Anti-inflammatory Effects of Mao-Bushi-Saishin-to in Mice and Rats

1998 ◽  
Vol 26 (02) ◽  
pp. 171-179 ◽  
Author(s):  
Yoshiki Ikeda ◽  
Osamu T. Iijima ◽  
Akira lizuka ◽  
Atsushi Ishige ◽  
Sakae Amagaya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Arachidonic Acid ◽  
Mast Cells ◽  
Phorbol Ester ◽  
Underlying Mechanism ◽  
Tissue Growth ◽  
Cotton Pellet ◽  
Hind Foot ◽  
Anti Inflammatory ◽  
Permeability Increase

Effects of Mao-Bushi-Saishin-to (MBS) on anti-inflammatory activities were examined in mice and rats. MBS significantly inhibited the increase in vascular permeability induced by acetic acid, the ear edema induced by arachidonic acid and phorbol ester, and the cutaneous extravasation induced by bradykinin and histamine. MBS, however, was not effective against the serotonin-induced cutaneous permeability increase in mice. MBS significantly inhibited carrageenin-induced hind foot edema and cotton pellet-induced granulation tissue growth in rats. These results show that MBS may exert anti-inflammatory effects through the underlying mechanism(s) of preventing mediator release from mast cells and macrophages.

scholarly journals “EVALUATION OF GALPHIMIA GLAUCA STEM METHANOL EXTRACT FRACTIONS FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES”

2019 ◽  
pp. 266-272
Author(s):  
GARIGE BABA SHANKAR RAO ◽  
SRISAILAM K ◽  
V UMA MAHESHWARA RAO ◽  
VASUDHA B
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Dose Range ◽  
Experimental Studies ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Methanol Fraction ◽  
Cotton Pellet ◽  
Anti Inflammatory

Objective: This current investigation assesses in vivo central and peripheral analgesic effects and anti-inflammatory properties of fractions obtained from Galphimia glauca (GG) stem methanol extract. Methods: The laboratory models such as Swiss albino mice and Wistar albino rats were employed in the studies. The GG stem methanol extract was subjected to fractionation with solvents such as hexane, chloroform, ethyl acetate, and methanol. Orally, the dose range of 100, 200, and 400 mg/kg was given for 1 day for evaluating analgesic (hotplate test, tail clip test, writhing test, and formalin test) and weekdays for assessing anti-inflammatory activity (carrageenan and cotton pellet test methods), respectively. The experimental studies were further conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by prechallenging it with naloxone and acetic acid, respectively. The in vivo anti-inflammatory studies were conducted using carrageenan-induced rat paw edema model and cotton pellet granuloma test. Results: The LD50 of the extract was found to be >2000 mg/kg b.w. The methanol fraction of 400 mg/kg dose exhibited significant (p≤0.001) and dose-dependent analgesic and anti-inflammatory activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid, respectively. Conclusion: The results revealed that the stem methanol fraction has more potential in terms of analgesic and anti-inflammatory properties.

Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract

2018 ◽  
Vol 30 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Olufunmilayo O. Adeyemi ◽  
Ismail O. Ishola ◽  
Elizabeth T. Adesanya ◽  
Destiny O. Alohan
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Potassium Channel ◽  
Leaf Extract ◽  
Paw Edema ◽  
Hot Plate ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Tetracera Alnifolia ◽  
Inflammatory Effect

Abstract Background Tetracera alnifolia Willd. (Dilleniaceae) is used in traditional African Medicine for the treatment of headache, abdominal pain, and rheumatism. Hence, this study sought to investigate the antinociceptive and anti-inflammatory effects of the hydroethanolic leaf extract of T. alnifolia (HeTA) in rodents. Methods Antinociceptive activity was evaluated using the acetic acid-induced writhing, formalin-/capsaicin-induced paw licking and hot plate tests in mice. The contribution of opioidergic, l-arginine-nitric oxide, and ATP-sensitive potassium channel pathways in HeTA-induced antinociception was also evaluated. The anti-inflammatory effect was assessed using the carrageenan-induced paw edema, xylene ear edema, cotton pellet granuloma, and complete Freund’s adjuvant (CFA)-induced arthritis in rats. Results HeTA (100, 200, and 400 mg/kg, p.o.) produced significant (p<0.05) decrease in mean number of acetic acid-induced writhing, time spent licking paw in formalin, and capsaicin tests as well as time course increase in nociceptive reaction latency in hot plate test. HeTA-induced antinociception was prevented by pretreatment of mice with naloxone (non-selective opioid receptor antagonist), l-arginine (nitric oxide precursor), or glibenclamide (ATP-sensitive potassium channel blocker). HeTA (100 mg/kg, p.o.) produced a significant anti-inflammatory effect against carrageenan-induced rat paw edema (1–5 h), xylene-induced ear edema, cotton pellet-induced granuloma formation, and CFA-induced arthritis in rats. The effects of HeTA in various models were similar to the effect of the standard reference drugs. Conclusions Findings from this study showed that HeTA possesses antinociceptive effect possibly mediated through peripheral opioid receptors with activation of l-arginine-nitric oxide and ATP-sensitive potassium channel pathway as well as anti-inflammatory activity.

scholarly journals Analgesic, anti-inflammatory, anticonvulsant and CNS effect of the ethanolic extract of Butea monosperma roots

2016 ◽  
Vol 4 (1) ◽  
pp. 9-18
Author(s):  
Shah Md Anisul Arefin ◽  
Md Alimul Islam ◽  
Syeda Tahmina Binte Rashid ◽  
Md Abu Rayhan ◽  
Md Monir Hossen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethanolic Extract ◽  
Significant Inhibition ◽  
Sedative Effect ◽  
Butea Monosperma ◽  
Sleeping Time ◽  
Cotton Pellet ◽  
Time Test ◽  
Anti Inflammatory ◽  
Anticonvulsant Activities

The analgesic, anti-inflammatory, anticonvulsant and CNS activities of the ethanolic extract of Butea monosperma roots were examined. The extract produced no significant inhibition of abdominal constrictions caused by acetic acid. It showed significant inhibition of the earlyphase of formalin-induced pain, also reduced the weight of granuloma induced by a cotton pellet. Moreover, the extract caused a moderate inhibition of ear swelling in mice caused by croton oil. In pentobarbital induced sleeping time test the extract slightly reduced the onset and increased the duration of sleeping. Therefore, Butea monosperma showed analgesic, antiinflammatory, mild anticonvulsant activities as well as sedative effect on CNS.Jahangirnagar University J. Biol. Sci. 4(1): 9-18, 2015 (June)

scholarly journals Anti-Inflammatory Effect of Supercritical-Carbon Dioxide Fluid Extract from Flowers and Buds ofChrysanthemum indicumLinnén

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Xiao-Li Wu ◽  
Chu-Wen Li ◽  
Hai-Ming Chen ◽  
Zu-Qing Su ◽  
Xiao-Ning Zhao ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Acetic Acid ◽  
Chemical Composition ◽  
Supercritical Carbon Dioxide ◽  
Granuloma Formation ◽  
Ear Edema ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Inflammatory Effect ◽  
Supercritical Carbon

The aim of this study was to analyze the chemical composition and investigate the anti-inflammatory property of the supercritical-carbon dioxide extract from flowers and buds ofC. indicum(CISCFE). The anti-inflammatory effect was evaluated in four animal models including xylene-induced mouse ear edema, acetic acid-induced mouse vascular permeability, carrageenan-induced mouse hind paw edema, and cotton pellet-induced rat granuloma formation. The results indicated that CISCFEsignificantly attenuated xylene-induced ear edema, decreased acetic acid-induced capillary permeability, reduced carrageenan-induced paw, and inhibited the cotton pellet-induced granuloma formation in a dose-dependent manner. Histopathologically, CISCFEabated inflammatory response of the edema paw. Preliminary mechanistic studies demonstrated that CISCFEdecreased the MDA level via increasing the activities of anti-oxidant enzymes (SOD, GPx, and GRd), attenuated the productions of NF-κB, TNF-α, IL-1β, IL-6, PGE2and NO, and suppressed the activities of iNOS and COX-2. In phytochemical study, 35 compounds were identified by GC-MS, and 5 compounds (chlorogenic acid, luteolin-7-glucoside, linarin, luteolin and acacetin) were reconfirmed and quantitatively determined by HPLC-PAD. This paper firstly analyzed the chemical composition by combining GC-MS with HPLC-PAD and explored possible mechanisms for the anti-inflammatory effect of CISCFE.

scholarly journals Anti-inflammatory and Analgesic Activities of Asteracantha longifolia Nees

2012 ◽  
Vol 15 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Md Al Amin ◽  
Ishtiaque A Chowdhury ◽  
KMM Mahbub ◽  
Mafruhi Sattar ◽  
Masum Shahriar ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Significant Inhibition ◽  
Leukocyte Migration ◽  
Croton Oil ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Analgesic Activities

The present study was designed to evaluate the analgesic and anti-inflammatory activities of the ethanol extract of whole plant of Asteracantha longifolia Nees (family Acanthaceae) in mice. The analgesic activity was determined for its central and peripheral pharmacological actions using hotplate, formalin induced pain and acetic acid-induced writhing test in mice. Anti inflammatory effects were determined by ear swelling induced by croton oil, xyleneinduced ear edema, leukocyte migration induced by carrageenan, cotton pellet-induced granuloma formation. Tramadol (10 mg/kg) and Ibuprofen (100 mg/kg) were used as reference analgesic agents. The crude ethanol extract showed a significant inhibition of ear swelling caused by croton oil and xylene in mice. The crude extract decreased leukocyte migration induced by carrageenan, also moderately inhibited the weight of granuloma induced by a cotton pellet, as well as the formalin-induced pain. The extract given by p.o. route, produced significant inhibition of abdominal constrictions caused by acetic acid. Moreover, the extract also showed moderate analgesic activity on the hot plate pain threshold in mice. These data demonstrated that the plant may contain bioactive compounds possessing anti-inflammatory and analgesic activities. DOI: http://dx.doi.org/10.3329/bpj.v15i2.12586 Bangladesh Pharmaceutical Journal 15(2): 171-176, 2012

scholarly journals Studies on Anti-inflammatory, Antinociceptive and Antipyretic Activities of Ethanol Extract of Azadirachta indica Leaves

1970 ◽  
Vol 44 (2) ◽  
pp. 199-206 ◽  
Author(s):  
M Mahabub-Uz-Zaman ◽  
Nazim Uddin Ahmed ◽  
Rasheda Akter ◽  
Kabir Ahmed ◽  
M Shafiqul Islam Aziz ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dose Level ◽  
Azadirachta Indica ◽  
Ethanol Extract ◽  
High Dose ◽  
Paw Edema ◽  
High Dose Level ◽  
Antipyretic Effect ◽  
Cotton Pellet ◽  
Anti Inflammatory

The present study was carried out to investigate the anti-inflammatory, antinociceptive and antipyretic activities of the crude ethanol extract of Azadirachta indica leaves on experimental animal model at three different dose level- 1g/kg, 500 mg/kg and 100 mg/kg. For evaluation of the anti-inflammatory property carrageenan induced paw edema served as acute model and cotton pellet induced granuloma formation served as chronic model in rats. The acetic acid induced writhing response and hot plate test were used to assess antinociceptive activity in mice. The antipyretic activity was evaluated on yeast-induced pyrexia in rats. Acute toxicity test was also performed in rats after administration of the extract orally at high dose level (4g/kg). Oral administration of the ethanol extract of A. indica leaves significantly (P<0.05) suppressed the paw edema induced by carrageenan as well as granulomatous tissue formation induced by cotton pellet in rats at high dose level (1g/kg) but no significant effect was observed at 500 mg/kg and 250mg/kg dose level. In addition, ethanol extract obtained from A. indica leaves (1g/ kg) also significantly (P<0.05) attenuated the acetic acid induced writhing response in mice but did not show any significant protective effect on heat induced pain in mice. A significant antipyretic effect (P< 0.05) was noticed with ethanol leaf extract of A. indica leaves at 1g/kg and 500 mg/kg dose level. In acute toxicity study, no mortality was observed at 4 g/kg dose level. The present study also demonstrates the potential anti-inflammatory, analgesic and antipyretic effect of the ethanol extract of Azadirachta indica leaves, which supports the claims by the traditional medicine practitioners. Keywords: Anti-inflammatory, Antinociceptive, Antipyretic, Azadirachta indica DOI: 10.3329/bjsir.v44i2.3672 Bangladesh J. Sci. Ind. Res. 44(2), 199-206, 2009

scholarly journals Analgesic and Anti-Inflammatory Activities of Leaf Extract ofMallotus repandus(Willd.) Muell. Arg.

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Mahadi Hasan ◽  
Nizam Uddin ◽  
Md. Rakib Hasan ◽  
A. F. M. Mahmudul Islam ◽  
Md. Monir Hossain ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Traditional Use ◽  
Rheumatic Arthritis ◽  
Ear Edema ◽  
Cotton Pellet ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Mallotus Repandus

In folk medicineMallotus repandus(Willd.) Muell. Arg. is used to treat muscle pain, itching, fever, rheumatic arthritis, snake bite, hepatitis, and liver cirrhosis. This study aimed to evaluate the antinociceptive as well as the anti-inflammatory activities of the methanol extract of leaf. The leaves were extracted with methanol following hot extraction and tested for the presence of phytochemical constituents. Analgesic and anti-inflammatory activities were evaluated using acetic acid induced writhing test, xylene induced ear edema, cotton pellet induced granuloma, and tail immersion methods at doses of 500, 1000, and 2000 mg/kg body weight. The presence of flavonoids, saponins, and tannins was identified in the extract. The extract exhibited considerable antinociceptive and anti-inflammatory activities against four classical models of pain. In acetic acid induced writhing, xylene induced ear edema, and cotton pellet granuloma models, the extract revealed dose dependent activity. Additionally, it increased latency time in tail immersion model. It can be concluded thatM. repanduspossesses significant antinociceptive potential. These findings suggest that this plant can be used as a potential source of new antinociceptive and anti-inflammatory candidates. The activity of methanol extract is most likely mediated through central and peripheral inhibitory mechanisms. This study justified the traditional use of leaf part of this plant.

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