Myrcene, an Aromatic Volatile Compound, Ameliorates Human Skin Extrinsic Aging via Regulation of MMPs Production

Myrcene is an aromatic volatile compound that is commercially well-known as a flavor ingredient in the food industry and a fragrance in the soap and detergent industry. Given the worldwide interest in natural antiphotoaging products, we investigated the protective effects of myrcene in UVB-irradiated human dermal fibroblasts (NHDFs). NHDFs were subjected to 144[Formula: see text]mJ/cm2of UVB irradiation. The expression of intracellular reactive oxygen species (ROS), matrix metalloproteinase-1 (MMP-1), MMP-3, interleukin-6 (IL-6), transforming growth factor (TGF-[Formula: see text]1) and type I procollagen were examined. We showed that myrcene decreased the production of ROS, MMP-1, MMP-3, and IL-6, and increased TGF-[Formula: see text]1 and type I procollagen secretions. Furthermore, myrcene treatment (0.1–10[Formula: see text][Formula: see text]M) dramatically reduced the activation of MAPK-related signaling molecules such as p-ERK, p-p38, and p-JNK and AP-1 including p-c-Jun and p-c-Fos. Our data indicate that myrcene has a potential protective effect on UVB-induced human skin photoaging. Therefore, myrcene might have applications in the skincare industry.

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Protective Effects of Indole 3-Acetonitrile-4-Methoxy-2-S-β-d-Glucopyranoside From Nasturtium officinale R. Br. Against Ultraviolet B-Induced Photodamage in Normal Human Dermal Fibroblasts

Natural Product Communications ◽

10.1177/1934578x19872425 ◽

2019 ◽

Vol 14 (8) ◽

pp. 1934578X1987242

Author(s):

Yumin Kim ◽

Kyung Suk Bae

Keyword(s):

Dermal Fibroblasts ◽

Ultraviolet B ◽

Type I ◽

Protective Effects ◽

Human Dermal Fibroblasts ◽

Nasturtium Officinale ◽

Type I Procollagen ◽

Matrix Metalloproteinase 1 ◽

Normal Human ◽

Normal Human Dermal Fibroblasts

Ultraviolet radiation induces skin photoaging, which is associated with the elevation of matrix metalloproteinase-1 (MMP-1) and the decrease of procollagen. Nasturtium officinale plays a well-known role in the treatment of sulfur-containing compounds and their important role in protecting human health. However, their skin protective activity toward UVB-induced photodamage remains unclear. In the present study, we investigated the protective effect of indole 3-acetonitrile-4-methoxy-2- S-β-d-glucopyranoside (IAMG) from N. officinale on UVB-irradiated normal human dermal fibroblasts (NHDF). Our results show that IAMG enhanced NHDF cell migration. The UVB-induced increases in MMP-1 and decrease in type I procollagen were ameliorated by IAMG treatment. Taken together, our data strongly suggest that IAMG from N. officinale could reduce UVB-induced photodamage.

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Antiphotoaging Potential of Extracts of Yin-Tonic Herbal Medicine in Skin Cell and Human Skin Equivalent

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/8881270 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Yun-mi Kang ◽

Min-gyu Seo ◽

Kyou-young Lee ◽

Hyo-jin An

Keyword(s):

Human Skin ◽

Herbal Medicines ◽

The Body ◽

Type I ◽

Skin Damage ◽

Skin Equivalents ◽

Type I Procollagen ◽

Matrix Metalloproteinase 1 ◽

Dermal Thickness ◽

Skin Photoaging

Yin-tonic herbal medicines have been shown to possess properties that make skin healthy by nourishing within various organs of the body. However, the antiphotoaging effect of these medicines on the skin has not been fully studied. Photoaging occurs with prolonged sun exposure and causes skin damage and aging, with depletion of the dermal extracellular matrix and chronic alterations in skin structure, such as wrinkles. In this study, we assessed the antiphotoaging effects of eight yin-tonic herbal medicines on human skin cells and skin equivalents. The levels of type I procollagen and matrix metalloproteinase-1 (MMP-1) in ultraviolet B- (UVB-) irradiated CCD-986sk fibroblasts were measured, and then three medicines were chosen based on screening results. Using UVB-irradiated human skin equivalents, we evaluated the effect of three yin-tonic herbal medicines on histological changes of skin, epidermal and dermal thickness, and MMP-1 production. Furthermore, we observed collagen fiber content and protein expression of filaggrin in UVB-irradiated human skin equivalents. Yin-tonic herbal medicines increased type I procollagen levels and decreased the production of MMP-1 in UVB-irradiated CCD-986sk fibroblasts. The three selected yin-tonic herbal medicines recovered the collagen content and filaggrin expression via MMP-1 downregulation in UVB-irradiated human skin equivalents. Our results show that yin-tonic herbal medicines can prevent skin photoaging by reduction of MMP-1 levels and increasing the expression of moisturizing factors. Based on these results, we suggest that yin-tonic herbal medicines have the potential to be used as helpful agent for skin photoaging.

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Collagen matrices attenuate the collagen-synthetic response of cultured fibroblasts to TGF-beta

Journal of Cell Science ◽

10.1242/jcs.108.3.1251 ◽

1995 ◽

Vol 108 (3) ◽

pp. 1251-1261 ◽

Cited By ~ 9

Author(s):

R.A. Clark ◽

L.D. Nielsen ◽

M.P. Welch ◽

J.M. McPherson

Keyword(s):

Growth Factor ◽

Granulation Tissue ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Collagen Matrix ◽

Dermal Fibroblasts ◽

Type I ◽

Collagen Gels ◽

Type I Procollagen ◽

Growth Factor Beta

Transforming growth factor-beta, a potent modulator of cell function, induces fibroblasts cultured on plastic to increase collagen synthesis. In 5- and 7-day porcine skin wounds, which have minimal to moderate collagen matrix, respectively, transforming growth factor-beta and type I procollagen were coordinately expressed throughout the granulation tissue. However, in 10-day collagen-rich granulation tissue type I procollagen expression diminished despite persistence of transforming growth factor-beta. To investigate whether collagen matrix attenuates the collagen-synthetic response of fibroblasts to transforming growth factor-beta, we cultured human dermal fibroblasts in conditions that simulate collagen-rich granulation tissue. Therefore, human dermal fibroblasts were suspended in attached collagen gels and collagen and noncollagen production was assayed in the absence and presence of transforming growth factor-beta. Although transforming growth factor-beta stimulated collagen synthesis by fibroblasts cultured in the collagen gels, these fibroblasts consistently produced less collagen than similarly treated fibroblasts cultured on plastic. This phenomenon was not secondary to nonspecific binding of transforming growth factor-beta to the collagen matrix. Fibroblasts cultured in a fibrin gel responded to transforming growth factor-beta similarly to fibroblasts cultured on plastic. Using immunofluorescence probes to type I procollagen, we observed that transforming growth factor-beta increased type I procollagen expression in most fibroblasts cultured on plastic, but only in occasional fibroblasts cultured in collagen gels. From these data we conclude that collagen matrices attenuate the collagen synthetic response of fibroblast to transforming growth factor-beta in vitro and possibly in vivo.

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The effect of narrowband ultraviolet B on the expression of matrix metalloproteinase-1, transforming growth factor-?1 and type I collagen in human skin fibroblasts

Clinical and Experimental Dermatology ◽

10.1111/j.1365-2230.2006.02309.x ◽

2007 ◽

Vol 32 (2) ◽

pp. 180-185 ◽

Cited By ~ 28

Author(s):

C. P. Choi ◽

Y. I. Kim ◽

J. W. Lee ◽

M. H. Lee

Keyword(s):

Growth Factor ◽

Matrix Metalloproteinase ◽

Human Skin ◽

Type I Collagen ◽

Transforming Growth Factor ◽

Ultraviolet B ◽

Skin Fibroblasts ◽

Type I ◽

Human Skin Fibroblasts ◽

Matrix Metalloproteinase 1

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Effects of Tenascin C on the Integrity of Extracellular Matrix and Skin Aging

International Journal of Molecular Sciences ◽

10.3390/ijms21228693 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8693

Author(s):

Young Eun Choi ◽

Min Ji Song ◽

Mari Hara ◽

Kyoko Imanaka-Yoshida ◽

Dong Hun Lee ◽

...

Keyword(s):

Extracellular Matrix ◽

Type I Collagen ◽

Transforming Growth Factor ◽

Mrna Level ◽

Skin Aging ◽

Dermal Fibroblasts ◽

Cell Physiology ◽

Type I ◽

Tenascin C ◽

Matrix Metalloproteinase 1

Tenascin C (TNC) is an element of the extracellular matrix (ECM) of various tissues, including the skin, and is involved in modulating ECM integrity and cell physiology. Although skin aging is apparently associated with changes in the ECM, little is known about the role of TNC in skin aging. In this study, we found that the Tnc mRNA level was significantly reduced in the skin tissues of aged mice compared with young mice, consistent with reduced TNC protein expression in aged human skin. TNC-large (TNC-L; 330-kDa) and -small (TNC-S; 240-kDa) polypeptides were observed in conditional media from primary dermal fibroblasts. Both recombinant TNC polypeptides, corresponding to TNC-L and TNC-S, increased the expression of type I collagen and reduced the expression of matrix metalloproteinase-1 in fibroblasts. Treatment of fibroblasts with a recombinant TNC polypeptide, corresponding to TNC-L, induced phosphorylation of SMAD2 and SMAD3. TNC increased the level of transforming growth factor-β1 (TGF-β1) mRNA and upregulated the expression of type I collagen by activating the TGF-β signaling pathway. In addition, TNC also promoted the expression of type I collagen in fibroblasts embedded in a three-dimensional collagen matrix. Our findings suggest that TNC contributes to the integrity of ECM in young skin and to prevention of skin aging.

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The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/2576823 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13 ◽

Cited By ~ 2

Author(s):

You-Cheng Hseu ◽

Yugandhar Vudhya Gowrisankar ◽

Xuan-Zao Chen ◽

Yi-Chen Yang ◽

Hsin-Ling Yang

Keyword(s):

Human Skin ◽

Food Supplement ◽

Skin Aging ◽

Dermal Fibroblasts ◽

Ros Generation ◽

Human Skin Fibroblast ◽

Type I ◽

Dependent Manner ◽

Protective Effects ◽

Antioxidant Genes

UVA irradiation induced ROS-mediated photo damage to the human skin leading to coarseness, wrinkling, pigmentation, and cutaneous malignancies. We investigated the dermatoprotective efficacies of submicromolar concentrations of ergothioneine (EGT, 0.125-0.5 μM), which occurs naturally as a sulfur-containing amino acid, in the mechanisms in human skin fibroblast (HSF) cells. UVA-induced AP-1 (c-Fos and c-Jun) translocation was found to be inhibited by EGT treatments with the parallel inhibition of the collagenolytic matrix metalloproteinase- (MMP-) 1 activation and type I procollagen degradation. Moreover, EGT mitigated UVA-induced ROS generation. An increase in the amount of antioxidant genes (HO-1, NQO-1, and γ-GCLC) from EGT and were associated with upregulated Nrf2 expressions in a dose-dependent or time-dependent manner. We confirmed this from Nrf2 translocation and increased nuclear ARE promoter activity that underlie EGT dermatoprotective activities. Also, glutathione (GSH) levels (from γ-GCLC) were significantly increased. Moreover, we showed that mediated by ERK, JNK, and PKC, signaling cascades mediate Nrf2 translocation. We confirmed this phenomenon by the suppressed nuclear Nrf2 activation in cells that were treated with respective inhibitors (PD98059, SP600125, and GF109203X). However, antioxidant protein expressions were impaired in Nrf2 knockdown cells to confirm that ARE/Nrf2 pathways and the inhibition of AP-1 had significant roles in EGT-mediated protective effects. We can conclude that ergothioneine ameliorated UVA-induced skin aging and is a useful food supplement for skin care products.

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The effect of cilostazol on the expression of matrix metalloproteinase-1 and type I procollagen in ultraviolet-irradiated human dermal fibroblasts

Life Sciences ◽

10.1016/j.lfs.2012.12.011 ◽

2013 ◽

Vol 92 (4-5) ◽

pp. 282-288 ◽

Cited By ~ 11

Author(s):

Byeng Chul Yu ◽

Dae-Sung Lee ◽

Sang Mo Bae ◽

Won-Kyo Jung ◽

Jin Ho Chun ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Dermal Fibroblasts ◽

Type I ◽

Human Dermal Fibroblasts ◽

Type I Procollagen ◽

Matrix Metalloproteinase 1

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Stress-relaxation and contraction of a collagen matrix induces expression of TGF-β and triggers apoptosis in dermal fibroblasts

Biochemistry and Cell Biology ◽

10.1139/o00-014 ◽

2000 ◽

Vol 78 (4) ◽

pp. 427-436 ◽

Cited By ~ 10

Author(s):

M Varedi ◽

E E Tredget ◽

A Ghahary ◽

P G Scott

Keyword(s):

Mechanical Properties ◽

Growth Factor ◽

Type I Collagen ◽

Transforming Growth Factor ◽

Collagen Matrix ◽

Dermal Fibroblasts ◽

Type I ◽

Collagen Matrices ◽

Matrix Metalloproteinase 1 ◽

Tgf Β1

Extracellular matrix serves as a scaffold for cells and can also regulate gene expression and ultimately cell behaviour. In this study, we compared the effects of three forms of type I collagen matrix, which differed only in their mechanical properties, and plastic on the expression of transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-1 (collagenase), and type I collagen and on the growth and survival of human dermal fibroblasts. These effects were correlated with alterations in cell morphology and organization of intracellular actin. Cells in detached or stress-relaxed matrices were spherical, lacked stress fibres, and showed increased TGF-β1 mRNA compared to the cells in anchored collagen matrices or on plastic, which were polygonal or bipolar and formed stress fibres. The levels of TGF-β measured by bioassay were higher in detached and stress-relaxed collagen matrices, than in anchored collagen matrices. Cells on plastic contained little or no immunoreactive TGF-β, while most cells in collagen matrices were stained. The levels of collagenase mRNA were significantly higher in all the collagen matrix cultures compared to those on plastic, but there were no statistically significant differences between them. Levels of mRNA for procollagen type I were not significantly affected by culture in the collagen matrices. Apoptotic fibroblasts were detected by the TUNEL assay in detached (5.7%) and to a lesser extent in stress-relaxed (2.2%) matrices, but none were observed in anchored collagen matrices or on plastic. These results show that alterations in the mechanical properties of matrix can induce the expression of TGF-β and trigger apoptosis in dermal fibroblasts. They further suggest that inability to reorganize this matrix could be responsible for the maintenance of the fibroproliferative phenotype associated with fibroblasts in hypertrophic scarring. Key words: transforming growth factor-β, apoptosis, fibroblasts.

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