Leaf secretory tissues in Myrsine coriacea and Myrsine venosa (Primulaceae): ontogeny, morphology, and chemical composition of essential oils

Secretory structures are an outstanding feature in Primulaceae (Ericales). Such structures are known for their taxonomical and medicinal importance. However, a detailed morphological study of the secretory structures in Primulaceae has been neglected. Selected species for this study belong to Myrsine, a widely distributed genus in Brazil, popularly known as “capororoca”. In this study, we aimed to elucidate the ontogenesis of the secretory structures in the leaves of Myrsine coriacea (Sw.) R. Br. ex Roem & Schult. and Myrsine venosa A.DC. and report, for the first time, on the composition of their essential oils. The following secretory structures are found in M. coriacea and M. venosa: idioblasts, glandular trichomes, and secretory cavities. The development of all secretory structures, which is asynchronous, occurs during leaf expansion and differentiation; therefore, in leaf primordia, the same type of secretory structure could be observed at different stages of differentiation. By the complete expansion of leaf primordia, all secretory structures have reached their full size. Idioblasts are derived from both protodermal and ground meristem cells and they secrete mucilage or phenolic compounds. The glandular trichomes can be peltate, as found in both species, or branched, as found only in M. coriacea. Trichomes are initiated by the enlargement of protodermal cells, followed by their division, and they are completely formed by the end of leaf expansion. Secretory cavities are schizogenous and originated from ground meristem cells. Major components from M. coriacea essential oils were β-elemene, γ-muurolene, and α-cadinene, while the major components of M. venosa essential oils were β-caryophyllene, γ-muurolene, and δ-cadinene.

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Composition of Essential Oils from Leaves and Flowers of Stachys Germanica Subsp. Salviifolia (Ten.) Gams (Labiatae) and Related Secretory Structures

Natural Product Communications ◽

10.1177/1934578x0900400618 ◽

2009 ◽

Vol 4 (6) ◽

pp. 1934578X0900400 ◽

Cited By ~ 4

Author(s):

Claudia Giuliani ◽

Roberto Maria Pellegrino ◽

Bruno Tirillini ◽

Laura Maleci Bini

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Glandular Trichomes ◽

Secretory Structures ◽

Epidermal Surface ◽

Germacrene D ◽

Different Types ◽

Leaf Oil ◽

Leaf Essential Oil ◽

First Time

The essential oils from both leaves (L) and flowers (F) of Stachys germanica subsp. salviifolia (Ten.) Gams (Labiatae/Lamiaceae) from Italy were analyzed by GC-MS for the first time. The distribution, morphology and histochemistry of the different types of glandular trichomes present on the epidermal surface were also determined. Twenty-nine constituents, representing 89.4% of the total leaf oil, and forty-one compounds, forming 97.8% of the total flower oil, were identified. Germacrene D (39.4%), phytol (10.2%), β-bourbonene (3.5%) and β-ylangene (3.3%) were recognized as the main constituents of the leaf essential oil, while limonene (24.1%), β-pinene (18.7%), germacrene D (12.8%) and ( E)-nerolidol (6.6%) were the main compounds of the flower essential oil.

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Chemical composition and antifungal activity of essential oils from four Asteraceae plants grown in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2017-0219 ◽

2018 ◽

Vol 73 (7-8) ◽

pp. 313-318 ◽

Cited By ~ 4

Author(s):

Rose Vanessa Bandeira Reidel ◽

Simona Nardoni ◽

Francesca Mancianti ◽

Claudia Anedda ◽

Abd El-Nasser G. El Gendy ◽

...

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Activity ◽

First Time

Abstract The objective of the present paper was the assessment of the chemical composition of the essential oils from four Asteraceae species with a considerable food, medicinal, and agricultural value, collected in Egypt, together with their in vitro inhibitory activity against molds and yeasts. The essential oil of Launaea cornuta flowers was also evaluated for the first time, but because of its very low yield (<0.01%), no antifungal test was performed.

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Intraspecific Variability of the Essential Oil of Cladanthus mixtus from Morocco

Natural Product Communications ◽

10.1177/1934578x1400900137 ◽

2014 ◽

Vol 9 (1) ◽

pp. 1934578X1400900

Author(s):

Anass Elouaddari ◽

Abdelaziz El Amrani ◽

Jamal JamalEddine ◽

José G. Barroso ◽

Luis G. Pedro ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Intraspecific Variability ◽

Individual Plant ◽

Aerial Parts ◽

Plant Samples ◽

First Time

Air-dried aerial parts of wild Cladanthus mixtus were collected from two different regions of Morocco, Bouznika and Oujda, during 2011 and 2012. Forty individual plant samples were hydrodistilled using a Clevenger apparatus and the obtained essential oils were analyzed by GC and GC/MS. The yield obtained varies greatly with a range of 0.3 to 0.8%. The chemical composition of C. mixtus oils changes from one region to another. A total of 53 constituents were identified. To the best of our knowledge, two chemotypes were defined for the first time for this species in the regions studied. 2-Methyl-2- trans-butenyl methacrylate (32.8–35.2%) / ar-curcumene (13–14%) characterize the chemotypeof the C. mixtus plants from Bouznika, and trans-β-farnesene (35.5–50.3%) the chemotype from Oujda.

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Phylogenetic implications of the anatomical study of the Amburaneae clade (Fabaceae: Faboideae)

Botanical Journal of the Linnean Society ◽

10.1093/botlinnean/boaa019 ◽

2020 ◽

Vol 194 (1) ◽

pp. 69-83

Author(s):

João Pedro Silvério Pena Bento ◽

Edna Scremin-Dias ◽

Flávio Macedo Alves ◽

Vidal De Freitas Mansano ◽

Ângela Lúcia Bagnatori Sartori

Keyword(s):

Vascular System ◽

Phylogenetic Analyses ◽

Glandular Trichomes ◽

Anatomical Study ◽

Molecular Data ◽

Secretory Structures ◽

Phylogenetic Implications ◽

Secretory Cavities ◽

Morphological And Molecular Data ◽

Vegetative Characters

Abstract Phylogenetic analyses of early-diverging Faboideae have indicated that genera previously positioned in distinct tribes are instead closely related, e.g. in the Amburaneae clade, and the relatively recent rearrangements of many genera into clades has hampered the identification of morphological synapomorphies for previously unrecognized clades. Our aims are to evaluate anatomical vegetative characters of leaflets attached to reproductive features in the Amburaneae clade, to identify new synapomorphies for the clade and subclades, to identify characters supporting intergeneric relationships and diagnostic characters for the genus and species and to provide information about the morphology and histochemistry of secretory structures. The study was based on the anatomy of the leaflet of 19 species of the Amburaneae clade. Papillae cells, the presence of vascular system units and hypodermis are shared features of Amburaneae. Anatomical characters of the leaflet can be diagnostic at the generic and specific levels in Amburaneae. Secretory structures found in the clade are secretory cavities, secretory channels, idioblasts, mucilaginous epidermal cells and glandular trichomes. The broader concept of Amburaneae is reinforced here by morphological and molecular data, with the identification of new synapomorphies. Our dataset supports the intergeneric relationships resolved by molecular data.

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Chemical Composition of the Essential Oils from Two Vietnamese Asarum Species: A. glabrum and A. cordifolium

Natural Product Communications ◽

10.1177/1934578x1300800227 ◽

2013 ◽

Vol 8 (2) ◽

pp. 1934578X1300800 ◽

Cited By ~ 1

Author(s):

Tran Huy Thai ◽

Ophélie Bazzali ◽

Tran Minh Hoi ◽

Nguyen Anh Tuan ◽

Félix Tomi ◽

...

Keyword(s):

Chemical Composition ◽

Nmr Spectroscopy ◽

Endangered Species ◽

Essential Oil ◽

Essential Oils ◽

Spectroscopic Techniques ◽

Red Data Book ◽

Data Book ◽

First Time ◽

C Nmr

The essential oil isolated from Asarum cordifolium C. E. C. Fischer recently discovered in Vietnam, and A. glabrum Merr., an endangered species listed as vulnerable in the Red Data Book of Vietnam, have been analyzed by a combination of chromatographic and spectroscopic techniques including 13C NMR spectroscopy. The composition of A. cordifolium essential oil, investigated for the first time, was dominated by elemicin (82.5%). The essential oil isolated from A. glabrum contained safrole (41.9%) as its major component and was characterized by the diversity of phenylpropanoids contained in this oil (10 compounds).

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Anatomy of vegetative organs with an emphasis on the secretory structures of two species of Aldama (Asteraceae–Heliantheae)

Botany ◽

10.1139/cjb-2012-0271 ◽

2013 ◽

Vol 91 (6) ◽

pp. 335-342 ◽

Cited By ~ 19

Author(s):

Tuane Oliveira ◽

Aline Bertolosi Bombo ◽

Beatriz Appezzato-Da-Glória

Keyword(s):

Adventitious Roots ◽

Glandular Trichomes ◽

Diagnostic Value ◽

Aromatic Plants ◽

Secretory Structures ◽

Vegetative Organs ◽

Anatomical Features ◽

Anatomical Analysis ◽

Secretory Cavities ◽

Underground System

Aldama arenaria (Baker) E.E.Schill. & Panero and Aldama robusta (Gardner) E.E.Schill. & Panero are aromatic plants, morphologically very similar and usually confused on the analysis of exsiccates. The present study aimed to identify the secretory structures present in vegetative organs and to survey for anatomical features with diagnostic value between A. arenaria and A. robusta based on an examination of these organs. Aldama arenaria and A. robusta exhibit hydathodes in the dentate ornamentations of the leaf margins and apices in addition to glandular trichomes, secretory cavities and ducts, all of which vary with respect to type and location in each species. Xylopodia and adventitious roots constitute the underground system. The xylopodium is covered by epidermis or by suberised cells resulting from periclinal divisions of parenchyma cells. The tuberisation process of adventitious roots results mainly from hypertrophy and hyperplasia of medullary cells, especially in A. arenaria. Through anatomical analysis, useful features for distinction between A. arenaria and A. robusta in exsiccates and fresh materials were identified, and these features were related mainly to the position and frequency of secretory structures.

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Chemical Composition of the Essential Oils of Two Chinese Endemic Meconopsis Species

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-5-603 ◽

2003 ◽

Vol 58 (5-6) ◽

pp. 313-315 ◽

Cited By ~ 3

Author(s):

Changchun Yuan ◽

Peng Nan ◽

Suhua Shi ◽

Yang Zhong

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Hexadecanoic Acid ◽

First Time

Abstract The essential oils from two Chinese endemic Meconopsis species, i. e., M. punicea and M. delavayi, were analyzed by using GC-MS for the first time. The major constituents were hexadecanoic acid (16.8%), 1,2-dimethyl naphthalene (11.4%), 1,4-dimethyl naphthalene (6.6%), 1,3-dimethyl-5-ethyl naphthalene (5.9%), and 3-methyl biphenyl (5.6%) for M. punicea, and hexadecanoic acid (9.9%), 1,2-dimethyl naphthalene (7.9%), 1,3-dimethyl-5-ethyl naphthalene (6.2%), tetradecane (5.9%), and hexyl cinnamaldehyde (5.5%) for M. delavayi.

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Effect of the Distillation Time on the Chemical Composition, Antioxidant Potential and Antimicrobial Activity of Essential Oils from Different Cannabis sativa L. Cultivars

Molecules ◽

10.3390/molecules26164770 ◽

2021 ◽

Vol 26 (16) ◽

pp. 4770

Author(s):

Sara Palmieri ◽

Francesca Maggio ◽

Marika Pellegrini ◽

Antonella Ricci ◽

Annalisa Serio ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Cannabis Sativa ◽

Central Italy ◽

Antioxidant Potential ◽

Volatile Fraction ◽

Food Borne ◽

Spoilage Bacteria ◽

First Time

Within the unavoidable variability of various origins in the characteristics of essential oils, the aim of this study was to evaluate the effect of the distillation time on the chemical composition and biological activity of Cannabis sativa essential oils (EOs). The dry inflorescences came from Carmagnola, Kompolti, Futura 75, Gran Sasso Kush and Carmagnola Lemon varieties from Abruzzo region (Central Italy), the last two being new cultivar here described for the first time. EOs were collected at 2 h and 4 h of distillation; GC/MS technique was applied to characterize their volatile fraction. The EOs were evaluated for total polyphenol content (TPC), antioxidant capacity (AOC) and antimicrobial activity against food-borne pathogens and spoilage bacteria. The time of distillation particularly influenced EOs chemical composition, extracting more or less terpenic components, but generally enriching with minor sesquiterpenes and cannabidiol. A logical response in ratio of time was observed for antioxidant potential, being the essential oils at 4 h of distillation more active than those distilled for 2 h, and particularly Futura 75. Conversely, except for Futura 75, the effect of time on the antimicrobial activity was variable and requires further investigations; nevertheless, the inhibitory activity of all EOs against Pseudomonas fluorescens P34 was an interesting result.

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Taxonomic traits in the microstructure of flowers of parasitic Orobanche picridis with particular emphasis on secretory structures

PROTOPLASMA ◽

10.1007/s00709-019-01438-3 ◽

2019 ◽

Vol 257 (1) ◽

pp. 299-317 ◽

Cited By ~ 1

Author(s):

Agata Konarska ◽

Piotr Chmielewski

Keyword(s):

Neutral Lipids ◽

Phenotypic Variability ◽

Glandular Trichomes ◽

Starch Granules ◽

Secretory Structures ◽

Transmission Electron ◽

Root Parasite ◽

Bright Field Microscopy ◽

High Level ◽

First Time

Abstract Orobanche picridis is an obligate root parasite devoid of chlorophyll in aboveground organs, which infects various Picris species. Given the high level of phenotypic variability of the species, the considerable limitation of the number of taxonomically relevant traits (mainly in terms of generative elements), and the low morphological variation between species, Orobanche is regarded as one of the taxonomically most problematic genera. This study aimed to analyse the taxonomic traits of O. picridis flowers with the use of stereoscopic and bright-field microscopy as well as fluorescence, scanning, and transmission electron microscopy. The micromorphology of sepals, petals, stamens, and pistils was described. For the first time, the anatomy of parasitic Orobanche nectaries and the ultrastructure of nectaries and glandular trichomes were presented. Special attention was paid to the distribution and types of glandular and non-glandular trichomes as well as the types of metabolites contained in these structures. It was demonstrated that the nectary gland was located at the base of the gynoecium and nectar was secreted through modified nectarostomata. The secretory parenchyma cells contained nuclei, large amyloplasts with starch granules, mitochondria, and high content of endoplasmic reticulum profiles. Nectar was transported via symplastic and apoplastic routes. The results of histochemical assays and fluorescence tests revealed the presence of four groups of metabolites, i.e. polyphenols (tannins, flavonoids), lipids (acidic and neutral lipids, essential oil, sesquiterpenes, steroids), polysaccharides (acidic and neutral polysaccharides), and alkaloids, in the trichomes located on perianth elements and stamens.

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Composition of Seed Essential Oils of Rhododendron tomentosum

Natural Product Communications ◽

10.1177/1934578x1200700229 ◽

2012 ◽

Vol 7 (2) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Asta Judzentiene ◽

Rita Butkiene ◽

Jurga Budiene ◽

Félix Tomi ◽

Joseph Casanova

Keyword(s):

Quantitative Analysis ◽

Chemical Composition ◽

Nmr Spectroscopy ◽

Essential Oil ◽

Essential Oils ◽

Thermal Isomerization ◽

Total Oil ◽

First Time ◽

Rhododendron Tomentosum ◽

C Nmr

For the first time, the chemical composition of the seed essential oil of Rhododendron tomentosum was determined. Forty-seven compounds were identified, comprising 91.7% of the total oil. Palustrol (38.3%) and ledol (27.0%) were the predominant constituents. Some constituents, such as β-pinene oxide, iso-menthyl acetate, nerolidyl acetate, cadalene and guaiazulene were characteristic only for the seeds and were identified for the first time in Rh. tomentosum oils. For comparison purposes, the essential oil isolated from the shoots of the same plant were analyzed [GC(FID) in combination with RIs, GC-MS and 13C NMR]. More than a half of the oil was comprised of ledol (36.5%) and palustrol (21.0%). Quantitative analysis of ascaridol, a heat-sensitive compound, was carried out by 13C NMR spectroscopy. Indeed, ascaridol undergoes partial thermal isomerization to iso-ascaridol during GC analyses.

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