Impact of insect root herbivory on the growth and nitrogen and carbon contents of white clover (Trifolium repens) seedlings

1996 ◽  
Vol 74 (10) ◽  
pp. 1591-1595 ◽  
Author(s):  
P. J. Murray ◽  
D. J. Hatch ◽  
J. B. Cliquet

The impact of root herbivory by larvae of the weevil Sitona flavescens (Marsh.) on the growth and carbon and nitrogen economies of seedlings of white clover (Trifolium repens L.) and the nature and extent of nitrogenous compounds found in the root exudates taken from the rhizosphere were investigated. The seedlings were grown in sand culture in a system of microlysimeters that enabled sequential sampling of root exudates. Weevil infestation significantly reduced foliar biomass and total N and C contents and impaired N-fixation. The C:N ratios of the infested plants were significantly increased compared with those of uninfested seedlings. The most abundant amino acids in the exudates were aspartic acid and serine. Keywords: nitrogen fixation, Sitona spp., root exudates, white clover.

1959 ◽  
Vol 10 (4) ◽  
pp. 500 ◽  
Author(s):  
K Spencer

Plants of white clover (Trifolium repens L. var. Ladino) were grown in sand culture with four levels of sulphur supply. Growth increased with increasing sulphur supply, all plants except those at the highest sulphur level showing deficiency symptoms. As the severity of the deficiency increased, the root system formed a proportionately larger part of the plant, and the stems and petioles smaller proportions; the proportion of the whole plant formed by the laminae was reduced to only a slight extent. Nitrogen and sulphur fractions were examined in the laminae, nitrogen at each of three harvests and sulphur at the second harvest. The percentages of protein nitrogen and of total nitrogen increased as sulphur supply increased, protein nitrogen forming a greater proportion of the total nitrogen at the higher sulphur levels. In contrast, protein sulphur formed the bulk of the total sulphur in deficient plants, but as sulphur supply approached an adequate level for growth, there was a marked increase in non-protein organic sulphur and a smaller increase in sulphate sulphur. In this respect, white clover appears to differ from legumes other than Trifolium spp. and from non-legumes, all of which accumulate sulphur mainly as sulphate.


2002 ◽  
Vol 55 ◽  
pp. 111-115 ◽  
Author(s):  
J.P.J. Eerens ◽  
S.S. Seefeldt ◽  
G. Garry ◽  
M.L. Armstrong

The impact of grazing pasture to different heights on the development of Californian thistle (Cirsium arvense) was simulated over two years in container trials Californian thistle field densities were simulated by transplanting seedlings into perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) that had been sown at 20 and 5 kg/ha respectively In a high fertility situation adding fertiliser did not influence Californian thistle development in the pasture sward Californian thistle grown without companion ryegrass and white clover grew the best reaching the highest levels of biomass (shoot and root material) and number of shoots (supra and subterranean) Californian thistle grown with the highest level of pasture competition fared the worst with the lowest level of biomass and number of shoots The results show that grazing to a higher residual severely reduces Californian thistle growth and is a useful tool to assist in the control of Californian thistle


2014 ◽  
Vol 65 (4) ◽  
pp. 382 ◽  
Author(s):  
S. N. Nichols ◽  
J. R. Crush ◽  
L. Ouyang

Previous studies in sand culture suggested that some white clover (Trifolium repens) × T. uniflorum interspecific hybrids were more tolerant than white clover of low external phosphate (P) supply. Here, P acquisition and growth responses were determined in soil for two T. repens × T. uniflorum backcross hybrids and their parental white clover cultivar, grown in a glasshouse pot experiment at Olsen P of 6, 7, 9, 14, or 20 mg P kg–1 soil. Growth of all of the clover entries responded strongly to increasing soil P levels, and one hybrid clover grew, on average, 17% better than the white clover control cultivar at Olsen soil P 9–20 mg kg–1. Internal P concentrations and shoot growth per unit P absorbed did not differ among the clovers. Instead, improved growth of the hybrid resulted from a greater ability to acquire soil P. This hybrid had the longest, most frequently branched roots. Frequent branching and growth of root tips into fresh soil would reduce the limitations to P uptake imposed by slow diffusion of P to the root surface. The results confirm previous observations that interspecific hybridisation is a useful strategy for increasing the range of P responsiveness in breeding populations for white clover.


2013 ◽  
Vol 10 (12) ◽  
pp. 8269-8281 ◽  
Author(s):  
T. Watanabe ◽  
S. Bowatte ◽  
P. C. D. Newton

Abstract. Using the δ15N natural abundance method, we found that the fraction of nitrogen derived from atmospheric N (%Ndfa) in field-grown white clover (Trifolium repens L.) plants was significantly lower (72.0% vs. 89.8%, p = 0.047 in a grassland exposed to elevated CO2 for 13 yr using free air carbon dioxide enrichment (FACE). Twelve months later we conducted an experiment to investigate the reasons behind the reduced N fixation. We took cuttings from white clover plants growing in the FACE and established individual plants in a glasshouse using soil from the appropriate ambient or elevated CO2 treatments. The established plants were then transplanted back into their "rings of origin" and sampled over a 6-week period. We used molecular ecological analyses targeting nifH genes and transcripts of rhizobia in symbiosis with white clover (Trifolium repens L.) to understand the potential mechanisms. Shoot biomass was significantly lower in eCO2, but there was no difference in nodule number or mass per plant. The numbers of nifH genes and gene transcripts per nodule were significantly reduced under eCO2, but the ratio of gene to transcript number and the strains of rhizobia present were the same in both treatments. We conclude that the capacity for biological nitrogen fixation was reduced by eCO2 in white clover and was related to the reduced rhizobia numbers in nodules. We discuss the finding of reduced gene number in relation to factors controlling bacteroid DNA amount, which may imply an influence of nitrogen as well as phosphorus.


2006 ◽  
Vol 57 (5) ◽  
pp. 577 ◽  
Author(s):  
M. L. Castle ◽  
J. R. Crush ◽  
J. S. Rowarth

Limited information is available on the factors influencing the uptake and distribution of nitrogen (N) at low temperatures. This experiment quantified the distribution of N in white clover at root and shoot temperatures of 8°C or 24°C. Stolon tip cuttings of white clover (Trifolium repens L.) were grown in silica sand. After 62 days, plants were transferred to an 8°C or 24°C controlled environment room and, to quantify the distribution of N, a pulse of 15KNO3 was applied. Thereafter, plants were supplied with a complete nutrient solution containing NH4NO3 at a concentration calculated to provide plants with 20% of their N requirement. Plants were harvested at 0, 1, 4, 8, 24, 168, or 336 h. Leaf area and dry weights plus 14N/15N distribution in all fractions and total N concentration were measured. At both temperatures, the dry weights in all fractions increased significantly (P < 0.05) with time. After 336 h the amount of labelled 15N contained in the laminae and petioles was lower at 8°C than at 24°C. The higher 15N recovery in the laminae and petioles, and the higher lamina N%, indicated more N had been transported from the roots to the laminae at 24°C. This investigation suggests that temperature does affect the movement of N around the plant, with a consequent effect on N pool sizes and, hence, growth.


Author(s):  
D.R. Woodfield ◽  
J.R. Caradus

Better persistence and reliability of white clover (Trifolium repens L.) is critical in achieving a more competitive New Zealand farming industry. To persist, white clover must establish well and withstand the accumulated stresses of competition from associated grasses, grazing, variable soil fertility, drought, plus pest and disease pressure. These factors vary markedly with environment and farming system, making the choice of appropriate grazing management, plant nutrition, companion species and cultivar difficult. White clover is particularly vulnerable to mismanagement and environmental stresses during spring when plant size is at its smallest. This vulnerability is further compounded by the current trend in dairying to apply high rates of nitrogen (N) which favours grass growth more than clover growth. A faster grazing rotation and/or higher stocking rates can offset the adverse effects of N on white clover by utilising the additional grass produced and reducing competition for light. Irrespective of N inputs, frequent defoliation during spring favours white clover persistence by increasing grass tiller density, resulting in better ground cover and in lower soil surface temperatures in summer. There is a threshold above which the density of associated grass suppresses clover growth. This is most prevalent in swards containing browntop, cocksfoot and kikuyu, which are more competitive against white clover than tall fescue, timothy and perennial ryegrass. Plant breeding efforts to improve persistence concentrate on increasing the rate of stolon formation and decreasing the rate of stolon death. These efforts include selecting genotypes that have better spread and persistence in association with different grasses, and genotypes that continue to grow with lower inputs of phosphate. Changes in root morphology have enhanced persistence under moderate drought stress, while significant improvements in resistance to clover cyst nematode, root-knot nematode and clover flea offer real hope in reducing the impact of these pests. Developing cultivars with higher stolon growing point densities at a particular leaf size should improve persistence while maintaining the greater yield potential. Keywords: climatic stresses, competition, diseases, grazing management, pests, plant breeding, plant nutrition, Trifolium repens L.


2013 ◽  
Vol 10 (6) ◽  
pp. 9867-9896 ◽  
Author(s):  
T. Watanabe ◽  
S. Bowatte ◽  
P. C. D. Newton

Abstract. Using the δ15N natural abundance method, we found that the fraction of nitrogen derived from atmospheric N (%Ndfa) in field grown white clover (Trifolium repens L.) plants was significantly lower (72.0% vs. 89.5%, p = 0.047 in a grassland exposed to elevated CO2 for 13 yr using Free Air Carbon Dioxide Enrichment (FACE). Twelve months later we conducted an experiment to investigate the reasons behind the reduced N fixation. We took cuttings from white clover plants growing in the FACE and established individual plants in a glasshouse using soil from the appropriate ambient or elevated CO2 treatments. The established plants were then transplanted back into their "rings of origin" and sampled over a 6 week period. We used molecular ecological analyses targeting nifH genes and transcripts of rhizobia in symbiosis with white clover (Trifolium repens L.) to understand the potential mechanisms. Shoot biomass was significantly lower in eCO2 but there was no difference in nodule number or mass per plant. The numbers of nifH genes and gene transcripts per nodule were significantly reduced under eCO2 but the ratio of gene to transcript number and the strains of rhizobia present were the same in both treatments. We conclude that the capacity for biological nitrogen fixation was reduced by eCO2 in white clover and was related to the reduced rhizobia numbers in nodules. We discuss the finding of reduced gene number in relation to factors controlling bacteroid DNA amount which may imply an influence of nitrogen as well as phosphorus.


2014 ◽  
Vol 65 (4) ◽  
pp. 370 ◽  
Author(s):  
S. N. Nichols ◽  
R. W. Hofmann ◽  
W. M. Williams ◽  
J. R. Crush

Interspecific hybridisation is being utilised in white clover (Trifolium repens L.) breeding programs to overcome factors currently restricting productivity and persistence. Valuable new traits that may be introduced from the wild relative T. uniflorum include root characteristics and other adaptations to its natural, Mediterranean habitat. This study examined the effect of hybridisation on growth and macronutrient composition of white clover compared with T. uniflorum and T. repens × T. uniflorum backcross 1 (BC1) hybrids in two glasshouse sand culture experiments. Shoot and root dry weights of BC1 hybrids were greater than of white clover in low-concentration nutrient treatments but not in a more concentrated treatment. Decreases in dry weight with decreasing nutrient treatment strength were also smaller for some BC1 hybrids compared with white clover and other hybrid families. Most foliar macronutrient levels were adequate for white clover growth, but mean shoot or leaf phosphorus (P) concentrations were below published critical levels. Higher dry matter production under these low internal P concentrations suggests that some T. repens × T. uniflorum BC1 hybrids may be more tolerant of lower soil P levels than white clover. Such adaptations are likely to have been inherited from T. uniflorum. However, transgressive segregation may also be occurring, as T. uniflorum was larger than white clover in some, but not all, cases of low nutrient supply.


Author(s):  
R.N. Watson ◽  
C.F. Mercer

This paper reviews current research within AgResearch to reduce the impact of plant-feeding nematodes. Plant-feeding nematodes reduce pasture production by around 15% annually, mainly through their effect on white clover. Nematicide application increases clover yields in pasture by an average 40% and N-fixation levels by over 50%. The problem occurs nationally. When pasture nematode burdens are reduced in established or newly sown ryegrass-white clover pasture, white clover can generally assume dominance during periods of active plant growth. The impact of clover nematodes in reducing nitrogen inputs and forage quality is estimated to exceed $1 billion annually in lost production potential. A gain of 1% in clover performance applied nationally is estimated to be worth up to $48 million. The research programme on pasture nematodes conducted within AgResearch has included evaluation of management practices that may reduce nematode impacts, selection of white clover seedlines for resistance or tolerance to nematodes, and identification of agents for biological control of nematodes within New Zealand pastures. Keywords: clover nematodes, Heterodera trifolii, impacts, Meloidogyne trifoliophila, M. hapla, Pratylenchus spp., Trifolium repens


Author(s):  
K.H. Widdup ◽  
D.R. Woodfield ◽  
I.J. Baird ◽  
P.T.P. Clifford

The successful commercialisation of agronomically superior white clover (Trifolium repens) cultivars is dependent on their seed production potential. Field trials were established in 2000 and repeated in 2003 to determine the impact of selection for increased seed yield. The seed yield of the pre-release cultivar (the base population prior to selection for seed yield and cultivar release, representing generation 0) was compared with the field Nucleus generation of the released cultivar (second generation after selection for seed yield, representing generation 2) and the Basic seed generation (the fourth generation after selection for seed yield, representing generation 4), in six recent cultivars (Grasslands Kopu II, Grasslands Challenge, NuSiral, Grasslands Sustain, Grasslands Demand and Grasslands Prestige). The Nucleus and Basic generations had consistently higher seed yield than the pre-release generation for all cultivars. These increases in seed yield were associated with increases in inflorescence density (inflorescences/m2) and to a lesser extent with increased seed yield/inflorescence. Changes were also evident in flowering pattern with four of the six cultivars having a sharper flowering peak following selection. This improved uniformity was also evident through reduced variability in leaf size of the Nucleus generation compared to the pre-release generation. Grasslands Kopu II had the highest seed yields which were associated with moderately high inflorescence density in combination with high seed yield/ inflorescence. The selection strategy used in the final phase of the development of these cultivars has proven successful in increasing seed yield through refining flowering pattern and production while maintaining the morphology and uniformity of the new cultivar. Keywords: cultivars, genetic improvement, seed production, Trifolium repens, white clover


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