The effect of neonatal treatment of rats with nerve growth factor on the blood pressure and structure of the mesenteric arteries

1992 ◽  
Vol 70 (8) ◽  
pp. 1154-1160 ◽  
Author(s):  
Robert M. K. W. Lee ◽  
Michael Coughlin ◽  
Jim Tsoporis ◽  
Chiu-Yin Kwan ◽  
Yong-Yuan Guan ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Wistar Rats ◽  
Mesenteric Arteries ◽  
Nerve Growth ◽  
Neonatal Treatment ◽  
Male Wistar Rats ◽  
And Control

Newborn male Wistar rats were treated with nerve growth factor daily by subcutaneous injection for 2 weeks, and control rats were treated with either cytochrome c or buffered saline. Average body weight of the treated animals was lower than that of the controls during the 2 weeks of treatment, but became similar to that of the controls thereafter. Tissue levels of norepinephrine were elevated in the brain, adrenal glands, mesenteric arteries, and vas deferens of the treated animals immediately after the treatment, but became similar in the three groups 2 weeks after the termination of the treatment. Blood pressure and heart rate were measured beginning at 4 weeks of age until 28 weeks, when the rats were sacrificed and the mesenteric arteries sampled for morphometric measurements of vessel wall dimensions. Pretreatment with nerve growth factor did not affect blood pressure, nor heart rate. Structural alteration of the three types of mesenteric arteries was also absent in the treated animals. We conclude that even though neonatal treatment of normal Wistar rats with nerve growth factor for 2 weeks induced an elevation of the norepinephrine levels in several tissues at the end of the treatment period, it was not sufficient to produce hypertension and structural alterations in the blood vessels.Key words: nerve growth factor, sympathetic nerve, hypertension, vascular changes, morphometry.

scholarly journals Hemodynamic and thermoregulatory effects of xylazine-ketamine mixture persist even after the anesthetic stage in rats

2012 ◽  
Vol 64 (4) ◽  
pp. 860-864 ◽  
Author(s):  
C. Picollo ◽  
A.J. Serra ◽  
R.F. Levy ◽  
E.L. Antonio ◽  
L. dos Santos ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Diastolic Blood Pressure ◽  
Rectal Temperature ◽  
Wistar Rats ◽  
Complete Recovery ◽  
Anesthetic Depth ◽  
Awake State ◽  
Male Wistar Rats ◽  
And Control

The xylazine-ketamine mixture (KX) is an anesthetic approach commonly administered to assess cardiovascular function in rodents. This study aimed to examine if the cardiovascular and thermoregulatory effects of KX could persist after the anesthetic state ceased in rats. Male Wistar rats were anesthetized with K (50mg/kg) X (10mg/kg) through the intra-peritoneal route. Hemodynamic and thermoregulatory repercussions were evaluated in animals in awake state, during an anesthetic depth and after complete recovery of anesthetized state. KX was efficient to significantly induce deep anesthesia in all rats after 10min. A complete recovery of anesthetized state was observed only after 210min. Compared with preanesthetic state and control animals that received no drug, KX induced a significant reduction of systolic and diastolic blood pressure at 10min. Hypotension was more prominent at 150min. The heart rate was also significantly reduced after 10 min of KX and the highest magnitude of bradycardia was observed at 30min. In addition, rectal temperature was markedly decreased at 30min of KX and the higher reduction occurred at 150min. The hemodynamic and thermoregulatory effects of KX were maintained even after complete anesthetic recovery.

The effect of nerve growth factor on nuclear progression of porcine oocytes during in vitro maturation and embryo development

2005 ◽  
Vol 53 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Ágnes Bali Papp ◽  
T. Somfai ◽  
Mabel Tartaglione ◽  
Erika Varga ◽  
J. C. Gardon
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
In Vitro Maturation ◽  
Developmental Competence ◽  
Control Groups ◽  
Cell Stage ◽  
Nerve Growth ◽  
Morula Stage ◽  
And Control

The present study examined the effect of nerve growth factor (NGF) on in vitro maturation (IVM), in vitro fertilisation (IVF) and subsequent embryonic development of porcine oocytes. Cumulus-oocyte complexes were cultured with or without 1.0 ng/ml NGF for 40 h. After IVF, they were cultured in vitro for 6 days. After 10 and 20 h of IVM, there was no difference in nuclear status between the NGF-treated and control oocytes. Significant differences were detected in nuclear progression of oocytes matured in the presence or absence of NGF at 30 h of culture. A higher proportion of NGF-treated oocytes were at M-II stage compared to the control. Nevertheless, at the end of the 40-h IVM period, there was no difference in the proportion of M-II stage oocytes between the NGF-treated and control groups. NGF in IVM medium did not influence the developmental competence of putative embryos. Most embryos remained at the 2- to 4-cell stage; however, a significant amount of embryos reached the morula stage both in the NGF and the control groups. These results suggest that NGF during IVM accelerates nuclear progression of porcine oocytes by enhancing the post-diakinetic events of meiosis.

scholarly journals To compare the blood pressure and heart rate during course of various types of anesthesia in wistar rat: A novel experiences

2018 ◽  
Vol 9 (6) ◽  
pp. 37-39
Author(s):  
Jagdish Narayan ◽  
Pradeep Kumar ◽  
Ankit Gupta ◽  
Sunita Tiwari
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Body Weight ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Medical Sciences ◽  
The Awakening ◽  
Group I ◽  
Male Wistar Rats ◽  
Thiopental Sodium

Background: Rats are commonly used animals in development of newer drugs, rectification of toxicity and to record the various alterations in physiological parameters following pharmacological and non pharmacological interventions.Aim and Objectives: The purpose of this study was to identify the best physiological window during anesthesia. Therefore, we compared the effect of anesthesia using combination of ketamine and xylazine (KX) and thiopental sodium (intraperitoneally) on blood pressure and heart rate in adult male Wistar rats. Material and Methods: Twelve, male Wistar rats with a mean body weight of 260 ± 15 g were acquired. Thiopental sodium and cocktail of ketamine and xylazine (KX) were administered (ip) in group- I and group-II respectively. The systolic blood pressure and heart rate was recorded in both the groups till the awakening phase.Results: We found that there was a constant SBP and HR in Ketamine/Xylezine groups that are from 30 to 90 minutes after injection of anesthesia while this window was not observed in thiopental group.Conclusion: Our study concludes that the best time to observe the effect of newer drug during period between 30- 90 minutes after anesthesia.Asian Journal of Medical Sciences Vol.9(6) 2018 37-39

scholarly journals Poor response of cultured mast cells derived from mi/mi mutant mice to nerve growth factor

Blood ◽  
1994 ◽  
Vol 84 (9) ◽  
pp. 2977-2983 ◽  
Author(s):  
T Jippo ◽  
H Ushio ◽  
S Hirota ◽  
H Mizuno ◽  
A Yamatodani ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mast Cells ◽  
Leucine Zipper ◽  
Poor Response ◽  
Mutant Mice ◽  
Nerve Growth ◽  
Helix Loop Helix ◽  
Suboptimal Dose ◽  
And Control

Decreased numbers of mast cells and abnormalities in the phenotype of mast cells are observed in the skin of mi/mi mutant mice. Recently, the mi locus was identified to encode a novel member of the basic-helix- loop-helix-leucine zipper protein family of transcription factors. Since nerve growth factor (NGF) has been reported to influence the proliferation and the phenotype of cultured mast cells (CMCs), we compared the effect of NGF between mi/mi and control normal (+/+) CMCs. Addition of NGF to the suboptimal dose of recombinant murine interleukin-3 (rmIL-3) increased the plating efficiency of +/+ CMCs, but not of mi/mi CMCs. Although +/+ CMCs were berberine sulfate- negative when cultured with rmIL-3 alone, +/+ CMCs became berberine sulfate-positive when cultured in the presence of both rmIL-3 and NGF, which suggests increased heparin content. In contrast, NGF did not influence the phenotype of mi/mi CMCs. +/+ CMCs significantly bound 125I-NGF, but mi/mi CMCs did not, which suggests a defect of NGF receptors in mi/mi CMCs. Both p75 and p140 molecules are known to be involved in the formation of NGF receptors. Although the expression of p140 messenger (m)RNA was comparable between +/+ and mi/mi CMCs, the expression of p75 mRNA was significantly lower in mi/mi CMCs than in +/+ CMCs. Taken together, the poor response of mi/mi CMCs to NGF appeared to be attributable to the impaired transcription of the p75 gene.

scholarly journals Localisation and hypersecretion of Nerve Growth Factor in breast Phyllodes tumours: evidence from a preliminary study

2018 ◽  
Author(s):  
Ashutosh Kumar ◽  
Khursheed Raza ◽  
Tapas C. Nag ◽  
Anurag Srivastava ◽  
Ritu Sehgal
Keyword(s):  
Breast Cancer ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Histopathological Diagnosis ◽  
Nerve Growth ◽  
Phyllodes Tumours ◽  
And Control

AbstractBackgroundThe pathophysiology of the breast phyllodes tumours is uncertain. Currently, wide surgical removal is the only available treatment option. The histopathological diagnosis of phyllodes tumours is often confused with that of fibroadenomas due to a striking histological resemblance; hence a distinctive biomarker for this tumour type is warranted.Material & MethodsFresh human breast tissue was obtained from surgically excised breast phyllodes and fibroadenoma tumours (test, 2 cases each), breast cancer (positive control, 2 cases) and normal breast tissue (negative control, 1 case). Immunohistochemistry was performed for the detection of nerve growth factor (NGF) on frozen sections of the test and control tissues fixed in 4% paraformaldehyde, using the indirect streptavidin-biotin-peroxidase complex method. Sandwich ELISA on tissue homogenates of the same test and control cases was also performed to validate the immunohistochemical findings.ResultsA marked difference in NGF expression was detected in phyllodes tumours compared to fibroadenomas. The maximum NGF expression was observed in phyllodes tissue followed by cancer tissue, and the least expression in fibroadenomas (3-5 times less than in phyllodes; comparable with normal breast tissue).ConclusionNGF is known for its growth inducing potential in breast cancer, but its secretion by a benign breast tumour is not known in literature. This study reports abundant NGF secretion by breast phyllodes, raising the possibility of its potential role in tumour pathogenesis and progression that can be exploited therapeutically in future. We also propose that NGF may be used as a distinct biomarker of phyllodes tumours, for differentiating them from fibroadenomas during histopathology.

scholarly journals Nerve growth factor protects cholinergic neurons against quinolinic acid-induced excitotoxicity in wistar rats

2004 ◽  
Vol 54 (2-3) ◽  
pp. 105-113
Author(s):  
Vasiljevic Ivana ◽  
Jovanovic Marina ◽  
Colic Miodrag ◽  
Micic D. ◽  
Ninkovic Milica ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Quinolinic Acid ◽  
Wistar Rats ◽  
Cholinergic Neurons ◽  
Nerve Growth
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