Carotenoids as a Source of Pigmentation in Juvenile Lobsters Fed a Purified Diet

1983 ◽  
Vol 40 (6) ◽  
pp. 699-704 ◽  
Author(s):  
Louis R. D'Abramo ◽  
Nancy A. Baum ◽  
Clark E. Bordner ◽  
Douglas E. Conklin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
De Novo ◽  
Spectrophotometric Analysis ◽  
Cod Liver Oil ◽  
De Novo Synthesis ◽  
Layer Chromatography ◽  
Β Carotene

Pigmentation of cultured lobsters is dependent upon the presence of dietary carotenoids. Inclusion of pure or crude carotenoids in a purified diet results in the accumulation of exoskeleton pigments tentatively identified by thin-layer chromatography and spectrophotometric analysis as primarily astaxanthin and β-carotene. Despite the lack of de novo synthesis pure carotenoids such as β-carotene, echinenone, and canthaxanthin are transformed into astaxanthin. The level of pigmentation produced by these biosynthetic precursors is related to the proximity to the astaxanthin end product. A carotenoid extract from crayfish waste dissolved in cod liver oil, and oleoresin paprika were effective in producing pigmentation. Rationale for the choice and use of carotenoid sources for incorporation into artificial feeds to produce natural pigmentation of cultured lobsters is discussed.

Carotenoids in Lichens From Tasmanian Forests

1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
First Time ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.

Notizen:Untersuchungen über Carotinoide in Gametangien und Sporen von Allomyces arbuscula/ Studies on Carotenoids in Gametangia and Spores of Allomyces arbuscula

1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux
Keyword(s):  
Thin Layer ◽  
Visible Light ◽  
Thin Layer Chromatography ◽  
Allomyces Arbuscula ◽  
Lag Period ◽  
Development Analysis ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.

Effect of feeding cod liver oil on the fate of radiotocopherol in sheep

1970 ◽  
Vol 48 (11) ◽  
pp. 751-757 ◽  
Author(s):  
M. Hidiroglou ◽  
K. J. Jenkins ◽  
J. R. Lessard ◽  
E. Borowsky
Keyword(s):  
Single Dose ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Experimental Period ◽  
Cod Liver Oil ◽  
The Difference ◽  
Layer Chromatography ◽  
Effect Of Feeding ◽  
Radioactivity Levels

The metabolism of a single dose of 3H-DL-α-tocopherol was studied in 16 sheep fed a dystrophy-producing hay alone or supplemented with cod liver oil. During the experimental period, which lasted for 5 days after the radioisotope dosing, radioactivity was higher in the rumen liquor of cod liver oil fed sheep than in those which received only hay. Significantly lower levels of radioactivity were found in the non-saponifiable material of various tissues of cod liver oil fed sheep than in the corresponding tissues of the unsupplemented animals. By means of column chromatography, thin layer chromatography, and radioautography the bulk of liver radioactivity was identified as unchanged α-tocopherol. Possible reasons for the difference in tissue radioactivity levels between cod liver oil fed and unsupplemented sheep are discussed.

scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

The carotenoids of the marigold, Tagetes erecta

1968 ◽  
Vol 46 (12) ◽  
pp. 1539-1541 ◽  
Author(s):  
A. U. Alam ◽  
J. R. Couch ◽  
C. R. Creger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spectral Methods ◽  
Tagetes Erecta ◽  
Absorption Studies ◽  
Layer Chromatography ◽  
Β Carotene

Pigments of Tagetes erecta petals have been separated by the use of multiple fractional extractions and thin-layer chromatography and analyzed by spectral methods. The petals showed a predominance of xanthophylls (98.7%) and only a small amount of carotenes. On the basis of absorption studies, lutein (64.1%), antheraxanthin (31.1 %), α-cryptoxanthin (3.1%), (β-carotene (0.6%), phytofluene (0.4%), and α-carotene (0.15%) have been characterized.

scholarly journals Investigations on carotenoids in Embryophyta. IV. The presence of apocarotenals in peatmosses

2014 ◽  
Vol 54 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Dry Weight ◽  
Layer Chromatography ◽  
Β Carotene

By means of column and thin-layer chromatography, the author investigated tit presence of various carotenoids in stems of 3 species of the Sphagnum genus. Apocarotenals (β-apo-2', β-apo-10' -carotenal and apo-12' -violaxanthal) and the following carotenoids were found: α-, β-, ;-carotene, β-cryptoxantin, lutein, lutein epoxide, zeaxanthin, adonixanthin, antheroxanthin, rhodoxanthin, rubixanthin, neoxanthin, vio-laxanthin and mutatoxanthin. The total content of carotenoids ranged from 11.954 to 41.579 mg•g<sup>-1</sup> dry weight.

scholarly journals Invesligations on carotenoids in fungi. IX. Dacrymycetaceae

2014 ◽  
Vol 16 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography ◽  
Β Carotene

The autor used column and thin-layer chromatography to determine the occurrence and quantitative relationships of particular carotenoids in fruit-bodies of the <i>Calocera viscosa</i> and <i>Dacrymyces deliquescens</i>. The predominant carotenoid was β-carotene and its derivatives.

scholarly journals Carotenoids in certain lichens of Białowieża Forest

2014 ◽  
Vol 63 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
Białowieża Forest ◽  
Carotenoid Composition ◽  
Xanthoria Parietina ◽  
Layer Chromatography ◽  
Cladonia Rangiferina ◽  
Β Carotene

Column-, and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 29 lichen species from Białowieża Forest: α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, 3'-epilutein, zeaxanthin, β-carotene monoepoxide, lutein epoxide, antheraxanthin, 3'-hydroxyechinenone, α-doradexanthin, canthaxanthin, astaxanthin, neoxani thin, violaxanthin and mutatoxanthin. The total content of carotenoids ranged from 16.83 (<i>Cladonia rangiferina</i>) to 92.98 µg g dry wt (<i>Xanthoria parietina</i>). There were differences in carotenoid composition, concentration of each carotenoid, and in the total content in the thalli of four species collected from niches with different insolation.

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