The effect of metabolic rate on stable carbon and nitrogen isotope compositions in deer mice, Peromyscus maniculatus
The stable isotopic compositions of nitrogen and carbon in animal tissues reflect diet. However, factors other than diet can also affect these stable isotope ratios, leading to misinterpretations of diet composition. To test the hypothesis that variation in metabolic rate alters the isotopic compositions of tissues, deer mice ( Peromyscus maniculatus (Wagner, 1845)) were kept at three temperatures (thermoneutral (23 °C), cool (5 °C), and cold (–10 °C)) and fed ad libitum. The changes in carbon and nitrogen isotope compositions of liver associated with the thermoneutral versus cool and cold conditions were very small in comparison with those arising from differences in diet. We conclude that temperature-induced variations in metabolic rate are insufficient to produce differences in the stable carbon or nitrogen isotope compositions that could be mistaken for changes in diet.