Regulation of Intestinal Alkaline Phosphatase by Dietary Phosphate

1972 ◽  
Vol 50 (12) ◽  
pp. 1152-1156 ◽  
Author(s):  
L. W. McCuaig ◽  
I. Motzok
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Enzyme Activities ◽  
Inorganic Phosphate ◽  
Intestinal Alkaline Phosphatase ◽  
Dietary Phosphate ◽  
Intestinal Enzyme

Day-old chicks were fed practical rations containing varying levels of inorganic phosphate (Pi) in an attempt to study the regulation of intestinal alkaline phosphatase by phosphate. Low dietary Pi (0.16%) increased the enzyme activity by 50% compared to adequate Pi (0.48%). The enzyme activities were significantly reversed 8 days after reversing the diets. In another experiment chicks were fed 0.16 or 0.80%) Pi for 4 weeks and killed when full-fed, or after 3 days' starvation. In the full-fed birds low dietary Pi reduced the intestinal nuclear Pi and doubled the intestinal enzyme activity. Nuclear Pi was found to be inversely correlated with alkaline phosphatase in the fed animals. No such effects were found in the starved birds.

Acute Hepatopathy Associated With Mitotane Administration in a Dog

2006 ◽  
Vol 42 (4) ◽  
pp. 298-301 ◽  
Author(s):  
Craig B. Webb ◽  
David C. Twedt
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Supportive Care ◽  
Prothrombin Time ◽  
Liver Enzyme ◽  
Hepatic Failure ◽  
Enzyme Activities ◽  
Total Bilirubin ◽  
Persistent Elevation ◽  
Phosphatase Enzyme

An adult dog with a persistent elevation in alkaline phosphatase enzyme activity was started on mitotane for suspected hyperadrenocorticism. One month later, the dog was presented for intermittent anorexia and acute icterus. The dog’s liver enzyme activities and total bilirubin were markedly elevated, prothrombin time was prolonged, and blood urea nitrogen and glucose were low. Histopathology revealed marked, centrilobular, hepatocellular loss. After discontinuation of the mitotane, the dog recovered with supportive care and was normal 3 months later, which was consistent with mitotane-associated hepatic failure.

The Calf Intestinal Alkaline Phosphatase. II. Reaction between the metal content and the enzyme activity

1983 ◽  
Vol 66 (3) ◽  
pp. 871-879 ◽  
Author(s):  
Plato Portmann ◽  
Hubert Schaller ◽  
Geneviève Leva ◽  
Werner Venetz ◽  
Thomas Müller
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Metal Content ◽  
Intestinal Alkaline Phosphatase ◽  
Calf Intestinal Alkaline Phosphatase

ELECTRON MICROSCOPY OF ALKALINE PHOSPHATASE OF ESCHERICHIA COLI

1966 ◽  
Vol 12 (4) ◽  
pp. 605-607 ◽  
Author(s):  
V. M. Kushnarev ◽  
T. A. Smirnova
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Cell Wall ◽  
Enzyme Activity ◽  
Electron Microscope ◽  
Inorganic Phosphate ◽  
E Coli

A method is described for determining the localization of alkaline phosphatase in the cells of E. coli B with the electron microscope. Enzyme activity, determined by deposition of inorganic phosphate, is located in the exterior layer of the cell wall.

Interactions of Ca, P, Zn, and Alkaline Phosphatase in the Chick. II. Effect of Dietary Ca Level

1974 ◽  
Vol 52 (1) ◽  
pp. 90-95 ◽  
Author(s):  
L. W. McCuaig ◽  
I. Motzok
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Inorganic Phosphate ◽  
Duodenal Mucosa ◽  
Positive Correlation ◽  
Mn Content

Chicks were fed a semi-purified diet, containing 0.75% Ca, 0.5% inorganic phosphate (Pi), and 50 p.p.m. Zn, to 2 weeks of age. They were then divided into groups and fed the same diet with 0, 0.75, 1.50, 2.25, or 3.00% Ca, for 4 or 7 days before being killed. Plasma was analyzed for Ca, Pi, and Zn, and duodenal mucosa for Ca, Pi, Zn, Mn, and alkaline phosphatase (AP). The low Ca treatment decreased plasma and mucosa Ca and increased mucosa Zn, whereas the high Ca diets increased mucosa Ca and decreased mucosa Zn, plasma Pi, and mucosa AP activity. There was an overall positive correlation of AP with plasma and mucosa Zn, and plasma and mucosa Pi, and a negative overall correlation of AP with plasma and mucosa Ca. Mucosa Mn content did not seem to be related to the enzyme activity.

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: III. THE EFFECTS OF VARIOUS FATTY ACIDS ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1955 ◽  
Vol 33 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Nester Dickie ◽  
Margaret I. Robinson ◽  
Jules Tuba
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Oleic Acid ◽  
Saturated Fatty Acids ◽  
Wheat Gluten ◽  
Dietary Fatty Acids ◽  
Intestinal Alkaline Phosphatase ◽  
Intestinal Enzyme ◽  
Fasted Rats

Fasted rats were used in a study of the effect of fatty acids on intestinal and serum phosphatase. Each acid was mixed with a palatable carrier, and then offered to the animals. Enzyme estimations were made with intestinal homogenates, and occasionally with serum. The first carrier, casein, was found to affect intestinal phosphatase activity, and it was replaced by a non-active carrier, wheat gluten. The results with the two carriers showed essentially the same trend. The elevation of intestinal alkaline phosphatase by dietary fatty acids varied inversely with the chain length for: butyric acid, lauric acid, palmitic acid, and stearic acid. The possibility is considered that decreasing solubility may be associated with the response obtained with these fatty acids. Oleic acid was a more powerful stimulant for the synthesis of intestinal alkaline phosphatase than the saturated fatty acids. Choline, fed simultaneously with oleic acid, completely obliterated the highly significant effect of the acid on both the intestinal and the serum enzyme, These findings indicate that further consideration should be given to the role of phospholipids in absorption of fatty acids. Enzyme response in the serum, studied with a more limited number of fatty acids, lagged a few hours behind the response of the intestinal enzyme.

scholarly journals A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis

2018 ◽  
Vol 43 (5) ◽  
pp. 1409-1424 ◽  
Author(s):  
Shohei Sasaki ◽  
Hiroko Segawa ◽  
Ai Hanazaki ◽  
Ruri Kirino ◽  
Toru Fujii ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Inorganic Phosphate ◽  
Intestinal Alkaline Phosphatase ◽  
Phosphate Homeostasis

Effect of fat feeding on intestinal alkaline phosphatase activity in tissue and serum

1981 ◽  
Vol 241 (6) ◽  
pp. G461-G468 ◽  
Author(s):  
G. P. Young ◽  
S. Friedman ◽  
S. T. Yedlin ◽  
D. H. Allers
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Small Bowel ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Intestinal Alkaline Phosphatase ◽  
Serum Enzyme ◽  
Small Bowel Mucosa ◽  
Fat Feeding ◽  
Bowel Mucosa

Serum intestinal alkaline phosphatase activity is increased by fat feeding, but the mechanism of this increase is not fully understood. Fasting rats were fed a single feed of either corn oil (12 kcal) or an isocaloric elemental feed (Vivonex 100 HN). Changes in enzyme activity in the small bowel mucosa and serum were followed for 20 h. Only the fat-fed rats had increased serum enzyme activity, being maximal at 7 h and three times the fasting level. This resulted from an increase in the amount of enzyme protein in the serum and not from an increase in its catalytic efficiency. The serum biological half-life of 125I-labeled intestinal alkaline phosphatase was the same in fasted (2.51 min) and fat-fed rats (2.55 min). Both types of feed caused a quantitatively similar increase in brush-border-bound alkaline phosphatase activity. However, levels of soluble intracellular alkaline phosphatase in intestinal mucosa were affected differently: the elemental diet caused a substantial rise, whereas no significant change was seen after fat feeding. The isoelectric pattern of phosphatase activity in serum after fat feeding was identical to that of soluble intracellular and not membranous alkaline phosphatase. Therefore, serum intestinal alkaline phosphatase activity rises in response to a single fat feed as a result of increased delivery of the enzyme to the blood and not as a result of an increase in its normally short biological half-life. This rise cannot be directly linked to an increase in the amount of brush-border-bound enzyme, and it appears that the serum enzyme is derived directly from a pool of soluble intracellular enzyme in the small bowel mucosa.

Intestinal enzyme adaptation to normal diets of different composition

1980 ◽  
Vol 239 (6) ◽  
pp. G445-G451 ◽  
Author(s):  
D. M. McCarthy ◽  
J. A. Nicholson ◽  
Y. S. Kim
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activities ◽  
High Fat Diet ◽  
Ileal Mucosa ◽  
Peptide Hydrolase ◽  
Enzyme Adaptation ◽  
High Starch ◽  
Intestinal Enzyme ◽  
High Protein Diets ◽  
Protein Diets

Groups of rats were fed isocaloric amounts of diets rich in starch, protein, or fat. After 7 days, activities of sucrase, maltase, lactase, alkaline phosphatase, L-phenylalanylglycine hydrolase, and L-leucyl-L-alanyl hydrolase were measured in the duodenal, jejunal, and ileal mucosa of fed rats. Peptide hydrolase and alkaline phosphatase activities were significantly higher in rats fed high-protein diets, particularly in the ileum. Disaccharidases were highest in rats fed high-starch diets, especially in jejunum; the lowest activities of peptide hydrolase were seen with this diet. The high-fat diet was generally associated with intermediate activities of most enzymes, except disaccharidases, which were lowest on this diet. The different macromolecular nutrients were associated with adaptive differences in intestinal enzyme activities, which were biologically appropriate and coordinated with similar known changes in pancreatic and other enzyme activities concerned with assimilation of the particular food. However, other less interpretable changes occurred, which may have been due to effects on pancreatic secretion or on the metabolism, growth, and differentiation of intestinal cells.

Automated Determination of Serum Alkaline Phosphatase Using a Modified Bodansky Technic

1964 ◽  
Vol 10 (1) ◽  
pp. 75-82 ◽  
Author(s):  
H Keay ◽  
J A Trew
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Inorganic Phosphate ◽  
Serum Alkaline Phosphatase ◽  
Automated Determination

Abstract An automated procedure for the measurement of alkaline phosphatase activity by a modification of the Bodansky method is described. It has been possible to adapt the Fiske-SubbaRow method for phosphate so that alkaline phosphatase is determined by a second run, immediately following the determination of inorganic phosphate. Studies of the effect of time, pH, and concentration of barbital on the enzyme activity are discussed, and the advantages of the method are listed.

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