THE HEXOSAMINE AND HYDROXYPROLINE CONTENTS OF THE OVIDUCTS OF THE ESTROGENIZED IMMATURE PULLET AND OF THE NORMAL LAYING HEN

1959 ◽  
Vol 37 (1) ◽  
pp. 1081-1086 ◽  
Author(s):  
P. A. Anastassiadis

Estradiol benzoate, 1 mg per day, plus testosterone propionate, 1 mg per day, were administered to sexually immature pullets for 10 days. The dry matter, hexosamine, and hydroxyproline contents of the hypertrophied oviducts and of their major anatomical parts were in general similar to the corresponding data obtained for the oviducts of mature laying hens. Additional treatment with progesterone, 0.5 mg per day, did not affect in any definite way the weight and dry matter, hexosamine, or hydroxyproline contents of the hypertrophied oviducts of the immature pullets. Additional treatment with progesterone, 1.0 mg per day, significantly depressed the weight and dry matter contents of the hypertrophied oviducts and of their major anatomical parts, except for the vagina.

1959 ◽  
Vol 37 (9) ◽  
pp. 1081-1086 ◽  
Author(s):  
P. A. Anastassiadis

Estradiol benzoate, 1 mg per day, plus testosterone propionate, 1 mg per day, were administered to sexually immature pullets for 10 days. The dry matter, hexosamine, and hydroxyproline contents of the hypertrophied oviducts and of their major anatomical parts were in general similar to the corresponding data obtained for the oviducts of mature laying hens. Additional treatment with progesterone, 0.5 mg per day, did not affect in any definite way the weight and dry matter, hexosamine, or hydroxyproline contents of the hypertrophied oviducts of the immature pullets. Additional treatment with progesterone, 1.0 mg per day, significantly depressed the weight and dry matter contents of the hypertrophied oviducts and of their major anatomical parts, except for the vagina.


1955 ◽  
Vol 33 (1) ◽  
pp. 627-637 ◽  
Author(s):  
P. A. Anastassiadis ◽  
W. A. Maw ◽  
R. H. Common

The concentration of total hexosamine (as free base) in the dry matter of tissues of the pullet was high in cartilage (8.0 mgm. per gm.) and in oviduct (6.4 mgm. per gm.); medium in comb and wattles, tendon, and lungs (3.0 to 5.5 mgm. per gm.); and low in voluntary muscle (1.5 mgm. per gm.). Dry defatted skin contained 8.3 mgm. per gm. Total serum hexosamine was 52 mgm. per 100 ml. and was increased significantly to about 67 mgm. per 100 ml. by treatment with estradiol benzoate (ODB) and to about 72 mgm. per 100 ml. by ODB plus testosterone propionate (TST). Serum protein was also increased significantly by the hormonal treatments, but to a relatively greater extent. The magnum of the hypertrophied oviducts of pullets treated with ODB was relatively high in dry matter (22.0%), and this dry matter was relatively rich in hexosamine (13.8 mgm. per gm.) and low in hydroxyproline (2.9 mgm. per gm.). The dry matter of the uterus and vagina was relatively low in hexosamine (6.0 mgm. per gm.) and rich in hydroxyproline (5.7 and 10.5 mgm. per gm. for uterus and vagina respectively). Concurrent administration of ODB and TST led to a greater degree of hypertrophy of the oviducts than did administration of ODB alone; both the weight and total hexosamine content of the magnum showed a greater relative increase than did the values for vagina and uterus. Some modifications of the method of determining the hexosamine content of tissues are described.


1955 ◽  
Vol 33 (4) ◽  
pp. 627-637 ◽  
Author(s):  
P. A. Anastassiadis ◽  
W. A. Maw ◽  
R. H. Common

The concentration of total hexosamine (as free base) in the dry matter of tissues of the pullet was high in cartilage (8.0 mgm. per gm.) and in oviduct (6.4 mgm. per gm.); medium in comb and wattles, tendon, and lungs (3.0 to 5.5 mgm. per gm.); and low in voluntary muscle (1.5 mgm. per gm.). Dry defatted skin contained 8.3 mgm. per gm. Total serum hexosamine was 52 mgm. per 100 ml. and was increased significantly to about 67 mgm. per 100 ml. by treatment with estradiol benzoate (ODB) and to about 72 mgm. per 100 ml. by ODB plus testosterone propionate (TST). Serum protein was also increased significantly by the hormonal treatments, but to a relatively greater extent. The magnum of the hypertrophied oviducts of pullets treated with ODB was relatively high in dry matter (22.0%), and this dry matter was relatively rich in hexosamine (13.8 mgm. per gm.) and low in hydroxyproline (2.9 mgm. per gm.). The dry matter of the uterus and vagina was relatively low in hexosamine (6.0 mgm. per gm.) and rich in hydroxyproline (5.7 and 10.5 mgm. per gm. for uterus and vagina respectively). Concurrent administration of ODB and TST led to a greater degree of hypertrophy of the oviducts than did administration of ODB alone; both the weight and total hexosamine content of the magnum showed a greater relative increase than did the values for vagina and uterus. Some modifications of the method of determining the hexosamine content of tissues are described.


Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 156
Author(s):  
Ling Zhao ◽  
Yue Feng ◽  
Jing-Tao Wei ◽  
Meng-Xiang Zhu ◽  
Lei Zhang ◽  
...  

The objective of this study was to evaluate the efficacy of mycotoxin binders in reducing the adverse effects of co-occurring dietary aflatoxin B1 (AFB1), deoxynivalenol (DON) and ochratoxin A (OTA) on laying hens. Three hundred and sixty 26-week-old Roman laying hens were randomly allocated into four experimental groups with 10 replicates of nine birds each. The four groups received either a basal diet (BD; Control), a BD supplemented with 0.15 mg/kg AFB1 + 1.5 mg/kg DON + 0.12 mg/kg OTA (Toxins), a BD + Toxins with Toxo-HP binder (Toxins + HP), or a BD + Toxins with TOXO XL binder (Toxins + XL) for 12 weeks. Compared to the control, dietary supplementation of mycotoxins decreased (P < 0.10) total feed intake, total egg weight, and egg-laying rate, but increased feed/egg ratio by 2.5–6.1% and mortality during various experimental periods. These alterations induced by mycotoxins were alleviated by supplementation with both TOXO HP and XL binders (P < 0.10). Furthermore, dietary mycotoxins reduced (P < 0.05) eggshell strength by 12.3% and caused an accumulation of 249 μg/kg of DON in eggs at week 12, while dietary supplementation with TOXO HP or XL mitigated DON-induced changes on eggshell strength and prevented accumulation of DON in eggs (P < 0.05). Moreover, dietary mycotoxins increased relative liver weight, but decreased spleen and proventriculus relative weights by 11.6–22.4% (P < 0.05). Mycotoxin exposure also increased alanine aminotransferase activity and reduced immunoglobulin (Ig) A, IgM, and IgG concentrations in serum by 9.2–26.1% (P < 0.05). Additionally, mycotoxin exposure induced histopathological damage and reduced villus height, villus height/crypt depth, and crypt depth in duodenum, jejunum and (or) ileum (P < 0.05). Notably, most of these histological changes were mitigated by supplementation with both TOXO HP and XL (P < 0.05). In conclusion, the present study demonstrated that the mycotoxin binders TOXO HP and XL can help to mitigate the combined effects of AFB1, DON, and OTA on laying hen performance, egg quality, and health.


Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 701
Author(s):  
Yi Wan ◽  
Ruiyu Ma ◽  
Anam Khalid ◽  
Lilong Chai ◽  
Renrong Qi ◽  
...  

One of the most important factors that determine feed utilization by chickens is the feed form. Although it is generally believed that pellet diets have a positive effect on chicken growth, there are some studies that have indicated no difference between pellet and mash on chickens performance. This study was conducted to assess the effects of feed form on production performance, egg quality, nutrient metabolism and intestinal morphology in two breed laying hens. Two hundred and sixteen 25-week-old Hy-Line brown (n = 108) and Hy-Line grey (n = 108) hens were selected. Each breed was randomly allocated into two treatments with 6 replications (9 birds in each replication), which were fed mash and pellet diets, respectively. Production performances were recorded daily and egg quality traits were measured every two weeks. At 42 weeks of age, one bird per replication from each experimental group was selected for metabolism determination and intestine morphology observation. Compared with mash diets, pellet diets improved laying rate (p < 0.05), ADFI (average daily feed intake, p < 0.05), egg weight, shell strength, yolk proportion and Haugh unit (p < 0.05) in both breeds and reduced the FCR (feed conversion ratio, p < 0.05) in Hy-Line grey. The apparent digestibility of DM% (dry matter) and CP% (crude protein) were significantly higher (p < 0.05) in both breed laying hens fed pellet than those fed mash. The apparent digestibility of P% (phosphorus) and Ca% (calcium) was higher in Hy-Line grey fed pellet and was higher in Hy-Line brown fed mash. Compared to mash diets, pellet diets increased the VH (villus height), CD (crypt depth) and VCR (ratio of villus height to crypt depth) of the small intestine of Hy-Line grey, and increased the VH and CD of duodenum and ileum of Hy-Line brown. Overall, pellet diets improved production performance and nutrition metabolism through positive changes in the laying rate, feed intake, egg albumen quality and apparent digestibility of laying hens. The current findings provided support for the advantages of feeding pellets during the peak egg laying period for the two popular laying hen strains, Hy-Line brown and Hy-Line grey.


1960 ◽  
Vol 40 (2) ◽  
pp. 119-125 ◽  
Author(s):  
A. J. Moo-Young ◽  
K. A. McCully ◽  
R. H. Common

Inclusion of 0.5 per cent desiccated thyroid in the food of unestrogenized immature pullets for 14 days reduced thyroid weight, reduced serum Ca slightly but significantly, increased liver total crude protein, liver total DNAP and total RNAP, but did not alter the ratio RNAP:DNAP in the liver. These results are regarded as indicative of stimulation of growth of liver tissues by the thyroidal treatment.Daily intramuscular, injection of 1.0 mg. estradiol benzoate for 14 days greatly increased serum Ca and liver total crude protein, increased slightly liver total DNAP and increased greatly liver total RNAP and ratio RNAP: DNAP in the liver. The thyroidal treatment reduced estrogen-induced increase of serum Ca and of liver crude protein but did not alter significantly the effects of estrogen on liver DNAP and RNAP.The thyroidal treatment increased kidney weight in both unestrogenized and estrogenized pullets.Estrogen treatment increased kidney weight and the percentage of dry matter in the kidney.The thyroidal treatment did not affect the degree of estrogen-induced hypertrophy of the oviduct.


2010 ◽  
Vol 79 (9) ◽  
pp. S29-S34 ◽  
Author(s):  
Miroslav Macháček ◽  
Vladimír Večerek ◽  
Nora Mas ◽  
Pavel Suchý ◽  
Eva Straková ◽  
...  

The aim of this study was to assess the effects of two levels of clinoptilolite administered in feed (2% and 4%) on some selected performance indicators, metabolic utilization of basic nutrients and the health status of laying hens. The selected 24 Bovans Goldline hybrid laying hens were divided into three equal groups, two experimental groups (E1 and E2) and one control group (C). The laying hens were housed individually in cages with an automatic supply of drinking water, manual feeding, in a setting with controlled light and temperature regimens. Hens from individual groups were all fed a complete feed mix of the same composition and the only difference was in clinoptilolite supplementation: feed mixes for E1 and E2 groups contained 2% and 4% of clinoptilolite (commercial additive ZeoFeed) respectively, replacing the same amounts of wheat. The hens received feed mixes and drinking water ad libitum. During this 28-day experiment, feed consumption and the number and weight of eggs laid were monitored individually for each hen. At the end of the experiment, the balance test using the indicator method (Cr2O3) was performed in all eight hens in each of the groups. The results of balance tests were then used to calculate the metabolic utilization of selected nutrients (nitrogen, fat, ash, nitrogen-free extracts, starch, gross energy, Ca, P). After the balance tests, blood samples for haematological and biochemical examinations were collected via puncture of the vena basilica. The addition of 2% clinoptilolite to feed mix resulted in a highly significant (P ⪬ 0.01) increase in mean egg weight to 64.69 g, but the addition of 4% clinoptilolite in group E2 resulted in a highly significant (P ⪬ 0.01) decrease in mean egg weight to 62.20 g compared to the control (63.73 g). Moreover, daily feed mix consumption in group E1 decreased to 114 g per one laying hen/day compared to the controls (118 g per one laying hen/day). In group E2 (4% clinoptilolite), daily consumption of feed mix increased compared with the controls to 124 g. The 2% clinoptilolite supplementation of E1 group feed slightly increased metabolic utilization of fat, nitrogen-free extracts, starch and gross energy compared to group C. Results of haematological test of the hens’ blood showed significant changes in haemoglobin, whose values in groups C and E1 were significantly higher (P ⪬ 0.05) than in group E2. Differences in the values of the biochemical indicators monitored (total protein, glucose, cholesterol, triacylglycerols, calcium, phosphorus, magnesium and the AST enzyme) between group C and experimental groups E1 and E2 were not significant and remained within the range of reference values.


2018 ◽  
Vol 43 (3) ◽  
pp. 257 ◽  
Author(s):  
S. Kismiati ◽  
T. Yuwanta ◽  
Z. Zuprizal ◽  
S. Supadmo ◽  
U. Atmomarsono

The aim of this research was to evaluate calcium deposition in egg using eggshell flour as a limestone substitute in feed. Two hundreds laying hen of Isa Brown strain of 25 weeks were used in this study. Treatments were diet with 7.5% limestone as control (T0), 2.5% limestone is substituted with eggshell flour(T1), 5% limestone is substituted with eggshell flour (T2) and limestone is substituted with eggshell flour (T3). A completly randomized design were used to allocated the treatments with 5 replications of each. Each experimental unit consists of 10 laying hens. Parameters measured were egg weight, yolk weight, albumen weight, eggshell weight, calcium of egg (yolk, albumen and eggshell), length, weight and Ca of tibia bone. The results showed that substitution of limestone with eggshell flour had significantly effect (P<0.05) on eggshell weight, Ca deposition on yolk, albumen, and Ca of tibia bone but non significantly effect on egg weight, weight and percentage of yolk, weight and percentage of albumen, percentage and Ca of eggshell, length and weight of tibia bone. In conclusion, calcium deposition in yolk was the highest in the use of 7.5% eggshell flour to substitute limestone but obtained the lowest Ca of bone , while calcium deposition in albumen was the highest in the use of 2.5% eggshell flour.


2018 ◽  
Vol 17 (2) ◽  
pp. 63-70
Author(s):  
Ulvi Fitri Hand ◽  
Wizna . ◽  
Irfan Suliansyah ◽  
Yose Rizal ◽  
Maria Endo Mahat

1959 ◽  
Vol 39 (1) ◽  
pp. 1-5 ◽  
Author(s):  
K. A. McCully ◽  
W. A. Maw ◽  
R. H. Common

The course of sodium retention by sexually immature crossbred pullets treated with 0.5 mgm. estradiol benzoate (ODB) per day or with 0.5 mgm. ODB plus 0.4 mgm. testosterone propionate (TST) per day did not differ significantly from the course of sodium retention by untreated control pullets. The average daily retention by the pullets from 11 to 15 weeks of age was 37 mgm. Na per day.


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