The metabolism of 3-deoxy-3-fluoro-D-glucose by Locusta migratoria and Schistocerca gregaria

1977 ◽  
Vol 55 (4) ◽  
pp. 369-375 ◽  
Author(s):  
A. Romaschin ◽  
N. F. Taylor ◽  
D. A. Smith ◽  
D. Lopes
Keyword(s):  
Chromatographic Analysis ◽  
Schistocerca Gregaria ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Competitive Inhibitor ◽  
Sorbitol Dehydrogenase ◽  
Kinetic Rate ◽  
Temperature Programmed ◽  
Isothermal Gas ◽  
Sorbitol Metabolism

3-Deoxy-3-fluoro-D-glucose (3FG) administered by injection is toxic to adult Locusta migratoria or Schistocerca gregaria (LD50, 4.8 mg/g). Temperature-programmed and isothermal gas chromatographic analysis of poisoned locust haemolymph reveals the presence of a fluorinated metabolite identified as 3-deoxy-3-fluoro-D-glucitol (3FGL). The enzymes responsible for the accumulation of this metabolite are located in the fat body of the insect and partially purified as aldose reductase (alditol: NADP+ 1-oxidoreductase, EC 1.1.1.21) and sorbitol dehydrogenase (L-iditol: NAD+ 5-oxidoreductase, EC 1.1.1.14). 3FGL is shown to be both a competitive inhibitor of the NAD-linked sorbitol dehydrogenase with Ki 8.2 × 10−2 M as well as a substrate with Km 0.5 M. A kinetic rate equation is derived and verified to account for the kinetic duality of 3FGL. These results partially explain the toxic effects of 3FG and are consistent with, the presence of a hitherto undetected sorbitol metabolism in locusts.

Incorporation of 3-deoxy-3-fluoro-D-glucose into glycogen and trehalose in fat body and flight muscle in Locusta migratoria

1986 ◽  
Vol 6 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Moses Agbanyo ◽  
Norman F. Taylor
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Chromatographic Analysis ◽  
Flight Muscle ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Tissue Extracts ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Flight muscle and fat body extracts from Locusta migratoria were incubated with D-[U-14C]-glucose or D-[3-3H]-3-deoxy-3-fluoroglucose and the products were analyzed. In the case of the latter compound, radio-chromatographic analysis yielded glycogen and trehalose fractions that were shown by19F nuclear magnetic resonance to contain fluorine. Acid hydrolysis of these fractions liberated tritium labelled 3-deoxy-3-fluoro-D-glucose. In addition to the formation of “fluoroglycogen” and “fluorotrehalose” in these tissue extracts, there was an accumulation of tritium labelled fructose.

The Structure and Development of Microbodies in the Fat Body and other Tissues of an Insect (Calpodes Ethlius)

1970 ◽  
Vol 28 ◽  
pp. 148-149
Author(s):  
M. Locke ◽  
J. T. McMahon
Keyword(s):  
Electron Microscopy ◽  
Liquid Crystals ◽  
Fat Body ◽  
Competitive Inhibitor ◽  
Dense Core ◽  
Urate Oxidase ◽  
Blood Proteins ◽  
Free Base ◽  
The Core ◽  
The Matrix

The fat body of insects has always been compared functionally to the liver of vertebrates. Both synthesize and store glycogen and lipid and are concerned with the formation of blood proteins. The comparison becomes even more apt with the discovery of microbodies and the localization of urate oxidase and catalase in insect fat body.The microbodies are oval to spherical bodies about 1μ across with a depression and dense core on one side. The core is made of coiled tubules together with dense material close to the depressed membrane. The tubules may appear loose or densely packed but always intertwined like liquid crystals, never straight as in solid crystals (Fig. 1). When fat body is reacted with diaminobenzidine free base and H2O2 at pH 9.0 to determine the distribution of catalase, electron microscopy shows the enzyme in the matrix of the microbodies (Fig. 2). The reaction is abolished by 3-amino-1, 2, 4-triazole, a competitive inhibitor of catalase. The fat body is the only tissue which consistantly reacts positively for urate oxidase. The reaction product is sharply localized in granules of about the same size and distribution as the microbodies. The reaction is inhibited by 2, 6, 8-trichloropurine, a competitive inhibitor of urate oxidase.

scholarly journals Human erythrocyte sorbitol metabolism and the role of sorbitol dehydrogenase

Diabetologia ◽  
1988 ◽  
Vol 31 (10) ◽  
pp. 766-770 ◽  
Author(s):  
Y. Nagasaka ◽  
S. Fujii ◽  
T. Kaneko
Keyword(s):  
Human Erythrocyte ◽  
Sorbitol Dehydrogenase ◽  
Sorbitol Metabolism

THE POTENTIAL OF BACTERIA FOR THE MICROBIAL CONTROL OF GRASSHOPPERS AND LOCUSTS

1997 ◽  
Vol 129 (S171) ◽  
pp. 147-156 ◽  
Author(s):  
B. Zelazny ◽  
M.S. Goettel ◽  
B. Keller
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Thuringiensis ◽  
Serratia Marcescens ◽  
Schistocerca Gregaria ◽  
Locusta Migratoria ◽  
Microbial Control ◽  
Control Agent ◽  
Promising Agent ◽  
Laboratory Populations ◽  
The Many

AbstractBacteria have been implicated in disease epizootics observed in field populations and laboratory-reared locusts and grasshoppers. Two species [Serratia marcescens Bizio and Pseudomonas aeruginosa (Schroeter) Migula] consistently infect locusts when ingested with food and can spread in laboratory populations. However, research on developing these organisms for microbial control of locusts and grasshoppers begun in the 1950s has not been continued. In recent years strains of Bacillus thuringiensis Berliner have been studied for activity against locusts and grasshoppers. Results of additional trials by the authors are reported. Among 393 B. thuringiensis isolates and 93 preparations of other sporeforming bacteria fed to nymphs of Locusta migratoria (L.) and/or Schistocerca gregaria Forsk., none has shown any pathogenicity to the insects. The recent discovery of novel B. thuringiensis strains active against various diverse pests and the many properties of a sporeforming bacterium that satisfy the requirements for a microbial control agent, and the development of Serratia entomophila as a promising agent for control of grass grubs, provide incentive to continue the search for an orthopteran-active sporeforming bacterium and to re-investigate the potential of non-sporeforming bacterial pathogens as microbial control agents of grasshoppers and locusts.

scholarly journals Screening and potent applicability analysis of commonly used pesticides against desert locust: an integrative entomo-informatics approach

2020 ◽  
Author(s):  
Anik Banik ◽  
Md. Fuad Mondal ◽  
Md. Mostafigur Rahman Khan ◽  
Sheikh Rashel Ahmed ◽  
Md. Mehedi Hasan
Keyword(s):  
Binding Affinity ◽  
Large Scale ◽  
Schistocerca Gregaria ◽  
Indian Subcontinent ◽  
Locusta Migratoria ◽  
Field Trials ◽  
The Other ◽  
List Type ◽  
Agricultural Pesticides ◽  
Global Threat

AbstractThe locust problem is a global threat for food security. Locusts can fly and migrate overseas within a zip and creating a large-scale devastation to the diversified agro-ecosystem. GIS based analysis showed the recent movement of locusts, among them Schistocerca gregaria and Locusta migratoria are predominant in Indian subcontinent and are found more notorious and devastating one. This devastation needs to be stopped to save human race from food deprivation. In our study, we screened some commonly used agricultural pesticides and strongly recommended three of them viz. biphenthrin, diafenthiuron and silafluofen which might be potential to control the desert locusts based on their binding affinity towards the locust’s survival proteins. Our phylogenetic analysis reveals that these three recommended pesticides might also show potency to the other locust species as well as they are also way safer than the other commercially available pesticides. These proposed pesticide’s bioactive analogs from fungus and bacteria may also show efficacy as next generation controlling measures of locust as well as different kind of pests. These recommended pesticides are expected to be highly effective against locusts and needs to bring forward by the entomologists’ by performing experimental field trials.HighlightsGIS map unmasked the 2020 migratory pattern of locusts which now predominant towards Indian subcontinent.Biphenthrin, diafenthiuron and silafluofen showed maximum binding affinity.Biphenthrin and diafenthiuron were relatively safer than silafluofen.Bioactive analogs from fungus and bacteria could be an alternative to control locusts.Pesticides inhibition hotspots for desert locusts were unrevealed.

Amino-Acid Metabolism in Locust Tissues

1957 ◽  
Vol 34 (2) ◽  
pp. 276-289
Author(s):  
B. A. KILBY ◽  
ELISABETH NEVILLE
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Schistocerca Gregaria ◽  
Fat Body ◽  
Malpighian Tubule ◽  
Malpighian Tubules ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Mitochondrial Fractions

1. Homogenates of fat-body of Schistocerca gregaria Forsk. were shown to catalyse transamination reactions between α-ketoglutarate and numerous α-amino acids. The aspartate/glutamate and alanine/glutamate transaminases were the most active. They were present in both the ‘soluble’ and the mitochondrial fractions of fat-body cells and also in Malpighian tubules and mid-gut wall. The other transaminases in the fat-body were confined to the mitochondrial fraction. 2. Fat-body, Malpighian tubule and mid-gut wall homogenates were able to convert glutamic acid into glutamine, a compound which could also act as an amino-group donor in some transamination reactions. 3. A glutamate-cytochrome c reductase system which involved diphosphopyridine nucleotide was present in fat-body. 4. Fat-body contained an active arginase, but urease could not be detected. A D-amino-acid oxidase was present, together with a less active L-amino-acid oxidase. 5. In general, it appears that amino-acid metabolism in the locust resembles that in higher animals.

scholarly journals The mechanism of C-20 hydroxylation of α-ecdysone in the desert locust, Schistocerca gregaria

1977 ◽  
Vol 168 (3) ◽  
pp. 513-520 ◽  
Author(s):  
P Johnson ◽  
H H Rees
Keyword(s):  
Schistocerca Gregaria ◽  
Fat Body ◽  
Difference Spectrum ◽  
Maximum Activity ◽  
Malpighian Tubules ◽  
Desert Locust ◽  
Ph Optimum ◽  
Developmental Variation ◽  
Moulting Hormone ◽  
Cytochrome P 450

1. The C-20 hydroxylation of alpha-ecdysone to produce beta-ecdysone was investigated in the desert locust, Schistocerca gregaria. 2. alpha-Ecdysone C-20 hydroxylase activity was located primarily in the fat-body and Malpighian tubules. The properties of the hydroxylation system from Malpighian tubules investigated further. 3. The enzyme system was mitochondrial, had a pH optimum of 6.5, an apparent Km of 12.5 micron and required O2 and NADPH. 4. The activity of the hydroxylation system showed developmental variation within the fifth instar, the maximum activity corresponding to the maximum tire of endogenous moulting hormone. The significance of these results is assessed in relation to the control of the endogenous titre of beta-ecdysone. 5. The mechanism of the hydroxylation system was investigated by using known inhibitors of hydroxylation reactions such as CO, metyrapone and cyanide. 6. The CO difference spectrum of the reduced mitochondrial preparation indicated the presence of cytochrome P-450 in the preparation. 7. It concluded that the alpha-ecdysone C-20 hydroxylase system is a cytochrome P-450-deendent mono-oxygenase.

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