Dissolved organic matter utilization and oxygen uptake in algal–bacterial microcosms

1979 ◽  
Vol 25 (11) ◽  
pp. 1315-1320 ◽  
Author(s):  
David L. Tison ◽  
A. J. Lingg
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Algal Biomass ◽  
Algal Species ◽  
Chlorella Pyrenoidosa ◽  
Individual Species ◽  
Bacterial Respiration ◽  
Bacterial Microbiota ◽  
Limiting Nutrient ◽  
Algal Photosynthesis

Under closed laboratory conditions, at non-limiting nutrient levels, the biomass of Anabaena variabilis. Anacystis nidnlans, Chlorella pyrenoidosa. and Selanastrum capricornutum increased with increasing levels of dissolved organic matter (DOM) as a result of bacterially produced carbon dioxide (CO2) and (or) cofactors. Oxygen (O2) produced as a result of algal photosynthesis was sufficient to supply the majority of O2 required by the bacterial community. The percentage of DOM utilized by bacteria which was subsequently incorporated into algal biomass varied with individual species indicating that the association between individual algal species and the bacterial microbiota varied.Under natural conditions bacteria could provide CO2 and (or) cofactors for algal photosynthesis which in turn supplies O2 for bacterial respiration. This mutualistic association in aquatic environments could result in an increase in planktonic and epiphytic algal biomass if other nutrients are available.

Dissolved organic matter as P source for biofilms in two contrasting low-order streams

2019 ◽  
Vol 193 (2) ◽  
pp. 131-142
Author(s):  
Verónica Díaz-Villanueva
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Deciduous Forest ◽  
Spatial Scales ◽  
Algal Species ◽  
Community Level ◽  
Nutrient Concentrations ◽  
Organic Substrates ◽  
One Year ◽  
Forest Streams

Forest streams receive large amounts of leaves whose leachates are an important source of dissolved organic matter (DOM), providing not only carbon but also organic nutrients to the microbial communities in streams. I carried out a field study to evaluate the effect of different DOM concentrations on the biofilm structure and functional traits in two similar forest streams belonging to the same catchment. I compared biofilm biomass and nutri- ent content throughout one year, algal species composition, and biofilm community-level physiological profiles in two streams with different DOM concentration and aromaticity. Dissolved nutrient concentrations were higher in the stream with higher DOM concentration, with a concomitant higher biofilm biomass, and there was also a temporal pattern, with higher values during the autumn. Phosphorus content in biofilms was also higher in the high DOM stream, coincidently with a higher capacity of the community to utilize organic P source (glucose-1-P) as a substrate. In contrast, the biofilms from the stream with lower DOM concentrations preferentially used N-organic substrates (amino acids and amines). These results reveal that the biofilms of forest streams make use of organic matter nutrients, so that streams with different DOM loads may differ in biofilm biomass due to changes in both bacterial and autotrophic biomass. In addition, biofilm dynamics may be related to forest phenology, as the highest OM input in this deciduous forest is represented by tree leaves, which supply DOM through leachates, and in particular, with P-rich leachates. In conclusion, different DOM concentrations in two nearby streams led to differences in the community-level physiological profile, as has been previously demonstrated at larger spatial scales in oceans, lakes and along larger rivers.

Evaluation of algal photosynthesis inhibition activity for dissolved organic matter with the consideration of inorganic and coloring constituents

Chemosphere ◽  
2019 ◽  
Vol 224 ◽  
pp. 333-342 ◽  
Author(s):  
Kentaro Misaki ◽  
Yuhei Morita ◽  
Kentaro Kobayashi ◽  
Yuichi Sugawara ◽  
Yoshihisa Shimizu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Inhibition Activity ◽  
Photosynthesis Inhibition ◽  
Algal Photosynthesis

Bacterial response to dissolved organic matter affects resource availability for algae

2005 ◽  
Vol 62 (2) ◽  
pp. 472-481 ◽  
Author(s):  
Jennifer L Klug
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Resource Availability ◽  
Inorganic Carbon ◽  
Algal Growth ◽  
Carbon Limitation ◽  
Bacterial Respiration ◽  
Photochemical Degradation ◽  
Nitrogen And Phosphorus ◽  
Bacterial Response

In aquatic systems, the presence of colored dissolved organic matter (DOM) may affect algal growth in numerous ways. This paper focuses on the effects of DOM on resource availability. DOM contains nitrogen and phosphorus, which may become available following microbial or photochemical degradation. Also, addition of DOM may stimulate bacterial growth, which in turn may change the availability of nitrogen, phosphorus, and inorganic carbon to algae. Experiments conducted in a moderately colored lake showed that the effect of DOM on algal growth depended on the amount of nutrients present in the peat extract and on bacterial response to DOM. There was evidence for competition for phosphorus between algae and bacteria in some treatments. In addition, when both bacteria growth and algal growth were high, bacterial respiration of DOM alleviated algal carbon limitation by providing algae with an inorganic carbon source. Thus, the degree to which bacteria are stimulated by the addition of DOM will affect the amount of phosphorus and inorganic carbon available for algal growth. These results suggest that part of the difficulty in predicting algal response to changes in DOM and nutrient concentration may be due partially to variability in bacterial responses.

scholarly journals Dissolved Organic Matter Modulates Algal Oxidative Stress and Membrane System Responses to Binary Mixtures of Nano-Metal-Oxides (nCeO2, nMgO and nFe3O4) and Sulfadiazine

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 712 ◽  
Author(s):  
Fan Zhang ◽  
Nan Ye ◽  
Se Wang ◽  
Yue Meng ◽  
Hao Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Metal Oxides ◽  
Emerging Contaminants ◽  
Scenedesmus Obliquus ◽  
Algal Species ◽  
Cell Membrane Permeability ◽  
Freshwater Microalgae ◽  
Membrane Function

Joint biomarker responses, oxidative stress and membrane systems, were determined for nano-metal-oxides (nMeO, i.e., nCeO2, nMgO, and nFe3O4) and sulfadiazine (SDZ) exposed at relevant low concentrations to two freshwater microalgae Scenedesmus obliquus and Chlorella pyrenoidosa. The impacts of dissolved organic matter (DOM) on the joint biomarker responses were also investigated. Results indicated that the presence of SDZ significantly decreased the level of intercellular reactive oxygen species (ROS) in the algal cells exposed to each nMeO. Reduction of cell membrane permeability (CMP) and mitochondrial membrane potential (MMP) in the algal cells was observed when the algae were exposed to the mixture of SDZ and the nMeO. The degree of reduction of the ROS level, CMP, and MMP significantly went down with the addition of DOM to a certain extent. Changes in cellular oxidative stress and membrane function depended on the types of both nMeO and algal species. This contribution provides an insight into the hazard assessment of a mixture consisting of emerging contaminants and DOM, as they can coexist in the aquatic environment.

scholarly journals Enhanced bioavailability of dissolved organic matter (DOM) in human-disturbed streams in Alpine fluvial networks

2022 ◽  
Vol 19 (1) ◽  
pp. 187-200
Author(s):  
Thibault Lambert ◽  
Pascal Perolo ◽  
Nicolas Escoffier ◽  
Marie-Elodie Perga
Keyword(s):  
Land Use ◽  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Human Disturbance ◽  
Human Activities ◽  
Urban Streams ◽  
Bacterial Respiration ◽  
Inland Waters ◽  
C Cycle ◽  
The Impact

Abstract. The influence of human activities on the role of inland waters in the global carbon (C) cycle is poorly constrained. In this study, we investigated the impact of human land use on the sources and biodegradation of dissolved organic matter (DOM) and its potential impact on bacterial respiration in 10 independent catchments of the Lake Geneva basin. Sites were selected along a gradient of human disturbance (agriculture and urbanization) and were visited twice during the winter high-flow period. Bacterial respiration and DOM bioavailability were measured in the laboratory through standardized dark bioassays, and the influence of human land uses on DOM sources, composition and reactivity was assessed from fluorescence spectroscopy. Bacterial respiration was higher in agro-urban streams but was related to a short-term bioreactive pool (0–6 d of incubation) of autochthonous origin, whose relative contribution to the total DOM pool increased with the degree of human disturbance. On the other hand, the degradation of a long-term (6–28 d) bioreactive pool related to terrestrial DOM was independent from the catchment land use and did not contribute substantially to aquatic bacterial respiration. From a greenhouse gas emission perspective, our results suggest that human activities may have a limited impact on the net C exchanges between inland waters and the atmosphere, as most CO2 fixed by aquatic producers in agro-urban streams is cycled back to the atmosphere after biomineralization. Although seasonal and longitudinal changes in DOM sources must be considered, the implications of our results likely apply more widely as a greater proportion of autochthonous-DOM signature is a common feature in human-impacted catchments. Yet, on a global scale, the influence of human activities remains to be determined given the large diversity of effects of agriculture and urbanization on freshwater DOM depending on the local environmental context.

scholarly journals No evidence of a human influence on the mineralization of terrestrial dissolved organic matter (DOM) in Alpine fluvial networks

2021 ◽  
Author(s):  
Thibault Lambert ◽  
Pascal Perolo ◽  
Nicolas Escoffier ◽  
Marie-Elodie Perga
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Human Disturbance ◽  
Human Activities ◽  
Bacterial Respiration ◽  
Inland Waters ◽  
Land Uses ◽  
Lake Geneva ◽  
Basin Scale ◽  
The Impact

Abstract. The influence of human activities on the role of inland waters in the global carbon (C) cycle is poorly constrained. In this study, we investigated the impact of human land use on the degradation of terrestrial dissolved organic matter (DOM) and its potential impact on bacterial respiration in ten independent catchments of the Lake Geneva Basin. Sites were selected along a gradient of human disturbance (agriculture and urbanization), and we monitored bacterial respiration in parallel to DOM bioavailability. Fluorescence spectroscopy was used to determine the influence of human land uses on DOM sources and composition as well as the dynamic of degradation or production of the fluorophores identified in our study sites. Higher bacterial respiration measured in agro-urban streams related to a short-term bioreactive pool (0–6 days of incubation) from autochthonous origin, whose the size increased with human disturbance. On the other hand, the degradation of terrestrial DOM was not impacted by human activities and was not found to contribute substantially to aquatic bacterial respiration. Although human land uses controlled DOM sources, composition and bioavailability at the basin scale, our results showed that human activities in the Lake Geneva Basin had a limited impact on the net C exchanges between inland waters and the atmosphere related to DOM mineralization. Considering that greater proportion of autochthonous-DOM signature is a common feature in human-impacted catchments, the implications of our results likely apply more widely. Yet, on a global scale, the influence of human activities remains to be determine given the large diversity of effects of agriculture and urbanization on freshwater DOM depending on the local environmental context.

Sign in / Sign up

Export Citation Format

Share Document