Nonaqueous Solvatochromatic Behavior of Nile Blue A Perchlorate in Imidazolium-Exchanged Clays and its Implication Toward Exfoliation in Polymeric Composites

Sections of osmium-fixed tissues embedded in epon 812 colour with either Sudan black B or Nile blue A solutions to reveal a variety of detail by direct microscopy with normal apochromatic or semi-apochromatic objectives. The clarity of the coloration gives a picture fully comparable to that seen by phase-contrast microscopy. The plastic is not removed, 1-µ sections or thinner sections down to green or gold, are mounted on clean glass slides by drying down from 20% acetone/water after flattening on a hot plate. Colouring is carried out at room temperature in Sudan black B (saturated solution in 70% alcohol) for 1 to 2 h. The result is a reversed or negative effect, for the epon plastic takes the stain avidly, but dense elements of the tissue do not, and appear white against a blue background of stained plastic. Lipid droplets retain a capacity to colour, becoming dark blue to blue-black. Nile blue sulphate ( 1% aqueous solution colours thin sections of tissue in 1 to 2 h at 60° C, acting apparently as a basic dye on most cell elements, and also colours lipid droplets dark blue. After both techniques the sections are mounted in Farrants's medium.

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Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A

Water Science & Technology ◽

10.2166/wst.2011.707 ◽

2011 ◽

Vol 64 (3) ◽

pp. 747-753 ◽

Cited By ~ 12

Author(s):

M. Oshiki ◽

H. Satoh ◽

T. Mino

Keyword(s):

Activated Sludge ◽

Wastewater Treatment Plants ◽

Nile Blue ◽

Maximum Level ◽

Fluorescence Measurement ◽

Batch Reactors ◽

Floc Size ◽

Nile Blue A ◽

On Line ◽

Rapid Quantification

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment plants were stained with Nile blue A and their fluorescence intensities were determined. There was a high correlation (R2 > 0.97) between the fluorescence intensities of Nile blue A and PHA concentrations in activated sludge determined by gas chromatography. The Nile blue A staining and fluorescence measurement method allows us to determine PHA concentrations in activated sludge within only five minutes and up to 96 samples can be measured at once by using microplate reader. On-line monitoring of PHA concentrations in activated sludge was achieved by using a fluorometer equipped with a flow cell and the time point at which PHA concentration in activated sludge reached the maximum level could be identified. In addition, we examined the influence of pH, floc size and co-existing chemicals in activated sludge suspension on the fluorescence intensities of Nile blue A.

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A glucose biosensor based on electrocatalytic oxidation of NADPH at single-walled carbon nanotubes functionalized with poly(nile blue A)

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2008.07.031 ◽

2008 ◽

Vol 624 (1-2) ◽

pp. 21-26 ◽

Cited By ~ 46

Author(s):

Pan Du ◽

Ping Wu ◽

Chenxin Cai

Keyword(s):

Carbon Nanotubes ◽

Electrocatalytic Oxidation ◽

Nile Blue ◽

Single Walled Carbon Nanotubes ◽

Glucose Biosensor ◽

Nile Blue A ◽

Single Walled Carbon ◽

Walled Carbon Nanotubes

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Kinetic determination of trace levels of selenium(IV) and total selenium by Nile Blue A/hydrogen peroxide method

Microchimica Acta ◽

10.1007/bf01242188 ◽

1998 ◽

Vol 128 (1-2) ◽

pp. 43-48 ◽

Cited By ~ 2

Author(s):

Gordana A. Milovanović ◽

Radivoj B. Petronijević ◽

Mira M. Čakar

Keyword(s):

Hydrogen Peroxide ◽

Nile Blue ◽

Kinetic Determination ◽

Nile Blue A ◽

Total Selenium ◽

Peroxide Method

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Evaluation of fipronil baits against Microtermes mycophagus (Blattodea: Termitidae)

The Canadian Entomologist ◽

10.4039/tce.2015.56 ◽

2015 ◽

Vol 148 (3) ◽

pp. 343-352 ◽

Cited By ~ 3

Author(s):

Naeem Iqbal ◽

Theodore A. Evans ◽

Shafqat Saeed ◽

Hafiz Azhar Ali Khan

Keyword(s):

Active Ingredient ◽

Nile Blue ◽

Subterranean Termite ◽

Foraging Activity ◽

Nile Blue A ◽

Toilet Paper ◽

Bait Station ◽

Feasible Alternative

AbstractWe evaluated the efficacy of fipronil baits in suppressing or eliminating field colonies of Microtermes mycophagus (Desneux) (Blattodea: Termitidae) an important subterranean termite pest in Pakistan. We tested two doses (10 and 30 ppm) of fipronil in toilet paper baits, chosen from laboratory repellency tests. We monitored four colonies for foraging activity for one month before baiting, and mapped foraging territories with termites marked with Nile Blue A and agonistic tests. Before the fipronil baits were installed there were averages of 782–1938 workers and soldiers per bait station in the four colonies. After baiting, the colonies were eliminated as there were no workers per bait station, whereas the control colony had an average of 1142 workers per bait station. The three possibly eliminated colonies consumed around 47 mg of fipronil formulation (4.7 mg active ingredient) in 45–90 days. Our results suggest that baits containing fipronil could provide an economical and feasible alternative for the management of M. mycophagus in structures and buildings in Pakistan.

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