Unsei-in Inhibits Rheological Activity of Leukocytes: Mechanism of Action in Neutrophil-related Skin Diseases

1995 ◽  
Vol 23 (01) ◽  
pp. 81-90 ◽  
Author(s):  
Shigeruko lijima ◽  
Fujio Otsuka ◽  
Yuji Kikuchi
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Skin Diseases ◽  
Behçet's Disease ◽  
Statistical Significance ◽  
Inhibitory Effect ◽  
Filtration Method ◽  
Behcet's Disease ◽  
Normal Individuals

To investigate the efficacy of Unsei-in on skin diseases such as Behçet's disease, palmoplantar pustulosis and psoriasis vulgaris, the rheological activity of leukocytes as manifested by adhesiveness, deformability and aggregability was studied in vitro using a micropore filtration method. Unsei-in inhibited the rheological activity of leukocytes at a statistical significance of p < 0.01 in normal individuals whose leukocytes had been stimulated with N-formyl-methionyl-leucyl-phenylalanine (FMLP). It did not, however, inhibit the activity of cells that had not been stimulated by FMLP. In contrast, in Behçet's disease, Unsei-in inhibited rheological activity of non-stimulated leukocytes ( p < 0.01) but did not inhibit the rheological activity of FMLP-stimulated leukocytes. In a study using Ouren-gedoku-to and Shimotsu-to, the main constituents of Unsei-in, the latter inhibited the activity of FMLP-stimulated leukocytes in normal individuals ( p < 0.02). These results indicate that Unsei-in has an inhibitory effect on rheologically-activated neutrophils, which are involved in the healing mechanisms of the above skin diseases, and it also has a satisfactory effect in the form of a mixture of Shimotsu-to and Ouren-gedoku-to.

scholarly journals Different Methylation of CpG-SNPs in Behcet’s Disease

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Yang Huang ◽  
Handan Tan ◽  
Qingfeng Cao ◽  
Gangxiang Yuan ◽  
Guannan Su ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Genetic Variants ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Association Studies ◽  
Statistical Significance ◽  
Multiple Comparisons ◽  
Healthy Individuals ◽  
Behcet's Disease ◽  
Second Stage

Purpose. We recently performed an Epigenome-Wide Association Studies (EWAS) study in Behcet’s disease (BD) and identified various cytosine–phosphate–guanine (CpG) loci that were aberrantly methylated. In the current study, we wanted to investigate whether these sites contained genetic polymorphisms and whether the frequency of these polymorphisms was altered in BD.Methods. A two-stage study was performed. The first stage involved 358 BD patients and 704 healthy controls to investigate genetic variants of 10 CpG-SNPs (rs10454134, rs176249, rs3808620, rs10176517, rs11247118, rs78016579, rs9461624, rs10492166, rs34929465, and rs6507921) using an iPLEX Gold genotyping assay and a Sequenom MassARRAY. In the second stage, an additional 172 independent BD patients and 330 healthy individuals are to confirm trends found in the first stage.Results. A higher frequency of both the rs10454134 AG genotypes (p = 0.008, OR = 1.413, 95% CI = 1.094-1.826) and a lower GG genotype frequency (p = 0.003, OR = 0.630, 95% CI = 0.465-0.854) were found in BD patients compared to the controls in the first stage. However, after correcting for multiple comparisons, all associations identified in the first stage lost statistical significance. The frequencies of the other CpG-SNPs investigated were not different between BD patients and controls. The second stage was designed using an additional cohort to confirm the association with CpG-SNP, rs10454134. The data failed to confirm the association between this CpG-SNP and BD.Conclusions. This study did not show an association between BD and CpG-SNPs in gene sites that were earlier shown to be aberrantly methylated.

Serum of Patients with Behçet’s Disease Induces Classical (Pro-Inflammatory) Activation of Human Macrophages in vitro

Dermatology ◽  
2003 ◽  
Vol 206 (3) ◽  
pp. 225-232 ◽  
Author(s):  
E. Alpsoy ◽  
V. Kodelja ◽  
S. Goerdt ◽  
C.E. Orfanos ◽  
Ch.C. Zouboulis
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Behcet's Disease ◽  
Human Macrophages ◽  
Inflammatory Activation

In vitro evaluation of γδ T cells regulatory function in Behçet’s disease patients and healthy controls

2016 ◽  
Vol 77 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Antonio Clemente Ximenis ◽  
Catalina Crespí Bestard ◽  
Ana Cambra Conejero ◽  
Lucio Pallarés Ferreres ◽  
Antonio Juan Mas ◽  
...  
Keyword(s):  
T Cells ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Γδ T Cells ◽  
Regulatory Function ◽  
Healthy Controls ◽  
In Vitro Evaluation ◽  
Behcet's Disease

scholarly journals The Evaluation of the Relationship between sTREM-1, VEGF-B, and VEGF Gene Expression Levels with Disease Activity of Behçet’s Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Kadir Harmanci ◽  
Ozden Yildirim Akan ◽  
Timur Pirildar ◽  
Pinar Ortan ◽  
Cevval Ulman
Keyword(s):  
Gene Expression ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Statistical Significance ◽  
Vascular Involvement ◽  
Control Group ◽  
Vegf Gene ◽  
Gene Expressions ◽  
Behcet's Disease

Background. There is no specific marker that shows the disease activity in Behçet’s disease. Aim. In this study, we aimed to investigate VEGF-B and VEGF gene expressions and sTREM-1 levels in association with the activation of Behçet’s disease. Study Design. Case-control study. Methods. Clinical features of patients who applied in the rheumatology clinic and were diagnosed with BD according to the international working group’s criteria were investigated. 30 healthy volunteers and 30 patients in the active period according to the EBDCAF scoring were studied. VEGF-B and VEGF gene expressions and sTREM-1 levels were studied in the serum samples of the patients and the control subjects. Results. The VEGF-B expressions and sTREM-1 levels were higher in the BD than those in the healthy group, but this difference did not reach statistical significance. VEGF gene expression was statistically significant (p=0.008). Behçet’s disease patients with oral aphthae, genital ulcer, eye, joint, vascular, skin, and neurological involvement were analyzed separately as subgroups. We find that VEGF gene expression level of Behçet’s disease patients with joint involvement (arthritis/arthralgia) and also VEGF-B and VEGF gene expression of Behçet’s disease with vascular involvement (DVT/thrombophlebitis) were significantly higher (p=0.035, p=0.021). Each subgroup was analyzed with the control group. We determined that VEGF gene expression in all subgroups was significantly higher than that in the control group. At the same time, VEGF-B levels of patients with genital ulcer and vascular involvement (DVT/thrombophlebitis) were significantly higher than those in the control group. Conclusion. VEGF-B and VEGF gene expressions can be activity indicators for BD. In addition, this study shows that new treatment options should be explored for Behçet’s disease patients with joint and vascular involvement. In the following years, new treatment methods are needed to investigate for revealing the role of the etiopathogenesis of BD and the activation and prognosis of VEGF by examining this study and providing much more participation. In our study group, the sTREM-1 levels were high but the results did not reach statistical significance. More studies are needed with larger groups in order the highlight the exact role of STREM-1 in Behçet’s disease.

scholarly journals Glucocorticoid sensitivity in Behçet's disease

2012 ◽  
Vol 1 (2) ◽  
pp. 103-111 ◽  
Author(s):  
R A M Quax ◽  
J A M van Laar ◽  
R van Heerebeek ◽  
K Greiner ◽  
E Ben-Chetrit ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Behçet’S Disease ◽  
Gene Polymorphisms ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Healthy Controls ◽  
Behcet's Disease ◽  
Expression Levels ◽  
Mrna Expression Levels

ObjectiveGlucocorticoid (GC) sensitivity is highly variable among individuals and has been associated with susceptibility to develop (auto-)inflammatory disorders. The purpose of the study was to assess GC sensitivity in Behçet's disease (BD) by studying the distribution of four GC receptor (GR) gene polymorphisms and by measuring in vitro cellular GC sensitivity.MethodsHealthy controls and patients with BD in three independent cohorts were genotyped for four functional GR gene polymorphisms. To gain insight into functional differences in in vitro GC sensitivity, 19 patients with BD were studied using two bioassays and a whole-cell dexamethasone-binding assay. Finally, mRNA expression levels of GR splice variants (GR-α and GR-β) were measured.ResultsHealthy controls and BD patients in the three separate cohorts had similar distributions of the four GR polymorphisms. The Bcll and 9β minor alleles frequency differed significantly between Caucasians and Mideast and Turkish individuals.At the functional level, a decreased in vitro cellular GC sensitivity was observed. GR number in peripheral blood mononuclear cells was higher in BD compared with controls. The ratio of GR-α/GR-β mRNA expression levels was significantly lower in BD.ConclusionsPolymorphisms in the GR gene are not associated with susceptibility to BD. However, in vitro cellular GC sensitivity is decreased in BD, possibly mediated by a relative higher expression of the dominant negative GR-β splice variant. This decreased in vitro GC sensitivity might play an as yet unidentified role in the pathophysiology of BD.

Pentoxifylline Inhibits Vγ9/Vδ2 T Lymphocyte Activation of Patients with Active Behçet's Disease in Vitro

2007 ◽  
Vol 20 (3) ◽  
pp. 601-606 ◽  
Author(s):  
A. Accardo-Palumbo ◽  
A. Ferrante ◽  
F. Ciccia ◽  
M. Cadelo ◽  
A.R. Giardina ◽  
...  
Keyword(s):  
T Cell ◽  
Behçet’S Disease ◽  
Cell Expansion ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Receptor Expression ◽  
Lymphocyte Function ◽  
Behcet's Disease ◽  
Vitro Effect

The aim of this study is to evaluate the in vitro effect of pentoxifylline (PTX) on T Vγ9/Vδ2 lymphocyte function in Behçet's disease (BD). We investigated the effect of PTX on Vγ9/Vδ2 T cell expansion and expression of TNFRII receptor and perforin content before and after PTX addition by means of FACS analysis lymphocyte cultures from patients with active and inactive BD and healthy subjects. The addition of PTX at a concentration of 1 mg/ml determined a significant inhibition of cell expansion, a down regulation of TNF receptor expression and inhibited the PMA-induced degranulation of perforin. Taken together these data indicate that PTX is capable of interfering with Vγ9/Vδ2 T cell function in BD, and although cell culture models cannot reliably predict all of the potential effects of the drug in vivo, our results encourage the possibility that this drug may find use in a range of immunological disorder characterized by dysregulated cell-mediated immunity.

Effects of in vivo and in vitro administration of neuro-Behcet’s disease IgG

2017 ◽  
Vol 38 (5) ◽  
pp. 833-843 ◽  
Author(s):  
Ece Erdağ ◽  
Ceren Şahin ◽  
Cem İsmail Küçükali ◽  
Sinem Bireller ◽  
Melike Küçükerden ◽  
...  
Keyword(s):  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Behcet's Disease
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