Effect of Commiphora Molmol (Oleo-gum-resin) on the Cytological and Biochemical Changes Induced by Cyclophosphamide in Mice

1994 ◽  
Vol 22 (01) ◽  
pp. 77-82 ◽  
Author(s):  
M.M. AI-Harbi ◽  
S. Qureshi ◽  
M.M. Ahmed ◽  
S. Rafatullah ◽  
A.H. Shah
Keyword(s):  
Nucleic Acids ◽  
Biochemical Changes ◽  
Additive Effect ◽  
Swiss Albino Mice ◽  
Hepatic Cells ◽  
Albino Mice ◽  
Dose Dependent ◽  
Rna Levels

The anticlastogenic and biochemical potentials of Commiphora molmol were studied in Swiss albino mice treated with cyclophosphamide (CP). The C. molmol treatment (125-500 mg/kg) showed no mutagenicity. It caused a highly significant and dose-dependent mitodepressant effect in the femoral cells and reduction of RNA levels in hepatic cells as compared with the control. CP treatment showed significant increase in the frequency of micronuclei, cytotoxicity and reduction in the contents of nucleic acids and proteins. Pretreatment with C. molmol could neither alter the biochemical and cytological effects of CP nor show any additive effect of both treatments.

scholarly journals Effect of Poly (N-IsoPropylAcrylamide) “PNIPAM” on Hepatic Cells of Swiss Albino Mice, Mus musculus

2013 ◽  
Vol 1 (4) ◽  
pp. 208-213 ◽  
Author(s):  
Md. Imtiyaz Aslam ◽  
Rohit Kumar Verma ◽  
Richa Roy ◽  
S. P. Roy
Keyword(s):  
Aqueous Solution ◽  
Mus Musculus ◽  
Quantitative Estimation ◽  
Polymeric Compound ◽  
Swiss Albino Mice ◽  
Hepatic Cells ◽  
Isopropyl Acrylamide ◽  
Albino Mice ◽  
Xenobiotic Compound ◽  
Histological Result

The present paper deals with the effect of polymeric compound poly (N-isopropyl acrylamide) “PNIPAM” for their toxicity on hepatic cells. The nanoparticle is a xenobiotic compound that accumulates in the liver for their metabolism. Non-metabolizing xenobiotic compounds such as PNIPAM produces anomalies in the hepatic cells. Certain enzymes such as ALT and ALP can be assayed for the hepatocytic toxicity.An attempt has been made to know the toxic effect of “PNIPAM” in a concentration of 0.8mg/ml on the hepatic cells of Swiss Albino mice, Mus musculus. The ALT and ALP analysis were performed through test kits for their quantitative estimation. The histological result shows that several lesions were produced after the introduction of aqueous solution of PNIPAM for an incubation period of 48 hours. The toxicity was confirmed Spectrophotometrically by the assessment of enzyme ALT and ALP. The increased concentration of ALT (55.0 IU/L) and slight decrease in ALP (40.0 IU/L) concentration was responsible for the metabolic alteration and production of hepatocytic anomalies in the mice.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9104    Int J Appl Sci Biotechnol, Vol. 1(4): 208-213

scholarly journals Antiarthritic Activity of Achyranthes Aspera on Formaldehyde - Induced Arthritis in Rats

2019 ◽  
Vol 7 (17) ◽  
pp. 2709-2714
Author(s):  
Velmurugan Chinnasamy ◽  
Vetriselvan Subramaniyan ◽  
Sarath Chandiran ◽  
Saminathan Kayarohanam ◽  
Dhanapal Сhidambaram Kanniyan ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Test Dose ◽  
Joint Inflammation ◽  
Negative Control ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
C 50 ◽  
Dose Dependent ◽  
Histopathological Investigation

AIM: To determine the ameliorative potential of aqueous extract of Achyranthesaspera(AEAA) against arthritis using swiss albino mice and Wistar rats, and its possible mechanism of action. METHODS: Swiss albino mice (25-30 g) and Wistar rats (150-180 g) under standard controlled conditions (24 ± 2°C, 50-70 humidity and 12 h light/dark cycle). The groups were divided into 6 groups (n = 6/group) and assigned as control, negative control, standard and, formaldehyde supplemented with two different test dose groups of A. aspera for 4 weeks. Arthritis induced by subplantar administration of 0.1 ml formaldehyde (2% v/v) into the left hind paw in all groups except normal control. Arthritis was assessed using serum Hb, ESR, paw volume, joint diameter, radiological and histopathological investigation. RESULTS: Oral administration of AEAA shown a significant (p < 0.01) dose-dependent protection against formaldehyde induced arthritis. At 21st day, A.asperashown an inhibition of paw volume in the different doses of 250 mg/kg and 500 mg/kg were found to be 30% and, 38.33% respectively. At 14th day the joint swelling was found to be 27.2% and 36.36 respectively. Diclofenac (10 mg/kg) had an effect of 36.61% inhibition of arthritis and joint swelling at 21st and 14th day. CONCLUSION: Thus, the present study revealed that the aqueous extract of A. aspera offered significant protection against arthritis and joint inflammation.

scholarly journals Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Oluyinka A Iyiola ◽  
Temitope F. Olafimihan
Keyword(s):  
Silver Nanoparticles ◽  
Portal Tract ◽  
Sperm Head ◽  
Mouse Bone Marrow ◽  
Total N ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Head Morphology ◽  
Dose Dependent ◽  
Positive Controls

Silver nanoparticles (AgNPs) are widely used in industrial and medical applications. However, there is a growing concern about the potentialities of AgNPs to induce genotoxicity and DNA damage in humans. In this study, genotoxic and histopathological effects of AgNPs were investigated in mice using two well-characterized genetic assays: mouse bone marrow micronuclei (MN) and mouse sperm morphology assays. Swiss albino mice (total N=18) were exposed to varying concentrations (3,000mg/Kg, 4,000mg/Kg, 5,000mg/Kg and 6,000mg/Kg) of AgNPs for 5 consecutive days and observed for 30 days afterwards. Distilled water and colchicine were used as negative and positive controls, respectively. The MN assay showed that the frequency of micronuclei induction increased with AgNP concentration. Statistically significant differences (p<0,05) were observed for the micronucleus frequency in the blood erythrocytes in all the test concentrations. Sperm head morphology assay also revealed various types of abnormal sperm head morphology and there was statistically significant increase in frequency of sperm abnormalities. Histopathological profiles of the liver also showed enlarge sinusoids, irregular portal tract, and dose-dependent vacuolation. These results suggest that AgNPs is genotoxic and represent a serious health risk to human heatlh.

Dose-dependent suppression of haloperidol-induced catalepsy by potentized Agaricus muscarius

1996 ◽  
Vol 85 (03) ◽  
pp. 141-144 ◽  
Author(s):  
Souvik Ghosh ◽  
S.P. Sinha Babu ◽  
N.C. Sukul
Keyword(s):  
Treatment Effect ◽  
Post Treatment ◽  
Active Principle ◽  
Mechanical Agitation ◽  
Swiss Albino Mice ◽  
Before And After ◽  
Albino Mice ◽  
Pre Treatment ◽  
Dose Dependent ◽  
Post Treatment Effect

Abstract Agaricus muscarius 30c, a potentized homoeopathic drug prepared by successive dilution with 90% alcohol followed by sonication, suppressed haloperidol-induced catalepsy in Swiss albino mice significantly. This anticataleptic effect was dose-dependent being greatest with the undiluted Agaricus 30c and least if diluted 1:20,000. Higher dilutions like 1:40,000 and 1:50,000 did not produce an anticataleptic effect. The effect reappeared when the 1:50,000 dilution was sonicated. The anticataleptic effect of potentized Agaricus was observed with the drug administered both before and after haloperidol. However, the pre-treatment effect was more pronounced than the post-treatment effect.It is thought that potentized Agaricus contains an active principle that can be attenuated by dilution and multiplied by mechanical agitation or sonication.

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