Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of the genus Herbaspirillum

2010 ◽  
Vol 56 (4) ◽  
pp. 342-347 ◽  
Author(s):  
R. V. Serrato ◽  
G. L. Sassaki ◽  
L. M. Cruz ◽  
R. W. Carlson ◽  
A. Muszyński ◽  
...  

Bacteria from the genus Herbaspirillum are endophytes responsible for nitrogen fixation in gramineous plants of economic importance such as maize, sugarcane, sorghum, rice, and wheat. Some species are known to produce plant growth substances. In contrast, Herbaspirillum rubrisubalbicans strains are known to be mild plant pathogens. The molecular communication between the plant and the microbes might involve lipopolysaccharides present in the outer membrane of these gram-negative bacteria. Phenol–water extraction was used to obtain lipopolysaccharides from 7 strains of Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95, and M2) and H. rubrisubalbicans (M1 and M4). The electrophoretic profiles and chemical composition of the lipopolysaccharides obtained in the phenol and aqueous extracts were shown herein.

2010 ◽  
Vol 5 (9) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Juergen Wanner ◽  
Erich Schmidt ◽  
Stefanie Bail ◽  
Leopold Jirovetz ◽  
Gerhard Buchbauer ◽  
...  

The chemical composition of essential oils of cabreuva ( Myrocarpus Fastigiatus Allemao, Fabaceae) from Brazil, cedarwood ( Juniperus ashei, Cupressaceae) from Texas, Juniper berries ( Juniperus communis L., Cupressaceae) and myrrh ( Commiphora myrrha (Nees) Engl., Burseraceae) were analyzed using GC/FID and GC/MS. The antimicrobial activity of these essential oils and some of their main compounds were tested against eleven different strains of Gram-positive and Gram-negative bacteria by using agar diffusion and agar serial dilution methods. Animal and plant pathogens, food poisoning and spoilage bacteria were selected. The volatile oils exhibited considerable inhibitory effects against all tested organisms, except Pseudomonas, using both test methods. Higher activity was observed against Gram-positive strains in comparison with Gram-negative bacteria. Cabreuva oil from Brazil showed similar results, but in comparison with the other oils tested, only when higher concentrations of oil were used.


2021 ◽  
pp. 47-56
Author(s):  
Oki Puspita Wardani ◽  
Priyadi Priyadi ◽  
Yatmin Yatmin

This study was conducted on Pujodadi Village, Trimurjo, Central Lampung from September to December 2020. The study used a factorial randomized block design with two factors and three replications. The first factor is concentration of plant growth substances which consists of 5 levels; Z0 (0 ml.lt-1), Z1 (3.5 ml.lt-1), Z2 (7 ml.lt-1), Z3 (10.5 ml.lt-1) and Z4 (14 ml.lt-1). The second factor is the origin of the seed consists of three parts; P1 (top), P2 (middle), and P3 (bottom). The research objective was to study the effect of plant growth substances concentration and part of seed origin in sugarcane seedlings. The results showed that the plant growth substances concentration significantly increased the percentage of growth and the time 50% of the seeds sprouted. The seed origin of the top part showed the best growth compared to the middle and bottom. There was no interaction between plant growth substances concentration and seed origin on sugarcane seed growth.


2015 ◽  
Vol 39 (6) ◽  
pp. 1595-1602 ◽  
Author(s):  
Valfredo Almeida Chaves ◽  
Silvana Gomes dos Santos ◽  
Nivaldo Schultz ◽  
Willian Pereira ◽  
Jailson Silva Sousa ◽  
...  

RESUMO A cana-de-açúcar é uma cultura de grande destaque na economia, em razão da produção de açúcar, etanol e energia. Tecnologias que possam contribuir para o aumento da produtividade e qualidade da cultura com mínimos danos ao meio ambiente são necessárias. Objetivou-se avaliar a produção de ácido indol acético de cinco estirpes de bactérias diazotróficas e o efeito da inoculação delas na brotação de duas variedades de cana-de-açúcar, RB867515 e IACSP95-5000. A produção de auxina foi determinada pelo teste colorimétrico, usando o reagente de Salkowski. Para avaliar a germinação, foi conduzido um experimento em casa de vegetação, utilizando-se delineamento experimental em blocos ao acaso com quatro repetições e sete tratamentos: controle não inoculado; inoculação mista com as cinco estirpes e inoculação individual com Gluconacetobacter diazotrophicus (Gd) estirpe BR11281T(PAL-5T), Herbaspirillum seropedicae (Hs - BR11335 = HRC54), Herbaspirillum rubrisubalbicans (Hr - BR11504 = HCC103), Burkholderia tropica (Bt - BR11366T = PPe 8 T) e Azospirillum amazonense (Aa - BR11145 = CBAMc). As bactérias mais eficientes na produção de auxina foram Hs e Hr, declinando 48 h após o crescimento. Hr, Aa e Bt aumentaram o índice de velocidade de germinação e o número de brotações nas duas variedades. Na var. RB867515, a velocidade de germinação ainda foi positivamente influenciada pela inoculação mista, sendo o mesmo observado pela inoculação de Gd na var. IACSP95-5000.


Author(s):  
Zhiguo Ju ◽  
Lixiao Ding ◽  
Kangjun Li ◽  
Zhen Ding ◽  
Qingna Zhang ◽  
...  

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