FABRICATION AND CHARACTERIZATION OF CALCIUM SILICATE SCAFFOLDS FOR TISSUE ENGINEERING

2014 ◽  
Vol 14 (04) ◽  
pp. 1450049 ◽  
Author(s):  
CIJUN SHUAI ◽  
ZHONGZHENG MAO ◽  
ZIKAI HAN ◽  
SHUPING PENG ◽  
ZHENG LI
Keyword(s):  
Tissue Engineering ◽  
Calcium Silicate ◽  
Laser Power ◽  
Selective Laser Sintering ◽  
Biological Properties ◽  
High Temperature Phase ◽  
Temperature Phase ◽  
Conventional Technology

Calcium silicate ( CaSiO 3) is a promising material due to its favorable biological properties. However, it was difficult to fabricate ceramic scaffolds with interconnected porous structure via conventional technology. In present study, CaSiO 3 scaffolds with totally interconnected pores were fabricated via selective laser sintering (SLS). The microstructure, mechanical and biological properties were examined. The results revealed that the powder gradually fused together with the reduction of voids and the elimination of particle boundary as the laser power increased in the range of 3–15 W with scanning electron microscope. Meanwhile the low-temperature phase (β- CaSiO 3) transformed into high-temperature phase (α- CaSiO 3) gradually, which decreased the mechanical properties of the obtained scaffolds. Besides, the compressive strength increased from 12.9 ± 2.34 MPa to 18.19 ± 1.24 MPa (the laser power is 12 w) and then decreased gradually with increasing laser power. In vitro biological properties of CaSiO 3 scaffolds sintered under optimal conditions indicated that the distribution of apatite mineralization became uniform as the amount of them increased after being immersed in simulated body fluids. In the meantime, the thin cytoplasmic extensions of MG-63 cells increased until formed a dense cell layer after 1–5 days of cell culture. The results suggested that the CaSiO 3 scaffold fabricated via SLS has potential application for bone tissue engineering.

scholarly journals Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 85 ◽  
Author(s):  
Truc Cong Ho ◽  
Jin-Seok Park ◽  
Sung-Yeoul Kim ◽  
Hoyeol Lee ◽  
Ju-Sop Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Subcritical Water ◽  
Biological Properties ◽  
Ethanol Solution ◽  
Water Soluble ◽  
Potential Candidate ◽  
High Porosity ◽  
Engineering Applications ◽  
Molecular Weights

For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the distinctive properties of low MW collagen-based scaffolds with alginate unmodified and modified by subcritical water. Besides, we developed a facile method to cross-link water-soluble scaffolds using glutaraldehyde in an aqueous ethanol solution. The prepared cross-linked scaffolds showed good structural properties with high porosity (~93%) and high cross-linking degree (50–60%). Compared with collagen (6000 Da)-based scaffolds, collagen (25,000 Da)-based scaffolds exhibited higher stability against collagenase degradation and lower weight loss in phosphate buffer pH 7.4. Collagen (25,000 Da)-based scaffolds with modified alginate tended to improve antioxidant capacity compared with scaffolds containing unmodified alginate. Interestingly, in vitro coagulant activity assay demonstrated that collagen (25,000 Da)-based scaffolds with modified alginate (C25-A63 and C25-A21) significantly reduced the clotting time of human plasma compared with scaffolds consisting of unmodified alginate. Although some further investigations need to be done, collagen (25,000 Da)-based scaffolds with modified alginate should be considered as a potential candidate for tissue engineering applications.

scholarly journals In Vitro Characterization of Immune-Related Properties of Human Fetal Bone Cells for Potential Tissue Engineering Applications

2009 ◽  
Vol 15 (7) ◽  
pp. 1523-1532 ◽  
Author(s):  
Marc-Olivier Montjovent ◽  
Chiara Bocelli-Tyndall ◽  
Corinne Scaletta ◽  
Arnaud Scherberich ◽  
Silke Mark ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Cells ◽  
Engineering Applications ◽  
Fetal Bone ◽  
In Vitro Characterization

Fabrication and Characterization of in vitro 2D Skin Model - An attempt to establish Scaffold for Tissue Engineering

2021 ◽  
Author(s):  
Rajesh Pandiyan ◽  
Abimanyu Sugumaran ◽  
Sumathi Samiappan ◽  
Parameshwaran Sengottaiyan ◽  
Sivasankaran Ayyaru ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Model ◽  
Fabrication And Characterization

scholarly journals Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering

2021 ◽  
Vol 22 (21) ◽  
pp. 11600
Author(s):  
Dong Jin Choi ◽  
Kyoung Choi ◽  
Sang Jun Park ◽  
Young-Jin Kim ◽  
Seok Chung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Physical Properties ◽  
Dimensional Stability ◽  
3D Structure ◽  
Biological Properties ◽  
Dermal Fibroblasts ◽  
3D Scaffolds ◽  
3D Printed

Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks.

scholarly journals In vitro characterization of a novel magnetic fibrin-agarose hydrogel for cartilage tissue engineering

2020 ◽  
Vol 104 ◽  
pp. 103619 ◽  
Author(s):  
Ana Belén Bonhome-Espinosa ◽  
Fernando Campos ◽  
Daniel Durand-Herrera ◽  
José Darío Sánchez-López ◽  
Sébastien Schaub ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Vitro Characterization ◽  
Agarose Hydrogel

scholarly journals Fabrication and Characterization of Scaffolds of Poly(ε-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel Aparecido Lopes Vieira da Cunha ◽  
Paulo Inforçatti Neto ◽  
Kelli Cristina Micocci ◽  
Caroline Faria Bellani ◽  
Heloisa Sobreiro Selistre-de-Araujo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Manufacturing Process ◽  
Potential Application ◽  
Morphological Characterization ◽  
Mechanical Compression ◽  
Compression Tests ◽  
Extrusion Head ◽  
Fabrication And Characterization

Scaffolds of poly(ε-caprolactone) (PCL) and their biocomposites with 0, 1, 3, and 5 wt.% Biosilicate® were fabricated by the generative manufacturing process coupled with a vertical miniscrew extrusion head to application for restoration of bone tissue. Their morphological characterization indicated the designed 0°/90° architecture range of pore sizes and their interconnectivity is feasible for tissue engineering applications. Mechanical compression tests revealed an up to 57% increase in the stiffness of the scaffold structures with the addition of 1 to 5 wt.% Biosilicate® to the biocomposite. No toxicity was detected in the scaffolds tested by in vitro cell viability with MC3T3-E1 preosteoblast cell line. The results highlighted the potential application of scaffolds fabricated with poly(ε-caprolactone)/Biosilicate® to tissue engineering.

Modification of Thai Silk Fibroin Scaffolds by Gelatin Conjugation for Tissue Engineering

2008 ◽  
Vol 55-57 ◽  
pp. 685-688 ◽  
Author(s):  
J. Chamchongkaset ◽  
Sorada Kanokpanont ◽  
David L. Kaplan ◽  
Siriporn Damrongsakkul
Keyword(s):  
Tissue Engineering ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Three Dimensional ◽  
Biological Properties ◽  
Salt Leaching ◽  
Biological Compatibility ◽  
Salt Crystals

Silk has been used commercially as biomedical sutures for decades. Recently silk fibroin, especially from Bombyx mori silkworm, has been explored for many tissue engineering applications such as bone and cartilage due to its impressive biological compatibility and mechanical properties. In Thailand, Thai native silkworms have been long cultivated. Distinct characteristics of cocoon Thai silk are its yellow color and coarse filament. There is more sericin in Thai silk than in other Bombyx mori silks. These characteristics provide Thai silk a unique texture for textile industry. It is therefore the aim of this study to develop three-dimensional silk fibroin-based scaffolds from Thai yellow cocoon “Nangnoi-Srisaket” of Bombyx mori silkworms using salt-leaching method. To enhance the biological properties, type A gelatin, the denature form of collagen having good biocompactibility, was used to conjugate with silk fibroin scaffolds. The pore size of salt-leached silk fibroin scaffold structure represented the size of salt crystals used (600-710µm). After gelatin conjugation, gelatin was partly formed fibers inside the pores of silk fibroin scaffolds resulting in fiber-like structure with highly interconnection. Gelatin conjugation enhanced the compressive modulus of silk fibroin scaffolds by 93%. The results on in vitro culture using mouse osteoblast-like cells (MC3T3-E1) showed that gelatin conjugation could promote the cell proliferation in silk fibroin scaffolds. Moreover, the observed morphology of cells proliferated inside the scaffold after 14 days of culture showed the larger spreading area of cells on conjugated gelatin/silk fibroin scaffolds, compared to round-shaped cells on silk fibroin scaffolds. The results implied that Thai silk fibroin looked promising to be applied in tissue engineering and gelatin conjugation on Thai silk fibroin scaffolds could enhance the biological properties of scaffolds.

Facile synthesis and characterization of high temperature phase FeS2 pyrite nanocrystals

2012 ◽  
Vol 75 ◽  
pp. 152-154 ◽  
Author(s):  
Su-Ching Hsiao ◽  
Chih-Ming Hsu ◽  
Szu-Ying Chen ◽  
Yu-Hsun Perng ◽  
Yu-Lun Chueh ◽  
...  
Keyword(s):  
High Temperature ◽  
High Temperature Phase ◽  
Synthesis And Characterization ◽  
Temperature Phase ◽  
Facile Synthesis ◽  
Fes2 Pyrite

Preparation and Characterization of a Novel γ-PGA/β-Tricalcium Phosphate Composite for Tissue Engineering

2014 ◽  
Vol 900 ◽  
pp. 306-311 ◽  
Author(s):  
Xiu Lin Shu ◽  
Qing Shan Shi ◽  
Xiao Bao Xie ◽  
Xiao Mo Huang ◽  
Yi Ben Chen
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Porous Structure ◽  
Water Absorption ◽  
Tricalcium Phosphate ◽  
Absorption Rate ◽  
Swelling Ratio ◽  
Composite Scaffolds

In order to improvedβ-TCP biocompatibility and cell growth, was chosen to modify β-TCP matrices to produce a γ-PGA/β-TCP composite biomaterial. Then, the morphology, water uptake and retention abilities,in vitrodegradation property in the simulated medium, cytotoxicity of this novel γ-PGA/β-TCP composite is investigated. SEM shows that the γ-PGA/β-TCP composite has a porous structure. By increasing the percentage ofγ-PGA from 0% to 50%, the swelling ratio of the composite s was enhanced from 9.0%to 297%. These data suggested that the surface hydrophilicity, water absorption rate, and swelling ratio were improved by adding γ-PGA to the composite. In the cytocompatibility test, the density of MC3T3-E1 preosteoblasts cells on the PTCP1:1 leachates was almost 110% higher than that on the controls on day 3. Therefore, the γ-PGA/β-TCP composite scaffolds, due to their better hydrophilicity, cytocompatibility, and porous structure, are very promising biomaterials for tissure engineering applications.

scholarly journals Synthesis and Properties of Flexible Polyurethane Using Ferric Catalyst for Hypopharyngeal Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Zhisen Shen ◽  
Jian Wang ◽  
Dakai Lu ◽  
Qun Li ◽  
Chongchang Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Matrix Material ◽  
Biological Properties ◽  
Hexamethylene Diisocyanate ◽  
Engineering Research ◽  
Biodegradable Polyurethane ◽  
Flexible Polyurethane ◽  
Hydrophilic Polyurethane

Biodegradable polyurethane is an ideal candidate material to fabricate tissue engineered hypopharynx from its good mechanical properties and biodegradability. We thus synthesized a hydrophilic polyurethane via reactions among polyethylene glycol (PEG), e-caprolactone (e-CL) and hexamethylene diisocyanate (HDI), and thrihydroxymethyl propane (TMP). The product possessed a fast degradability due to its good wettability and good mechanical parameters with the elongations at break (137 ± 10%) and tensile strength (4.73 ± 0.46 MPa), which will make it a good matrix material for soft tissue like hypopharynx. Its biological properties were evaluated viain vitroandin vivotests. The results showed that this hydrophilic polyurethane material can support hypopharyngeal fibroblast growth and owned good degradability and low inflammatory reaction in subcutaneous implantation. It will be proposed as the scaffold for hypopharyngeal tissue engineering research in our future study.

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