Possible involvement ofPseudomonas fluorescensand Bacillaceae in structural modifications ofTuber borchiifruit bodies

2001 ◽  
Vol 47 (3) ◽  
pp. 264-268 ◽  
Author(s):  
Barbara Citterio ◽  
Manuela Malatesta ◽  
Serafina Battistelli ◽  
Francesco Marcheggiani ◽  
Wally Baffone ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Population ◽  
Cellulolytic Bacteria ◽  
Tuber Borchii ◽  
Structural Modifications ◽  
Early Maturation ◽  
Intimate Association ◽  
Maturation Stages ◽  
Decomposition Stages

Previous studies on Tuber borchii fruit bodies in early maturation stages suggested a role of bacteria in sporocarp structural modifications. In order to verify this hypothesis, in the present study we investigated by means of microbial and ultrastructural approaches, the bacterial population of T. borchii sporocarps from intermediate maturation phases to advanced decomposition stages, paying particular attention to chitinolytic and cellulolytic bacteria and to their relationships with ascii and ascospores. We found that Pseudomonas fluorescens and spore-forming Bacillaceae, both able to degrade cellulose and chitin, are present inside the sporocarps in all maturation stages investigated. Moreover, rod-shaped bacteria seem able to erode ascus walls and colonize the interior of ascii containing mature spores. These results suggest a possible role of these bacteria in the process of ascus opening. Moreover, the presence of P. fluorescens and Bacillaceae on isolated mature spores after decontamination suggests an intimate association between these bacteria and the ascospores.Key words: bacteria, cellulose, chitin, ectomycorrhiza.

Possible involvement of Pseudomonas fluorescens and Bacillaceae in structural modifications of Tuber borchii fruit bodies

2001 ◽  
Vol 47 (3) ◽  
pp. 264-268 ◽  
Author(s):  
Barbara Citterio ◽  
Manuela Malatesta ◽  
Serafina Battistelli ◽  
Francesco Marcheggiani ◽  
Wally Baffone ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Tuber Borchii ◽  
Structural Modifications

scholarly journals Exploring Secondary Metabolite Profiles of Stachybotrys spp. by LC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 133 ◽  
Author(s):  
Annika Jagels ◽  
Viktoria Lindemann ◽  
Sebastian Ulrich ◽  
Christoph Gottschalk ◽  
Benedikt Cramer ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Future Research ◽  
Structural Modifications ◽  
Metabolite Profiles ◽  
The Individual ◽  
First Time ◽  
Analytical Standards ◽  
Respective Species

The genus Stachybotrys produces a broad diversity of secondary metabolites, including macrocyclic trichothecenes, atranones, and phenylspirodrimanes. Although the class of the phenylspirodrimanes is the major one and consists of a multitude of metabolites bearing various structural modifications, few investigations have been carried out. Thus, the presented study deals with the quantitative determination of several secondary metabolites produced by distinct Stachybotrys species for comparison of their metabolite profiles. For that purpose, 15 of the primarily produced secondary metabolites were isolated from fungal cultures and structurally characterized in order to be used as analytical standards for the development of an LC-MS/MS multimethod. The developed method was applied to the analysis of micro-scale extracts from 5 different Stachybotrys strains, which were cultured on different media. In that process, spontaneous dialdehyde/lactone isomerization was observed for some of the isolated secondary metabolites, and novel stachybotrychromenes were quantitatively investigated for the first time. The metabolite profiles of Stachybotrys species are considerably influenced by time of growth and substrate availability, as well as the individual biosynthetic potential of the respective species. Regarding the reported adverse effects associated with Stachybotrys growth in building environments, combinatory effects of the investigated secondary metabolites should be addressed and the role of the phenylspirodrimanes re-evaluated in future research.

The role of the glyoxylate cycle in the symbiotic fungus Tuber borchii: expression analysis and subcellular localization

2007 ◽  
Vol 52 (3-4) ◽  
pp. 159-170 ◽  
Author(s):  
Simona Abba’ ◽  
Raffaella Balestrini ◽  
Alessandra Benedetto ◽  
Hanspeter Rottensteiner ◽  
José Ramón De Lucas ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Expression Analysis ◽  
Glyoxylate Cycle ◽  
Tuber Borchii ◽  
Symbiotic Fungus ◽  
The Glyoxylate Cycle

scholarly journals Cytochemical localization of calcium and Ca2+,Mg2(+)-adenosine triphosphatase in colchicine-altered rat incisor ameloblasts.

1990 ◽  
Vol 38 (10) ◽  
pp. 1469-1478 ◽  
Author(s):  
D R Eisenmann ◽  
A H Salama ◽  
A M Zaki ◽  
S H Ashrafi
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Adenosine Triphosphatase ◽  
Morphological Changes ◽  
Rat Incisor ◽  
Cytochemical Localization ◽  
Early Maturation ◽  
Transmission Electron ◽  
Maturation Stages ◽  
Secretory Transport

Colchicine is known to affect secretory, transport, and degradative functions of ameloblasts. The effects of colchicine on membrane-associated calcium and Ca2+,Mg2(+)-ATPase in secretory and maturation ameloblasts were investigated cytochemically. The pyroantimonate (PPA) method was used for localizing calcium and a modified Wachstein-Meisel medium was used to localize Ca2+,Mg2(+)-ATPase. Sections representing secretory and early maturation stages were examined by transmission electron microscopy. Morphological changes induced by colchicine included dislocated organelles and other well-established reactions to such anti-microtubule drugs. Calcium pyroantimonate (Ca-PA) deposits in most ameloblast types were markedly reduced, with the greater reduction occurring in those cells more severely altered morphologically. However, the cell membranes of both control and experimental smooth-ended maturation ameloblasts were essentially devoid of Ca-PA. The normal distribution and intensity of Ca2+,Mg2(+)-ATPase was not affected by colchicine. Because the observed reduction of membrane-associated calcium is apparently not mediated by Ca2+,Mg2(+)-ATPase in this case, other aspects of the calcium regulating system of ameloblasts are apparently targeted by colchicine.

Neural induction and the structure of the target cell surface

Development ◽  
1982 ◽  
Vol 70 (1) ◽  
pp. 171-187
Author(s):  
A. M. Duprat ◽  
L. Gualandris ◽  
P. Rouge
Keyword(s):  
Cell Surface ◽  
Structural Integrity ◽  
Neural Induction ◽  
Active Role ◽  
Target Cells ◽  
Similar Treatment ◽  
Structural Modifications ◽  
Pleurodeles Waltl

Lectins (SBA and PSA) were used to provoke crowding and structural modifications of the presumptive ectoderm cell surface in order to investigate the role of the membrane organization of the competent target cells in neural induction. Are specific characteristics of the cell surface essential for this phenomenon to occur? From amphibian gastrulae, it is possible to obtain neural induction in vitro by association of presumptive ectoderm (target cells) with chordamesoderm (inductor tissue): 4 h of contact is sufficient in Pleurodeles waltl for transmission of the inductive signal. Very quickly, the treatment of the normal ectoderm by lectins (SBA-FITC or PSA-FITC) provoked surface modifications. Lectin-treatment (50 µg ml1−, 30 min) of presumptive ectoderm did not result in any neural induction. Lectin-treatment (50 µg ml1−, 30 min) of presumptive ectoderm previous to its association with the natural inductor for 4 h, disturbed the phenomenon: no induction. Similar treatment followed by association with the inductor for 24 h: induction. Treatment of SBA or PSA with their respective hapten inhibitors prior to addition to ectodermal cells completely blocked the suppressive effects on induction. The structural integrity of the membrane of competent target cells is necessary for neural induction to occur. The cell membrane could thus play, directly or indirectly, an active role in the specificity of this process

scholarly journals Role of endomycorrhizae and Pseudomonas fluorescens on the acclimatization of micropropagated Stevia rebaudiana Bert. plantlets

2017 ◽  
Vol 11 (3) ◽  
pp. 38-47
Author(s):  
S. Zayed Mona ◽  
Abd El-Moneim Hegazi Ghada ◽  
Mohammed Salem Hanaa ◽  
Mohammed Ibrahim Ali Adas Walaa
Keyword(s):  
Pseudomonas Fluorescens ◽  
Stevia Rebaudiana

scholarly journals Efficacy of Clarithromycin Depends on the Bacterial Density in Clarithromycin-Heteroresistant Helicobacter pylori Infections: An In Situ Detected Susceptibility and Quantitative Morphometry-Based Retrospective Study

2021 ◽  
Vol 27 ◽  
Author(s):  
Jewel Ju Ea Kim ◽  
Ildikó Kocsmár ◽  
György Miklós Buzás ◽  
Ildikó Szirtes ◽  
Orsolya Rusz ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Retrospective Study ◽  
Bacterial Population ◽  
Tissue Sample ◽  
Bacterial Density ◽  
Resistant Bacteria ◽  
Hybridization Technique ◽  
Predictive Role

The global rise in clarithromycin (Cla) resistance is considered to be the main contributor of Helicobacter pylori (Hp) eradication failures. In nearly half of the Cla-resistant Hp infections, Cla-susceptible bacteria are simultaneously present with the Cla-resistant ones (Cla-heteroresistance). The proportion of resistant bacteria in the bacterial population (R-fraction) and its predictive role for the use of Cla-based therapies in Cla-heteroresistant infections has not yet been investigated. Our retrospective study analyzed gastric biopsy samples of 62 Hp-positive patients with Cla-heteroresistant infection. Fluorescence In Situ Hybridization technique was used to visualize the coexistence of resistant and susceptible bacteria within one tissue sample. R-fraction was quantified on multichannel microimages by digital morphometry. Resistant bacteria had a patchy distribution within the whole bacterial population causing high diversity among the investigated areas. Patients were subdivided into two major groups according to whether a Cla-based eradication attempt was conducted before or after the biopsy sampling. R-fraction was significantly lower among cases having only one previous Cla-based eradication attempt vs. those that had multiple previous eradications, including at least one Cla-containing therapy (0.41 vs. 0.89, p = 0.0308). Majority of the patients without previous eradication attempt had successful eradication with Cla-containing regimen (59.26%), verified by a negative 13C-urea breath test or control biopsy. Multivariable model indicated that the therapeutic outcome using Cla-based regimens depended on the bacterial density rather than the R-fraction. Our study raises the potential use of Cla-containing eradication therapies in certain Cla-heteroresistant Hp infections, taking into account the possible predictive role of bacterial density.

scholarly journals Evaluation of extracellular enzyme-producing autochthonous gut bacteria in walking catfish, Clarias batrachus (L.)

2016 ◽  
Vol 4 (1) ◽  
pp. 345 ◽  
Author(s):  
Atrayee Dey ◽  
Koushik Ghosh ◽  
Niladri Hazra
Keyword(s):  
Enzyme Assay ◽  
Bacterial Population ◽  
Gene Sequence ◽  
Extracellular Enzyme ◽  
Gut Bacteria ◽  
Clarias Batrachus ◽  
Cellulolytic Bacteria ◽  
Qualitative Assay ◽  
Overall Health Status

The present study was carried out to screen autochthonous gut bacteria in freshwater air breathing walking catfish, Clarias batrachus Linnaeus. Altogether, 100 extracellular enzyme-producing bacteria were isolated from the foregut (FG) and hindgut (HG) regions. Data were presented as log viable counts g-1 gut (LVC). The occurrence of heterotrophic bacterial population was higher in the FG region (LVC = 8.25) than the HG (LVC= 7.3). Similarly, proteolytic, amylolytic and lipolytic bacteria in FG outnumbered (LVC=7.25, 6.77 and 5.23 respectively) the HG (LVC=6.38, 5.58 and 4.04 respectively). However, occurrence of cellulolytic bacteria in both, FG and HG was less (LVC=2.1 and 1.34 respectively) in comparison to the other extracellular enzyme-producing bacteria. Out of the 100 bacterial isolates, 22 isolates were primarily selected through qualitative assay of extracellular enzyme activities. Among them, 3 promising isolates were chosen as potent extracellular enzyme producers on the basis of cumulative scores (≥11) of the qualitative assay and quantitative enzyme assay. Maximum protease activity was revealed by the strain FG10 (201±2.40U), while FG43 exhibited maximum amylase (208.3±10.8U) and lipase (4.73±0.05U) activities. Among the strains isolated from the HG, the highest protease (188.3±1.2U), amylase (97.6±0.46U) and lipase (3.7±0.11U) activities were recorded with the strain HG01. The isolates (FG10, FG43 and HG01) were studied through 16S rRNA partial gene sequence analyses and were identified as Bacillus aryabhattai (KP784311), B. flexus (KR809411), and B. cereus (KR809412), respectively. Further studies are to be conducted to evaluate the efficacy of these strains in vivo to improve the overall health status of the C. batrachus juveniles.

scholarly journals Metabolic activity of gut microbiota and xenobiotics

2015 ◽  
pp. 47-55 ◽  
Author(s):  
Gordana Bojic ◽  
Svetlana Golocorbin-Kohn ◽  
Maja Stojancevic ◽  
Momir Mikov ◽  
Ljiljana Suvajdzic
Keyword(s):  
Gut Microbiota ◽  
Human Health ◽  
Metabolic Activity ◽  
Bacterial Population ◽  
Great Benefit ◽  
Host Organism ◽  
Symbiotic Microorganisms ◽  
Metabolic Activities ◽  
Bacterial Genes

The intestine habitat is the natural collection of symbiotic microorganisms. The bacterial population enables many permanent metabolic activities in this environment. Inside the intestine of mammals there are an extended genome of millions of bacterial genes named microbiome. In recent years, there has been an increased interest of scientists to discover the place and the role of bio-ecological content and modulation of gut microbiota in a host organism using prebiotics, probiotics and synbiotics, which may have a great benefit for human health.

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