The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. I. Immunizing properties

1978 ◽  
Vol 24 (2) ◽  
pp. 117-123 ◽  
Author(s):  
B. B. Diena ◽  
F. E. Ashton ◽  
A. Ryan ◽  
R. Wallace ◽  
M. B. Perry
Keyword(s):  
Animal Model ◽  
Neisseria Gonorrhoeae ◽  
Chicken Embryo ◽  
Common Antigen ◽  
Colony Type ◽  
Chemically Modified

The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoeae colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 μg of LPS, and challenged intracerebrally with 10–20 LD50's of N. gonorrhoeae colony type 1. Immunized mice were significantly protected (P < 0.01 to < 0.05) against challenge with different T1 isolates of N. gonorrhoeae when compared with non-immunized mice. Mice, injected with succinylated or alkali-treated LPS were not protected against gonococcal challenges.In a second animal model, leghorn hens were immunized intravenously with three injections of 500 μg of LPS followed by a booster of 2.5 mg 2 weeks later. Embryonated eggs obtained from immunized hens were protected against challenge with 5 × 103 – 1 × 104 LD50's of three different T1 isolates. When hens were injected with the chemically modified LPS, the embryos were not resistant to gonococcal challenge. The results of this study demonstrate the ability of R-type gonococcal LPS to provide protection against different T1 isolates of N. gonorrhoeae.

The Lipopolysaccharides of Neisseria gonorrhoeae Colony Types 1 and 4

1975 ◽  
Vol 53 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Benito B. Diena ◽  
Fraser E. Ashton ◽  
Rebecca Wallace
Keyword(s):  
Molecular Weight ◽  
Neisseria Gonorrhoeae ◽  
High Molecular Weight ◽  
Lipid A ◽  
Core Oligosaccharide ◽  
Colony Type ◽  
Mild Hydrolysis

The lipopolysaccharides (LPS) of strains of Neisseria gonorrhoeae grown in type 1 (T1) and 4 (T4) colony forms have been isolated. LPS from T4 colony type cells on mild hydrolysis gave a lipid A and a core oligosaccharide composed of 2-amino-2-deoxy-D-glucose, D-glucose, D-galactose, L-glycero-D-manno-heptose and 3-deoxy-D-manno-octulosonic acid that appeared to be common to all the strains examined. LPS from T1 colony type cells on mild hydrolysis gave a lipid A and high molecular weight O polysaccharides which showed considerable differences in glycose composition for each strain examined. In those strains examined, T4 cells appear to produce a common 'R' type LPS whereas T1 cells produce an 'S' type LPS with structurally different O polysaccharide structures which probably account for serologically differentiated strains of N. gonorrhoeae.

The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. II. Use of hen antiserum to gonococcal lipopolysaccharide in a rapid slide test for the identification of N. gonorrhoeae from primary isolates and secondary cultures

1978 ◽  
Vol 24 (2) ◽  
pp. 124-128 ◽  
Author(s):  
R. Wallace ◽  
F. E. Ashton ◽  
A. Ryan ◽  
B. B. Diena ◽  
C. Malysheff ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Rapid Identification ◽  
Cross Reactivity ◽  
Agglutination Test ◽  
Common Antigen ◽  
Colony Type ◽  
Neisseria Lactamica ◽  
Slide Agglutination Test ◽  
Slide Test ◽  
Branhamella Catarrhalis

An antiserum has been prepared in hens to R-type gonococcal lipopolysaccharide (LPS) and used in a simple slide-agglutination test for the identification of Neisseria gonorrhoeae. Anti-LPS serum agglutinated gonococcal cells representative of the four colony types of N. gonorrhoeae. Absorption of the antiserum with LPS removed the agglutinating activity. Secondary cultures (1120) were tested without observation of the colony type and all were agglutinated. No agglutination occurred with strains of Neisseria meningitidis, Neisseria lactamica, non-pathogenic Neisseria, Pseudomonas aeruginosa, Branhamella catarrhalis, or with species of lactobacilli and Acinetobacter. Cross-reactivity of the antiserum occurred with some streptococci. The anti-LPS serum was used to identify N. gonorrhoeae in primary isolates from the cervix, urethra, and pharynx. Of 251 gonococcal isolates tested, 249 were agglutinated by the antiserum, while all of the corresponding secondary cultures were agglutinated. The antiserum did not agglutinate N. meningitidis found in primary isolates from pharyngeal specimens. Anti-LPS hen serum should be useful for the rapid identification of N. gonorrhoeae in primary isolates or secondary cultures.

scholarly journals Isolation and characterization of a rough colony type of Neisseria gonorrhoeae

1977 ◽  
Vol 5 (3) ◽  
pp. 365-369
Author(s):  
N F Jacobs ◽  
S J Kraus ◽  
C Thornsberry ◽  
J Bullard
Keyword(s):  
Electron Microscopy ◽  
Neisseria Gonorrhoeae ◽  
Primary Cultures ◽  
Irregular Surface ◽  
Colony Type ◽  
Isolation And Characterization ◽  
Distinctive Morphology ◽  
Rough Form

A new colony type of Neisseria gonorrhoeae was detected in the primary cultures from 8 of 180 men with gonococcal urethritis. This colony type contrasts with those previously described by having a rough and irregular surface. In six of the eight cases, the rough form predominated. The distinctive morphology of the rough colony variant could be maintained indefinitely by selective subculture. By electron microscopy, organisms taken from rough colonies of each of the eight isolates were piliated. Antimicrobial susceptibilities of type 1 and rough clones derived from the same patients were identical for ampicillin, penicillin, tetracycline, and spectinomycin. After inoculation of rough colonies into subcutaneous chambers in mice and guinea pigs, type 1 colonies predominated in cultures of material obtained from the chambers. This new piliated colony type of N. gonorrhoeae may provide an opportunity to investigate factors other than pili that contribute to gonococcal virulence.

Anti-inflammatory effects of C-peptide on kidney of type 1 diabetes mellitus animal model

2019 ◽  
Vol 47 (1) ◽  
pp. 721-726 ◽  
Author(s):  
Michelle T. Alves ◽  
Amanda C. S. Chaves ◽  
Ana Paula M. Almeida ◽  
Ana Cristina Simões e Silva ◽  
Stanley de A. Araújo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Animal Model ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
C Peptide ◽  
Anti Inflammatory

Cultivation and properties of Neisseria sp. grown in chemically defined media

1972 ◽  
Vol 18 (7) ◽  
pp. 1087-1090 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Present Report ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Specific Antisera

Neisseria Chemically Defined Medium (NCDM) has been used routinely in our laboratory for a variety of purposes. The present report describes the development of NCDM agar, wherein the NCDM base is sterilized by filtration and defined supplements and agar are added. The medium is transparent and both meningococci and gonococci grow within 72 h. When grown on NCDM agar, Types 2 and 3 gonococcal colonies tend to revert to Type 1. The serological grouping of meningococci with specific antisera is not affected by growth on this medium.Parallel investigations on the growth of these species in liquid NCDM demonstrated that the yield of Neisseria gonorrhoeae is enhanced when the medium is sterilized by filtration.

Differential regulation of glucose transporter isoforms by the src oncogene in chicken embryo fibroblasts

1991 ◽  
Vol 11 (9) ◽  
pp. 4448-4454
Author(s):  
M K White ◽  
T B Rall ◽  
M J Weber
Keyword(s):  
Glucose Transport ◽  
Chicken Embryo ◽  
Glucose Transporter ◽  
Sequence Similarity ◽  
Transporter Protein ◽  
Mrna Induction ◽  
Embryo Fibroblasts ◽  
Type 3 ◽  
Chicken Embryo Fibroblasts

The increase in glucose transport that occurs when chicken embryo fibroblasts (CEFs) are transformed by src is associated with an increase in the amount of type 1 glucose transporter protein, and we have previously shown that this effect is due to a decrease in the degradation rate of this protein. The rate of CEF type 1 glucose transporter biosynthesis and the level of its mRNA are unaffected by src transformation. To study the molecular basis of this phenomenon, we have been isolating chicken glucose transporter cDNAs by hybridization to a rat type 1 glucose transporter probe at low stringency. Surprisingly, these clones corresponded to a message encoding a protein which has most sequence similarity to the human type 3 glucose transporter and which we refer to as CEF-GT3. CEF-GT3 is clearly distinct from the CEF type 1 transporter that we have previously described. Northern (RNA) analysis of CEF RNA with CEF-GT3 cDNA revealed two messages of 1.7 and 3.3 kb which were both greatly induced by src transformation. When the CEF-GT3 cDNA was expressed in rat fibroblasts, a three-to fourfold enhancement of 2-deoxyglucose uptake was observed, indicating that CEF-GT3 is a functional glucose transporter. Northern analyses using a CEF-GT3 and a rat type 1 probe demonstrated that there is no hybridization between different isoforms but that there is cross-species hybridization between the rat type 1 probe and the chicken homolog. Southern blot analyses confirmed that the chicken genomic type 1 and type 3 transporters are encoded by distinct genes. We conclude that CEFs express two types of transporter, type 1 (which we have previously reported to be regulated posttranslationally by src) and a novel type 3 isoform which, unlike type 1, shows mRNA induction upon src transformation. We conclude that src regulates glucose transport in CEFs simultaneously by two different mechanisms.

scholarly journals Lipooligosaccharides (LOS) of Neisseria gonorrhoeae and Neisseria meningitidis have components that are immunochemically similar to precursors of human blood group antigens. Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human erythrocytes.

1988 ◽  
Vol 168 (1) ◽  
pp. 107-126 ◽  
Author(s):  
R E Mandrell ◽  
J M Griffiss ◽  
B A Macher
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Incubation Temperature ◽  
Human Erythrocytes ◽  
Human Infant ◽  
Blood Group Antigens ◽  
Sequence Specificity ◽  
Adult Humans ◽  
Branched Chain

We have used mouse mAbs, 3F11 and 06B4, that are specific for highly conserved epitopes of Neisseria gonorrhoeae lipooligosaccharides (LOS) to identify immunochemically similar structures on human erythrocytes. mAb 3F11 agglutinated erythrocytes from all randomly selected adult humans, while mAb 06B4 agglutinated only 80% of the same specimens. The antibodies had an activity with erythrocytes similar to human cold agglutinins in that agglutination occurred at 4 degrees C and decreased with increasing incubation temperature. Human infant erythrocytes were agglutinated less well, but enzymatic treatment of either infant or adult cells resulted in an increase in expression of the 3F11- and 06B4-defined epitopes. Both antibodies bound to a series of neutral glycosphingolipids from human erythrocytes and neutrophils that have a type 2 (Gal beta 1----4GlcNAc) or N-acetyllactosamine structure. Neither antibody bound to glycosphingolipids from human meconium, which have a type 1 (Gal beta 1----3GlcNAc) structure. The antibodies were unable to bind to N-acetyl-lactosamine glycosphingolipids with a nonreducing terminal sialic acid or a Gala1----3Gal disaccharide. Antibody binding also was blocked by the presence of fucose linked to the penultimate glucosamine residue of N-acetyllactosamine glycosphingolipids. Although both antibodies bound to linear and branched-chain N-acetyllactosamine glycosphingolipids, 3F11 had a higher affinity for branched structures than did 06B4. The activity of 3F11 with human adult and infant treated and untreated erythrocytes with N-acetyllactosamine glycosphingolipids, and with LOS was very similar, if not identical, in specificity to 1B2, an mAb prepared from mice inoculated with a linear N-acetyllactosamine glycosphingolipid.

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