SOLUBILISATION D'ENZYMES HYDROLYTIQUES CHEZ GOSSYPIUM HIRSUTUM, G. ANOMALUM ET DES DERIVES DE L'HYBRIDATION ENTRE CES DEUX ESPECES

1969 ◽  
Vol 11 (3) ◽  
pp. 582-586 ◽  
Author(s):  
J. B. Vieira da Silva ◽  
Ch. Poisson
Keyword(s):  
Acid Phosphatase ◽  
Osmotic Stress ◽  
Gossypium Hirsutum ◽  
Hydrolytic Enzymes ◽  
Stress Conditions ◽  
Chromosome 3 ◽  
Hereditary Material ◽  
Wild Parent ◽  
Segregating Population

Hybrid derivatives between Gossypium anomalum and G. hirsutum, both 2n + 1 aneuploids for chromosome 3 of G. anomalum and euploids derived from this segregating population, exhibit under osmotic stress conditions the same pattern of solubilization of the hydrolytic enzymes, β-fructofuranosidase, β-amylase and acid phosphatase, as the wild parent G. anomalum.This biochemical relationship and the slightly changed phenotype, suggest that the hereditary material could have been transferred from G. anomalum to the euploids.

Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population

2014 ◽  
Vol 65 (2) ◽  
pp. 139 ◽  
Author(s):  
Gastón Quero ◽  
Lucía Gutíerrez ◽  
Ramiro Lascano ◽  
Jorge Monza ◽  
Niels Sandal ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Root Growth ◽  
Stress Condition ◽  
Stress Conditions ◽  
Chromosome 1 ◽  
Chromosome 3 ◽  
Growth Responses ◽  
Parental Allele ◽  
Ionic Stress ◽  
Ril Population

The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.

scholarly journals Silicon Stimulates Plant Growth and Metabolism in Rice Plants under Conventional and Osmotic Stress Conditions

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 777
Author(s):  
Sara Monzerrat Ramírez-Olvera ◽  
Libia Iris Trejo-Téllez ◽  
Fernando Carlos Gómez-Merino ◽  
Lucero del Mar Ruíz-Posadas ◽  
Ernesto Gabriel Alcántar-González ◽  
...  
Keyword(s):  
Plant Growth ◽  
Osmotic Stress ◽  
Abiotic Factors ◽  
Stress Conditions ◽  
Chlorophyll B ◽  
Rice Seedlings ◽  
Root Volume ◽  
Total Sugars ◽  
Dry Biomass ◽  
Peg 8000

Exogenous silicon (Si) can enhance plant resistance to various abiotic factors causing osmotic stress. The objective of this research was to evaluate the application of 1 and 2 mM Si to plants under normal conditions and under osmotic stress. Morelos A-98 rice seedlings, were treated with 1 and 2 mM SiO2 for 28 d. Subsequently, half of the plants were subjected to osmotic stress with the addition of 10% polyethylene glycol (PEG) 8000; and continued with the addition of Si (0, 1 and 2 mM SiO2) for both conditions. The application of Si under both conditions increased chlorophyll b in leaves, root volume, as well as fresh and dry biomass of roots. Interestingly, the number of tillers, shoot fresh and dry biomass, shoot water content, concentration of total chlorophyll, chlorophyll a/b ratio, and the concentration of total sugars and proline in shoot increased with the addition of Si under osmotic stress conditions. The addition of Si under normal conditions decreased the concentration of sugars in the roots, K and Mn in roots, and increased the concentration of Fe and Zn in shoots. Therefore, Si can be used as a potent inorganic biostimulant in rice Morelos A-98 since it stimulates plant growth and modulates the concentration of vital biomolecules and essential nutrients.

scholarly journals In vitro seed germination response of corn hybrids to osmotic stress conditions

age ◽  
2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Charles Hunt Walne ◽  
Annabeth Gaudin ◽  
W. Brien Henry ◽  
Kambham Raja Reddy
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Stress Conditions ◽  
Corn Hybrids ◽  
Germination Response

scholarly journals iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions

2019 ◽  
Vol 10 ◽  
Author(s):  
Tinghong Ming ◽  
Lingxin Geng ◽  
Ying Feng ◽  
Chenyang Lu ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Osmotic Stress ◽  
Stress Conditions ◽  
Proteomic Profiling

The blood leukocytes of Bufo marinus: a light, phase-contrast, and histochemical study

1987 ◽  
Vol 65 (6) ◽  
pp. 1445-1453 ◽  
Author(s):  
M. Samuel Cannon ◽  
H. W. Sampson ◽  
E. D. Kapes
Keyword(s):  
Acid Phosphatase ◽  
Phase Contrast ◽  
Periodic Acid ◽  
Hydrolytic Enzymes ◽  
Neutral Red ◽  
Bufo Marinus ◽  
Oxidative Enzymes ◽  
Blood Leukocytes ◽  
Periodic Acid Schiff ◽  
Aerobic And Anaerobic

Blood leukocytes of Bufo marinus were studied by light and phase-contrast microscopy and histochemical techniques for the localization of glycogen, lipids, several basic proteins, and a number of hydrolytic and oxidative enzymes. The hydrolytic enzymes occurred in varying amounts in neutrophils, eosinophils, lymphocytes, and monocytes; neutrophils were the only leukocytes to demonstrate alkaline phosphatase activity, while β-glucuronidase was only seen in lymphocytes, and aryl-sulfatase was not observed in any leukocytes. Periodic acid – Schiff (PAS) positive granules also occurred in varying amounts in leukocytes. Slight lipid activity was only seen in neutrophils, while arginine, and (or) lysine, and tyrosine reactivity was only observed in eosinophils. The appearance and histochemical reactivity of acid phosphatase granules in neutrophils corresponded closely with the appearance and number of specific neutrophilic granules seen in Wright–Giemsa preparations and with the PAS-positive granules. Small lymphocytes were myeloperoxidase (peroxidase) negative; β-glucuronidase, acid phosphatase, and PAS-positive granules corresponded to neutral red granules seen in supravital films. The oxidative enzymes also occurred in differing amounts in leukocytes, but strongly suggested that the leukocytes of Bufo marinus are capable of some degree of aerobic and anaerobic metabolism.

Proteolysis in Cotyledon Cells of Phaseolus vulgaris L.: Changes in Multiple Hydrolase Activities Following Germination

1979 ◽  
Vol 6 (4) ◽  
pp. 467 ◽  
Author(s):  
JA Crump ◽  
DR Murray
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Hydrolytic Enzymes ◽  
Maximum Activity ◽  
Hydrolase Activity ◽  
Phaseolus Vulgaris L ◽  
Maximum Value ◽  
Cotyledon Cells

Changes in activity of several hydrolytic enzymes were measured in extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder). Aminopeptidase AP2 (phenanthroline-sensitive leucyl β-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively. By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl β-naphthylamidase) showed maximum activity after 2 days and declined immediately. α-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining. Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities, and that neither activity indicates the presence of a chymotrypsin-like endopeptidase.

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