COMPUTER ANALYSIS AND RECOGNITION OF FUNCTIONAL SITES VIA OLIGONUCLEOTIDE PATTERN DISTRIBUTIONS

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

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Computer analysis of side-band holography in STEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100087732 ◽

1991 ◽

Vol 49 ◽

pp. 684-685

Author(s):

M.A. Gribelyuk ◽

J.M. Cowley

Keyword(s):

Interference Pattern ◽

Computer Analysis ◽

Side Band ◽

Diffraction Plane ◽

Illumination System ◽

Probe Size

Recently the use of a biprism in a STEM instrument has been suggested for recording of a hologram. A biprism is inserted in the illumination system and creates two coherent focussed beams at the specimen level with a probe size d= 5-10Å. If one beam passes through an object and another one passes in vacuum, an interference pattern, i.e. a hologram can be observed in diffraction plane (Fig.1).

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Neural computer analysis of chaotic dynamics of tremor parameters in different subjects' mental states under stimulation of auditory analyzer

PsycEXTRA Dataset ◽

10.1037/e573792014-010 ◽

2014 ◽

Author(s):

Valery Eskov ◽

Alexander Khadartsev ◽

Olga Filatova

Keyword(s):

Computer Analysis ◽

Chaotic Dynamics ◽

Mental States ◽

Auditory Analyzer ◽

Stimulation Of

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Biotelemetry and Computer Analysis of Electrocardiograms

Methods of Information in Medicine ◽

10.1055/s-0038-1635972 ◽

1969 ◽

Vol 08 (03) ◽

pp. 120-127 ◽

Cited By ~ 3

Author(s):

P. R. Amlinger

Keyword(s):

Computer Analysis ◽

Rural Areas ◽

Applied Research ◽

Research Effort ◽

Pilot Project ◽

Modern Medicine ◽

Modern Technology ◽

Long Distance ◽

Regional Medical ◽

Medical Program

Routine transmission of electrocardiograms and their computer interpretation via long-distance telephone lines has been proven feasible in the Automated Electrocardiogram Project of the Missouri Regional Medical Program. Though this Pilot Project — the first on a state-wide basis — is still viewed as an applied research effort rather than a service, such biotelemetry is rapidly gaining acceptance as a medium to bring modern medicine, through modern technology, to urban and remote rural areas as well, where it is most needed.The computer executes all the wave measuraments and calculations with incredible speed. It takes over a most boring, repetitive part of the physician’s work. However, it can only follow the instructions of the diagnostic program, compiled by expert cardiologists. Thus, it is an ever-ready, never-tiring servant for the physician and his patients.

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Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647943 ◽

1976 ◽

Vol 35 (01) ◽

pp. 186-190 ◽

Cited By ~ 6

Author(s):

Eugen A. Beck ◽

Peter Bachmann ◽

Peter Barbier ◽

Miha Furlan

Keyword(s):

Ionic Strength ◽

Factor Viii ◽

Gel Filtration ◽

High Ionic Strength ◽

Procoagulant Activity ◽

Carrier Protein ◽

Protease Inhibition ◽

Functional Sites ◽

Molecular Weight Peak ◽

Von Willebrand

SummaryAccording to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW~ 2 × 106) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37°C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macro molecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.

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