scholarly journals Analgesic and Anti-Inflammatory Activities of Leaf Extract ofMallotus repandus(Willd.) Muell. Arg.

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Mahadi Hasan ◽  
Nizam Uddin ◽  
Md. Rakib Hasan ◽  
A. F. M. Mahmudul Islam ◽  
Md. Monir Hossain ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Traditional Use ◽  
Rheumatic Arthritis ◽  
Ear Edema ◽  
Cotton Pellet ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Mallotus Repandus

In folk medicineMallotus repandus(Willd.) Muell. Arg. is used to treat muscle pain, itching, fever, rheumatic arthritis, snake bite, hepatitis, and liver cirrhosis. This study aimed to evaluate the antinociceptive as well as the anti-inflammatory activities of the methanol extract of leaf. The leaves were extracted with methanol following hot extraction and tested for the presence of phytochemical constituents. Analgesic and anti-inflammatory activities were evaluated using acetic acid induced writhing test, xylene induced ear edema, cotton pellet induced granuloma, and tail immersion methods at doses of 500, 1000, and 2000 mg/kg body weight. The presence of flavonoids, saponins, and tannins was identified in the extract. The extract exhibited considerable antinociceptive and anti-inflammatory activities against four classical models of pain. In acetic acid induced writhing, xylene induced ear edema, and cotton pellet granuloma models, the extract revealed dose dependent activity. Additionally, it increased latency time in tail immersion model. It can be concluded thatM. repanduspossesses significant antinociceptive potential. These findings suggest that this plant can be used as a potential source of new antinociceptive and anti-inflammatory candidates. The activity of methanol extract is most likely mediated through central and peripheral inhibitory mechanisms. This study justified the traditional use of leaf part of this plant.

scholarly journals Analgesic and Anti-Inflammatory Activities of Diethyl Ether and n-Hexane Extract of Polyalthia suberosa Leaves

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Nelufar Yasmen ◽  
Md. Abdullah Aziz ◽  
Afsana Tajmim ◽  
Mst. Irin Akter ◽  
Amit Kumar Hazra ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Diethyl Ether ◽  
Folk Medicine ◽  
Immersion Test ◽  
Significant Inhibition ◽  
Writhing Test ◽  
Ear Edema ◽  
Tail Immersion ◽  
Hexane Extracts ◽  
Anti Inflammatory

In folk medicine, Polyalthia suberosa is used as abortifacient, laxative, febrifuge analgesic, filler of tooth cavities, and anti-HIV drug and for rheumatism and various skin infections. The present study was directed to evaluate the analgesic and anti-inflammatory activities of diethyl ether and n-hexane extracts of Polyalthia suberosa leaves (PSDE and PSNH). A variety of tests including formalin-induced paw licking test, acetic acid induced writhing test, and tail immersion test were used to assess the analgesic activity. In addition, xylene-induced ear edema test was used to evaluate anti-inflammatory activity of PSDE and PSNH. PSDE and PSNH at 200 and 400 mg/kg doses expressed analgesic as well as anti-inflammatory activities in mice. In formalin-induced paw licking test, acetic acid induced writhing test, and xylene-induced ear edema test, the extracts exhibited significant inhibition (P⁎<0.05 versus control) of pain and inflammation. Alternatively, in tail immersion test, PSDE 400 mg/kg showed significant (P⁎<0.05 versus control) latency at 30 min but another tested sample had no significant latency. From this study, it could be shown that Polyalthia suberosa leaves may contain analgesic and anti-inflammatory agents which support its use in traditional medicine.

Effect of Nectaroscordum koelzi Methanolic Extract on Acute and Chronic Inflammation in Male Mice

2020 ◽  
Vol 20 ◽  
Author(s):  
Karam Hossein Hasanvand ◽  
Mojtaba Khaksarian ◽  
Maryam Alipour ◽  
Hormoz Mahmoudvand ◽  
Massumeh Naizi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Methanol Extract ◽  
Significant Activity ◽  
Paw Edema ◽  
Ear Edema ◽  
Edema Volume ◽  
Cotton Pellet ◽  
Acute And Chronic Inflammation

Introduction: The present study deals with the effect of Nectaroscordum koelzi fruit extract on acute and chronic inflammation. Methods: Totally, 84 NMRI mice were used in this study. The extract effect on acute inflammation was analyzed by increasing vascular permeability via acetic acid and xylene induced ear edema among mice. The extract was evaluated in terms of effects on chronic inflammation by means of the cotton pellet test among mice. For the assessment of inflammation degree, the mice paw edema volume was measured by the plethysmometric test. Results: The findings showed that the extract was effective on acute inflammation induced by acetic acid in mice. In the xylene ear edema, N. koelzi extract indicated the significant activity in mice. In the cotton pellet method, the methanol extract produced a significant reduction in comparison with the control and dexamethasone. Mice paw edema volume decreased with the extract. Conclusion: In general, the data from the experiments indicated that the methanol extract of N. koelzi has an anti-inflammatory effect on acute and chronic inflammation. However, the exact contributing mechanisms have not been investigated for the pharmacological effects.

scholarly journals “EVALUATION OF GALPHIMIA GLAUCA STEM METHANOL EXTRACT FRACTIONS FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES”

2019 ◽  
pp. 266-272
Author(s):  
GARIGE BABA SHANKAR RAO ◽  
SRISAILAM K ◽  
V UMA MAHESHWARA RAO ◽  
VASUDHA B
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Dose Range ◽  
Experimental Studies ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Methanol Fraction ◽  
Cotton Pellet ◽  
Anti Inflammatory

Objective: This current investigation assesses in vivo central and peripheral analgesic effects and anti-inflammatory properties of fractions obtained from Galphimia glauca (GG) stem methanol extract. Methods: The laboratory models such as Swiss albino mice and Wistar albino rats were employed in the studies. The GG stem methanol extract was subjected to fractionation with solvents such as hexane, chloroform, ethyl acetate, and methanol. Orally, the dose range of 100, 200, and 400 mg/kg was given for 1 day for evaluating analgesic (hotplate test, tail clip test, writhing test, and formalin test) and weekdays for assessing anti-inflammatory activity (carrageenan and cotton pellet test methods), respectively. The experimental studies were further conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by prechallenging it with naloxone and acetic acid, respectively. The in vivo anti-inflammatory studies were conducted using carrageenan-induced rat paw edema model and cotton pellet granuloma test. Results: The LD50 of the extract was found to be >2000 mg/kg b.w. The methanol fraction of 400 mg/kg dose exhibited significant (p≤0.001) and dose-dependent analgesic and anti-inflammatory activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid, respectively. Conclusion: The results revealed that the stem methanol fraction has more potential in terms of analgesic and anti-inflammatory properties.

scholarly journals Anti-Inflammatory Effect of Supercritical-Carbon Dioxide Fluid Extract from Flowers and Buds ofChrysanthemum indicumLinnén

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Xiao-Li Wu ◽  
Chu-Wen Li ◽  
Hai-Ming Chen ◽  
Zu-Qing Su ◽  
Xiao-Ning Zhao ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Acetic Acid ◽  
Chemical Composition ◽  
Supercritical Carbon Dioxide ◽  
Granuloma Formation ◽  
Ear Edema ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Inflammatory Effect ◽  
Supercritical Carbon

The aim of this study was to analyze the chemical composition and investigate the anti-inflammatory property of the supercritical-carbon dioxide extract from flowers and buds ofC. indicum(CISCFE). The anti-inflammatory effect was evaluated in four animal models including xylene-induced mouse ear edema, acetic acid-induced mouse vascular permeability, carrageenan-induced mouse hind paw edema, and cotton pellet-induced rat granuloma formation. The results indicated that CISCFEsignificantly attenuated xylene-induced ear edema, decreased acetic acid-induced capillary permeability, reduced carrageenan-induced paw, and inhibited the cotton pellet-induced granuloma formation in a dose-dependent manner. Histopathologically, CISCFEabated inflammatory response of the edema paw. Preliminary mechanistic studies demonstrated that CISCFEdecreased the MDA level via increasing the activities of anti-oxidant enzymes (SOD, GPx, and GRd), attenuated the productions of NF-κB, TNF-α, IL-1β, IL-6, PGE2and NO, and suppressed the activities of iNOS and COX-2. In phytochemical study, 35 compounds were identified by GC-MS, and 5 compounds (chlorogenic acid, luteolin-7-glucoside, linarin, luteolin and acacetin) were reconfirmed and quantitatively determined by HPLC-PAD. This paper firstly analyzed the chemical composition by combining GC-MS with HPLC-PAD and explored possible mechanisms for the anti-inflammatory effect of CISCFE.

scholarly journals Antitumor, Analgesic and Anti-inflammatory Activities of Glochidion multiloculare (Rottler ex Willd) Voigt

2015 ◽  
Vol 18 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Selina Kabir ◽  
Ronok Zahan ◽  
Abdullah Mohammad Sarwaruddin Chowdhury ◽  
Mohammad Rashedul Haque ◽  
Mohammad Abdur Rashid
Keyword(s):  
Acetic Acid ◽  
Pain Threshold ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Control Group ◽  
Immersion Method ◽  
Tumor Bearing ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Soluble Fractions

The antitumor, analgesic and anti-inflammatory activities of the methanol extract (MEGM) of Glochidion multiloculare and its petroleum ether (PEFGM), carbon tetrachloride (CTFGM) and chloroform (CHFGM) soluble fractions were investigated. The antitumor activity of the extract/fractions was determined against Ehrlich’s ascites carcinoma (EAC) in mice at 20 mg/kg body weight intraperitoneally. Increase of survival time by 24 ± 0.12, 26 ± 0.40, 21 ± 0.12 and 27 ± 0.42 days was observed by the MEGM, PEFGM, CTFGM and CHFGM treated tumor bearing mice compared to the control group (20 ± 0.12 days). Hematological studies revealed that heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to normal levels was observed in extract/fractions treated animals. Significant decrease in RBC and increase in WBC counts were observed in extract/fractions treated animals when compared to EAC affected animals. Both extract and fractions at the dose of 100 mg/kg b.w. significantly increased the pain threshold in tail immersion method and reduced the writhing caused by acetic acid induced method. The MEGM, PEFGM, CTFGM and CHFGM showed anti-inflammatory activities at 100 mg/kg b.w. This is the first report of antitumor, analgesic and anti-inflammatory potential of G. multiloculare.Bangladesh Pharmaceutical Journal 18(2): 142-148, 2015

scholarly journals TAK1/AP-1-Targeted Anti-Inflammatory Effects of Barringtonia augusta Methanol Extract

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3053
Author(s):  
Anh Thu Ha ◽  
Mi-Yeon Kim ◽  
Jae Youl Cho
Keyword(s):  
Mouse Model ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Activator Protein 1 ◽  
Western Blot Assay ◽  
In Vivo Experiments ◽  
Chemokine Ligand ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

Investigation of Analgesic, Anti-Inflammatory, Hypoglycaemic, Neuropharmacological and Cytotoxic Properties of Clerodendrum viscosum (Leaves)

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Tanny SZ ◽  
◽  
Ropuk RS ◽  
Patowary AA ◽  
Lata L ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Glucose Tolerance ◽  
Brine Shrimp ◽  
Depressant Effect ◽  
Sleeping Time ◽  
Ear Edema ◽  
Cns Depressant ◽  
Croton Oil ◽  
Anti Inflammatory ◽  
Clerodendrum Viscosum

Clerodendrum viscosum (CV) has been used traditionally to treat medical problems like asthma, ulcer, inflammation, pyrexia, diabetes, malaria, skin diseases, snakebite and tumor by folk practitioners. The present study evaluated the analgesic, antiinflammatory, neuropharmacological and cytotoxic properties C. viscosum (leaves) in rodents. Swiss albino mice of either sex weighing 25-30 gm and SD rats (150-180 mg) were divided into control (DW), standard (model specific) as well as test groups (n=6). Analgesic potential was evaluated using acetic acid-induced writhing and formalin induced pawlicking test. Anti-inflammatory properties were evaluated by xylene and croton oil induced ear edema test. Glucose tolerance was evaluated by OGTT in normal rats. Pentobarbital induced sleeping time test was applied to assess neuropharmacological activity. Also, Brine shrimp lethality bioassay method was employed for cytotoxicity evaluation. The alcoholic extracts showed significant antinociceptive activity in acetic acid test (p<0.01) and formalin test (p<0.05) at the dose of 1000mg/kg bw. The crude extract reduced inflammation significantly (p<0.01) in both xylene and croton oil induced ear edema test. At the dose of 1000mg/kg it increased glucose tolerance significantly (p<0.05) in normal rats. CV extract significantly (p<0.01) increased sleeping time indicating CNS depressant effect. The extract exhibited a potent cytotoxicity against brine shrimp (LC50=316.23μg/ml). C. viscosum leaves showed analgesic, antiinflammatory, hypoglycemic and CNS depressant effect against experimentally induced model mice. It also possessed cytotoxic properties and further studies are required to evaluate these effects and the potential of the plant.

scholarly journals Bioactivities of the ethanol extract from Ageratum fastigiatum branches: antioxidant, antinociceptive and anti-inflammatory

2016 ◽  
Vol 88 (3) ◽  
pp. 1471-1484
Author(s):  
GLAUCIEMAR DEL-VECHIO-VIEIRA ◽  
BRUNA C.S. SANTOS ◽  
MARIA SILVANA ALVES ◽  
AÍLSON L.A. ARAÚJO ◽  
CÉLIA H. YAMAMOTO ◽  
...  
Keyword(s):  
Antinociceptive Effect ◽  
Folk Medicine ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Immersion Test ◽  
Significant Inhibition ◽  
Paw Edema ◽  
Hot Plate ◽  
Tail Immersion ◽  
Anti Inflammatory

ABSTRACT The present study was designed to investigate the antioxidant, antinociceptive and anti-inflammatory activities of the ethanol extract from Ageratum fastigiatum branches. Phytochemical screening and total phenol and flavonoid contents were determined. The antioxidant activity was assessed by 2,2-diphenyl-1-pycrilhydrazin (DPPH) and iron reducing power methods. The antinociceptive effect was evaluated using the acetic acid-induced writhing, formalin, hot plate and tail immersion assays; while the carrageenan-induced paw edema and pleurisy tests were performed to examine the anti-inflammatory activity against acute inflammation. The extract revealed the presence of flavonoids, tannins, coumarins, terpenes, sterols and saponins. Expressive levels of total phenols and flavonoids and a promising antioxidant effect were quantified. At the doses of 50, 100 and 200 mg/kg, the extract inhibited the writhing, reduced both phases of paw licking time and increased the reaction time on the hot plate. In the tail immersion test, the extract (50, 100 and 200 mg/kg) caused a significant inhibition of pain. In these doses, the paw edema, exudate volume and leucocyte mobilization were significantly reduced. These results suggest that A. fastigiatum can be an active source of substances with antioxidant, antinociceptive and anti-inflammatory activities, adding scientific support to the appropriate use in the Brazilian folk medicine.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

scholarly journals Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Cuauhtemoc Pérez González ◽  
Roberto Serrano Vega ◽  
Marco González-Chávez ◽  
Miguel Angel Zavala Sánchez ◽  
Salud Pérez Gutiérrez
Keyword(s):  
Medicinal Plant ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Active Fraction ◽  
Ear Edema ◽  
Methanol Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

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