scholarly journals REAL-TIME ASSESSMENT OF MICROWAVE ABLATION EFFICACY BY NIR SPECTROSCOPIC TECHNIQUE

2014 ◽  
Vol 07 (01) ◽  
pp. 1350053 ◽  
Author(s):  
JINZHE ZHAO ◽  
ZHIYU QIAN ◽  
JIA LIU ◽  
YA XU ◽  
JIALI WU
Keyword(s):  
Real Time ◽  
Liver Tissue ◽  
Microwave Ablation ◽  
Near Infrared ◽  
Optical Measurement ◽  
Optical Parameter ◽  
Assessment Method ◽  
Efficacy Evaluation ◽  
Time Assessment

Microwave ablation (MWA) status monitoring in real time plays a key role in assessment of therapeutic effectiveness. As a novel real-time assessment method, near infrared spectroscopy (NIRs) was used to evaluate the ablation efficacy. MWA experiments were carried out on in vitro porcine livers. An optical measurement system for biological tissue is developed by our lab to monitor reduced scattering coefficient [Formula: see text] at 690 nm of the coagulation zones. It is noted that [Formula: see text] of liver tissue, which increases as the liver tissue being ablated, is clearly related with the coagulation status. [Formula: see text] of normal tissue and coagulated tissue is 3–5 and 17–19 cm-1, respectively. Continuous changes of [Formula: see text] demonstrate that optical parameter can be used as an efficacy evaluation factor because it essentially indicates the degree of thermal damage. Compared with temperature, optical parameter is more sensitive and accurate, which is promising for real-time therapeutic efficacy assessment in MWA.

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

scholarly journals NIR-Responsive Lysosomotropic Phototrigger: ʽAIE + ESIPTʼ Active Naphthalene Based Single Component Photoresponsive Nanocarrier with Two-Photon Uncaging and Real-Time Monitoring Ability

2021 ◽  
Author(s):  
Biswajit Roy ◽  
Rakesh Mengji ◽  
Samrat Roy ◽  
Bipul Pal ◽  
Avijit Jana ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Single Component ◽  
Photon Absorption ◽  
Real Time Monitoring ◽  
Two Photon ◽  
Nir Light

In recent times, organelle-targeted drug delivery systems gained tremendous attention due to the site specific delivery of active drug molecules resulting in enhanced bioefficacy. In this context, the phototriggered drug delivery system (DDS) for releasing an active molecule is superior as it provides spatial and temporal control over the release. So far, near infrared (NIR) light responsive organelle targeted DDS has not yet been developed. Hence, we introduced a two-photon NIR-light responsive lysosome targeted ʽAIE + ESIPTʼ active single component DDS based on naphthalene chromophore. The Two-photon absorption cross-section of our DDS is 142 GM at 850 nm. The DDS was converted into pure organic nanoparticles for biological applications. Our nano-DDS is capable of selective targeting, AIE-luminogenic imaging, and drug release within the lysosome. In vitro studies using cancerous cell lines showed that our single component photoresponsive nanocarrier exhibited enhanced cytotoxicity and real-time monitoring ability of the drug release.

scholarly journals A New 3D Cultured Liver Chip and Real-Time Monitoring System Based on Microfluidic Technology

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1118
Author(s):  
Yao Zhang ◽  
Ning Yang ◽  
Liangliang Xie ◽  
Fangyu Shu ◽  
Qian Shi ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Monitoring System ◽  
Near Infrared Spectroscopy ◽  
In Vitro Model ◽  
Near Infrared ◽  
Real Time Monitoring ◽  
Microfluidic Technology ◽  
Vitro Model

In vitro models of the liver have a good simulation of the micro-liquid environment inside the human liver and the communication between cell tissues, which provides an important research tool for drug research and liver disease treatment. In this paper, we designed a 3D liver chip and real-time monitoring system based on microfluidic technology. The in vitro model of the liver on the chip was established by the three-dimensional microfluidic chip pipeline and the corresponding microwell array. Meanwhile, the culture medium is continuously injected on the chip, and the electrochemical impedance spectroscopy and near-infrared spectroscopy of the liver chip are recorded and analyzed from day one to day five. When the 3D cultured liver chip in vitro model reached a certain period and stabilized, paracetamol with varying gradients of concentration was applied to the cultured cells for drug resistance testing. The experimental results show that the liver chip and its monitoring system designed in this paper can maintain 100% cell viability of hepatocytes in vitro for a long time. Furthermore, it can meet the requirements of measurement technologies such as electrical impedance measurement and near-infrared spectroscopy in real-time, providing a stable culture platform for the further study of organ chips.

An In-vitro Model to Study Device-induced Thrombosis and Embolism:

2000 ◽  
Vol 83 (02) ◽  
pp. 322-326 ◽  
Author(s):  
Sivaprasad Sukavaneshvar ◽  
Syed Mohammad ◽  
Kenneth Solen
Keyword(s):  
Light Scattering ◽  
Real Time ◽  
In Vitro Model ◽  
Pharmacological Intervention ◽  
Tetraacetic Acid ◽  
Non Invasive ◽  
Prototype Device ◽  
Vitro Model ◽  
Time Assessment

SummaryA bovine in-vitro model was developed to investigate device-induced thromboembolism (TE) and its pharmacological intervention, using a stent as a prototype device. Emboli were assessed continuously using a light-scattering microemboli detector (LSMD). Thrombus on the stent was assessed gravimetrically at the end of the experiment. The contribution of the stent as the predominant source of detectable thromboemboli in this model was verified by placing LSMD probes upstream and downstream of the stent. The effectiveness of ethylenedinitrilo-tetraacetic-acid (EDTA) and three anti-thrombogenic agents (aspirin, dipyridamole, and tirofiban) for mitigating device-induced TE was also assessed. The results show that 1) the model has potential to study device-induced TE and the efficacy of possible interventional strategies, 2) the LSMD is capable of continuous, non-invasive, real-time assessment of embolism, 3) the assessment of embolization may constitute an important part of evaluating hemocompatibility, 4) tirofiban is effective in reducing both stent-induced thrombosis and embolism above certain concentrations.

scholarly journals Noncontact Optical Measurement of Aqueous Humor Glucose Levels and Correlation with Serum Glucose Levels in Rabbit

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 387
Author(s):  
Yih-Shiou Hwang ◽  
Eugene Yu-Chuan Kang ◽  
Chia-Rui Shen ◽  
Wei-Hsin Hong ◽  
Wei-Chi Wu
Keyword(s):  
Aqueous Humor ◽  
Optical System ◽  
Near Infrared ◽  
Optical Measurement ◽  
Serum Glucose ◽  
Mean Difference ◽  
Optical Measurements ◽  
Glucose Levels

The noninvasive measurement of serum glucose levels has been investigated for the monitoring of blood sugar control in diabetes. In our study, we aimed to develop a novel noncontact glucometer (NCGM) utilizing an optical approach to measure the intraocular aqueous humor glucose levels in the anterior chamber of rabbit eyes. The NCGM consists of a hybrid optical system that simultaneously measures near-infrared absorption and the polarized rotatory distribution of glucose molecules in the aqueous humor. In vitro optical measurements demonstrated that NCGM measurements had high precision and repeatability for different glucose levels, including 50 mg/dL (14.36%), 100 mg/dL (−4.05%), 200 mg/dL (−5.99%), 300 mg/dL (4.86%), 400 mg/dL (−2.84%), 500 mg/dL (−0.11%), and 600 mg/dL (4.48%). In the rabbit experiments, we found a high correlation between aqueous glucose levels and serum glucose levels, with a mean difference of 8 mg/dL. According to the testing results, the in vivo NCGM measurement of aqueous humor glucose levels also displayed a high correlation with serum glucose levels, with a mean difference of 29.2 mg/dL. In conclusion, aqueous humor glucose levels were accurately measured using the NCGM, and the results correlated with serum glucose levels.

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