THE WHITE ROMAN GOOSE AS A HOST FOR INFECTION AND VIRAL SHEDDING OF MUSCOVY DUCK PARVOVIRUS

Waterfowl parvoviruses are divided into two groups: the goose parvovirus (GPV) group and the Muscovy duck parvovirus (MDPV) group. Previous study shows that GPV causes the disease in both geese and Muscovy ducks whereas MDPV causes the disease only in ducks but not in geese. However, the possibility remains that MDPV might cause asymptomatic infection in geese. In this study, the white Roman geese were experimentally inoculated with MDPV. Polymerase chain reaction (PCR) analysis showed that the geese inoculated with MDPV shed virus from cloaca from one to four weeks post-inoculation. Western blot analysis showed that these geese also produced antibodies against MDPV from three weeks post-inoculation. In addition, the presence of MDPV in field samples collected from geese was confirmed by PCR and sequencing analysis. Taken together, these results indicated that the goose is a host for infection and viral shedding of MDPV. This finding is important for the control of MDPV infection in the field.

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Molecular Characterization and Pathogenicity of the Novel Recombinant Muscovy Duck Parvovirus Isolated from Geese

Animals ◽

10.3390/ani11113211 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3211

Author(s):

Kuang-Po Li ◽

Yu-Chen Hsu ◽

Chih-An Lin ◽

Poa-Chun Chang ◽

Jui-Hung Shien ◽

...

Keyword(s):

Genomic Sequence ◽

Economic Losses ◽

High Morbidity ◽

Post Inoculation ◽

Muscovy Duck ◽

The Novel ◽

Highly Pathogenic ◽

Goose Parvovirus ◽

Challenge Tests ◽

Muscovy Duck Parvovirus

Goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV) are the main agents associated with waterfowl parvovirus infections that caused great economic losses in the waterfowl industry. In 2020, a recombinant waterfowl parvovirus, 20-0910G, was isolated in a goose flock in Taiwan that experienced high morbidity and mortality. The whole genome of 20-0910G was sequenced to investigate the genomic characteristics of this isolate. Recombination analysis revealed that, like Chinese rMDPVs, 20-0910G had a classical MDPV genomic backbone and underwent two recombination events with classical GPVs at the P9 promoter and partial VP3 gene regions. Phylogenetic analysis of the genomic sequence found that this goose-origin parvovirus was highly similar to the circulating recombinant MDPVs (rMDPVs) isolated from duck flocks in China. The results of experimental challenge tests showed that 20-0910G caused 100% mortality in goose embryos and in 1-day-old goslings by 11 and 12 days post-inoculation, respectively. Taken together, the results indicated that this goose-origin rMDPV was closely related to the duck-origin rMDPVs and was highly pathogenic to young geese.

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Immunologic cross-reactivity between Muscovy duck parvovirus and goose parvovirus on the basis of epitope prediction

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822013000200031 ◽

2013 ◽

Vol 44 (2) ◽

pp. 519-521 ◽

Cited By ~ 6

Author(s):

Ming Li ◽

Tian-fei Yu

Keyword(s):

Epitope Prediction ◽

Cross Reactivity ◽

Muscovy Duck ◽

Goose Parvovirus ◽

Muscovy Duck Parvovirus

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Identification of recombination between Muscovy duck parvovirus and goose parvovirus structural protein genes

Archives of Virology ◽

10.1007/s00705-015-2541-9 ◽

2015 ◽

Vol 160 (10) ◽

pp. 2617-2621 ◽

Cited By ~ 13

Author(s):

Hongxing Shen ◽

Wen Zhang ◽

Hua Wang ◽

Yang Zhou ◽

Shihe Shao

Keyword(s):

Muscovy Duck ◽

Structural Protein ◽

Goose Parvovirus ◽

Muscovy Duck Parvovirus

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STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

Jurnal Riset Akuakultur ◽

10.15578/jra.13.1.2018.85-92 ◽

2018 ◽

Vol 13 (1) ◽

pp. 85 ◽

Cited By ~ 9

Author(s):

Isti Koesharyani ◽

Lila Gardenia ◽

Zakiyah Widowati ◽

Khumaira Khumaira ◽

Dita Rustianti

Keyword(s):

Polymerase Chain Reaction ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Rna Virus ◽

Pcr Analysis ◽

Sequencing Analysis ◽

Accession Number ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain

Ikan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1).Oreochromis niloticus is the main consumption fish commodity in Indonesia. The mortality cases of Nile tilapia have occurred in several culture sites in Java, Lombok, and Sumatra due to the infection of Orthomyxovirus, Tilapia Lake Virus (TiLV). The purpose of this study was to detect the presence of TiLV infection in mass mortality case of Nile tilapia culture using the semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Fish samples were sourced from Segerongan Village Lingsar District, Lombok, West Nusa Tenggara. For RT-PCR analysis, samples from fish brain, kidney, spleen, and liver were collected and treated for sequencing analysis. The visual observation on the moribund tilapia had found several specific clinical symptoms such as eye cataract with sunken eyes, skin abrasion, and darkened body coloration. The result of RT-PCR analysis showed that mass mortalities of tilapia fish had been suspective caused by the infection TiLV RNA virus. The sequencing analysis showed that TiLV samples from Lombok have a genetic similarity of 97% with TiLV from Israeli (Genebank Accession Number KU 751816.1).

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Transformation of Amorphadiene Synthase and Antisilencing P19 Genes into Artemisia annua L. and its Effect on Antimalarial Artemisinin Production

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2020.057 ◽

2020 ◽

Vol 10 (3) ◽

pp. 464-471

Author(s):

Elfahmi Elfahmi ◽

Fany Mutia Cahyani ◽

Tati Kristianti ◽

Sony Suhandono

Keyword(s):

Hairy Root ◽

Hairy Roots ◽

Artemisia Annua ◽

Pcr Analysis ◽

Sequencing Analysis ◽

Pcr Products ◽

Transformed Hairy Root ◽

Polymerase Chain ◽

Infiltration Method

Purpose : The low content of artemisinin related to the biosynthetic pathway is influenced by the role of certain enzymes in the formation of artemisinin. The regulation of genes involved in artemisinin biosynthesis through genetic engineering is a choice to enhance the content. This research aims to transform ads and p19 gene as an antisilencing into Artemisia annua and to see their effects on artemisinin production. Methods: The presence of p19 and ads genes was confirmed through polymerase chain reaction (PCR) products and sequencing analysis. The plasmids, which contain ads and/or p19 genes, were transformed into Agrobacterium tumefaciens, and then inserted into leaves and hairy roots of A. annua by vacuum and syringe infiltration methods. The successful transformation was checked through the GUS histochemical test and the PCR analysis. Artemisinin levels were measured using HPLC. Results: The percentages of the blue area on leaves by using vacuum and syringe infiltration method and on hairy roots were up to 98, 92.55%, and 99.00% respectively. The ads-p19 sample contained a higher level of artemisinin (0.18%) compared to other samples. Transformed hairy root with co-transformation of ads-p19 contained 0.095% artemisinin, where no artemisinin was found in the control hairy root. The transformation of ads and p19 genes into A. annua plant has been successfully done and could enhance the artemisinin content on the transformed leaves with ads-p19 up to 2.57 folds compared to the untransformed leaves, while for p19, cotransformed and ads were up to 2.25, 1.29, and 1.14 folds respectively. Conclusion: Antisilencing p19 gene could enhance the transformation efficiency of ads and artemisinin level in A. annua.

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MicroRNA Profiles in Monocyte-Derived Macrophages Generated by Interleukin-27 and Human Serum: Identification of a Novel HIV-Inhibiting and Autophagy-Inducing MicroRNA

International Journal of Molecular Sciences ◽

10.3390/ijms22031290 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1290

Author(s):

Tomozumi Imamichi ◽

Suranjana Goswami ◽

Xiaojun Hu ◽

Sylvain Laverdure ◽

Jun Yang ◽

...

Keyword(s):

Innate Immune ◽

Pcr Analysis ◽

Sequencing Analysis ◽

Adaptive Immune ◽

Interleukin 27 ◽

Autophagy Induction ◽

Immunodeficiency Virus ◽

Mirna Sequencing ◽

Polymerase Chain ◽

Adaptive Immune Systems

Interleukin-27 (IL-27) is a pleiotropic cytokine that influences the innate and adaptive immune systems. It inhibits viral infection and regulates the expression of microRNAs (miRNAs). We recently reported that macrophages differentiated from human primary monocytes in the presence of IL-27 and human AB serum resisted human immunodeficiency virus (HIV) infection and showed significant autophagy induction. In the current study, the miRNA profiles in these cells were investigated, especially focusing on the identification of novel miRNAs regulated by IL-27-treatment. The miRNA sequencing analysis detected 38 novel miRNAs. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed that IL-27 differentially regulated the expression of 16 of the 38 miRNAs. Overexpression of the synthesized miRNA mimics by transfection revealed that miRAB40 had potent HIV-inhibiting and autophagy-inducing properties. B18R, an interferon (IFN)-neutralization protein, partially suppressed both activities, indicating that the two functions were induced via IFN-dependent and -independent pathways. Although the target mRNA(s) of miRAB40 involving in the induction of both functions was unable to identify in this study, the discovery of miRAB40, a potential HIV-inhibiting and autophagy inducing miRNA, may provide novel insights into the miRNA (small none-coding RNA)-mediated regulation of HIV inhibition and autophagy induction as an innate immune response.

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Advances in research on genetic relationships of waterfowl parvoviruses

Journal of Veterinary Research ◽

10.2478/jvetres-2021-0063 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Yanhui Chen ◽

Ruth Afumba ◽

Fusheng Pang ◽

Rongxin Yuan ◽

Hao Dong

Keyword(s):

Clinical Symptoms ◽

Genetic Relationships ◽

Eastern China ◽

Economic Losses ◽

Muscovy Duck ◽

Mortality And Morbidity ◽

Causative Agents ◽

Goose Parvovirus ◽

Short Beak ◽

Muscovy Duck Parvovirus

Abstract Derzsy’s disease and Muscovy duck parvovirus disease have become common diseases in waterfowl culture in the world and their potential to cause harm has risen. The causative agents are goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), which can provoke similar clinical symptoms and high mortality and morbidity rates. In recent years, duck short beak and dwarfism syndrome has been prevalent in the Cherry Valley duck population in eastern China. It is characterised by the physical signs for which it is named. Although the mortality rate is low, it causes stunting and weight loss, which have caused serious economic losses to the waterfowl industry. The virus that causes this disease was named novel goose parvovirus (NGPV). This article summarises the latest research on the genetic relationships of the three parvoviruses, and reviews the aetiology, epidemiology, and necropsy characteristics in infected ducks, in order to facilitate further study.

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Genetic characterization and phylogenetic analysis of Novel Duck-Origin Goose Parvovirus in Anhui Province, Eastern China

10.21203/rs.3.rs-202531/v1 ◽

2021 ◽

Author(s):

Yong Wang ◽

Jianfei Sun ◽

Da Zhang ◽

Xu Guo ◽

Wenhao Shen ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genome Sequencing ◽

Phylogenetic Trees ◽

Genetic Characterization ◽

Eastern China ◽

Anhui Province ◽

Muscovy Duck ◽

Goose Parvovirus ◽

Short Beak ◽

Muscovy Duck Parvovirus

Abstract Recently, a novel duck-origin goose parvovirus (N-GPV) was reported to cause short beak and dwarfism syndrome in ducks. In this study, we performed complete genome sequencing and analyzed three different duck-derived parvoviruses that infected different breeds of ducks. Phylogenetic trees based on gene sequences indicated that they were classical goose parvovirus (C-GPV), Muscovy duck parvovirus (MDPV), and N-GPV, respectively. Furthermore, potential recombination events were found. These results improve our understanding of the diversity of duck-derived parvoviruses in the Anhui province, eastern China, and provide a reference for the prevention of associated diseases.

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