In vitro micropropagation of Afghan pine

1994 ◽  
Vol 24 (6) ◽  
pp. 1248-1252 ◽  
Author(s):  
S. Sen ◽  
M.E. Magallanes-Cedeno ◽  
R.H. Kamps ◽  
C.R. McKinley ◽  
R.J. Newton
Keyword(s):  
Clonal Propagation ◽  
Christmas Tree ◽  
Naphthalene Acetic Acid ◽  
Root Primordia ◽  
Indole Butyric Acid ◽  
Cotyledon Explants ◽  
In Vitro Micropropagation ◽  
Shoot Production ◽  
Tree Production

Clonal propagation by tissue culture of Afghan pine (Pinuseldarica Medw.) could be useful for improving Christmas tree production and afforestation of semiarid lands in North America. The objectives were (i) to develop organogenesis procedures for clonal propagation and (ii) to evaluate plantlet performance in the field. Shoot production from cotyledon explants was accomplished on solid Shenk and Hildebrandt medium amended with kinetin (11 μM) and benzyladenine (11 μM). Over 100 shoots per clone were obtained with this method. Spontaneous rooting was prevalent in some of the induced shoots. Shoots without root primordia were induced to form roots with naphthalene acetic acid (5 μM), indole butyric acid (10 μM), and benzyladenine (0.22 μM) in 1/2 strength Shenk and Hildebrandt medium. The rate of plantlet elongation in the field was similar to that of seedlings; however, survival of plantlets was lower than that of seedlings. The lower survival of plantlets was directly related to their smaller size at the time of planting. Organogenesis appears to be a very suitable method for mass micropropagation of this environmentally and economically useful tree.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Development of a robust method for propagation of Leptospermum scoparium J.R.Forst. & G.Forst. from semi-hardwood cuttings

2020 ◽  
Author(s):  
Tate Jason James Hancox ◽  
Rachel A Burton ◽  
Kate Louise Delaporte
Keyword(s):  
Clonal Propagation ◽  
Natural Populations ◽  
Naphthalene Acetic Acid ◽  
Maturity Stage ◽  
Root Induction ◽  
Indole Butyric Acid ◽  
Transplant Survival ◽  
Detailed Method ◽  
Significant Difference ◽  
Leptospermum Scoparium

Abstract BackgroundLeptospermum scoparium J.R.Forst. & G.Forst. is highly valued for the production of antimicrobial honey. Both researchers and the Australian honey industry are interested in the clonal propagation of these plants to be used for experiments and plantation establishment. However, little information is available on clonal propagation by cuttings. Here we sought to develop a detailed method for L. scoparium propagation by semi-hardwood cuttings by testing the influence of genotype and auxin (indole butyric acid (IBA) and IBA + naphthalene acetic acid (NAA)) concentration on plants originating from four natural populations in Victoria, Australia. ResultsThere was no significant difference in root induction, which ranged from 50.9 – 68.0%, in the four clonal source plants selected. The application of 3 g/L IBA increased root induction from 46.6% of the control cuttings to 77.6% of treated cuttings and stimulated longer roots. The application of 8 g/L IBA increased leaf fall, the presence of necrosis, from 22.5% on the controls to 82.8% on dipped cuttings, and cutting death.Although there was no significant difference in root induction, cuttings that were ‘watered-in’ after planting dropped significantly fewer leaves and had significantly lower rates of necrosis than cuttings watered in after 2-3 days. It was also found that the maturity stage of semi-hardwood cuttings did not significantly affect any of the variables measured. Transplant survival ranged from 78.1 – 89.0% for cuttings treated with auxin and 77.3 to 92.2% for the four clonal source plants. ConclusionThis study found that the clonal source plants selected did not affect the success of propagation. Overall for maximum success, semi-hardwood cuttings can be collected from the current or previous seasons growth, be treated with 3 g/L IBA gel, ‘watered in’ and placed into a propagation tent for approximately six weeks. Cuttings that form roots will transplant easily with high transplant survival.

scholarly journals Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Saccharum Officinarum ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

scholarly journals In vitro Propagation and Plantlet Regeneration from Doritaenopsis Purple Gem ‘Ching Hua’ Flower Explants

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1256-1258 ◽  
Author(s):  
Wagner A. Vendrame ◽  
Ian Maguire ◽  
Virginia S. Carvalho
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
Flower Buds ◽  
Flower Stem ◽  
Normal Flower ◽  
Regenerated Plantlets ◽  
Lateral Flower

The effects of four types of explants removed from 10-cm flower stalks of Doritaenopsis Purple Gem ‘Ching Hua’ (immature apical flower buds, immature lateral flower buds, flower stem nodes, and flower pedicel sections) and combinations of two plant growth regulators [naphthalene acetic acid (NAA) and thidiazuron (TDZ)] on direct in-vitro shoot induction and multiplication were studied. Immature apical flower buds were the only explants that showed induction and multiplication of shoots in vitro. NAA at 5.4 and 10.7 μm combined with either 4.5 or 9.1 μm TDZ provided the fastest and greatest percentages of shoot induction (27% to 40%) and the greatest numbers of shoot multiplication (111–160 shoots per explant). In vitro–induced shoots were rooted on medium containing 5.4 μm NAA and developed into plantlets with normal vegetative and reproductive morphology. Regenerated plantlets were acclimatized, showing 100% survival and establishment in greenhouse. Plantlets were grown to maturity and showed normal flower morphology. No floral off-types were observed. The high rates of shoot multiplication obtained offer a means for mass clonal propagation of this and possibly other related Doritaenopsis hybrids.

scholarly journals A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

2020 ◽  
Vol 36 ◽  
pp. 66-74
Author(s):  
Mohammad Javad Mahmoudi Meimand ◽  
Mohammad Hossein Shamshiri ◽  
Khalil Malekzadeh ◽  
Mohammad Reza Dehghani
Keyword(s):  
Sucrose Concentration ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
The Media ◽  
Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efficiency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

scholarly journals COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS

2007 ◽  
Vol 44 (07) ◽  
pp. 514-519 ◽  
Author(s):  
Ved Prakash Pandey ◽  
Jose Kudakasseril ◽  
Elizabeth Cherian ◽  
George Patani ◽  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Multiplication ◽  
Rauwolfia Serpentina ◽  
In Vitro Micropropagation

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Ilse-Yazmín Arciniega-Carreón ◽  
Carmen Oliver-Salvador ◽  
María-Guadalupe Ramírez-Sotelo ◽  
Carlos Edmundo Salas
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Medicinal Plant ◽  
Metabolic Disorders ◽  
Naphthalene Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Shoot Production ◽  
In Vitro Plant ◽  
Indole 3 Acetic Acid

Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

scholarly journals Chemical Composition and Cytotoxic Activity of the Essential Oil and Oleoresins of In Vitro Micropropagated Ansellia africana Lindl: A Vulnerable Medicinal Orchid of Africa

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4556
Author(s):  
Md. Moshfekus Saleh-E-In ◽  
Paromik Bhattacharyya ◽  
Johannes Van Staden
Keyword(s):  
Essential Oil ◽  
Cytotoxic Activity ◽  
Butyl Acetate ◽  
Herbal Medicines ◽  
Future Research ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Standard Drug

Orchids are rich treasure troves of various important phytomolecules. Among the various medicinal orchids, Ansellia africana stands out prominently in the preparing of various herbal medicines due to its high therapeutic importance. The nodal explants of A. africana were sampled from asymbiotically germinated seedlings on basal Murashige and Skoog (MS) medium and were micropropagated in MS medium supplemented with 3% sucrose and 10 µM meta topolin (mT) + 5 µM naphthalene acetic acid (NAA) +15 µM indole butyric acid (IBA) + 30 µM phloroglucinol (PG). In the present study, the essential oil was extracted by hydrodistillation and the oleoresins by the solvent extraction method from the micropropagated A. africana. The essential oil and the oleoresins were analysed by Gas Chromatography (GC) and GC/MS (Mass spectrometry). A total of 84 compounds were identified. The most predominant components among them were linoleic acid (18.42%), l-ascorbyl 2,6-dipalmitate (11.50%), linolenic acid (10.98%) and p-cresol (9.99%) in the essential oil; and eicosane (26.34%), n-butyl acetate (21.13%), heptadecane (16.48%) and 2-pentanone, 4-hydroxy-4-methyl (11.13%) were detected in the acetone extract; heptadecane (9.40%), heneicosane (9.45%), eicosane (6.40%), n-butyl acetate (14.34%) and styrene (22.20%) were identified and quantified in the ethyl acetate extract. The cytotoxic activity of essential oil and oleoresins of micropropagated A. africana was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide) assay on Vero cells compared to the standard drug doxorubicin chloride. The present research contains primary information about the therapeutic utility of the essential oil and oleoresins of A. africana with a promising future research potential of qualitative and quantitative improvement through synchronised use of biotechnological techniques.

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