Sensitivity of Populus tremuloides to toxic metabolites of Hypoxylon mammatum and susceptibility to H. mammatum infection

1993 ◽  
Vol 71 (10) ◽  
pp. 1298-1303 ◽  
Author(s):  
Brian M. Kruger ◽  
Paul D. Manion
Keyword(s):  
Tissue Culture ◽  
Populus Tremuloides ◽  
Significant Variation ◽  
Factors Influencing ◽  
Toxic Metabolites ◽  
Disease Condition ◽  
In Vitro Inoculation ◽  
Hypoxylon Mammatum ◽  
Growth Of Fungi

The response of 17 aspen tissue culture clones to bioassays with metabolites of Hypoxylon mammatum and in vitro inoculation with H. mammatum ascospores was determined. Although statistically significant variation among aspen clones was observed in response to both bioassays, response of the clones to the assays was not related (r = 0.361). This suggests that H. mammatum metabolites are not essential for the infection of tissue cultured aspen. Forty plantation-grown aspen differing in response to natural H. mammatum infection were also tested for sensitivity to metabolites of H. mammatum. Large differences in the sensitivity of the clones to the metabolites were observed, but these differences were not related to the disease condition of the clones. The toxic metabolites isolated from H. mammatum had no effect on the growth of fungi isolated from healthy aspen stems. Overall, these results suggest that the sensitivity of aspen to H. mammatum toxic metabolites is only one of the several factors influencing susceptibility. Key words: Populus tremuloides, Hypoxylon mammatum, phytotoxin.

Genetic control of Populus tremuloides sensitivity to metabolites of Hypoxylon mammatum

1993 ◽  
Vol 71 (10) ◽  
pp. 1276-1279 ◽  
Author(s):  
Brian M. Kruger ◽  
Paul D. Manion
Keyword(s):  
Tissue Culture ◽  
Key Words ◽  
Genetic Control ◽  
Analysis Of Variance ◽  
Populus Tremuloides ◽  
Culture Filtrates ◽  
Number Of Genes ◽  
Hypoxylon Mammatum ◽  
Main Effects ◽  
Tissue Culture Plantlets

Tissue culture plantlets derived from the vegetative buds of six aspen trees and from the seeds of controlled pollinations of these trees were bioassayed with culture filtrates from three isolates of Hypoxylon mammatum. Analysis of variance of parent and progeny bioassay responses revealed significant main effects and insignificant interaction, which suggests that metabolite sensitivity is a horizontally controlled trait. However, in certain crosses progeny response differed markedly from that of either parent. In these cases progeny were either far more or far less sensitive than the parents. This dramatic switching of sensitivity to the metabolites suggests that this trait is conditioned by a small number of genes. Key words: Populus tremuloides, Hypoxylon mammatum, phytotoxin.

Amino acid content of water-stressed plantlets of Populus tremuloides clones in relation to clonal susceptibility to Hypoxylon mammatum in vitro

1990 ◽  
Vol 68 (1) ◽  
pp. 26-29 ◽  
Author(s):  
R. R. Bélanger ◽  
P. D. Manion ◽  
D. H. Griffin
Keyword(s):  
Amino Acids ◽  
Water Stress ◽  
Amino Acid ◽  
Populus Tremuloides ◽  
Acid Content ◽  
Amino Acid Content ◽  
Susceptibility To Infection ◽  
Hypoxylon Mammatum ◽  
Inoculation Study

Plantlets from five clones of Populus tremuloides were cultured in media at three different osmotic potentials to assess the effects of water stress on amino acid content. Differences in amino acid content were then related to differences in susceptibility (determined in a previous study) of clones to Hypoxylon mammatum. Changes in amino acid concentrations varied among the amino acids. Alanine, arginine, asparagine, glutamine, glutamate, and proline increased generally but not consistently among all clones. Comparisons among the clones for these six amino acids showed significant variations. Proline, which has been shown to accelerate radial growth of H. mammatum more than other amino acids, increased progressively with increased water stress in all clones. Levels of proline among the clones were correlated (r = 0.89) with susceptibility to infection and colonization of the clones by H. mammatum. Alanine and glutamine were also similarly correlated with infection rating. However, one clone, which grew poorly in culture, did not show this relationship. In comparison with the other clones, it had low levels of all amino acids, which did not increase significantly in response to water stress. Yet in the inoculation study, it had the highest infection rating at all stress levels, suggesting that other stress-induced factors are also important. These results suggest that nitrogen status of some aspen clones under water stress may increase their susceptibility to hypoxylon canker.

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jyothi R ◽  
Srinivasa Murthy K M ◽  
Hossein . ◽  
Veena .
Keyword(s):  
Tissue Culture ◽  
Wide Distribution ◽  
Colocasia Esculenta ◽  
Limiting Factor ◽  
Promising Alternative ◽  
Rapid Multiplication ◽  
Medical Plant ◽  
Total Fat ◽  
Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of  tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

1959 ◽  
Vol XXXII (I) ◽  
pp. 41-53 ◽  
Author(s):  
Stig Kullander ◽  
Bengt Källén
Keyword(s):  
Tissue Culture ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

Factors Influencing in Vitro Rumen Cellulose Digestion

1956 ◽  
Vol 15 (4) ◽  
pp. 1180-1187 ◽  
Author(s):  
C. N. Huhtanen ◽  
R.F. Elliott
Keyword(s):  
Cellulose Digestion ◽  
Factors Influencing

Factors Influencing Development of Wheat‐Rye Hybrid Embryos in Vitro 1

Crop Science ◽  
1978 ◽  
Vol 18 (2) ◽  
pp. 348-350 ◽  
Author(s):  
T. Tiara ◽  
E. N. Larter
Keyword(s):  
Factors Influencing

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant
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