Effect of changes in the red cell volume on the carbon dioxide titration curve in vivo in the rat

1981 ◽  
Vol 59 (5) ◽  
pp. 500-503
Author(s):  
S. Kaufman ◽  
C. T. Kappagoda

Acute in vivo CO2 titration curves were performed on rats anaesthetized with pentobarbitone. The slope of the in vivo CO2 titration curve in the rat was found to be similar to that previously reported in the dog and in man. Removal of approximately 30% of the haemoglobin of the body did not influence significantly the slope of the in vivo CO2 titration curve in the rat.

1968 ◽  
Vol 19 (1) ◽  
pp. 145 ◽  
Author(s):  
PH Springell

Twenty-four steers, comprising British (Herefords and Hereford x Shorthorn crosses), Zebu (Africander), and Zebu cross (British x Brahman or Africander) breeds, were maintained either on pasture or yarded, and fed on diets of a low and a high nutritional value. Blood volumes were determined on five occasions at intervals of 3 months by the 51Cr labelling technique, plasma and red cell volumes being then derived from the venous haematocrit. The blood plasma, and red cell volumes are all very significantly, correlated with, and represent respectively 4.97, 3.27, and 1.70% of, the fasting body weight. To avoid the confounding effect of body weight, the parameters are expressed as "contents", i.e, in terms of volume per kilogram fasting body weight. In the grazing group breed differences were generally absent. This may in part be due to the fact that the Zebu crossbreds belonged mostly to the F2 and partly to the F3 generation. In the yarded group, where F1 crossbreds were compared with British steers, breed differences were more frequent. British steers tended to have higher plasma contents, but lower red cell contents and haematocrits. Nutrition had no effect on plasma contents, but good nutrition was generally associated with higher haematocrits, as well as with elevated blood and red cell contents. Seasonal differences were in evidence, and all parameters generally reached minimal values in winter or spring. The significance of these findings in relation to adaptation to a tropical environment is discussed. The haematocrit does not necessarily reflect changes in the red cell volume. There is also some indication that the water and plasma contents may be related. The possible usefulness of the red cell volume for predicting the body composition is discussed.


1965 ◽  
Vol 16 (4) ◽  
pp. 661 ◽  
Author(s):  
BA Panaretto ◽  
DA Little

The relationship between total body water and red cell volume was determined in a group of non-pregnant crossbred ewes, in an endeavour to obtain a method which did not necessitate the use of radioisotopes for determining body composition. Total body water could be predicted from red cell volume. The 95% confidence interval for a mean value for total body water, predicted from the red cell volume, was ±18.8% of the mean. All other relations which can be derived between the red cell volume and other body parameters depended on this relation. The calculation of body composition from measurements of the red cell volume is discussed.


1954 ◽  
Vol 123 (2) ◽  
pp. 367-376 ◽  
Author(s):  
D. Mary Jackson ◽  
Marjorie E. Nutt

1957 ◽  
Vol 191 (1) ◽  
pp. 159-162 ◽  
Author(s):  
S. Deavers ◽  
R. A. Huggins ◽  
E. L. Smith

Dogs anesthetized with morphine and sodium pentobarbital were killed by bleeding or with sodium pentobarbital. The control group did not receive a transfusion; the other group was given a transfusion of blood (10.0–14.9% of the body weight). The red cell volume was measured with radioactive Cr51 and the plasma volume with I131. The volume of red cells and plasma per gram of tissue and organ were calculated for: liver, spleen, kidney, heart, lung, stomach, intestine, muscle and skin. With transfusion there was an increase in the red cell volume per gram of tissue and organ except for the spleen. The liver was the primary site of trapped cells. All tissues of the transfused dogs contained more plasma than the controls. Every organ of the control dogs contained ‘extra’ plasma (that portion of the plasma free from cells); with transfusion the volume increased and represented a greater portion of the total organ blood volume. Good agreement was found between the calculation of trapped cells and plasma from blood volume measurements and by tissue analysis.


1957 ◽  
Vol 190 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Joseph F. Garcia

Using the Fe59-labeled cell dilution technique, the blood, plasma and red cell volume and total circulating hemoglobin have been determined on 259 male rats varying in age from 1 to 340 days. An anemic period exists in the male rat which is maximal between 15 and 20 days of age. This anemia is observed in terms of a decrease in the volume of red blood cells per gram of rat as well as in hematocrit and hemoglobin concentration values. However, throughout this anemic period there is a continual increase in total red cell volume and, in fact, the daily gain of red blood cells per gram of rat is greater during this period than at any time thereafter. In spite of the very high rate of erythropoiesis occurring at this time the growth of the body mass as a whole exceeds it and so for a time anemia results.


Blood ◽  
1961 ◽  
Vol 17 (1) ◽  
pp. 71-82 ◽  
Author(s):  
HUGH FUDENBERG ◽  
MARIO BALDINI ◽  
JOHN P. MAHONEY ◽  
WILLIAM DAMESHEK

Abstract 1. Red cell volume and plasma volume were measured by the radioactive sodium chromate and the chromic chloride method respectively in 37 patients. 2. The body hematocrit/venous hematocrit (BH/VH) ratio was found to be constant in normal subjects and in patients in whom there was neither splenomegaly nor edema. The ratio was 0.896 ± 0.039. 3. In splenectomized patients the BH/VH ratio was normal indicating that in man, in normal conditions, the spleen does not have the function of a significant blood reservoir with raised cell/plasma ratio. 4. In patients with splenomegaly the BH/VH ratio was found to be significantly increased in direct proportion to the degree of splenic enlargement, indicating an increased concentration of erythrocytes in the enlarged spleen. 5. In the presence of extravascular fluid retention caused by prolonged prednisone treatment the BH/VH ratio was lower than normal. 6. It was concluded that in patients with splenomegaly or edema measurement of either red cell volume or plasma volume alone in conjunction with the "corrected" venous hematocrit does not permit valid assessment of the non-measured parameter nor of the total blood volume.


1975 ◽  
Vol 48 (2) ◽  
pp. 133-138
Author(s):  
J. B. Stoker ◽  
C. T. Kappagoda ◽  
H. M. Snow ◽  
R. J. Linden

1. Carbon dioxide titration curves were determined in vivo in dog and man at various degrees of acute non-respiratory acidaemia and alkalaemia. 2. The slope of the CO2 titration curve (Δlog Pco2/ΔpH) was found to increase with the severity of the acute non-respiratory acidaemia. In states of acute non-respiratory alkalaemia the slope (Δlog Pco2/ΔpH) tended towards unity. 3. A simple scheme based on the CO2 titration curves determined in vivo has been proposed for the assessment of acute acid-base disturbances in man. 4. Carbon dioxide titration curves were also determined in vivo in patients with chronic respiratory and non-respiratory acidaemia and it was found that these curves were not significantly different from those obtained in states of acute acid-base disturbances. It is therefore suggested that the scheme described in this paper is applicable to all acid-base disturbances.


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