Seed development in Ricinus communis cv. Hale (castor bean). III. Pattern of storage protein and phytin accumulation in the endosperm
The development of the endosperm of castor bean seed from its initial free nuclear state through to the end of maturation is presented. An investigation of the pattern of reserve accumulation in the endosperm at the light microscopy level revealed that the accumulation of soluble and insoluble storage proteins, and of phytin, does not occur simultaneously in all cells of the developing storage organ. Rates of reserve accumulation also vary among regions of the endosperm. Storage protein and phytin accumulation are initiated in a region midway between the periphery and central lumen of the endosperm by the early cotyledon stage of seed development. Afterwards, reserve deposition occurs more intensely in the proximal and more peripheral regions than in the distal and internal regions. A wave of reserve accumulation, or protein body maturation, proceeds from the more peripheral and the proximal regions to the more internal and distal regions as development continues. The last cells to complete reserve deposition are those in regions lying close to the endosperm lumen (into which the cotyledons have expanded) and the outermost two cell layers of the endosperm.