Simultaneous Determination of Ten Active Components in 12 Chinese Piper Species by HPLC

Piper is a genus that is recently valued for the treatment of central nervous system diseases. The major constituents, amides and lignans, are responsible for the antinociceptive and antidepressant activities. This study developed a RP-HPLC-UV method for the simultaneous determination of eight amides and two lignans in twelve different species of Piper. HPLC separation was accomplished on a C18 analytical column (5 μm, 250 mm × 4.6 mm, i.d.) with a gradient mobile phase consisting of acetonitrile and water at a flow rate of 1.0 ml/min. All the calibration curves showed good linear correlation coefficients (r > 0.9997) over the test ranges. The relative standard deviation of the current method was less than 2.90% for intra- and inter-day assays and the average recoveries were between 98.25% and 103.08%. The HPLC method established is appropriate for quality control purposes and allows for the differentiation of Piper species.

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The RP-HPLC method development and validation for simultaneous determination of oryzalin and ethofumesate pesticides in soil and water

Acta Chromatographica ◽

10.1556/1326.2021.00953 ◽

2021 ◽

Author(s):

Shishir Tandon ◽

Suman Lata Pal

Keyword(s):

Simultaneous Determination ◽

Method Development ◽

Hplc Method ◽

Limit Of Quantification ◽

Rp Hplc ◽

Relative Standard ◽

High Throughput Detection ◽

Hplc Method Development ◽

Soil And Water

Abstract A sensitive and reliable method for simultaneous determination of oryzalin and ethofumesate residues in pantnagar soil and water was validated. The compounds were extracted by LLE with dichloromethane from water, and acetone:methanol mixture from soil followed by SPE cleanup. Detection and quantification was done by RP-HPLC using mobile phase methanol:water (70:30, v/v) at 280 nm. The developed method showed satisfactory validation results with linearity (0.99), relative standard deviations (1.55 and 1.73%), and limit of quantification (0.002 μg g−1 and 0.005 μg g−1) for ethofumesate and oryzalin, respectively. Recoveries ranged for oryzalin and ethofumesate from 79.80–90.52, 75.58–86.04% (soil) and 83.50–95.92, 82.28–94.60% (water), respectively. The method could be used for routine high-throughput detection and determination of these compounds.

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Simultaneous Determination of L-Ascorbic Acid and L-Ascorbylpalmitate Using RP-HPLC Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.716.465 ◽

2013 ◽

Vol 716 ◽

pp. 465-469

Author(s):

Ye Lin Tian ◽

Jun Kai Wang ◽

Ping Sheng Leng ◽

Yun Liu

Keyword(s):

Ascorbic Acid ◽

Simultaneous Determination ◽

Methyl Palmitate ◽

High Performance ◽

Correlation Coefficients ◽

Reversed Phase ◽

Hplc Method ◽

Enzymatic Transesterification ◽

Rp Hplc

A rapid reversed phase highperformance liquid chromatography (RP-HPLC) method was developed for the simultaneous analysis of L-ascorbic acid and L-ascorbylpalmitate (AP). The chromatography was performed on a SSI model 2300-525 high performance liquid chromatographequipped with anAlltechApollo C18 column at 30 oC.The mobile phase was acetonitrile-water (90:10,v/v) with the flow rate of 1.0 mL/min. UV detection wavelength was 250 nm. This method permits the simultaneous determination of ascorbic acid and AP in the synthesis of AP transesterified with methyl palmitate and ascorbic acid. The detection limit of ascorbic acid and AP was 0.07μg/mL and 0.12μg/mL, respectively. The recovery was 90.59 ± 3.04% for ascorbic acid and 101.3 ± 4.81% for AP. The linearity range for ascorbic acid and AP was in the range of 0.1 - 0.7 mg/mL and 0.4 - 4.0 mg/mL, respectively. Correlation coefficients (R2) were 0.9910 for ascorbic acid and 0.9986 for AP. The proposed method could be used for routine quality control of AP synthesis with methyl palmitate and ascorbic acidby enzymatic transesterification.

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In Vitro Dissolution Study of Acetylsalicylic Acid and Clopidogrel Bisulfate Solid Dispersions: Validation of the RP-HPLC Method for Simultaneous Analysis

Applied Sciences ◽

10.3390/app10144792 ◽

2020 ◽

Vol 10 (14) ◽

pp. 4792 ◽

Cited By ~ 1

Author(s):

Ehlimana Osmanović Omerdić ◽

Larisa Alagić-Džambić ◽

Marko Krstić ◽

Maja Pašić-Kulenović ◽

Jadranka Odović ◽

...

Keyword(s):

Acetylsalicylic Acid ◽

Simultaneous Determination ◽

Solid Dispersions ◽

Hplc Method ◽

Poloxamer 407 ◽

In Vitro Dissolution ◽

Clopidogrel Bisulfate ◽

Rp Hplc ◽

Relative Standard

Solid dispersions were prepared via a solvent evaporation method, employing ethanol (96%, v/v) as solvent, with three different polymers as carrier: povidone, copovidone, and poloxamer 407. Previously developed reversed-phase HPLC (RP-HPLC) methods were modified and used for the simultaneous determination of acetylsalicylic acid and clopidogrel bisulfate and after release from solid dispersions. Chromatography was carried out on a C-18 column, with a mobile phase of acetonitrile–methanol–phosphate buffer pH 3.0, UV detection at 240 nm, and a run time of 6 min. The method was validated according to International Conference of Harmonisation guidelines and validation included specificity, accuracy, precision, linearity, robustness, limit of detection (LOD), and limit of quantification (LOQ). The method is specific for determination of acetylsalicylic acid and clopidogrel bisulfate. The linearity was provided in the concentration range 0.0275–0.1375 mg/mL for acetylsalicylic acid and 0.0200–0.1000 mg/mL for clopidogrel bisulfate, with a correlation coefficient (R2 value) of 0.9999 for both active pharmaceutical ingredients (APIs). Accuracy was confirmed by calculated recoveries for acetylsalicylic acid (98.6–101.0%) and clopidogrel bisulfate (100.0–101.6%). The intra-day and the inter-day precision-calculated relative standard deviations are less than 1%, which indicates high precision of the method. The limits of detection and quantification for acetylsalicylic acid were 0.0004 and 0.0012 mg/mL, and for clopidogrel bisulfate 0.0002 mg/mL and 0.0007 mg/mL, respectively. Small variations in chromatographic conditions did not significantly affect qualitative and quantitative system responses, which proved robustness of method. The proposed RP-HPLC method was applied for simultaneous determination of clopidogrel bisulfate and acetylsalicylic acid from solid dispersions.

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Validated Stability-Indicating RP-HPLC Method for the Determination of Norfloxacin in Pharmaceutical Dosage Form

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v10i4.873 ◽

2021 ◽

Vol 10 (4) ◽

Author(s):

Sirajunisa Talath ◽

Syeda Humaira

Keyword(s):

High Performance ◽

Correlation Coefficients ◽

Hplc Method ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Stability Indicating ◽

Rp Hplc ◽

Ich Guidelines ◽

Relative Standard

The objective of this work was to develop a simple, sensitive, accurate, precise and reproducible high performance liquid chromatography (HPLC) method for the determination of norfloxacin in pharmaceutical dosage forms. Shimadzo Prominance model L20 AD HPLC system equipped with SPD 20A UV-Vis detector was used for the analysis. The separation was done on RESTEX allure C18 column (3 μm, 15 cm × 4.6 mm), for an isocratic elution a mixture of methanol and water (60:40, v/v) mobile phase at a wavelength of 254 nm. The flow rate was 1.0 mL/min. The RP-HPLC method developed for analysis of norfloxacin was validated with respect to specificity, selectivity, linearity, accuracy, precision and robustness as per the ICH guidelines. The retention time of norfloxacin was 7.5 min. The linearity was established over the concentration ranges of 50-350 μg/mL with correlation coefficients ( r2) 0.999. The percentage accuracy of norfloxacin ranged from 99.76 -101.66%. The relative standard deviation values for intra-day and inter-day precision was lower than 2.0% and the assay result was found to be 100.65 %. Norfloxacin was subjected to stress conditions such as neutral, acidic, alkaline, oxidation and photolysis degradations as per ICH guidelines. The degradation studies revealed that the drug was found to degrade maximum (1.67%) in alkaline degradation conditions and was highly resistant towards neutral, acidic, oxidative and photolytic degradation conditions. Keywords: Norfloxacin, Validation, Stability-indicating, stress degradation, ICH guidelines.

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Stability-indicating RP-HPLC method for simultaneous determination of metformin hydrochloride and vildagliptin in tablet and biological samples

Acta Chromatographica ◽

10.1556/1326.2019.00555 ◽

2020 ◽

Vol 32 (1) ◽

pp. 39-43

Author(s):

Abdul Shakoor ◽

Mahmood Ahmed ◽

Rabia Ikram ◽

Sajad Hussain ◽

Arifa Tahir ◽

...

Keyword(s):

Simultaneous Determination ◽

Biological Samples ◽

Degradation Products ◽

Reversed Phase ◽

Hplc Method ◽

Coefficient Of Determination ◽

Metformin Hydrochloride ◽

Rp Hplc ◽

Relative Standard

The present work aimed to develop and validate a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of metformin hydrochloride and vildagliptin in tablet and biological samples. Isocratic elution of both the analytes was performed at 35 °C by injecting 20 μL into Thermo Hypersil ODS C18 column (5 μm, 4.6 mm× 250 mm), while the flow rate was set to 0.8 mL/min. The mobile phase comprised of methanol, acetonitrile, and phosphate buffer (5:30:65, v/v, pH 3.5), and wavelength was selected at 212 nm. The overall run time per sample was 7.0 min with a retention time of 3.36 and 5.41 min for metformin hydrochloride and vildagliptin, respectively. The calibration curve was linear from 10–140 μg/mL for metformin and 1–14 μg/mL for vildagliptin with a coefficient of determination (R2) ≤ 0.9919, while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.13 and 0.97%, respectively. Force degradation studies indicated a complete separation of the analytes in the presence of their degradation products providing a high degree of method specificity. The proposed reversed-phase high-performance liquid chromatography (RP-HPLC) method was demonstrated to be simple and rapid for the determination of metformin hydrochloride and vildagliptin in commercially available tablet and biological samples providing recoveries ranged between 100.13–100.29%.

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Development and Validation of a RP-HPLC Method for Simultaneous Determination of Levofloxacin and Moxifloxacin in Pharmaceutical Dosage Forms

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.17614 ◽

2014 ◽

Vol 12 (2) ◽

Author(s):

Farjahan Nur ◽

Asma Rahman ◽

Md. Zakir Sultan ◽

Md. Gias Uddin ◽

Mohammad A Rashid

Keyword(s):

Simultaneous Determination ◽

Dosage Forms ◽

Hplc Method ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc ◽

Development And Validation

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RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

International Journal of Scientific Research ◽

10.15373/22778179/feb2013/123 ◽

2012 ◽

Vol 2 (2) ◽

pp. 364-367 ◽

Cited By ~ 1

Author(s):

Saida Naik Dheeravath ◽

◽

Kasani Ramadevi ◽

Zilla Saraswathi ◽

Dheeravath Maniklal ◽

...

Keyword(s):

Simultaneous Determination ◽

Method Development ◽

Hplc Method ◽

Rp Hplc ◽

Hplc Method Development

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Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

Journal of Chromatographic Science ◽

10.1093/chromsci/bmab018 ◽

2021 ◽

Author(s):

Hina Shamshad ◽

Ali Sayqal ◽

Jahan Zeb ◽

Agha Zeeshan Mirza

Keyword(s):

Human Serum ◽

Simultaneous Determination ◽

Active Form ◽

Internal Standard ◽

Hplc Method ◽

Serum Samples ◽

Rp Hplc ◽

Cetirizine Hydrochloride ◽

Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

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