INTERACTION BETWEEN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA IN MIXED CULTURES

1965 ◽  
Vol 11 (6) ◽  
pp. 947-952 ◽  
Author(s):  
R. K. Guthrie ◽  
B. H. Cooper ◽  
J. K. Ferguson ◽  
H. E. Allen
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Carbohydrate Metabolism ◽  
Mixed Cultures ◽  
Pure Cultures ◽  
Stimulation Of

Both continuous and stationary cultures were used to detect interactions between Escherichia coli and Pseudomonas aeruginosa in mixed cultures, with pure cultures included as controls. The interactions observed were both pH and substrate dependent. Stimulation of P. aeruginosa in mixture was the most marked interaction, and was, in part, due to changes in carbohydrate metabolism.

Changes in the activity of antifungals in mixed cultures of bacteria and yeasts

1981 ◽  
Vol 27 (8) ◽  
pp. 843-846 ◽  
Author(s):  
M. H. Domon ◽  
L. G. Mathieu ◽  
J. de Repentigny
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhimurium ◽  
Direct Effect ◽  
Culture Medium ◽  
Antifungal Agents ◽  
Mixed Cultures ◽  
Sterol Metabolism ◽  
Pure Cultures

When Candida cells were grown in mixed cultures with Escherichia coli, Salmonella typhimurium, or Pseudomonas aeruginosa, their sensitivity to antifungal agents affecting sterol metabolism or functioning (polyenes and miconazole) was increased by several log units. This phenomenon was overcome by the addition to the culture medium of sugars utilized either by the bacteria or the yeast. Antifungals without direct effect on sterol metabolism (5-fluorocytosine, methylparaben, and 4-hydroxyquinazoline) were not more active in mixed than in pure cultures.

In Vitro Inhibition of the Growth of Salmonella typhimurium and Escherichia coli 0157:H7 by Bacteria Isolated from the Cecal Contents of Adult Chickens

1991 ◽  
Vol 54 (7) ◽  
pp. 496-501 ◽  
Author(s):  
ARTHUR HINTON ◽  
GEORGE E. SPATES ◽  
DONALD E. CORRIER ◽  
MICHAEL E. HUME ◽  
JOHN R. DELOACH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Salmonella Typhimurium ◽  
Mixed Cultures ◽  
Enterococcus Durans ◽  
E Coli ◽  
Pure Cultures ◽  
In Vitro Inhibition ◽  
Lactic Acids

A Veillonella species and Enterococcus durans were isolated from the cecal contents of adult broilers. Mixed cultures of Veillonella and E. durans inhibited the growth of Salmonella typhimurium and Escherichia coli 0157:H7 on media containing 2.5% lactose (w/v). The growth of S. typhimurium or E. coli 0157:H7 was not inhibited by mixed cultures containing Veillonella and E. durans on media containing only 0.25% lactose or by pure cultures of Veillonella or E. durans on media containing either 0.25% or 2.5% lactose. The mixed cultures of Veillonella and E. durans produced significantly (P<0.05) more acetic, propionic, and lactic acids in media containing 2.5% lactose than in media containing 0.25% lactose. The inhibition of the enteropathogens was related to the production of lactic acid from lactose by the E. durans and the production of acetic and propionic acids from lactic acid by the Veillonella.

scholarly journals In Vitro Activity of Trovafloxacin against Bacteroides fragilis in Mixed Culture with either Escherichia coli or a Vancomycin- Resistant Strain of Enterococcus faecium Determined by an Anaerobic Time-Kill Technique

2001 ◽  
Vol 45 (1) ◽  
pp. 243-251 ◽  
Author(s):  
Lorna E. T. Stearne ◽  
Clarissa Kooi ◽  
Wil H. F. Goessens ◽  
Irma A. J. M. Bakker-Woudenberg ◽  
Inge C. Gyssens
Keyword(s):  
Escherichia Coli ◽  
Resistant Strain ◽  
Enterococcus Faecium ◽  
Mixed Cultures ◽  
E Coli ◽  
Abdominal Abscesses ◽  
Pure Cultures ◽  
Vancomycin Resistant ◽  
Time Kill

ABSTRACT To determine the efficacy of trovafloxacin as a possible treatment for intra-abdominal abscesses, we have developed an anaerobic time-kill technique using different inocula to study the in vitro killing ofBacteroides fragilis in pure culture or in mixed culture with either Escherichia coli or a vancomycin-resistant strain of Enterococcus faecium (VREF). With inocula of 5 × 105 CFU/ml and trovafloxacin concentrations of ≤2 μg/ml, a maximum observed effect (E max) of ≥6.1 (log10 CFU/ml) was attained with all pure and mixed cultures within 24 h. With inocula of 108CFU/ml, a similar E max and a similar concentration to produce 50% of E max(EC50) for B. fragilis were found in both pure cultures and mixed cultures with E. coli. However, to produce a similar killing of B. fragilis in the mixed cultures with VREF, a 14-fold increase in the concentration of trovafloxacin was required. A vancomycin-susceptible strain of E. faecium and a trovafloxacin-resistant strain of E. coli were also found to confer a similar “protective” effect on B. fragilis against the activity of trovafloxacin. Using inocula of 109 CFU/ml, the activity of trovafloxacin was retained for E. coli and B. fragilis and was negligible against VREF. We conclude that this is a useful technique to study the anaerobic killing of mixed cultures in vitro and may be of value in predicting the killing of mixed infections in vivo. The importance of using mixed cultures and not pure cultures is clearly shown by the difference in the killing of B. fragilis in the mixed cultures tested. Trovafloxacin will probably be ineffective in the treatment of infections involving large numbers of enterococci. However, due to its ability to retain activity against large cultures of B. fragilis and E. coli, trovafloxacin could be beneficial in the treatment of intra-abdominal abscesses.

scholarly journals Comparative Studies on Antibacterial Efficacy of Natural & Artificial Honey on Isolated Pathogens from Burnt Wound Sample

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 269-275
Author(s):  
Vijaya Benerji G ◽  
Anantha Lakshmi Y ◽  
Shankar K ◽  
Punitha V C
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Pathogen ◽  
Isolation And Characterization ◽  
Wound Contamination ◽  
Nutrient Agar Medium ◽  
Pure Cultures ◽  
Made In

The present study dealt with the Isolation and Characterization of a bacterial pathogen from a burnt wound sample. The burnt Wound contamination may be a major muddling previously, patients. Blazed wounds indicated essentially higher bacterial tallies compared for the normal wound. The burnt wound sample was inoculated using nutrient agar medium and medium and incubated at 37oC for 24 hrs. After incubation pure cultures of organisms were made in respective agar mediums. The pure culture of bacteria was identified and characterized as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. These organisms are dangerous pathogens penetrating the wound, producing a lot of pain and exudates. The improvement of blazed wound contamination is not another wonder and is a progressing issue for numerous individuals. Open wounds serve by a passage course to sullying 28 with attacking microorganisms. Furthermore Postponed wound recuperating about contaminated smolder wounds; it will be connected with expanded discomfort, healing center sit tight and human services expenses. The introduce fill in on the honey demonstrated antibacterial movement against the blazed wound pathogenic microscopic organisms for example, such that escherichia coli, staphylococcus aureus Furthermore Pseudomonas aeruginosa. It will meet the requirements of essential products required in humans, animals as well as in the pharmaceutical industry. The natural honey is more effective and safer than artificial honey and the antibiotics against the burnt wound pathogens.

Interactions of Pseudomonas aeruginosa in predominant biofilm or planktonic forms of existence in mixed culture with Escherichia coli in vitro

2013 ◽  
Vol 59 (9) ◽  
pp. 604-610 ◽  
Author(s):  
Marina V. Kuznetsova ◽  
Irina L. Maslennikova ◽  
Tamara I. Karpunina ◽  
Larisa Yu. Nesterova ◽  
Vitaly A. Demakov
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Total Concentration ◽  
Single Species ◽  
Mixed Cultures ◽  
Interspecies Interactions ◽  
E Coli ◽  
Predominant Form ◽  
The One

Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in diverse niches. In this study we examined the influence of the predominant form of cell existence of and the exometabolite production by P. aeruginosa strains on interspecies interactions, in vitro. Bacterial numbers of P. aeruginosa and E. coli in mixed plankton cultures and biofilms compared with their numbers in single plankton cultures and biofilms changed in a different way, but were in accordance with the form of P. aeruginosa cell existence. The mass of a mixed-species biofilm was greater than the mass of a single-species biofilm. Among the mixed biofilms, the one with the “planktonic” P. aeruginosa strain had the least biomass. The total pyocyanin and pyoverdin levels were found to be lower in all mixed plankton cultures. Despite this, clinical P. aeruginosa strains irrespective of the predominant form of existence (“biofilm” or “planktonic”) had a higher total concentration of exometabolites than did the reference strain in 12–24 h mixed cultures. The metabolism of E. coli, according to its bioluminescence, was reduced in mixed cultures, and the decrease was by 20- to 100-fold greater with the clinical Pseudomonas strains than the reference Pseudomonas strain. Thus, both the predominant form of existence of and the exometabolite production by distinct P. aeruginosa strains should be considered to fully understand the interspecies relationship and bacteria survival in natural communities.

Increased in vitro sensitivity of Candida albicans to amphotericin B when grown in mixed culture with Escherichia coli

1978 ◽  
Vol 24 (12) ◽  
pp. 1482-1489 ◽  
Author(s):  
L. G. Mathieu ◽  
D. Dube ◽  
M. Lebrun
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Mixed Culture ◽  
Mixed Cultures ◽  
Polyene Antibiotics ◽  
E Coli ◽  
Pure Cultures

The growth of Candida albicans was inhibited by some Escherichia coli strains both in conventional batch cultures and also in a chemostat under conditions of constant addition of fresh medium. Concentrations of 0.2 μg amphotericin B per millilitre and of 2 μg nystatin per millilitre, which caused a slight inhibition of C. albicans in pure culture, exerted a strong fungicidal effect when the yeast was placed in mixed cultures with certain strains of E. coli. Candida albicans cells, inhibited by either E. coli or in mixed culture with polyene antibiotics, appeared larger and less uniformly stained by acridine orange than control cells from pure cultures. Addition of chloramphenicol to the mixed cultures, in quantities sufficient to kill the E. coli cells, abolished the increased sensitivity of C. albicans to amphotericin B or nystatin. In preliminary in vivo tests, E. coli did not sensitize C. albicans to the polyene antibiotics.

The effect of phenazine methosulfate-ascorbate on bacterial active transport and adenosine triphosphate formation: inhibition of Pseudomonas aeruginosa and stimulation of Escherichia coli

1979 ◽  
Vol 25 (7) ◽  
pp. 798-802 ◽  
Author(s):  
R. G. Eagon ◽  
T. W. Hodge III ◽  
J. B. Rake ◽  
J. M. Yarbrough
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Active Transport ◽  
Adenosine Triphosphate ◽  
Electron Donor ◽  
Energy Sources ◽  
Phenazine Methosulfate ◽  
Atp Formation ◽  
Stimulation Of ◽  
Donor System

The artificial electron-donor system, phenazine methosulfate (PMS) ascorbate, inhibited active transport of glucose by Pseudomonas aeruginosa irrespective of whether the incubation systems were in air, flushed with oxygen, or gassed with nitrogen under anaerobic denitrifying conditions. Active transport of glucose by P. aeruginosa was also inhibited by reduced 5-N-methyl-phenazonium-3-sulfonate, a membrane-impermeable electron donor. PMS–ascorbate caused rapid depletion of intracellular adenosine triphosphate (ATP) when added to respiring cell suspensions of P. aeruginosa either in the presence or absence of glucose or succinate as oxidizable energy sources. In contrast, under identical conditions, Escherichia coli formed ATP with PMS–ascorbate as the sole oxidizable energy source and ATP formation continued when glucose or succinate was present in addition to PMS–ascorbate in the incubation system.

Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts

2019 ◽  
Vol 5 (3) ◽  
pp. 1-10
Author(s):  
Puspa Julistia Puspita ◽  
Mega Safithri ◽  
Nirmala Peni Sugiharti
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Agent ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Leaf Extracts ◽  
Herbal Plants

Piper crocatum is one of medicinal herbal plants with a large number of benefits. Usually herbal plants have activity as antibacterial agent. Therefore, the objectives of this research were to obtain information on antibacterial activities of the leaf extracts of Piper crocatum againts four types of bacteria, in that Staphylococcus, Bacillus substilis, Escherichia coli, and Pseudomonas aeruginosa and then to analyze the phytochemistry of the leaf extracts of Piper crocatum. The leaves of Piper crocatum were extracted by maceration and reflux using ethanol 30%. The assays of the antibacterial activities and phytochemistry on the extracts were carried out using the method of Maria Bintang. Results showed that the yield of the extraction using ethanol by maceration method was 20.8%. Meanwhile, using the reflux method, the yield was obtained about 26.25%. The phytochemistry analysis showed that the leaf extracts of Piper crocatum contained alkaloid, steroid and tanin. According to this study, it was found that the leaf extract of Piper crocatum can be used to inhibit the growth of B. subtilis and P. aeuruginosa, but can not inhibit the growth of E.coli and S. aureus.

Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Providencia Rettgeri ◽  
E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

Activité antibactérienne de l’huile essentielle de Pelargonium x asperum et son potentiel synergique avec la nisine

2019 ◽  
Vol 17 (3) ◽  
pp. 140-148 ◽  
Author(s):  
A. Ouelhadj ◽  
L. Ait Salem ◽  
D. Djenane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nous Avons ◽  
Activité Antibactérienne

Ce travail vise l’étude de l’activité antibactérienne de l’huile essentielle (HE) de Pelargoniumx asperum et de la bactériocine, la nisine seul et en combinaison vis-à-vis de six bactéries dont quatre sont multirésistantes d’origine clinique. L’activité antibactérienne in vitro a été évaluée par la méthode de diffusion sur gélose. La concentration minimale inhibitrice (CMI) est aussi déterminée pour HE. Les résultats ont révélé une activité antibactérienne significative exercée par HE visà-vis de Staphylococcus aureus (ATCC 43300), Staphylococcus aureus et Escherichia coli avec des diamètres d’inhibition de 36,00 ; 22,50 et 40,00 mm, respectivement. Cependant, l’HE de Pelargonium asperum a montré une activité antibactérienne supérieure par rapport à la nisine. Les valeurs des CMI rapportées dans cette étude sont comprises entre 1,98–3,96 μl/ml. Les combinaisons réalisées entre HE et la nisine ont montré un effet additif vis-à-vis de Escherichia coli (ATCC 25922) avec (50 % HE Pelargonium asperum + 50 % nisine). Par contre, nous avons enregistré une synergie vis-à-vis de Klebsiella pneumoniae avec (75 % HE Pelargonium asperum + 25 % nisine) et contre Pseudomonas aeruginosa avec les trois combinaisons testées. Les résultats obtenus permettent de dire que l’HE de Pelargonium asperum possède une activité antibactérienne ainsi que sa combinaison avec la nisine pourrait représenter une bonne alternative pour la lutte contre l’antibiorésistance.

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