Ralstonia basilensisM91-3, a denitrifying soil bacterium capable of usings-triazines as nitrogen sources

The purpose of this study was to characterize the phylogenetic and phenotypic traits of M91-3, a soil bacterium capable of mineralizing atrazine (2-chloro-4-N-isopropyl-6-N-ethyl-s-triazine). The isolate was identified as Ralstonia basilensis based on 99.5% homology of the 16S rRNA sequence and various chemotaxonomic data. The isolate used atrazine as the sole source of energy, carbon, and nitrogen. It could also use several other s-triazines as nitrogen sources. Ralstonia basilensis M91-3 was capable of denitrification, which was confirmed by gas chromatographic analysis of nitrous oxide under acetylene blockage conditions.Key words: atrazine biodegradation, denitrification, herbicide degrader, Ralstonia basilensis, triazine degradation.

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Lab-Scale Optimization of Polyhydroxyalkanoate Production by Bacterial Strain cmg1415 on Local Cheap Substrates Using One Variable at a Time Approach

Journal of Plant and Environment ◽

10.33687/jpe.001.01.3577 ◽

2021 ◽

Vol 1 (1) ◽

pp. 25-31

Author(s):

Muhammadi Muhammadi ◽

Shabina Shafiq

Keyword(s):

Sugar Beet ◽

Minimal Medium ◽

Maximum Yield ◽

Nitrogen Sources ◽

Sole Source ◽

Labor Cost ◽

Culture Conditions ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Scale Optimization

Production of polyhydroxyalkanoate (PHA) under optimum culture conditions using local cheap feedstocks is indispensable to overcome the current cost of PHA-based plastics. For this purpose, optimum culture conditions and cheap feedstocks were investigated to produce maximum yield of PHA in CMG1415. Maximum yield was obtained with sucrose or sugar beet as sole source of precursors for PHA in 8 days of incubation at 35 °C in a minimal medium adjusted at pH 7. Further, for maximum yield no mechanical shaking was needed. Local cheap feedstock such as sugar beet and molasses were found to play as significant carbon and nitrogen sources for maximum PHA yield. Bacterial plastic produced under these low-labor-cost culture conditions may to reduce the present cost of degradable bioplastic and be much effective alternate of nondegradable varieties of synthetic plastic.

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Optimization of Keratinase Production By Actinomyces Fradiae 119 and Its Application in Degradation of Keratin Containing Wastes

Environment Technology Resources Proceedings of the International Scientific and Practical Conference ◽

10.17770/etr2011vol1.905 ◽

2015 ◽

Vol 1 ◽

pp. 294

Author(s):

V. Matikevičienė ◽

S. Grigiškis ◽

D. Levišauskas ◽

K. Sirvydytė ◽

O. Dižavičienė ◽

...

Keyword(s):

Basal Medium ◽

Nitrogen Sources ◽

Sole Source ◽

Optimal Parameters ◽

Technological Parameters ◽

Keratinolytic Activity ◽

Carbon And Nitrogen ◽

Chicken Feathers ◽

Good Ability ◽

Cultivation Process

The aim of this study was to identify and optimize significant technological parameters influencing keratinolytic enzyme production by A. fradiae 119 and to study its ability to degrade keratin. In the present work chicken feathers meal (CFM) was found to be an excellent substrate for keratinase induction by A. fradiae 119. The strain produced 164 KU/mL keratinolytic activity in basal medium containing 7.5 g/L CFM as the sole source of carbon and nitrogen. Increased keratinolytic activity was achieved in media with ammonium sulfate as nitrogen source, the application of additional nitrogen sources to media containing CFM slightly decreased keratinase synthesis. Optimal parameters of the cultivation process were determined: pH of cultivation medium – 7.2, temperature – 34 ºC and inoculum’s size – 8 %, using the response surface methodology. The yield of keratinase activity was increased by 46 % (267 KU/mL) after optimization of the cultivation process. The good ability of cultural liquid to degrade feathers and wool was detected.

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Photoinduced perithecial formation by Nectria haematococca on media containing either L-tyrosine; L-phenylalanine; or D-glucose + NaNO3 as sole carbon and nitrogen sources

Canadian Journal of Microbiology ◽

10.1139/m69-154 ◽

1969 ◽

Vol 15 (8) ◽

pp. 863-868 ◽

Cited By ~ 1

Author(s):

C. R. Curtis

Keyword(s):

Basal Medium ◽

Nitrogen Sources ◽

Sole Source ◽

Carbon And Nitrogen Sources ◽

Light Requirement ◽

Carbon And Nitrogen ◽

Significant Difference ◽

Tyrosine Metabolism ◽

Initial Ph ◽

Relationship Of

A homothallic isolate of Fusarium solani produced red perithecia when illuminated and grown on a simple basal medium containing L-tyrosine; L-phenylalanine; or D-glucose + NaNO3 as a sole carbon and nitrogen source. Few or no perithecia formed in darkness on these media. Quantitative comparisons of perithecial formation in illuminated treatments indicated that L-tyrosine was a significantly better sole source of carbon and nitrogen than either L-phenylalanine or D-glucose + NaNO3. There was no significant difference between the responses on media containing L-phenylalanine and D-glucose + NaNO3. If dark-grown treatments were subsequently illuminated, perithecia formed on media containing L-tyrosine and D-glucose + NaNO3 but not on L-phenylalanine. The initial pH of the medium containing L-tyrosine did not seem to affect the fruiting response in illuminated cultures. A reduction in the amount of L-tyrosine in the basal medium resulted in a corresponding decrease in perithecial formation.The results are discussed in connection with a possible relationship of sexual reproduction, the light requirement, and tyrosine metabolism.

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Mineralization of the Cyclic Nitramine Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine by Gordonia and Williamsia spp

Applied and Environmental Microbiology ◽

10.1128/aem.71.12.8265-8272.2005 ◽

2005 ◽

Vol 71 (12) ◽

pp. 8265-8272 ◽

Cited By ~ 106

Author(s):

Karen T. Thompson ◽

Fiona H. Crocker ◽

Herbert L. Fredrickson

Keyword(s):

Nitrogen Source ◽

Surface Soil ◽

Nitrogen Sources ◽

Sole Source ◽

Ribosomal Gene ◽

Aerobic Bacteria ◽

Fatty Acid Profiles ◽

Bacterial Strains ◽

Carbon And Nitrogen ◽

Pure Bacterial Cultures

ABSTRACT Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a cyclic nitroamine explosive that is a major component in many military high-explosive formulations. In this study, two aerobic bacteria that are capable of using RDX as the sole source of carbon and nitrogen to support their growth were isolated from surface soil. These bacterial strains were identified by their fatty acid profiles and 16S ribosomal gene sequences as Williamsia sp. KTR4 and Gordonia sp. KTR9. The physiology of each strain was characterized with respect to the rates of RDX degradation and [U-14C]RDX mineralization when RDX was supplied as a sole carbon and nitrogen source in the presence and absence of competing carbon and nitrogen sources. Strains KTR4 and KTR9 degraded 180 μM RDX within 72 h when RDX served as the only added carbon and nitrogen source while growing to total protein concentrations of 18.6 and 16.5 μg/ml, respectively. Mineralization of [U-14C]RDX to 14CO2 was 30% by strain KTR4 and 27% by KTR9 when RDX was the only added source of carbon and nitrogen. The addition of (NH4)2SO4 greatly inhibited KTR9's degradation of RDX but had little effect on that of KTR4. These are the first two pure bacterial cultures isolated that are able to use RDX as a sole carbon and nitrogen source. These two genera possess different physiologies with respect to RDX mineralization, and each can serve as a useful microbiological model for the study of RDX biodegradation with regard to physiology, biochemistry, and genetics.

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THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS

Journal of Critical Reviews ◽

10.31838/jcr.07.04.94 ◽

2020 ◽

Vol 7 (04) ◽

Keyword(s):

Ethanol Production ◽

Nitrogen Sources ◽

Optimal Conditions ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen

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A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽

10.1186/s13568-020-01118-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Yanhua Yao ◽

Guimei Zhou ◽

Yonghui Lin ◽

Xinqi Xu ◽

Jie Yang

Keyword(s):

Malachite Green ◽

Fermentation Broth ◽

Nitrogen Sources ◽

Life Time ◽

Industrial Applications ◽

Dynamics Simulation ◽

Carbon And Nitrogen ◽

Cdna Sequences ◽

Cerrena Unicolor ◽

Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

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Pseudomonas eucalypticola sp. nov., a producer of antifungal agents isolated from Eucalyptus dunnii leaves

Scientific Reports ◽

10.1038/s41598-021-82682-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yujing Liu ◽

Zhang Song ◽

Hualong Zeng ◽

Meng Lu ◽

Weiyao Zhu ◽

...

Keyword(s):

Dna Hybridization ◽

Antifungal Agents ◽

Novel Species ◽

Phytopathogenic Fungi ◽

Strictly Aerobic ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Eucalyptus Dunnii ◽

16S Rrna Sequence Analysis

AbstractPseudomonas are ubiquitously occurring microorganisms and are known for their ability to produce antimicrobials. An endophytic bacterial strain NP-1 T, isolated from Eucalyptus dunnii leaves, exhibits antifungal properties against five tested phytopathogenic fungi. The strain is a Gram-negative rod-shaped bacterium containing a single polar flagellum. It is strictly aerobic, grows at 4–37 °C, 2–5% NaCl, and pH 3–7. The 16S rRNA sequence analysis showed that NP-1 T belongs to the Pseudomonas genus. Phylogenetic analysis based on four concatenated partial genes (16S rDNA, gyrB, rpoB and rpoD) and the phylogenomic tree indicated that NP-1 T belongs to Pseudomonas fluorescens lineage but is distinct from any known Pseudomonas species. The G + C mol % of NP-1 T genome is 63.96, and the differences between NP-1 T and related species are larger than 1. The digital DNA-DNA hybridization and tetranucleotide signatures are 23.8 and 0.97, which clearly separates strain NP-1 T from its closest neighbours, Pseudomonas coleopterorum and Pseudomonas rhizosphaerae. Its phenotypic and chemotaxonomic features confirmed its differentiation from related taxa. The results from this polyphasic approach support the classification of NP-1 T as a novel species of Pseudomonas, and the name of Pseudomonas eucalypticola is thus proposed for this strain, whose type is NP-1 T (= CCTCC M2018494T = JCM 33572 T).

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