β-Glucuronidase Isoenzymes of Rat-Liver Lysosomes
1973 ◽
Vol 51
(7)
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pp. 973-979
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Keyword(s):
β-Glucuronidase (β-D-glucuronide glucuronohydrolase, EC 3.2.1.31) from liver lysosomes of male Sprague–Dawley rats was purified by (NH4)2SO4 precipitation, treatment with trypsin and gel filtration on Sephadex G-200, and then fractionated into five active components by ion-exchange chromatography on DEAE-Sephadex A-25 or DEAE-cellulose. All five isoenzymes were shown to be electrophoretically and ultracentrifugally homogeneous. Their sedimentation coefficients range from 12.27 to 13.39. All five isoenzymes appear to be glycoproteins.
1980 ◽
Vol 58
(4)
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pp. 331-335
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1984 ◽
Vol 62
(6)
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pp. 449-455
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2013 ◽
Vol 16
(4)
◽
pp. 85-91
2010 ◽
Vol 2010
◽
pp. 1-8
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