Changes in Cell and Plasma Volumes of Various Organs Produced by a Massive Transfusion in Dogs

1957 ◽  
Vol 191 (1) ◽  
pp. 159-162 ◽  
Author(s):  
S. Deavers ◽  
R. A. Huggins ◽  
E. L. Smith
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
The Body ◽  
Control Group ◽  
Sodium Pentobarbital ◽  
Tissue Analysis ◽  
Red Cell ◽  
Red Cell Volume ◽  
Volume Measurements ◽  
Good Agreement

Dogs anesthetized with morphine and sodium pentobarbital were killed by bleeding or with sodium pentobarbital. The control group did not receive a transfusion; the other group was given a transfusion of blood (10.0–14.9% of the body weight). The red cell volume was measured with radioactive Cr51 and the plasma volume with I131. The volume of red cells and plasma per gram of tissue and organ were calculated for: liver, spleen, kidney, heart, lung, stomach, intestine, muscle and skin. With transfusion there was an increase in the red cell volume per gram of tissue and organ except for the spleen. The liver was the primary site of trapped cells. All tissues of the transfused dogs contained more plasma than the controls. Every organ of the control dogs contained ‘extra’ plasma (that portion of the plasma free from cells); with transfusion the volume increased and represented a greater portion of the total organ blood volume. Good agreement was found between the calculation of trapped cells and plasma from blood volume measurements and by tissue analysis.

PLACENTAL TRANSFUSION AND HYPERBILIRUBINEMIA IN THE PREMATURE

PEDIATRICS ◽  
1972 ◽  
Vol 49 (3) ◽  
pp. 406-419 ◽  
Author(s):  
Saroj Saigal ◽  
Allison O'Neill ◽  
Yeldandi Surainder ◽  
Le-Beng Chua ◽  
Robert Usher
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Premature Infants ◽  
Full Term ◽  
Neonatal Hyperbilirubinemia ◽  
Red Cell ◽  
Term Infants ◽  
Red Cell Volume ◽  
Cord Clamping ◽  
Volume Measurements

Placental transfusion has been compared in premature and full-term infants. Blood volume measurements showed that the 5-minute transfusion was similar in full-term and premature infants (47% and 50% increase in blood volume from birth). A larger proportion of the 5-minute transfusion occurred by 1 minute in full-term (76%) than in premature infants (56%). Placental transfusion, by increasing red cell volume, greatly enhanced the severity of neonatal hyperbilirubinemia. Bilirubin concentrations of 15 mg/100 ml developed in only 6% of premature infants when cord clamping was immediate, in 14% when cord clamping was delayed 1 minute, and in 38% after a 5-minute delay in cord clamping.

Splenic red cell sequestration and blood volume measurements in conscious pigs

1985 ◽  
Vol 248 (3) ◽  
pp. R293-R301 ◽  
Author(s):  
J. P. Hannon ◽  
C. A. Bossone ◽  
W. G. Rodkey
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Physical Restraint ◽  
Red Cell ◽  
Red Cell Volume ◽  
Epinephrine Injection ◽  
Volume Measurements

When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.

scholarly journals Blood volume and hemoglobin mass in long-term heart transplant recipients with and without Anemia

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Florian Seiler ◽  
Christoph Ahlgrim ◽  
Philipp Birkner ◽  
Nina Wrobel ◽  
Jonathan Rilinger ◽  
...  
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Heart Transplant ◽  
Control Group ◽  
Red Cell ◽  
Transplant Recipients ◽  
Red Cell Volume ◽  
Heart Transplant Recipients

Abstract Background In systolic chronic heart failure, a heterogeneous blood volume (BV) regulation can be found with plasma volume expansion in many cases, possibly leading to pseudoanemia. Little is known about the volume status after heart transplantation (HTX). So far, anemia of HTX recipients was solely investigated using hemoglobin-concentration that may be misleading in a clinical context. The objective of the study was whether a difference in plasma volume and red cell volume can be observed in clinically stable heart transplant recipients compared to matched control subjects. Secondary, the aim was to describe anemia in the long-term after HTX based on quantitative data. Methods Blood volume and its constituents red cell volume and plasma volume were quantified using an abbreviated carbon monoxide rebreathing method (aCORM) with focus on its primary measure total hemoglobin mass (Hbmass) and coincidental anemia in 36 (7 women) heart transplant recipients. For comparison, a matched control group of 46 (5 women) healthy subjects was selected. Results Neither Hbmass nor blood volumes were significantly different in HTX patients compared to matched healthy control group subjects. The prevalence of anemia 6.3 ± 4.3 years after transplantation was 19%. Hbmass and red cell volume were significantly lower in anemic HTX patients compared to non-anemic patients while plasma volume was not expanded. Various immunosuppressant regimens did not have an effect on Hbmass, plasma volume or red cell volume. Conclusions There was no difference in blood volumes and Hbmass between HTX patients and control subjects. The pathophysiologic blood volume regulation in chronic heart failure does not seem to be longer active in long-term HTX recipients. However, in the long-term after HTX, anemia occurs in a considerable number of patients as true anemia without a clear association with immunosuppression. Trial registration German registry for clinical studies, DRKS00006078. Registered 09 May 2014, https://www.drks.de/drks_web/navigate.do?navigationId=trial. HTML&TRIAL_ID=DRKS00006078.

Blood Volume Measurement in Baboons: Simultaneous Estimation of Red Cell Volume and Plasma Volume

1985 ◽  
Vol 14 (6) ◽  
pp. 345-356
Author(s):  
Michael G. Garner ◽  
Andrew F. Phippard ◽  
John S. Horvath ◽  
Geoffrey G. Duggin ◽  
David J. Tiller
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Volume Measurement ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Volume Measurement

Red cell volume and blood volume in beef cattle

1968 ◽  
Vol 19 (1) ◽  
pp. 145 ◽  
Author(s):  
PH Springell
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Nutritional Value ◽  
The Body ◽  
Red Cell ◽  
Confounding Effect ◽  
Red Cell Volume ◽  
Breed Differences ◽  
Good Nutrition ◽  
Cell Volumes

Twenty-four steers, comprising British (Herefords and Hereford x Shorthorn crosses), Zebu (Africander), and Zebu cross (British x Brahman or Africander) breeds, were maintained either on pasture or yarded, and fed on diets of a low and a high nutritional value. Blood volumes were determined on five occasions at intervals of 3 months by the 51Cr labelling technique, plasma and red cell volumes being then derived from the venous haematocrit. The blood plasma, and red cell volumes are all very significantly, correlated with, and represent respectively 4.97, 3.27, and 1.70% of, the fasting body weight. To avoid the confounding effect of body weight, the parameters are expressed as "contents", i.e, in terms of volume per kilogram fasting body weight. In the grazing group breed differences were generally absent. This may in part be due to the fact that the Zebu crossbreds belonged mostly to the F2 and partly to the F3 generation. In the yarded group, where F1 crossbreds were compared with British steers, breed differences were more frequent. British steers tended to have higher plasma contents, but lower red cell contents and haematocrits. Nutrition had no effect on plasma contents, but good nutrition was generally associated with higher haematocrits, as well as with elevated blood and red cell contents. Seasonal differences were in evidence, and all parameters generally reached minimal values in winter or spring. The significance of these findings in relation to adaptation to a tropical environment is discussed. The haematocrit does not necessarily reflect changes in the red cell volume. There is also some indication that the water and plasma contents may be related. The possible usefulness of the red cell volume for predicting the body composition is discussed.

Circulating and tissue hematocrits of normal unanesthetized mice

1959 ◽  
Vol 196 (2) ◽  
pp. 420-422 ◽  
Author(s):  
Julius J. Friedman
Keyword(s):  
Serum Albumin ◽  
Cell Volume ◽  
Plasma Volume ◽  
Venous Blood ◽  
Red Cells ◽  
Red Cell ◽  
Volume Data ◽  
Red Cell Volume ◽  
Volume Measurements ◽  
Total Body

The circulating and tissue hematocrits of normal unanesthetized mice were determined by means of independent red cell and plasma volume measurements. The red cell volume-indicator which was used in this study was radioiron (Fe59) tagged red cells. The plasma volume data were derived by means of radioiodine (I131) labeled serum albumin and were reported earlier (Friedman, Proc. Soc. Exper. Biol. & Med. 88: 323, 1955). The hematocrits of the various tissues were found to be: for spleen 51.3, lung 47.9, muscle 49.9, liver 38.9, intestine, 32.2, skin 29.2 and kidney 24.0%. The total body hematocrit was 35.4% as compared to 48.4 for venous blood. All tissues, with the exception of spleen and lung, contained hematocrits which were lower than that of venous blood suggesting the presence of some mechanism within the various tissues which is capable of effectively separating plasma from red cells.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

Abstract The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

A COMPARATIVE STUDY OF RED CELL DIAMETER AND RED CELL VOLUME MEASUREMENTS

The Lancet ◽  
1934 ◽  
Vol 223 (5767) ◽  
pp. 513-517 ◽  
Author(s):  
JanetM. Vaughan ◽  
HelenM. Goddard
Keyword(s):  
Comparative Study ◽  
Cell Volume ◽  
Cell Diameter ◽  
Red Cell ◽  
Red Cell Volume ◽  
Volume Measurements

Changes of serum and blood volumes during gestation and lactation in multiparous sows

1996 ◽  
Vol 76 (2) ◽  
pp. 263-266 ◽  
Author(s):  
J. J. Matte ◽  
C. L. Girard
Keyword(s):  
Key Words ◽  
Cell Volume ◽  
Blood Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Lactating Sows ◽  
Volume Blood ◽  
Blood Volumes

Changes of serum and plasma volumes were determined in 36 gestating sows and 20 lactating sows at their second parity. There was no change (P > 0.05) in blood and serum volumes between 4 wk pre-mating and 1 wk post-mating. During gestation, blood and serum volumes increased by approximately 25%, with most of this increase occurring between 11 and 14 wk of pregnancy (P < 0.006). From parturition to weaning at 4 wk of lactation, serum and blood volumes decreased linearly (P < 0.02) by approximately 9%. Key words: Serum volume, blood volume, packed red cell volume, sow

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