Histopathological and Hematological Changes in Mummichogs, Fundulus heteroclitus, Infected by a Pseudomonad

1978 ◽  
Vol 35 (10) ◽  
pp. 1376-1381 ◽  
Author(s):  
M. F. Li ◽  
G. S. Traxler ◽  
S. Clyburne
Keyword(s):  
Fundulus Heteroclitus ◽  
Renal Tissue ◽  
Infected Fish ◽  
Splenic Tissue ◽  
Hepatic Veins ◽  
Renal Tubules ◽  
Fish Disease ◽  
Histopathological Changes ◽  
Key Words Fish ◽  
Hematological Changes

The hematological and histopathological changes in mummichogs infected by Pseudomonas reptilivora included a dramatic reduction in hematocrit values; massive structural lymphoid necrosis with formation of edematous spaces in the splenic tissue; empty and degenerate hepatic veins and sinusoids; and necrosis of the renal tubules in naturally and experimentally infected fish. Electron microscopy of the renal tissue revealed clumps of chromatin in the nuclei, disintegration or degeneration of organelles, and an increase of digestive vesicles or autophagic vacuoles in the cells. This is the first description of the pathology of this bacteria in a marine fish. Key words: fish disease, Pseudomonas, bacteria, hemorrhagic septicemia

​Safety Evaluation of Andrographolide-nanosuspensions

2021 ◽  
Author(s):  
Haigang Wu ◽  
Jia Liu ◽  
Zhaowei Ye ◽  
Jinni Liu ◽  
Li Huang ◽  
...  
Keyword(s):  
Toxicity Test ◽  
Dose Group ◽  
In Vitro Release ◽  
Liver Cells ◽  
Precipitation Method ◽  
Hepatic Veins ◽  
High Dose ◽  
Interstitial Tissue ◽  
Renal Tubules ◽  
Cell Counts

Background: Andrographolide (ANDRO) is a hydrophobic drug, which faces the problem of limited absorption due to poor water solubility. The current research prepared andrographolide nanosuspensions (ANDRO-NS) and examined in vivo toxicity for mice. Methods: ANDRO-NS were prepared by anti-solvent precipitation method, transmission electron micrographs, granularity analysis and in vitro release were used to characterize the ANDRO-NS, we evaluate the safety of the ANDRO-NS by using the acute toxicity test, local irritation test and chronic toxicity test. Result: The particle size of ANDRO-NS was (568.51±13.74 nm). The LD50 for ANDRO-NS was 548.91 mg/kg after oral administration to KM mice with a 95% CI of 468.19-645.03 mg/kg. The white blood cell counts and hemoglobin levels for the experimental groups were lower than controls receiving only saline. Serum aspartate transaminase, creatinine and blood urea nitrogen levels were greater than controls after 7 and 14 days of once-daily administration. After 14 days of administration, the platelet counts as well asalanine transaminase levels were, in addition, Histological observations indicated that interstitial kidney tissues were wider than controls and showed episodic bleeding after 7 days of administration. The highest dose administered also resulted in the dilation and blood engorgement of the central hepatic veins with some severed hepatic cords. Mice receiving the lowest dose of ANDRO-NS we administered appeared healthy and similar to controls receiving saline only. Following 14 days of administration, we found significant vacuolar degeneration of renal tubular epithelial cells and glomerular atrophy for the high-dose group as well as edema and necrosis in liver cells. The medium-dose group displayed kidney interstitial tissue widening with scattered bleeding, inflammatory cell infiltration and hepatocyte edema. The low-dose group displayed dilated renal tubules and irregularly-arranged liver cells as well as bleeding in the hepatic sinusoids. Therefore, short-term administration of andrographolide suspensions resulted in inflammation and time- and dose-dependent toxic effects on the kidneys and liver.

scholarly journals Cardiovascular and hematologic effects produced by chronic treatment with etoricoxib in normotensive rats

2009 ◽  
Vol 24 (3) ◽  
pp. 206-210 ◽  
Author(s):  
Nilo César do Vale Baracho ◽  
Guilherme Pedrosa Guizelli ◽  
Beatriz Leone Carmello ◽  
Danielle de Souza Sanches ◽  
Felipe Moraes Costa Silva ◽  
...  
Keyword(s):  
Blood Cells ◽  
Chronic Treatment ◽  
Experimental Period ◽  
Control Group ◽  
Histopathological Changes ◽  
Study Results ◽  
Significant Difference ◽  
Hematological Effects ◽  
The Mean ◽  
Hematological Changes

PURPOSE: Evaluate the cardiovascular and hematological effects produced by chronic treatment with two dosis of etoricoxib in Wistar normotensive rats. METHODS: Thirty rats have been used and divided into one control group and two etoricoxib (10mg/kg and 30mg/kg) treatments groups for 60 days. The mean arterial pressure (MAP) was taken during the whole experimental period and at the end of this period, under anesthesia blood samples were taken, and further the withdrawn of the aorta, heart, brain, liver, and kidneys for the anatomopathologic study. RESULTS: The treatment with etoricoxib (30mg/Kg) produced a significant increase of the MAP from the 28th day of the experiment and from the platelets when compared to the control group and to the group treated with 10mg/Kg, besides producing a highly significant difference in hematocrit and in the red blood cells in relation to the control group. On the other hand the treatment with etoricoxib has not caused histopathological changes when compared to the control. CONCLUSION: These data show that the chronic treatment with etoricoxib leads to increase of the MAP, and to important hematological changes which seem to be associated to the hemoconcentration although not producing anatomopathological significant changes.

Secretory NaCl and volume flow in renal tubules

1986 ◽  
Vol 250 (5) ◽  
pp. R753-R763 ◽  
Author(s):  
K. W. Beyenbach
Keyword(s):  
Fluid Secretion ◽  
Renal Tissue ◽  
Tubular Secretion ◽  
Proximal Tubules ◽  
Epithelial Cell Line ◽  
Renal Tubules ◽  
Genetic Potential ◽  
Dog Kidney ◽  
Nacl Secretion

This review attempts to give a retrospective survey of the available evidence concerning the secretion of NaCl and fluid in renal tubules of the vertebrate kidney. In the absence of glomerular filtration, epithelial secretory mechanisms, which to this date have not been elucidated, are responsible for the renal excretion of NaCl and water in aglomerular fish. However, proximal tubules isolated from glomerular fish kidneys of the flounder, killifish, and the shark also have the capacity to secrete NaCl and fluid. In shark proximal tubules, fluid secretion appears to be driven via secondary active transport of Cl. In another marine vertebrate, the sea snake, secretion of Na (presumably NaCl) and fluid is observed in freshwater-adapted and water-loaded animals. Proximal tubules of mammals can be made to secrete NaCl in vitro together with secretion of aryl acids. An epithelial cell line derived from dog kidney exhibits secondary active secretion of Cl when stimulated with catecholamines. Tubular secretion of NaCl and fluid may serve a variety of renal functions, all of which are considered here. The occurrence of NaCl and fluid secretion in glomerular proximal tubules of teleosts, elasmobranchs, and reptiles and in mammalian renal tissue cultures suggests that the genetic potential for NaCl secretion is present in every vertebrate kidney.

scholarly journals Isolation and pathological study of Branchiomycosis from the commercial pond of common carp (Cyprinus carpio) fish, in Governorate of Duhok / Iraq

2011 ◽  
Vol 35 (1) ◽  
pp. 1-9
Author(s):  
Khalid Subhi Ibrahim
Keyword(s):  
Cyprinus Carpio ◽  
Water Surface ◽  
Infected Fish ◽  
Histopathological Changes ◽  
Pathological Study ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Causal Pathogen ◽  
Carp Fish ◽  
Cyprinus Carpio Fish

Branchiomycosis is a fungal disease that infects fish gills. It wasidentified by isolation and histopathological changes of examined gills incommon carp fish (Cyprinus carpio) which, were obtained from fish farm inDuhok Governorate, Iraq. The infected fish were suffering from respiratorydisorders; gulping air at the water surface, rapid movement of operculum andmassive mortality, which resulted in the loss of 95% of fish pond. The gillsappear marbled appearance with necrotic areas on the localized damage gills.The causal pathogen was identified as Branchiomyces sanguinis, in which thediameter of spores and non-septated hyphae are 5-7 μm and 12 – 20 μm,respectively. In histopathological preparation, the spores and the non-septatedhyphae have been shown to be embedded in the gill tissues contained undividedand sporulating stages.

scholarly journals Ameliorative Effects of Ascorbic Acid and Allium sativum (Garlic) Ethanol Extract on Renal Parenchyma of Gentamicin-induced Nephropathic Rats

2020 ◽  
pp. 1-8
Author(s):  
Dayo Rotimi Omotoso ◽  
Joy Motunrayo Olajumoke
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Allium Sativum ◽  
Combined Treatment ◽  
Ethanol Extract ◽  
Renal Tissue ◽  
Renal Parenchyma ◽  
Histopathological Changes ◽  
Treatment Groups ◽  
Tissue Weight

To assess ameliorative effects of Ascorbic acid (AA) and Allium sativum ethanol extract (ASEE) on renal parenchyma of gentamicin-induced nephropathic rats. Thirty Wistar rats (weighing between 180-205 g) were randomly divided into five groups (A-E).  These include Group A administered with 0.9% Normal Saline (0.5 ml/kg body weight (b.w.)), Group B administered with gentamicin (GM, 200 mg/kg b.w.) intraperitoneally (i.p.), Group C administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally, Group D administered with GM (200 mg/kg b.w.) i.p. and ASEE (200 mg/kg b.w.) orally and Group E administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally and ASEE (200 mg/kg b.w.) orally. All administrations were done once daily for a period of ten (10) days. The body weight of study animals was recorded at the beginning and end of study period. After the study period, renal tissue of study animals was harvested, weighed, processed, stained using H & E technique. Stained sections were examined under microscope for histopathological changes within the renal parenchyma and were scored using image-J software. The results of this study showed that exposure to GM results into significant (P < 0.05) reduction in body and renal tissue weight. However, therapeutic exposure to AA and ASEE either as individual or combined treatment regimen culminated into relatively null body and renal tissue weight loss among treatment groups C-E. In addition, exposure to GM precipitates prominent histopathological changes within renal parenchyma of study animals.  As observed with body and renal tissue weight changes, treatment with AA and ASEE also comparatively ameliorate GM-induced nephropathy within renal parenchyma of study animals in treatment groups. The findings of this study therefore showed that AA and ASEE exhibit ameliorative effect on the renal parenchyma of gentamicin-induced nephropathic rats either as distinct or combined treatment regimen.

Transport of a fluorescent cAMP analog in teleost proximal tubules

2007 ◽  
Vol 293 (6) ◽  
pp. R2382-R2389 ◽  
Author(s):  
Valeska Reichel ◽  
Rosalinde Masereeuw ◽  
Jeroen J. M. W. van den Heuvel ◽  
David S. Miller ◽  
Gert Fricker
Keyword(s):  
Fundulus Heteroclitus ◽  
Organic Anion ◽  
Membrane Vesicles ◽  
Proximal Tubules ◽  
Organic Anion Transporter ◽  
Renal Tubules ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Camp Analog ◽  
Anion Transporter

Previous studies have shown that killifish ( Fundulus heteroclitus) renal proximal tubules express a luminal membrane transporter that is functionally and immunologically analogous to the mammalian multidrug resistance-associated protein isoform 2 (Mrp2, ABCC2). Here we used confocal microscopy to investigate in killifish tubules the transport of a fluorescent cAMP analog (fluo-cAMP), a putative substrate for Mrp2 and Mrp4 (ABCC4). Steady-state luminal accumulation of fluo-cAMP was concentrative, specific, and metabolism-dependent, but not reduced by high K+ medium or ouabain. Transport was not affected by p-aminohippurate (organic anion transporter inhibitor) or p-glycoprotein inhibitor (PSC833), but cell-to-lumen transport was reduced in a concentration-dependent manner by Mrp inhibitor MK571, leukotriene C4 (LTC4), azidothymidine (AZT), cAMP, and adefovir; the latter two compounds are Mrp4 substrates. Although MK571 and LTC4 reduced transport of the Mrp2 substrate fluorescein-methotrexate (FL-MTX), neither cAMP, adefovir, nor AZT affected FL-MTX transport. Fluo-cAMP transport was not reduced when tubules were exposed to endothelin-1, Na nitroprusside (an nitric oxide generator) or phorbol ester (PKC activator), all of which signal substantial reductions in cell-to-lumen FL-MTX transport. Fluo-cAMP transport was reduced by forskolin, and this reduction was blocked by the PKA inhibitor H-89. Finally, in membrane vesicles from Spodoptera frugiperda (Sf9) cells containing human MRP4, ATP-dependent and specific uptake of fluo-cAMP could be demonstrated. Thus, based on inhibitor specificity and regulatory signaling, cell-to-lumen transport of fluo-cAMP in killifish renal tubules is mediated by a transporter distinct from Mrp2, presumably a teleost form of Mrp4.

scholarly journals EFFECTS OF PROTEINURIA ON THE KIDNEY

1949 ◽  
Vol 89 (6) ◽  
pp. 643-668 ◽  
Author(s):  
James H. Baxter ◽  
George C. Cotzias
Keyword(s):  
Casein Hydrolysate ◽  
Blood Stream ◽  
Renal Tissue ◽  
Human Albumin ◽  
Tubular Damage ◽  
Renal Tubules ◽  
Rat Serum ◽  
Tubular Cells ◽  
Functional Studies ◽  
Renal Enlargement

Repeated intraperitoneal injections twice daily of various proteins into young rats were regularly accompanied by an increase in the protein content of the urine, significant renal enlargement, and often some degree of renal pallor. The most marked changes were induced by gelatin, followed in order by human albumin and bovine globulin. Rat serum produced similar but less conclusive changes. Similar changes were not produced by equivalent amounts of urea or casein hydrolysate. In sections from the kidneys of animals receiving gelatin, the cells of the convoluted tubules appeared enlarged, and they contained clear "spaces" throughout the cytoplasm. The tubular cells of the animals receiving the other solutions were not obviously altered in size or shape, and the cytoplasmic changes were slight or absent. There was little evidence of increased multiplication of cells or of tubular dilatation in the kidneys of any of the groups. Changes in concentrations of plasma proteins and hemoglobin, and the results of preliminary studies of the injected proteins in urine and renal tissue following the injections, are described and their possible significance discussed. It appears that the renal enlargement, as well as the increase in proteinuria and the tubular alterations which followed the protein injections, might have been caused in part by effects on the kidney of protein molecules per se, perhaps most likely by the effects on the tubular cells of an increased amount of protein filtered through the glomerular membranes, rather than entirely by effects of products of protein digestion and metabolism reaching the kidney through the blood stream. In the majority of animals there was no evidence from the morphological or functional studies, that the prolonged and continuous proteinuria induced by the protein injections resulted in renal damage, unless the renal enlargement, and the cytoplasmic changes which occurred regularly with gelatin, are considered evidence of damage. Renal enlargement and proteinuria promptly regressed after injections were discontinued. Lesions characterized by severe degrees of tubular damage, possibly as a result of tubular plugging, were observed in some of the animals of one group receiving gelatin solution of the usual concentration, and dilatation of renal tubules and glomerular capsules was present in some other gelatin-treated animals autopsied after relatively brief injection periods. A description is also presented of lesions of remarkable character which developed in the kidneys of all the animals of one small group receiving homologous serum obtained from severely anoxic donors. The possible relationship between the renal changes in the protein-injected animals and certain alterations of the kidneys observed in diseases characterized by large amounts of protein in the urine, is considered.

scholarly journals THE ROLE OF DISORGANIZATION OF CONNECTIVE TISSUE AND BASEMENT MEMBRANE OF RENAL TUBULAR EPITHELIUM IN THE PATHOGENESIS OF UROLITHIASIS

2020 ◽  
Vol 28 (2) ◽  
pp. 78-81
Author(s):  
Vadim A. Zubarev ◽  
Juliya M. Zabrodskaya ◽  
Anatoly I. Arkhangel'sky ◽  
Marlen E. Topuzov ◽  
Iliya V. Dovzhansky
Keyword(s):  
Connective Tissue ◽  
Basement Membrane ◽  
Stone Formation ◽  
Renal Tissue ◽  
The State ◽  
Renal Tubules ◽  
Renal Tubular Epithelium ◽  
Destructive Processes ◽  
Different Levels

According to a number of researchers, pathological changes in the structures of the renal papilla play a key role in the pathogenesis of urolithiasis. According to the results of existing studies, destructive processes in collagen are characterized by a change in the length, thickness of the fibers and their orientation in space. Collagen disorganization has enzymatic and non-enzymatic mechanisms. The aim of the study was to study the frequency and features of localization of Randall's plaques and morphological study of the state of connective tissue and the basement membrane of the epithelium of the renal tubules of different levels in urolithiasis. Microscopically studied preparations of 29 biopsies of renal tissue obtained from patients with fibro-uretero-pyeloscopy (11), percutaneous nephrolitholapaxy (18) and preparations obtained at 20 autopsies in cases without visible renal pathology. Histological preparations were stained with hematoxylin and eosin to determine the state of connective tissue fibers using Van Gieson, Mallory, Schiff-reagent, alcian blue, and Kos for calcifications. The results of the study show that the morphological signs of mucoid swelling of the connective tissue and basement membrane of the epithelium of the renal tubules of different levels in combination with the formation of microcalcifications require further study of the possible role of disorganization of the connective tissue of the renal stroma in the initiation of stone formation in the kidneys.

scholarly journals Protective Effects of Vitamin C Concomitant Treatment on Deferasirox-induced Renal Toxicity in Rats

2021 ◽  
Vol 23 (6) ◽  
pp. 926-943
Author(s):  
Taha Fereydouni ◽  
◽  
Saeed Hajihashemi ◽  
Parsa Yousefichaijan ◽  
Ali Rahbari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Creatinine Clearance ◽  
Renal Toxicity ◽  
Renal Tissue ◽  
Beta Thalassemia ◽  
Renal Tubules ◽  
Concomitant Treatment ◽  
Protective Effects ◽  
Antioxidant Power

Background and Aim: Deferasirox (Exjade) is an iron-chelating drug used in patients with beta-thalassemia major. Oxidative stress is among f the major causes of nephrotoxicity and its progression. Deferasirox, due to oxidative stress and increased cell apoptosis causes the dysfunction of renal tubules and renal toxicity. According to its antioxidant and anti-inflammatory properties, the present study explored the effect of vitamin C on deferasirox-induced kidney damage. Methods & Materials: This study was performed on 30 Wistar rats in 3 groups of control, deferasirox, and deferasirox plus vitamin C. To induce the nephrotoxicity, the intra-peritoneum injection of deferasirox (75 mg/kg/day) was used. After taking plasma from the blood samples of the explored rats, we determined the values of Cr, Na+, K+, Mg+, osmolality, and BUN in the obtained plasma and urine samples. The creatinine clearance, as well as the relative and absolute excretion of sodium and potassium, were also calculated. After separating the two kidneys, they were used for the histologic study with Hematoxylin and Eosin (H&E) staining, as well as Malondialdehyde (MDA) and Ferric Reducing Antioxidant Power (FRAP) biochemical studies. Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1396.309). Results: Cotreatment with deferasirox and vitamin C reduced renal tissue MDA and relative and absolute Na and K excretion and urine osmolarity; this method also increased creatinine clearance and renal tissue FRAP. Conclusion: The co-administration of vitamin C presented a significant protective effect on the renal toxicity induced by deferasirox. The protective property of deferasirox is because of the antioxidant impacts of vitamin C in reducing oxidative stress and lipid peroxidation.

scholarly journals Metabolic studies of rat renal tubule cells loaded with cystine: the cystine dimethylester model of cystinosis.

1995 ◽  
Vol 6 (2) ◽  
pp. 269-272
Author(s):  
J W Foreman ◽  
L L Benson ◽  
M Wellons ◽  
E D Avner ◽  
W Sweeney ◽  
...  
Keyword(s):  
Fanconi Syndrome ◽  
Renal Tubule ◽  
Renal Tissue ◽  
Renal Tubules ◽  
O2 Consumption ◽  
Metabolic Disturbances ◽  
Tissue Homogenates ◽  
Cystine Dimethylester ◽  
Tubule Cells ◽  
Isolated Rat

The cause of Fanconi syndrome in cystinosis is enigmatic. It has previously been shown that renal tubules could be loaded with cystine by incubating them with cystine dimethylester (CDE), mimicking the biochemical hallmark of cystinosis. Such tubules have impaired transport, decreased whole-cell O2 consumption, and substrate utilization. In this study, the metabolic disturbances in cystine-loaded renal tubule cells were further characterized. Isolated rat renal tubules were loaded with cystine by incubating them with 2 mM CDE for 10 min. This had no significant effect on total ATPase, Na(+)-K(+)-ATPase, or the ouabain-insensitive ATPase activity of renal tissue homogenates from these cystine-loaded tubules. Intracellular K was significantly lower in the cystine-loaded tubules (37 +/- 2 versus 47 +/- 3 nEq/mg; P < 0.008). Intracellular ATP was reduced by 39% in the cystine-loaded tubules (23.7 +/- 2.4 versus 38.1 +/- 3.3 nmol/mg of protein; P < 0.0025). CDE (2 mM) reduced isolated mitochondrial O2 consumption with glutamate as the substrate by 66% (4.7 +/- 0.7 versus 13.9 +/- 0.8 nm/min per mg of protein, P < 0.001) but had no effect on mitochondrial O2 consumption with succinate as the substrate. It was speculated that the impaired transport from cystine loading with CDE is secondary to a decrease in energy generation.

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