Induction of basidiospore germination by gluconic acid in the ectomycorrhizal fungus Tricholoma robustum

1992 ◽  
Vol 70 (6) ◽  
pp. 1234-1238 ◽  
Author(s):  
Koji Iwase
Keyword(s):  
Key Words ◽  
Conditioned Medium ◽  
Ectomycorrhizal Fungi ◽  
Related Species ◽  
Gluconic Acid ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Mycelial Culture

Basidiospore germination in the ectomycorrhizal fungus Tricholoma robustum and related species Tricholoma matsutake, Tricholoma caligatum, and Tricholoma ponderosum was examined. Basidiospore germination in T. robustum was induced by the presence of conspecific mycelium but not by the mycelium of related species. Germination also occurred on conditioned medium prepared by preculturing with conspecific mycelium. Gluconic acid, which is produced by T. robustum, also induced basidiospore germination in that species. After storage at 5 °C, germination of basidiospores in T. robustum was stimulated by incubation with conspecific mycelium but not with gluconic acid. These findings suggest that gluconic acid is but one of perhaps many germination-inducing substances produced by the mycelium of T. robustum. Key words: basidiospore germination, ectomycorrhizal fungi, gluconic acid, mycelial culture, Tricholoma robustum.

Gluconic acid synthesis by the ectomycorrhizal fungus Tricholoma robustum

1992 ◽  
Vol 70 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Koji Iwase
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
High Performance ◽  
Culture Medium ◽  
Acid Synthesis ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Mycelial Culture ◽  
High Productivity ◽  
Tricholoma Bakamatsutake

During mycelial culture of Tricholoma robustum, the medium gradually became acidified to approximately pH 3.9. High performance liquid chromatography showed that gluconic acid was secreted into the culture medium, and the amount of gluconic acid produced was measured by enzymatic analysis. Gluconic acid synthesis by all other related species, Tricholoma matsutake, Tricholoma caligatum, Tricholoma ponderosum, Tricholoma fulvocastaneum, and Tricholoma zelleri was poor, except for Tricholoma bakamatsutake, which showed relatively high productivity. Activity of glucose oxidase, which is responsible for gluconic acid production, was highest in T. robustum and second highest in T. bakamatsutake. The activity in these two species was much higher than those of other species. These results indicate that gluconic acid was synthesized from glucose by glucose oxidase in T. robustum as well as in T. bakamatsutake. Key words: ectomycorrhizal fungi, gluconic acid, glucose oxidase, Tricholoma robustum.

scholarly journals The Influence of Microfungi on the Mycelial Growth of Ectomycorrhizal Fungus Tricholoma matsutake

2019 ◽  
Vol 7 (6) ◽  
pp. 169 ◽  
Author(s):  
Seung-Yoon Oh ◽  
Myung Soo Park ◽  
Young Woon Lim
Keyword(s):  
Molecular Markers ◽  
Ectomycorrhizal Fungi ◽  
Mycelial Growth ◽  
Slow Growth ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Forest Nursery ◽  
Promoting Effect ◽  
Trichoderma Species ◽  
Fungal Isolates

Pine mushroom (Tricholoma matsutake) is one of the most valued ectomycorrhizal fungi in Asia because of its unique pine-like aroma; however, due to exceptionally slow growth of its mycelia in artificial conditions, its cultivation has been largely deemed as not possible. Previous studies have shown that some bacteria and a few Trichoderma species associated with pine mushroom promoted the growth of T. matsutake isolate, but this effect is relatively unexplored. In this study, we investigated the diversity of microfungi in the fairy ring of T. matsutake and their effect on the growth of T. matsutake isolate. From 184 fungal isolates, 28 species were identified based on suitable molecular markers. Penicillium was most frequently observed (16 species), followed by Trichoderma (4 species). Five Zygomycota species showed a high promoting effect on the growth of T. matsutake while the effects of ascomycetes were mixed. The microfungi that promote the growth of T. matsutake can be useful for forest nursery and artificial cultivation of T. matsutake.

Detection of the ectomycorrhizal fungus Tricholoma matsutake and some related species with specific ITS primers

Mycoscience ◽  
2000 ◽  
Vol 41 (1) ◽  
pp. 33-37 ◽  
Author(s):  
William Alexander Dunstan ◽  
Bernard Dell ◽  
Nicholas Malajczuk ◽  
Koji Iwase
Keyword(s):  
Related Species ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Its Primers

scholarly journals Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungi

Sommerfeltia ◽  
2008 ◽  
Vol 31 (1) ◽  
pp. 161-177 ◽  
Author(s):  
U. Peintner
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Related Species ◽  
Dry Weight ◽  
Species Concepts ◽  
Its Sequences ◽  
Phylogenetic Species ◽  
Closely Related Species ◽  
Rdna Its ◽  
Rdna Its Sequences ◽  
Distinguishing Character

Cortinarius alpinus as an example for morphological and phylogenetic species concepts in ectomycorrhizal fungiExtensive morphological and molecular analyses of closely related species from alpine, subalpine and montane habitats should enable a comparison of ecological, morphological and phylogenetic species concepts in ectomycorrhizal mushrooms. One fundamental question of this study was whether alpine species really exist, and which criteria, besides the specific habitat, could reliably be used for the de-limitation of such taxa. For this reason, 56 rDNA ITS sequences were generated or downloaded from GenBank for 10 closely related species of Cortinarius subgenus Myxacium, section Myxacium. Several collections were sequenced for each of the following taxa: Cortinarius absarokensis, C. alpinus, C. favrei, C. fennoscandicus, C. grallipes, C. mucosus, C. muscigenus, C. septentrionalis, C. trivialis and C. vernicosus. Moreover, spore statistics were carried out for 38 collections of alpine and subalpine taxa. These data provide clear evidence for C. favrei being a synonym of C. alpinus. C. absarokensis and C. alpinus can clearly be delimited based on pileus diameter and average dry weight per basidiome, even in overlapping habitats, but spore size and shape is not a good distinguishing character. Phylograms have very short branches, and base differences between ITS sequences are generally very low in this group, and give no resolution for the included taxa of this section. Based on these results, species concepts of ectomycorrhizal mushrooms are discussed in detail.

scholarly journals Metallothionein-like peptides involved in sequestration of Zn in the Zn-accumulating ectomycorrhizal fungus Russula atropurpurea

Metallomics ◽  
2014 ◽  
Vol 6 (9) ◽  
pp. 1693-1701 ◽  
Author(s):  
Tereza Leonhardt ◽  
Jan Sácký ◽  
Pavel Šimek ◽  
Jiří Šantrůček ◽  
Pavel Kotrba
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Ectomycorrhizal Fungus ◽  
Substantial Portion ◽  
Natural Conditions ◽  
Binding Peptides ◽  
Russula Atropurpurea

The first evidence of the existence of gene-encoded Zn-binding peptides that sequester a substantial portion of intracellular Zn in ectomycorrhizal fungi under natural conditions.

scholarly journals Cadmium-Responsive Thiols in the Ectomycorrhizal Fungus Paxillus involutus

2004 ◽  
Vol 70 (12) ◽  
pp. 7413-7417 ◽  
Author(s):  
Mikael Courbot ◽  
Laurent Diez ◽  
Roberta Ruotolo ◽  
Michel Chalot ◽  
Pierre Leroy
Keyword(s):  
Molecular Mass ◽  
Ectomycorrhizal Fungi ◽  
High Performance ◽  
Metal Tolerance ◽  
Ectomycorrhizal Fungus ◽  
Paxillus Involutus ◽  
Cellular Mechanisms ◽  
Chromatography Method ◽  
Relative Lack ◽  
Liquid Chromatography Method

ABSTRACT Molecular and cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives (γ-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and γ-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.

scholarly journals Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence

Mycorrhiza ◽  
2018 ◽  
Vol 29 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Lu-Min Vaario ◽  
Shambhu Prasad Sah ◽  
Mariko Norisada ◽  
Maki Narimatsu ◽  
Norihisa Matsushita
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Tricholoma Matsutake ◽  
Organic Form ◽  
Isotopic Evidence

scholarly journals KARAKTERISTIK AKAR BEREKTOMIKORIZA PADA Shorea pinanga, Pinus merkusii DAN Gnetum gnemon

PERENNIAL ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 11
Author(s):  
Melya Riniarti ◽  
Irdika Mansur ◽  
Arum Sekar Wulandari ◽  
Cecep Kusmana
Keyword(s):  
Key Words ◽  
Ectomycorrhizal Fungi ◽  
Host Plants ◽  
Clamp Connection ◽  
The Other ◽  
Branching Pattern ◽  
Root Tips ◽  
Hartig Net ◽  
Gnetum Gnemon ◽  
Pinus Merkusii

Morphology and anatomy characteristics often used to identify ectomycorrhizal fungi. We used three Scleroderma spp. (Scleroderma columnare, S. dictyosporum), and S. sinnamariense) and inoculated to Shorea pinanga, Pinus merkusii, and Gnetum gnemon. After 6,8, and 10 months, each root tips were collected to determined hyphae colour, branching pattern, clamp-connection, hartig net and mantle. This result revealed that S. sinnamariense did not form association with S. pinanga and P. merkusii but form association with G. gnemon. On the other hand, S. columnare and S. dictyosporum could form association with all the host plants. S. columnare and S. dictyosporum formed white hyphae while S. sinnamariense formed yellow hyphae with monopodial branching pattern. The depth of hartig net and mantle was increased by timed. Key words: ectomycorrhizal fungi, hartig net, mantle, Scleroderma

Relationship between climate, expansion rate, and fruiting in fairy rings (‘shiro’) of an ectomycorrhizal fungus Tricholoma matsutake in a Pinus densiflora forest

2015 ◽  
Vol 15 ◽  
pp. 18-28 ◽  
Author(s):  
Maki Narimatsu ◽  
Toshiyuki Koiwa ◽  
Takashi Masaki ◽  
Yuichi Sakamoto ◽  
Hisao Ohmori ◽  
...  
Keyword(s):  
Pinus Densiflora ◽  
Ectomycorrhizal Fungus ◽  
Expansion Rate ◽  
Tricholoma Matsutake ◽  
Fairy Rings ◽  
Pinus Densiflora Forest
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