A duplex ddPCR assay for simultaneously detecting Ips sexdentatus and Ips typographus (Coleoptera: Curculionidae) in bulk trap samples

2019 ◽  
Vol 49 (8) ◽  
pp. 903-914 ◽  
Author(s):  
Frida A. Zink ◽  
Luke R. Tembrock ◽  
Alicia E. Timm ◽  
Todd M. Gilligan
Keyword(s):  
Dna Extraction ◽  
Bark Beetles ◽  
Digital Pcr ◽  
The United States ◽  
Ips Typographus ◽  
Morphological Identification ◽  
Efficient Manner ◽  
Large Numbers ◽  
The Family ◽  
Ips Sexdentatus

Bark beetles in the family Curculionidae present a growing hazard to forests worldwide. Like native bark beetles, introduced exotic species can pose a serious threat to North American forests. Ips typographus (Boerner) and Ips sexdentatus (Linnaeus), both native to Europe, are two such pests that have caused widespread forest loss in their native ranges. International trade has led to increased interceptions of Scolytine beetles at ports of entry to the United States. Most intercepted individuals are not identified to species due to lack of expert identifiers, poor specimen quality, or incomplete taxonomy. These same problems affect identification for domestic surveys. Therefore, development of molecular methods for identification of potentially invasive Ips species is essential. Because of the need to scrutinize large numbers of beetles in an efficient manner, we describe a duplex droplet digital PCR (ddPCR) assay to identify I. typographus and I. sexdentatus simultaneously in bulk trap samples containing 500 Scolytinae specimens using a scalable, two-step DNA extraction. This ddPCR method is highly effective for processing the entire contents of beetle traps and identifying these potentially invasive species in a timely and definitive manner. We also describe a nondestructive DNA extraction technique that preserves specimens for morphological identification.

Reuniting children with their families during disasters: A proposed plan for greater success

2007 ◽  
Vol 2 (3) ◽  
pp. 113-117 ◽  
Author(s):  
Sarita Chung, MD ◽  
Michael Shannon, MD, MPH
Keyword(s):  
Emergency Preparedness ◽  
Terrorist Attack ◽  
Unmet Need ◽  
The United States ◽  
Pediatric Emergency ◽  
Patient Privacy ◽  
Extraction Technology ◽  
Eye Color ◽  
Large Numbers ◽  
The Family

In the event of a terrorist attack or natural disaster, large numbers of children may be separated from their families and caregivers. Many of these children will present for treatment at emergency departments or be evacuated to relocation sites. Depending on their age, some children may not be able to give their name or may be too frightened to give any information, making identification difficult. At the same time, parents will instinctively rush to hospitals to find their children. In the process, parents may unintentionally obstruct medical care, overwhelm an already stressed staff, and violate patient privacy as they frantically search for their children. Currently, there is no system in the United States that effectively expedites the reunification of children with their families when children can not be identified by healthcare or public health personnel.We propose the creation of a system that employs advanced imaging and feature-extraction technology. We envision a system in which digital images of individual children are captured as they enter a facility, with these images then being automatically transmitted and posted on a secure Web site. Features of each image, such as hair and eye color, would be automatically indexed and cataloged. With the help of trained professionals, parents could enter their child’s features into the system and receive a limited set of images for identification, allowing for rapid reunification of the family. Additional advanced features of such a system will be explored. If successful, the use of such a system would address an important unmet need in pediatric emergency preparedness.

scholarly journals Efficacy of synthetic lures for pine bark beetle monitoring

2022 ◽  
Vol 68 (No. 1) ◽  
pp. 19-25
Author(s):  
Miloš Knížek ◽  
Jan Liška ◽  
Adam Véle
Keyword(s):  
Bark Beetle ◽  
Bark Beetles ◽  
Large Scale ◽  
Ips Typographus ◽  
Pine Plantations ◽  
The Czech Republic ◽  
Long Run ◽  
Baited Traps ◽  
Pine Trees ◽  
Ips Sexdentatus

The Scots pine (Pinus sylvestris) plantations in central Europe are currently damaged by a large-scale infestation by bark beetles (Scolytinae). Ips acuminatus and Ips sexdentatus are among the most aggressive species causing infestations of pine trees that are currently simultaneously attacked by Ips typographus. In pine plantations prone to damage, it is therefore necessary to carry out the bark beetle monitoring. One of the used methods is the pheromone bark beetle trapping using synthetic lures. The efficacy of synthetic lures differs. We tested the efficacy of commercially available lures used in the protection of pine trees. In total, we deployed 10 trap series, each consisting of traps with eight different lures and two unbaited traps (controls). Ips acuminatus and I. sexdentatus were most abundantly captured in Pheagr-IAC- and Sexowit-baited traps. Interestingly, the spruce species I. typographus was also captured and most often found in traps with Pheagr-IAC and Erosowit Tube lures. The number of captured beetles was consistent with the gradation phase of bark beetles. Our results suggest the suitability of pheromone traps for bark beetle monitoring. The use of Sexowit can be recommended especially in southwestern Moravia, where I. sexdentatus occurs in high numbers in the long run. In other parts of the Czech Republic, Pheagr-IAC alone can be used with sufficient efficacy. The use of the Erosowit Tube lure is also suitable for I. typographus and I. sexdentatus monitoring.

scholarly journals Naturally-occurring entomopathogenic fungi on three bark beetle species (Coleoptera: Curculionidae) in Bulgaria

2010 ◽  
Vol 25 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Slavimira Draganova ◽  
Danail Takov ◽  
Danail Doychev
Keyword(s):  
Entomopathogenic Fungi ◽  
Bark Beetles ◽  
Fungal Pathogens ◽  
Morphological Characteristics ◽  
Ips Typographus ◽  
Beauveria Brongniartii ◽  
Naturally Occurring ◽  
Fungal Entomopathogens ◽  
Pure Cultures ◽  
Ips Sexdentatus

Bark beetles (Coleoptera: Curculionidae: Scolytinae) belong to one of the most damaging groups of forest insects and the activity of their natural enemies - pathogens, parasitoids, parasites or predators suppressing their population density, is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species - Ips sexdentatus Boern, Ips typographus (L.) and Dryocoetes autographus (Ratz.). Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collected from forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract) and were identified based on morphological characteristics both on the host and in a culture. Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. - Criv.) Vuillemin, Beauveria brongniartii (Saccardo) Petch and Isaria farinosa (Holmsk.) Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae). Morphological traits of the isolates are described.

Breaking Free

2017 ◽  
Author(s):  
Deirdre David
Keyword(s):  
United States ◽  
The United States ◽  
The Novel ◽  
Male Homosexuality ◽  
Domestic Life ◽  
The Family

In the mid- to late 1950s, Pamela emerged as a critically acclaimed novelist, particularly after the family returned to London. In perhaps her best-known novel, The Unspeakable Skipton, she explores the life of a paranoid writer who sponges on English visitors to Bruges. The novel was hailed for its wit and sensitive depiction of the life of a writer. She also published a fine study of a London vicar martyred in marriage to a vain and selfish wife: The Humbler Creation is remarkable for its incisive and empathetic depiction of male despair. The Last Resort sealed her distinction as a brilliant novelist of domestic life in its frank depiction of male homosexuality. While continuing to publish fiction, Pamela maintained her reputation as a deft reviewer. In 1954, she and Charles travelled to the United States—the first of many trips that were to follow.

The Constitutionality of Involuntary Civil Commitment of Opiate Addicts

1988 ◽  
Vol 18 (4) ◽  
pp. 641-661 ◽  
Author(s):  
Michael P. Rosenthal
Keyword(s):  
New York ◽  
Supreme Court ◽  
Civil Commitment ◽  
The United States ◽  
Involuntary Treatment ◽  
Large Numbers ◽  
Narcotic Trafficking ◽  
The Supreme Court ◽  
Opiate Addicts ◽  
The 1960S

This paper deals with the constitutionality of involuntary treatment of opiate addicts. Although the first laws permitting involuntary treatment of opiate addicts were enacted in the second half of the nineteenth century, addicts were not committed in large numbers until California and New York enacted new civil commitment legislation in the 1960s. Inevitably, the courts were called upon to decide if involuntary treatment was constitutional. Both the California and New York courts decided that it was. These decisions were heavily influenced by statements made by the United States Supreme Court in Robinson v. California. The Robinson case did not actually involve the constitutionality of involuntary treatment; it involved the question of whether it was constitutional for a state to make addiction a crime. Nevertheless, the Supreme Court declared (in a dictum) that a state might establish a program of compulsory treatment for opiate addicts either to discourage violation of its criminal laws against narcotic trafficking or to safeguard the general health or welfare of its inhabitants. Presumably because the Robinson case did not involve the constitutionality of involuntary treatment of opiate addicts, the Supreme Court did not go into that question as deeply as it might have. The California and New York courts, in turn, relied too much on this dictum and did not delve deeply into the question. The New York courts did a better job than the California courts, but their work too was not as good as it should have been.

scholarly journals Drivers of Spruce Bark Beetle (Ips typographus) Infestations on Downed Trees after Severe Windthrow

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1290
Author(s):  
Branislav Hroššo ◽  
Pavel Mezei ◽  
Mária Potterf ◽  
Andrej Majdák ◽  
Miroslav Blaženec ◽  
...  
Keyword(s):  
Solar Radiation ◽  
Bark Beetle ◽  
Bark Beetles ◽  
Ips Typographus ◽  
Wind Damage ◽  
Stem Length ◽  
Local Conditions ◽  
Spruce Bark Beetle ◽  
Management Actions ◽  
Spruce Bark

Research Highlights: Bark beetles are important agents of disturbance regimes in temperate forests, and specifically in a connected wind-bark beetle disturbance system. Large-scale windthrows trigger population growth of the European spruce bark beetle (Ips typographus L.) from endemic to epidemic levels, thereby allowing the killing of Norway spruce trees over several consecutive years. Background and Objectives: There is a lack of evidence to differentiate how outbreaks are promoted by the effects of environmental variables versus beetle preferences of trees from endemic to outbreak. However, little is known about how individual downed-tree characteristics and local conditions such as tree orientation and solar radiation affect beetle colonization of downed trees. Materials and Methods: To answer this question, we investigated the infestation rates and determined tree death categories (uprooted, broken, and stump) in wind-damaged areas in Western Tatra Mts. in Carpathians (Slovakia) from 2014–2016, following a windthrow in May 2014. In total, we investigated 225 trees over eight transects. For every tree, we measured its morphological (tree height, crown characteristics), environmental (solar radiation, terrain conditions, trunk zenith), temporal (time since wind damage), and beetle infestation (presence, location of attack, bark desiccation) parameters. We applied Generalized Additive Mixed Models (GAMM) to unravel the main drivers of I. typographus infestations. Results: Over the first year, beetles preferred to attack broken trees and sun-exposed trunk sides over uprooted trees; the infestation on shaded sides started in the second year along with the infestation of uprooted trees with lower desiccation rates. We found that time since wind damage, stem length, and incident solar radiation increased the probability of beetle infestation, although both solar radiation and trunk zenith exhibited nonlinear variability. Our novel variable trunk zenith appeared to be an important predictor of bark beetle infestation probability. We conclude that trunk zenith as a simple measure defining the position of downed trees over the terrain can anticipate beetle infestation. Conclusions: Our findings contribute to understanding of the bark beetle’s preferences to colonize windthrown trees in the initial years after the primary wind damage. Further, our findings can help to identify trees that are most susceptible to beetle infestation and to prioritize management actions to control beetle population while maintaining biodiversity.

Self-Efficacy Regarding Social Work Competencies

2015 ◽  
Vol 27 (5) ◽  
pp. 594-606 ◽  
Author(s):  
Gary Holden ◽  
Kathleen Barker ◽  
Sofie Kuppens ◽  
Gary Rosenberg
Keyword(s):  
Social Work ◽  
Educational Outcomes ◽  
Self Efficacy ◽  
Empirical Work ◽  
Preliminary Evidence ◽  
Efficient Manner ◽  
Replication Studies ◽  
Large Numbers ◽  
Social Work Programs ◽  
Work Education

Purpose: The need for psychometrically sound measurement approaches to social work educational outcomes assessment is increasing. Method: The research reported here describes an original and two replication studies of a new scale ( N = 550) designed to assess an individual’s self-efficacy regarding social work competencies specified by the Council on Social Work Education as part of the accreditation of social work programs. Results: This new measure, the Self-Efficacy Regarding Social Work Competencies Scale (SERSWCS), generally performed in line with our expectations. Discussion: The SERSWCS is a measure that is based on substantial theoretical and empirical work, has preliminary evidence regarding the psychometric properties of the data it produces, can be used with large numbers of students in an efficient manner, is neither expensive or subject to user restrictions, and provides views of outcomes that have utility for pedagogical considerations at multiple curricular levels.

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