolens L. cv. Tall Utah), fennel (Foeniculum vulgare Mill.), parsnip (Pastinaca sativa L. cv. Hollow Crown), parsley (Petroselinum sativum L. cv. Moss Curled), and chervil (Anthriscus cerefolium L. cv. Curled) was determined. Using a variety of methods including X-ray diffraction, infrared spectroscopy, microincineration, energy dispersive X-ray analysis, solubility studies, and staining, the chemical composition of the calcium-rich crystal inclusions was identified as calcium oxalate.


1982 ◽  
Vol 60 (8) ◽  
pp. 1381-1391 ◽  
Author(s):  
Ernest Spitzer ◽  
John N. A. Lott

The structure of the protein bodies from seeds of the family Umbelliferae has not been studied extensively since late in the 19th century. Using light and electron microscopy structural aspects of the protein bodies of carrot (Daucus carota L. cv. Imperator 408), wild carrot (Daucus carota L.), caraway (Carum carvi L.), anise (Pimpinella anisum L.), dill (Anethum graveolens L.), celery (Apium graveolens L. cv. Tall Utah), fennel (Foeniculum vulgare Mill), parsnip (Pastinaca sativa L. cv. Hollow Crown), parsley (Petroselinum sativum L. cv.Moss Curled), and chervil (Anthriscus cerefolium L. cv. Curled) were studied. Both endosperm and embryo protein bodies were investigated. Structurally, the protein bodies from all these genera were similar in that two types of protein bodies were found. One type consisted of a homogeneous, proteinaceous matrix and a number of variously sized, globoid crystal inclusions. The other type consisted of a homogeneous, proteinaceous matrix and either an individual or, more commonly, an aggregate of calcium-rich crystals arranged in a cluster usually termed a druse. Both types of protein bodies were never found in the same cell. Only globoid crystals were found in the embryo protein bodies. Protein bodies in the embryos were smaller, more numerous per cell, and often contained a flocculent, proteinaceous matrix.


1982 ◽  
Vol 60 (8) ◽  
pp. 1404-1408 ◽  
Author(s):  
John N. A. Lott ◽  
Ernest Spitzer ◽  
Catherine M. Vollmer

Calcium mobilization into developing seedlings of several umbelliferous plants including carrot (Daucus carota L. cv. Imperator 408), wild carrot (Daucus carota L.), caraway (Carum carvi L.), anise (Pimpinella anisum L.), dill (Anethum graveolens L.), celery (Apium graveolens L. cv. Tall Utah), fennel (Foeniculum vulgare Mill.), parsnip (Pastinaca sativa L. cv. Hollow Crown), parsley (Petroselinum sativum L. cv. Moss Curled), and chervil (Anthriscus cerefolium L. cv. Curled) was investigated with emphasis on carrot and celery. Calcium determinations using atomic absorption spectrometry of carrot and celery embryos obtained from dry mericarps and carrot and celery seedlings collected when the pericarp plus testa plus endosperm remains fell off the seedlings, revealed that some calcium uptake occurred in carrot and possibly celery. It is possible that some of the calcium obtained by the seedlings came from the calcium oxalate crystals but the calcium could also have originated from other calcium sources within the endosperm. Polarized light studies of endosperm remains from carrot, celery, and the other members of the family Umbelliferae investigated, revealed that large numbers of calcium oxalate crystals were present in the endosperm remains after separation from the seedlings. The results of these studies indicate that calcium oxalate crystals are not used extensively as a calcium source during germination and early seedling growth.


Author(s):  
Alev ONDER ◽  
Suna Sibel GURPINAR, Mujde ERYILMAZ ◽  
Bayram Kagan AKAY, Ahsen Sevde CINAR

Spices are a part of the plants used for many purposes as preservatives and as colorants in foods or as medicinal intention. Main aim of the present research was to estimate the potential antimicrobial activity of some spices from Apiaceae family such as Amni visnaga (Diş otu, Hıltan), Anethum graveolens (Dereotu), Apium graveolens (Kereviz), Coriandrum sativum (Kişniş), Cuminum cyminum (Kimyon), Daucus carota (Havuç), Foeniculum vulgare (Rezene), Petroselinum sativum (Maydanoz), Pimpinella anisum (Anason). Thus, the fruits of the plants are used in the experiments. The fruits have been extracted by n-hexane, and all extracts have been subjected to TLC (Thin Layer Chromatography). The n-hexane extracts were screened for their potential in vitro antibacterial activity against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853 and antifungal activity against Candida albicans ATCC 10231 by microbroth dilution method. The hexane extracts of the fruits of Coriandrum sativum, Anethum graveolens, Daucus carota, and Pimpinella anisum did not show antimicrobial activity against tested microorganisms. Except these, the other extracts having MIC values of 2.5-5-10 mg/mL exhibited antimicrobial effect against some tested microorganisms. These results demonstrate that the extracts which have an antimicrobial effect can probably play a role as an antimicrobial agent owing to their nonpolar components which are accumulated to the n-hexane extracts.


2019 ◽  
pp. 1-3
Author(s):  
Alev ONDER ◽  
Suna Sibel GURPINAR, Mujde ERYILMAZ ◽  
Bayram Kagan AKAY, Ahsen Sevde CINAR

Spices are a part of the plants used for many purposes as preservatives and as colorants in foods or as medicinal intention. Main aim of the present research was to estimate the potential antimicrobial activity of some spices from Apiaceae family such as Amni visnaga (Diş otu, Hıltan), Anethum graveolens (Dereotu), Apium graveolens (Kereviz), Coriandrum sativum (Kişniş), Cuminum cyminum (Kimyon), Daucus carota (Havuç), Foeniculum vulgare (Rezene), Petroselinum sativum (Maydanoz), Pimpinella anisum (Anason). Thus, the fruits of the plants are used in the experiments. The fruits have been extracted by n-hexane, and all extracts have been subjected to TLC (Thin Layer Chromatography). The n-hexane extracts were screened for their potential in vitro antibacterial activity against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853 and antifungal activity against Candida albicans ATCC 10231 by microbroth dilution method. The hexane extracts of the fruits of Coriandrum sativum, Anethum graveolens, Daucus carota, and Pimpinella anisum did not show antimicrobial activity against tested microorganisms. Except these, the other extracts having MIC values of 2.5-5-10 mg/mL exhibited antimicrobial effect against some tested microorganisms. These results demonstrate that the extracts which have an antimicrobial effect can probably play a role as an antimicrobial agent owing to their nonpolar components which are accumulated to the n-hexane extracts.


Author(s):  
M. B. Ellis

Abstract A description is provided for Alternaria radicina. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Daucus carota, Apium graveolens, Petroselinum crispum, Pastinaca sativa, Anethum graveolens. DISEASE: Black rot of carrot (Daucus carota); also attacks celery (Apium graveolens), parsley (Petroselinum crispum), parsnip (Pastinaca sativa) and dill (Anethum graveolens) (15: 275; 30: 140). Causes pre- emergence and post-emergence damping-off; primary lesions of older plants begin at the base of the petiole from where a dark, usually shallow, lesion spreads into the crown and sides of the root. Secondary lesions develop below ground and are often coincident with cracks and splits. A dry, mealy rot may develop in storage. Necrotic lesions occur on the inflorescence and occasionally on the older leaves but the leaf blight is probably not as severe as that caused by A. dauci (Kuhn) Groves & Skolko. GEOGRAPHICAL DISTRIBUTION: Africa (Nigeria), Americas (Argentina, Canada, USA), Asia (India, Israel, Japan), Australia and Europe (Britain, Bulgaria, Czechoslovakia, Denmark, Finland France, Germany, Hungary, Italy, Netherlands, Norway, Rumania, Sweden, USSR). TRANSMISSION: Seed-borne and soil-borne (12: 72; 22: 339; 26: 405; 44, 948; 46, 1160); persists in the soil for up to 6 yr (47, 3299i; 49, 3555d).


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