Chiasmata and chromosome breakages are related to crossing over in Drosophila ananassae males

Genome ◽  
2006 ◽  
Vol 49 (11) ◽  
pp. 1374-1383 ◽  
Author(s):  
Beatriz Goñi ◽  
Muneo Matsuda ◽  
Yoshiko N. Tobari
Keyword(s):  
Chromosome Pairing ◽  
Chiasma Frequency ◽  
Genetic Factors ◽  
Genetic Recombination ◽  
Paracentric Inversion ◽  
Drosophila Ananassae ◽  
Crossing Over ◽  
Low Frequencies ◽  
Genetic Crossing ◽  
Chromosome Breakages

A cytogenetic analysis of male crossing over in Drosophila ananassae revealed that cytological exchanges resulted in genetic crossing over, and that chiasma frequency and the genetic recombination correlated positively in chromosomes 2 and 3. Furthermore, the frequency of chromosome breakages correlated positively with chiasma frequency. Paracentric inversion heterozygosity had no detectable influence on the chromosome pairing or exchange events within the inversion loop at meiosis. Scoring of the chiasma demonstrated that males homozygous for the previously mapped enhancers of male crossing over had low frequencies of chiasmata, whereas higher frequencies of chiasmata were observed in males heterozygous for enhancers. The results presented here indicate that the genetic factors controlling male crossing over are involved in the origin of chromosome breakages and in exchange events.

THE RELATIONSHIP BETWEEN CHIASMATA AND CROSSING OVER IN TRITICUM AESTIVUM

Genetics ◽  
1973 ◽  
Vol 75 (2) ◽  
pp. 231-246
Author(s):  
T K Fu ◽  
E R Sears
Keyword(s):  
Chiasma Frequency ◽  
Rust Resistance ◽  
Genetic Recombination ◽  
Substantial Reduction ◽  
Leaf Rust Resistance ◽  
Crossing Over ◽  
Two Temperatures ◽  
Independent Behavior ◽  
The Relationship

ABSTRACT Telocentrics for the β arm of chromosome 4A and the long arm of 6B were used as cytological markers for the determination of chiasma frequency. In concomitant studies of recombination, terminal segments of rye and T. umbellulatum chromatin carrying Hp (Hairy peduncle) and Lr9 (Leaf-rust resistance), respectively, marked 4A and 6B. Two temperatures, 21° and 32°, were used for both the 4A and 6B experiments.—Only one chiasma was observed in each heteromorphic bivalent. Because there was a substantial reduction in pairing between diakinesis and metaphase I, all determinations of chiasma frequency were made at diakinesis. In the 21° experiments, agreement was good between genetic recombination and cytological prediction on the basis of the partial chiasmatypy hypothesis that each chiasma represents a crossover. At 32° both chiasma frequency and crossing over, but particularly the latter, were strongly reduced. The fewer crossovers than expected are explained in part by stickiness of chromosomes at the high temperature, sometimes resulting in adjacent chromosomes being wrongly scored as having a chiasma, and in part by premetaphase disjunction of some recombined bivalents and subsequent independent behavior of the two resulting univalents.—Male transmission of the 4A telocentric from the heteromorphic bivalent was unusually high: 51% at 21° and 31% at 32°.

Crossing over does occur in males of Drosophila ananassae from natural populations

Genome ◽  
2012 ◽  
Vol 55 (7) ◽  
pp. 505-511 ◽  
Author(s):  
Beatriz Goñi ◽  
Muneo Matsuda ◽  
Masa-Toshi Yamamoto ◽  
Carlos R. Vilela ◽  
Yoshiko N. Tobari
Keyword(s):  
Natural Populations ◽  
Drosophila Ananassae ◽  
Crossing Over ◽  
Cytological Analysis ◽  
Wild Type ◽  
Cytological Evidence ◽  
Type Strains ◽  
Chromosome Breakages ◽  
Brazilian Populations

Spontaneous crossing over in males of Drosophila ananassae has been well demonstrated using F1 individuals from crosses between marker stocks and wild type strains. However, the question of its occurrence in males from natural populations remained open. Here we present the cytological evidence that crossing over does occur in males of D. ananassae from two Brazilian populations, sampled nearly 21 years apart, and in two recently sampled populations, one from Indonesia and one from Okinawa, Japan. Cytological analysis of meiosis in males collected from nature and in sons of females from the same population inseminated in nature revealed the presence of chiasmata, inversion chiasmata, and isosite chromosome breakages in the diplotene cells in all sampled populations. These data demonstrate that reciprocal and nonreciprocal exchanges and chromosome breakages, previously reported as related events of male crossing over, do occur at variable frequencies among males from natural populations.

scholarly journals GENETIC CONTROL OF MEIOSIS IN RICE, ORYZA SATIVA L. III. EFFECT OF DS GENES ON GENETIC RECOMBINATION

Genetics ◽  
1984 ◽  
Vol 108 (3) ◽  
pp. 697-706
Author(s):  
Kunio Kitada ◽  
Takeshi Omura
Keyword(s):  
Oryza Sativa ◽  
Chiasma Frequency ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Oryza Sativa L ◽  
The Other ◽  
Crossing Over ◽  
Homozygous Condition ◽  
Synaptonemal Complexes ◽  
The Mean

ABSTRACT The recombination frequency as influenced by five independent recessive ds genes was measured on three segments of different chromosomes of rice, Oryza sativa L. Each ds gene in the homozygous condition resulted in an almost equally reduced recombination frequency in the three segments. When the mean reduction in recombination frequency was related to the reduction of chiasma frequency, the five ds genes were divided into two types: in one type the reduction of chiasma frequency almost corresponded to the mean reduction of recombination frequency, and in the other the chiasma frequency was greatly reduced in comparison with the mean reduction of recombination frequency. Three of the five ds genes were found to belong to the former group. In both types, normal synaptonemal complexes were observed in pachytene cells homozygous for ds genes. This finding suggests that ds genes do not affect the formation of synaptonemal complexes which are regarded as the prerequisite structure for crossing over.

scholarly journals Male recombination in Brazilian populations of Drosophila ananassae

Genome ◽  
2016 ◽  
Vol 59 (7) ◽  
pp. 493-500 ◽  
Author(s):  
Beatriz Goñi ◽  
Muneo Matsuda ◽  
Yoshiko N. Tobari
Keyword(s):  
Genetic Control ◽  
Genetic Factors ◽  
Regional Distribution ◽  
High Variability ◽  
Drosophila Ananassae ◽  
Crossing Over ◽  
Male Recombination ◽  
Spontaneous Recombination ◽  
Recombination Value ◽  
Brazilian Populations

With few exceptions, spontaneous crossing over does not normally occur in male Drosophila. Drosophila ananassae males show considerable amounts of crossing over. In wild males of D. ananassae from Asian (2008) and Brazilian populations (1986 and 2007) variable frequencies of meiotic crossing over, estimated from chiasmata counts, suggested the existence of factors controlling male crossing over in these populations. To corroborate for such prediction, we present data on spontaneous recombination in F1 males of D. ananassae heterozygous for chromosomes of the same Brazilian populations (1986) and marker chromosomes using three testers stocks. Mean recombination value was low, although high variability existed between individual frequencies. Recombination frequencies between lines in each tester stock were not significantly different, excepting when the 3ple-px and 3ple-cy testers were compared (p < 0.05). These two testers differ in respect to the regional distribution of crossovers. The occurrence of recombination in chromosomes 2 and 3 in F1 males tested with e65 se; bri ru was not related, suggesting they are under independent genetic control. Our data are consistent with proposed genetic factors controlling male crossing over in the tester stocks and to the presence of enhancers and suppressors of male crossing over segregating in the Brazilian populations (1986).

Synthesis and cytological characterization of trigeneric hybrids of durum wheat with and without Ph1

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Prem P Jauhar ◽  
M Doğramaci ◽  
T S Peterson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Genetic Recombination ◽  
Genomic In Situ Hybridization ◽  
Homoeologous Pairing ◽  
Alien Gene Transfer ◽  
Chromosome 5B ◽  
Alien Gene ◽  
Trigeneric Hybrids

Wild grasses in the tribe Triticeae, some in the primary or secondary gene pool of wheat, are excellent reservoirs of genes for superior agronomic traits, including resistance to various diseases. Thus, the diploid wheatgrasses Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve (2n = 2x = 14; JJ genome) and Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE genome) are important sources of genes for disease resistance, e.g., Fusarium head blight resistance that may be transferred to wheat. By crossing fertile amphidiploids (2n = 4x = 28; JJEE) developed from F1 hybrids of the 2 diploid species with appropriate genetic stocks of durum wheat, we synthesized trigeneric hybrids (2n = 4x = 28; ABJE) incorporating both the J and E genomes of the grass species with the durum genomes A and B. Trigeneric hybrids with and without the homoeologous-pairing suppressor gene, Ph1, were produced. In the absence of Ph1, the chances of genetic recombination between chromosomes of the 2 useful grass genomes (JE) and those of the durum genomes (AB) would be enhanced. Meiotic chromosome pairing was studied using both conventional staining and fluorescent genomic in situ hybridization (fl-GISH). As expected, the Ph1-intergeneric hybrids showed low chromosome pairing (23.86% of the complement), whereas the trigenerics with ph1b (49.49%) and those with their chromosome 5B replaced by 5D (49.09%) showed much higher pairing. The absence of Ph1 allowed pairing and, hence, genetic recombination between homoeologous chromosomes. Fl-GISH analysis afforded an excellent tool for studying the specificity of chromosome pairing: wheat with grass, wheat with wheat, or grass with grass. In the trigeneric hybrids that lacked chromosome 5B, and hence lacked the Ph1 gene, the wheat–grass pairing was elevated, i.e., 2.6 chiasmata per cell, a welcome feature from the breeding standpoint. Using Langdon 5D(5B) disomic substitution for making trigeneric hybrids should promote homoeologous pairing between durum and grass chromosomes and hence accelerate alien gene transfer into the durum genomes.Key words: alien gene transfer, chiasma (xma) frequency, chromosome pairing, fluorescent genomic in situ hybridization (fl-GISH), homoeologous-pairing regulator, specificity of chromosome pairing, wheatgrass.

Concluding remarks

1977 ◽  
Vol 277 (955) ◽  
pp. 371-376 ◽  
Keyword(s):  
Genetic Recombination ◽  
Meiotic Behaviour ◽  
Chromosome Organization ◽  
Base Sequence ◽  
Crossing Over ◽  
Primary Target ◽  
Sequence Complexity ◽  
A Genome ◽  
Chromosome Alignment ◽  
Single Pattern

Professor Darlington opened the meeting by challenging us with the view that chromosomes made the laws of heredity, rather than heredity fashioning the organization of chromosomes. To keep this wheel of logic spinning, it may be said that chromosomes also made the process of meiosis and thus determined the laws of meiotic exchange. I choose this gambit because our discussions lent considerable emphasis to the view that chromosome complexity compels its own sets of distinctive, and perhaps varied, mechanisms to effect the ultimate event of molecular recombination. The complexity that leads molecular recombination to operate in elaborate meiotic moulds is not, it should be emphasized, base sequence complexity. On the contrary, sequence repeats and genetic homoeologies, though adding disproportionately little to the base sequence complexity of a genome, adds considerably to the complexity of effecting chromosome alignment and crossing over. How chromosomes of diverse genetic content manage that complexity and in the process mould the characteristics of meiotic behaviour has been the primary target of our deliberations. That no single pattern of meiotic conduct was perceived in consequence of the discussions, is to be expected. To the extent that genomes differ in various aspects of chromosome organization - and that they do is patent - the particulars of meiotic organization might also differ. Although a strong sentiment was occasionally expressed for a single universal process of meiosis, it is my opinion that sameness and universality may be mistakenly treated as synonyms. Universals provide for diversity; they do not impose sameness. The task of identifying universal threads among different meiotic fabrics is not a straightforward one. The ultimate act of genetic recombination offers no detailed guide to the routes by which it may be achieved. Indeed, it is the structure of the chromosome that dictates the route ; recombination only signals the direction.

Genetic Recombination in Coprinus

1970 ◽  
Vol 6 (3) ◽  
pp. 669-678
Author(s):  
B. C. LU
Keyword(s):  
Fruiting Body ◽  
Temperature Effects ◽  
Cold Shock ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Temperature Treatment ◽  
Sensitive Period ◽  
Crossing Over ◽  
Synaptinemal Complex ◽  
Before And After

The frequency of genetic recombination in Coprinus lagopus may be modified by heat and cold shock. By removal of samples from a fruiting body before and after temperature treatment, it is possible to study the ultrastructure of chromosomes at the time recombination frequency (between den+ x +me-1) can be modified. The sensitive period for temperature effects and, therefore, probably the time of crossing over, commences with the formation of the synaptinemal complex (S.C.) and ends with its disappearance, i.e. during the entire existence of the S.C. It is concluded that recombination is an event subsequent to the formation of the S.C. and is independent of the process of its formation. It is suggested that the event takes place at the synaptic centre.

scholarly journals Synaptonemal complex antigen location and conservation.

1987 ◽  
Vol 105 (1) ◽  
pp. 93-103 ◽  
Author(s):  
P B Moens ◽  
C Heyting ◽  
A J Dietrich ◽  
W van Raamsdonk ◽  
Q Chen
Keyword(s):  
Chromosome Pairing ◽  
Meiotic Prophase ◽  
Structural Integrity ◽  
Genetic Recombination ◽  
Positive Control ◽  
Western Blots ◽  
Lateral Element ◽  
Homologous Chromosomes ◽  
Recombination Nodules ◽  
Grain Distribution

The axial cores of chromosomes in the meiotic prophase nuclei of most sexually reproducing organisms play a pivotal role in the arrangement of chromatin, in the synapsis of homologous chromosomes, in the process of genetic recombination, and in the disjunction of chromosomes. We report an immunogold analysis of the axial cores and the synaptonemal complexes (SC) using two mouse monoclonal antibodies raised against isolated rat SCs. In Western blots of purified SCs, antibody II52F10 recognizes a 30- and a 33-kD peptide (Heyting, C., P. B. Moens, W. van Raamsdonk, A. J. J. Dietrich, A. C. G. Vink, and E. J. W. Redeker, 1987, Eur. J. Cell Biol., 43: 148-154). In spreads of rat spermatocyte nuclei it produces gold grains over the cores of autosomal and sex chromosomes. The cores label lightly during the chromosome pairing stage (zygotene) of early meiotic prophase and they become more intensely labeled when they are parallel aligned as the lateral elements of the SC during pachytene (55 grains/micron SC). Statistical analysis of electronically recorded gold grain positions shows that the two means of the bimodal gold grain distribution coincide with the centers of the lateral elements. At diplotene, when the cores separate, the antigen is still detected along the length of the core and the enlarged ends are heavily labeled. Shadow-cast SC preparations show that recombination nodules are not labeled. The continued presence suggests that the antigens serve a continuing function in the cores, such as chromatin binding, and/or structural integrity. Antibody III15B8, which does not recognize the 30- and 33-kD peptides, produces gold grains predominantly between the lateral elements. The grain distribution is bimodal with the mean of each peak just inside the pairing face of the lateral element. The antigen is present where and while the cores of the homologous chromosomes are paired. From the location and the timing, it is assumed that the antigen recognized by III15B8 functions in chromosome pairing at meiotic prophase. The two anti-rat SC antibodies label rat and mouse SCs but not rabbit or dog SCs. A positive control using human CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) anti-centromere serum gives equivalent labeling of SC centromeres in the rat, mouse, rabbit, and dog. It is concluded that the SC antigens recognized by II52F10 and III15B8 are not widely conserved. The two antibodies do not bind to cellular or nuclear components of somatic cells.(ABSTRACT TRUNCATED AT 400 WORDS)

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