Temperature-controlled perfusion apparatus for microscope using transparent conducting film heater

1989 ◽  
Vol 256 (1) ◽  
pp. C214-C217 ◽  
Author(s):  
S. Toyotomi ◽  
Y. Momose

We describe a novel temperature-controlled perfusion apparatus for electrophysiological studies on isolated cells or membrane patches. The apparatus uses a transparent conducting film of indium-tin-oxide as a heating element. The film heater deposited on a glass sheet allows us to construct a structurally simple apparatus that is able not only to heat directly the bath chamber but also to preheat the perfusion solution on the stage of an inverted microscope. The structure ensures a uniform temperature in a bath chamber that has a working area of approximately 6 X 12 mm2 and a capacity of 1 ml. A control unit, designed for operation of the apparatus, regulates the temperature of the bathing solution without introducing electrical noise in a range from ambient temperature to greater than 37 degrees C with an accuracy within +/- 1 degree C. The apparatus is 12 mm in height and designed to fit on the stage of a standard inverted microscope. Since the bath chamber for perfusion experiments is readily interchanged with others, the apparatus can be used widely in microscopic studies of various cells in static solutions.

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yuan-Jun Song ◽  
Jing Chen ◽  
Jing-Yuan Wu ◽  
Tong Zhang

Silver nanowire has potential applications on transparent conducting film and electrode of electrochemical capacitor due to its excellent conductivity. Transparent conducting film (G-film) was prepared by coating silver nanowires on glass substrate using Meyer rod method, which exhibited better performance than carbon nanotube and graphene. The conductivity of G-film can be improved by increasing sintering temperature. Electrode of electrochemical capacitor (I-film) was fabricated through the same method with G-film on indium tin oxide (ITO). CV curves of I-film under different scanning rates had obvious redox peaks, which indicated that I-film exhibited excellent electrochemical pseudocapacitance performance and good reversibility during charge/discharge process. In addition, the specific capacitance of I-film was measured by galvanostatic charge/discharge experiments, indicating that I-film exhibits high special capacitance and excellent electrochemical stability.


RSC Advances ◽  
2017 ◽  
Vol 7 (26) ◽  
pp. 16139-16148 ◽  
Author(s):  
Molla Bahiru Gebeyehu ◽  
Tolesa Fita Chala ◽  
Shao-Yen Chang ◽  
Chang-Mou Wu ◽  
Jiunn-Yih Lee

The high quality transparent conducting film (TCF) at a low sheet resistance of uniform and purified silver nanowires (AgNWs) have been successfully produced, the optoelectronic performance, which exceeds that of indium tin oxide (ITO).


2011 ◽  
Vol 34 (4) ◽  
pp. 623-628 ◽  
Author(s):  
Tsuyoshi Saotome ◽  
Hansang Kim ◽  
Zhe Wang ◽  
David Lashmore ◽  
H. Thomas Hahn

Nanoscale ◽  
2016 ◽  
Vol 8 (7) ◽  
pp. 3916-3922 ◽  
Author(s):  
Hyeon-Gyun Im ◽  
Byeong Wan An ◽  
Jungho Jin ◽  
Junho Jang ◽  
Young-Geun Park ◽  
...  

A junction resistance-free continuous metal nanotrough-embedded transparent conducting electrode (TCE) composite film (metal nanotrough-GFRHybrimer film) is reported.


2009 ◽  
Vol 20 (47) ◽  
pp. 475703 ◽  
Author(s):  
Dong-Wook Shin ◽  
Jong Hak Lee ◽  
Yu-Hee Kim ◽  
Seong Man Yu ◽  
Seong-Yong Park ◽  
...  

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