Study of Red Halophilic Bacteria in Solar Salt and Salted Fish: I. Effect of Bacto-Oxgall

1956 ◽  
Vol 13 (2) ◽  
pp. 183-194 ◽  
Author(s):  
H. P. Dussault
Keyword(s):  
Halophilic Bacteria ◽  
Sodium Taurocholate ◽  
Selective Medium ◽  
Microscopical Examination ◽  
Solar Salt ◽  
Salted Fish ◽  
Mechanical Disruption ◽  
Inhibitory Power ◽  
Bacterial Cytoplasm ◽  
Ph Variations

Bacto-oxgall has shown a differential effect on the red halophilic bacteria commonly found in solar salt and in contaminated salted fish. Pseudomonas salinaria was inhibited by low oxgall concentrations while Sarcina littoralis tolerated high ones. When this differentiating test is applied to a larger number of strains it will be possible to determine if Bacto-oxgall can be used as the basis of a selective medium to distinguish one genus from the other. The clearing of bacterial suspensions and microscopical examination have shown that the inhibition of Ps. salinaria was produced by the lysis of the cells. The inhibition of Ps. salinaria was effective and rapid. It was not affected by pH variations but was reduced by the presence of proteins. When compared similarly to other bile products, Bacto-oxgall was found to have the same inhibitory power as sodium taurocholate. Bacto-oxgall, diluted to the equivalent of fresh bile, was also found to be three times more effective than fresh cod bile. From the evidence gathered it was concluded that the inhibition of Ps. salinaria by Bacto-oxgall is due to a mechanical disruption of the bacterial cytoplasm.

Study of Red Halophilic Bacteria in Solar Salt and Salted Fish: II. Bacto-oxgall as a Selective Agent for Differentiation

1956 ◽  
Vol 13 (2) ◽  
pp. 195-199 ◽  
Author(s):  
H. P. Dussault
Keyword(s):  
Halophilic Bacteria ◽  
Partial Identification ◽  
Selective Medium ◽  
Selective Agent ◽  
Simple Method ◽  
Solar Salt ◽  
Salted Fish ◽  
Low Concentrations ◽  
Fish Samples

A simple method, based on oxgall tolerance, is proposed for differentiating red halophilic bacteria commonly found in solar salt and discoloured salted codfish. Tests carried out on 18 strains isolated from various sources have shown that the rod forms are inhibited by low concentrations of Bacto-oxgall and that the coccus forms tolerate relatively high ones. Bacto-oxgall can thus be used as the basis of a simple selective medium. This test has been found useful for the isolation, purification and partial identification of unidentified strains and also for determining the relative proportions of the two main types of red halophilic bacteria present in solar salt and salted fish samples.

Population of halophilic bacteria in salted fish products made in the Loochoo Islands, Okinawa and the Noto Peninsula, Ishikawa, Japan

2002 ◽  
Vol 68 (6) ◽  
pp. 1265-1273 ◽  
Author(s):  
TAKASHI KUDA ◽  
KAORI OKAMOTO ◽  
TOSHIHIRO YANO
Keyword(s):  
Halophilic Bacteria ◽  
Fish Products ◽  
Salted Fish ◽  
Made In

scholarly journals In vitro Antibacterial Potential of Halophilic Bacterial Secondary Metabolites from Salted Fish

2016 ◽  
Vol 8 (04) ◽  
Author(s):  
G. Prakash Williams ◽  
Anju Anand ◽  
Parvathy A. ◽  
Rakky C. Raj ◽  
Robert Raju ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Xanthomonas Campestris ◽  
Halophilic Bacteria ◽  
Resistance Pattern ◽  
Salted Fish ◽  
Wide Range ◽  
Fish Samples

Living organisms can be found over a wide range of extreme conditions. Most of the organisms living in extreme environments (i.e, extremophiles) belong to the prokaryotes. Halophiles are interesting class of extremophilic organisms that have adapted to harsh, hypersaline conditions. They are able to compete successfully for water and resist the denaturing effects of salts. The present study was an investigation on the in vitro antibacterial effect of secondary metabolites from halophilic bacteria isolated from salted fish samples. The cured salted fish samples were collected and enumerated using halophilic Nutrient Agar supplemented with 4% NaCl. The isolated and purified bacterial cultures are numbered as SF1, SF2, SF3, SF4 and SF5 are further identified using VITEK 2 system as Bacillus vallismortis, Ralstonia mannitolytica, Bacillus subtilis, Rhizoboum radiobacter and Kocuria kristina. Growth kinetics of halobacterial isolates were determined by spectrophotometric assay. The antibiotic resistance pattern of tested pathogenic microorganisms using the commercial antibiotics was screened and almost all the tested microorganisms are resistant to Penicillin. The antimicrobial activity of secondary metabolites of halophilic bacteria against drug resistant microbes was assessed using the Agar well diffusion assay. Among the different extracts of the halophilic bacteria, the chloroform extracts of R. mannitolytica showed maximum antibacterial activity against Bacillus subtilis MTCC 441 and Xanthomonas campestris MTCC 2286. The results of antimicrobial activity are considerable because it enables the identification of potential secondary metabolites present in marine halophilic bacteria, which act as source of innumerable therapeutic agents. Further research is highly warranted to find out the active principle responsible for the antibacterial property and to elucidate the structure of particular compound.

scholarly journals Perchlorate-Reducing Bacteria from Hypersaline Soils of the Colombian Caribbean

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Rosa Acevedo-Barrios ◽  
Angela Bertel-Sevilla ◽  
Jose Alonso-Molina ◽  
Jesus Olivero-Verbel
Keyword(s):  
Halophilic Bacteria ◽  
Industrial Applications ◽  
Rrna Gene ◽  
Environmental Matrices ◽  
Bacterial Strains ◽  
Gene Sequence Analysis ◽  
Degrading Bacteria ◽  
Polluted Sites ◽  
Reducing Bacteria ◽  
Ph Variations

Perchlorate (ClO4−) has several industrial applications and is frequently detected in environmental matrices at relevant concentrations to human health. Currently, perchlorate-degrading bacteria are promising strategies for bioremediation in polluted sites. The aim of this study was to isolate and characterize halophilic bacteria with the potential for perchlorate reduction. Ten bacterial strains were isolated from soils of Galerazamba-Bolivar, Manaure-Guajira, and Salamanca Island-Magdalena, Colombia. Isolates grew at concentrations up to 30% sodium chloride. The isolates tolerated pH variations ranging from 6.5 to 12.0 and perchlorate concentrations up to 10000 mg/L. Perchlorate was degraded by these bacteria on percentages between 25 and 10. 16S rRNA gene sequence analysis indicated that the strains were phylogenetically related toVibrio,Bacillus,Salinovibrio,Staphylococcus, andNesiotobactergenera. In conclusion, halophilic-isolated bacteria from hypersaline soils of the Colombian Caribbean are promising resources for the bioremediation of perchlorate contamination.

Studies on the red halophilic bacteria from salted fish and salt

1954 ◽  
Vol 39 (1) ◽  
pp. 17-23 ◽  
Author(s):  
R. Venkataraman ◽  
A. Sreenivasan
Keyword(s):  
Halophilic Bacteria ◽  
Salted Fish

Further studies on the red halophilic bacteria from solar salts and salted fish

1956 ◽  
Vol 43 (3) ◽  
pp. 197-206
Author(s):  
R. Venkataraman ◽  
A. Sreenivasan
Keyword(s):  
Halophilic Bacteria ◽  
Salted Fish

scholarly journals Temporal and salinity impacts on the microbial diversity at the Eilat, Israel Solar Salt Plant

2013 ◽  
Vol 11 (1) ◽  
pp. 86-90 ◽  
Keyword(s):  
Microbial Community ◽  
Halophilic Bacteria ◽  
Halophilic Archaea ◽  
Extensive Study ◽  
Molecular Techniques ◽  
Molecular Fingerprinting ◽  
Solar Salt ◽  
Salt Works ◽  
Pure Cultures ◽  
Plate Counts

A major solar salt works in Israel is located just north of the town of Eilat and close to the airport. This solar salt works has been in operation since 1977 and produces about 170,000 tons a year. A three-year study was initiated to examine the temporal and salinity impacts on the microbial community throughout this saltern. At the time this study was begun in the late 1990's, there were several paradigms about the microbial community: 1. there is greatly reduced diversity as the water reaches saturation; 2. because of the relative stability of the salt concentrations in the various pans, there is little change in the microbial community; and, 3. halophilic Archaea are not found in ponds with less than 15% salt and halophilic Bacteria would not be found in pans at saturation. The first paradigm was developed based on a few studies using one medium and single observations of various salterns. The second paradigm was an assumption that had not been systematically tested, and the third paradigm, at least for the Bacteria, was shown in 1980 not to be true with the discovery of the genus Halomonas. We examined these paradigms by systematically studying on a seasonal basis the saltern at Eilat, Israel, as well as the Cargill Solar Salt Plant in Newark, California. Both traditional and molecular techniques have been used throughout these studies. Several different approaches were taken: plate counts were made on several different media, whole community carbon requirements were determined, molecular fingerprinting of the whole microbial community using the amplicon length heterogeneity approach was performed to determine the changes in the community composition over time, and partial characterization of the isolated pure cultures were all performed. Samples were taken at least twice a year corresponding to the cooler and hotter times of the year. Based on this extensive study there were significant differences in the plate counts and in the ratios of the various peaks found during the fingerprinting. It is safe to say now that the microbial community in the waters of a solar saltern is variable and both domains can be found throughout the saltworks.

scholarly journals Application of halophilic bacteria in traditional solar salt pond: a preliminary study

2019 ◽  
Vol 404 ◽  
pp. 012035
Author(s):  
E Chasanah ◽  
A Pratitis ◽  
D Ambarwati ◽  
D Fithriani ◽  
R Susilowati ◽  
...  
Keyword(s):  
Halophilic Bacteria ◽  
Solar Salt ◽  
Salt Pond ◽  
Preliminary Study

scholarly journals Kocuria marina BS-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India

2014 ◽  
Vol 21 (6) ◽  
pp. 511-519 ◽  
Author(s):  
Yesurethinam Sarafin ◽  
Mariathasan Birdilla Selva Donio ◽  
Subramanian Velmurugan ◽  
Mariavincent Michaelbabu ◽  
Thavasimuthu Citarasu
Keyword(s):  
Halophilic Bacteria ◽  
Solar Salt ◽  
Salt Works
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