The inhibition of tetrahydrofuran clathrate-hydrate formation with antifreeze protein

2003 ◽  
Vol 81 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
H Zeng ◽  
L D Wilson ◽  
V K Walker ◽  
J A Ripmeester
Keyword(s):  
Induction Time ◽  
Crystal Morphology ◽  
Choristoneura Fumiferana ◽  
Hydrate Formation ◽  
Antifreeze Protein ◽  
Clathrate Hydrate ◽  
Inhibition Mechanism ◽  
Type I ◽  
Inhibition Activity ◽  
Hydrate Crystal

The effect of Type I fish antifreeze protein (AFP) from the winter flounder, Pleuronectes americanus (Walbaum), (WfAFP) on the formation of tetrahydrofuran (THF) clathrate hydrate was studied by observing changes in THF crystal morphology and determining the induction time for nucleation. AFP retarded THF clathrate-hydrate growth at the tested temperatures and modified the THF clathrate-hydrate crystal morphology from octahedral to plate-like. AFP appears to be even more effective than the kinetic inhibitor, polyvinylpyrrolidone (PVP). Recombinant AFP from an insect, a spruce budworm, Choristoneura fumiferana (Clem.), moth, (Cf) was also tested for inhibition activity by observation of the THF-hydrate-crystal-growth habit. Like WfAFP, CfAFP appeared to show adsorption on multiple THF-hydrate-crystal faces. A protein with no antifreeze activity, cytochrome C, was used as a control and it neither changed the morphology of the THF clathrate-hydrate crystals, nor retarded the formation of the hydrate. Preliminary experiments on the inhibition activity of WfAFP on a natural gas hydrate assessed induction time and the amount of propane gas consumed. Similar to the observations for THF, the data indicated that WfAFP inhibited propane-hydrate growth. Taken together, these results support our hypothesis that AFPs can inhibit clathrate-hydrate growth and as well, offer promise for the understanding of the inhibition mechanism. PACS No.: 87.90ty

scholarly journals Pectin as an Extraordinary Natural Kinetic Hydrate Inhibitor

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shurui Xu ◽  
Shuanshi Fan ◽  
Songtian Fang ◽  
Xuemei Lang ◽  
Yanhong Wang ◽  
...  
Keyword(s):  
Growth Rate ◽  
Crystal Growth ◽  
Induction Time ◽  
Methane Hydrate ◽  
Hydrate Formation ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Inhibition Activity ◽  
Hydrate Crystal ◽  
Crystal Growth Inhibition

Abstract Pectin as a novel natural kinetic hydrate inhibitor, expected to be eco-friendly and sufficiently biodegradable, was studied in this paper. The novel crystal growth inhibition (CGI) and standard induction time methods were used to evaluate its effect as hydrate inhibitor. It could successfully inhibit methane hydrate formation at subcooling temperature up to 12.5 °C and dramatically slowed the hydrate crystal growth. The dosage of pectin decreased by 66% and effective time extended 10 times than typical kinetic inhibitor. Besides, its maximum growth rate was no more than 2.0%/h, which was far less than 5.5%/h of growth rate for PVCap at the same dosage. The most prominent feature was that it totally inhibited methane hydrate crystal rapid growth when hydrate crystalline occurred. Moreover, in terms of typical natural inhibitors, the inhibition activity of pectin increased 10.0-fold in induction time and 2.5-fold in subcooling temperature. The extraordinary inhibition activity is closely related to its hydrogen bonding interaction with water molecules and the hydrophilic structure. Finally, the biodegradability and economical efficiency of pectin were also taken into consideration. The results showed the biodegradability improved 75.0% and the cost reduced by more than 73.3% compared to typical commercial kinetic inhibitors.

Characterization of CO2 Storage by Clathrate Hydrate Crystallization

2012 ◽  
Vol 433-440 ◽  
pp. 1260-1264
Author(s):  
Ni Liu ◽  
Xiao Bo Xuan ◽  
Ju Li ◽  
Dao Ping Liu ◽  
Ying Ming Xie ◽  
...  
Keyword(s):  
High Pressure ◽  
Induction Time ◽  
Hydrate Formation ◽  
Clathrate Hydrate ◽  
Small Scale ◽  
Equilibrium Curve ◽  
Supercooling Degree ◽  
And Storage

A potential way of CO2 capture and storage by hydrates is proposed in this paper. The characteristics of hydrate formation is investigated in a small scale dynamic reactor under high pressure conditions from 2-3.5Mpa. Temperature varied between 0.5-4°C. The effect of supercooling degree and overpressure on hydrates formation were discussed. Hydrates formation can be completely finished within 150min and the final pressure-temperature points of system are just on the hydrates equilibrium curve. The induction time increasing with the decreasing of supercooling degree and increase of pressure. It is less than 40min under the experiment conditions.

scholarly journals Kinetic inhibition effect of Type I and III antifreeze proteins on unidirectional tetrahydrofuran hydrate crystal growth

RSC Advances ◽  
2019 ◽  
Vol 9 (20) ◽  
pp. 11530-11537 ◽  
Author(s):  
Michihiro Muraoka ◽  
Michika Ohtake ◽  
Yoshitaka Yamamoto
Keyword(s):  
Crystal Growth ◽  
Antifreeze Proteins ◽  
Clathrate Hydrate ◽  
Type I ◽  
Growth Technique ◽  
Inhibition Effect ◽  
Type Iii ◽  
Kinetic Inhibition ◽  
Hydrate Crystal

Experiments were performed to evaluate the kinetic inhibition effect of Type I and Type III antifreeze proteins (AFPs) on the growth of tetrahydrofuran (THF) clathrate hydrate crystals using the unidirectional growth technique.

Why ice-binding type I antifreeze protein acts as a gas hydrate crystal inhibitor

2015 ◽  
Vol 17 (15) ◽  
pp. 9984-9990 ◽  
Author(s):  
S. Alireza Bagherzadeh ◽  
Saman Alavi ◽  
John A. Ripmeester ◽  
Peter Englezos
Keyword(s):  
Gas Hydrate ◽  
Antifreeze Protein ◽  
Winter Flounder ◽  
Methyl Groups ◽  
Type I ◽  
Cooperative Action ◽  
Ice Binding ◽  
Hydrate Crystal ◽  
Binding Type

The winter flounder antifreeze protein (wf-AFP) acts as a gas hydrate crystal inhibitor by binding to the empty-half cages at the hydrate surfaceviathe cooperative action between methyl groups of threonine and alanine residues.

Jump in the Rotational Mobility of Benzene Induced by the Clathrate Hydrate Formation

1995 ◽  
Vol 99 (5) ◽  
pp. 1377-1379 ◽  
Author(s):  
Masaru Nakahara ◽  
Chihiro Wakai ◽  
Nobuyuki Matubayasi
Keyword(s):  
Hydrate Formation ◽  
Clathrate Hydrate ◽  
Rotational Mobility

scholarly journals Valonea Tannin: Tyrosinase Inhibition Activity, Structural Elucidation and Insights into the Inhibition Mechanism

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2747
Author(s):  
Jiaman Liu ◽  
Yuqing Liu ◽  
Xiaofeng He ◽  
Bo Teng ◽  
Jacqui M. McRae
Keyword(s):  
Molecular Weight ◽  
Structure Function ◽  
Hydrophobic Interactions ◽  
Average Molecular Weight ◽  
Commercial Application ◽  
Inhibition Mechanism ◽  
Resonance Spectroscopy ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Function Activity

Valonea tannin is a natural product readily extracted from acorn shells that has been suggested to have potential skin whitening properties. This study investigated the tyrosinase inhibition activity of extracted valonea tannin and the associated structure–function activity. Nuclear magnetic resonance spectroscopy and molecular weight analysis with gel permeation chromatography revealed that valonea tannin could be characterized as a hydrolysable tannin with galloyl, hexahydroxydiphenoyl and open formed-glucose moieties and an average molecular weight of 3042 ± 15 Da. Tyrosinase inhibition assays demonstrated that valonea tannin was 334 times more effective than gallic acid and 3.4 times more effective than tannic acid, which may relate to the larger molecular size. Kinetic studies of the inhibition reactions indicated that valonea tannin provided tyrosinase inhibition through mixed competitive–uncompetitive way. Stern–Volmer fitted fluorescence quenching analysis, isothermal titration calorimetry analysis and in silico molecule docking showed valonea tannin non-selectively bound to the surface of tyrosinase via hydrogen bonds and hydrophobic interactions. Inductively coupled plasma-optical emission spectroscopy and free radical scavenging assays indicated the valonea tannin had copper ion chelating and antioxidant ability, which may also contribute to inhibition activity. These results demonstrated the structure–function activity of valonea tannin as a highly effective natural tyrosinase inhibitor that may have commercial application in dermatological medicines or cosmetic products.

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