Anti-inflammatory effects ofSaccharomyces boulardiimediated by myeloid dendritic cells from patients with Crohn's disease and ulcerative colitis

Saccharomyces boulardii ( Sb) is a probiotic yeast that has demonstrated efficacy in pilot studies in patients with inflammatory bowel disease (IBD). Microbial antigen handling by dendritic cells (DC) is believed to be of critical importance for immunity and tolerance in IBD. The aim was to characterize the effects of Sb on DC from IBD patients. Highly purified (>95%), lipopolysaccharide-stimulated CD1c+CD11c+CD123−myeloid DC (mDC) from patients with ulcerative colitis (UC; n = 36), Crohn's disease (CD; n = 26), or infectious controls (IC; n = 4) were cultured in the presence or absence of fungal supernatant from Sb ( SbS). Phenotype and cytokine production and/or secretion of IBD mDC were measured by flow cytometry and cytometric bead arrays, respectively. T cell phenotype and proliferation were assessed in a mixed lymphocyte reaction (MLR) with allogenic CD4+CD45RA+naïve T cells from healthy donors. Mucosal healing was investigated in epithelial wounding and migration assays with IEC-6 cells. SbS significantly decreased the frequency of CD40-, CD80-, and CD197 (CCR7; chemokine receptor-7)-expressing IBD mDC and reduced their secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-6 while increasing IL-8. In the MLR, SbS significantly inhibited T cell proliferation induced by IBD mDC. Moreover, SbS inhibited TH1 (TNF-α and interferon-γ) polarization induced by UC mDC and promoted IL-8 and transforming growth factor-β-dependent mucosal healing. In summary, we provide novel evidence of synergistic mechanisms how Sb controls inflammation (inhibition of T cell costimulation and inflammation-associated migration and mobilization of DC) and promotes epithelial restitution relevant in IBD.

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Anti-Inflammatory Effects of Saccharomyces Boulardii on Myeloid Dendritic Cells From Patients With Crohn's Disease and Ulcerative Colitis

Gastroenterology ◽

10.1016/s0016-5085(11)63526-9 ◽

2011 ◽

Vol 140 (5) ◽

pp. S-849

Author(s):

Saskia Thomas ◽

Diana Metzke ◽

Yvonne Dörffel ◽

Jürgen Schmitz ◽

Andreas Radbruch ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dendritic Cells ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Saccharomyces Boulardii ◽

Myeloid Dendritic Cells ◽

Anti Inflammatory

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Anti-inflammatory effects of Saccharomyces boulardii mediated by myeloid dendritic cells from patients with Crohn's disease and ulcerative colitis

Zeitschrift für Gastroenterologie ◽

10.1055/s-0031-1285373 ◽

2011 ◽

Vol 49 (08) ◽

Author(s):

S Thomas ◽

D Metzke ◽

J Schmitz ◽

Y Dörffel ◽

A Radbruch ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dendritic Cells ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Saccharomyces Boulardii ◽

Myeloid Dendritic Cells ◽

Anti Inflammatory

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Probiotic Lactobacillus and Bifidobacterium Strains Counteract Adherent-Invasive Escherichia coli (AIEC) Virulence and Hamper IL-23/Th17 Axis in Ulcerative Colitis, but Not in Crohn’s Disease

Cells ◽

10.3390/cells9081824 ◽

2020 ◽

Vol 9 (8) ◽

pp. 1824

Author(s):

Gabriella Leccese ◽

Alessia Bibi ◽

Stefano Mazza ◽

Federica Facciotti ◽

Flavio Caprioli ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dendritic Cells ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Immune Cells ◽

Intestinal Epithelial ◽

Healthy Donors ◽

Tnf Α ◽

Bowel Diseases ◽

Probiotic Strains

Hypersecretion of proinflammatory cytokines and dysregulated activation of the IL-23/Th17 axis in response to intestinal microbiota dysbiosis are key factors in the pathogenesis of inflammatory bowel diseases (IBD). In this work, we studied how Lactobacillus and Bifidobacterium strains affect AIEC-LF82 virulence mechanisms and the consequent inflammatory response linked to the CCR6–CCL20 and IL-23/Th17 axes in Crohn’s disease (CD) and ulcerative colitis (UC) patients. All Lactobacillus and Bifidobacterium strains significantly reduced the LF82 adhesion and persistence within HT29 intestinal epithelial cells, inhibiting IL-8 secretion while not affecting the CCR6–CCL20 axis. Moreover, they significantly reduced LF82 survival within macrophages and dendritic cells, reducing the secretion of polarizing cytokines related to the IL-23/Th17 axis, both in healthy donors (HD) and UC patients. In CD patients, however, only B. breve Bbr8 strain was able to slightly reduce the LF82 persistence within dendritic cells, thus hampering the IL-23/Th17 axis. In addition, probiotic strains were able to modulate the AIEC-induced inflammation in HD, reducing TNF-α and increasing IL-10 secretion by macrophages, but failed to do so in IBD patients. Interestingly, the probiotic strains studied in this work were all able to interfere with the IL-23/Th17 axis in UC patients, but not in CD patients. The different interaction mechanisms of probiotic strains with innate immune cells from UC and CD patients compared to HD suggest that testing on CD-derived immune cells may be pivotal for the identification of novel probiotic strains that could be effective also for CD patients.

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Su1865 PANETH CELL PHENOTYPE AT ILEAL RESECTION MARGIN OBTAINED AT TIME OF ILEOCOLECTOMY FOR ULCERATIVE COLITIS CORRELATES WITH DE NOVO CROHN'S DISEASE AND TIME TO POUCHITIS DEVELOPMENT

Gastroenterology ◽

10.1016/s0016-5085(20)32392-1 ◽

2020 ◽

Vol 158 (6) ◽

pp. S-680-S-681

Author(s):

Anas K. Gremida ◽

Matthew A. Ciorba ◽

Ta-Chiang Liu

Keyword(s):

Ulcerative Colitis ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Resection Margin ◽

De Novo ◽

Paneth Cell ◽

Cell Phenotype ◽

Ileal Resection

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Therapeutic effect of anti-OX40L and anti-TNF-α MAbs in a murine model of chronic colitis

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00450.2002 ◽

2003 ◽

Vol 284 (4) ◽

pp. G595-G603 ◽

Cited By ~ 38

Author(s):

T. Totsuka ◽

T. Kanai ◽

K. Uraushihara ◽

R. Iiyama ◽

M. Yamazaki ◽

...

Keyword(s):

T Cells ◽

Crohn’S Disease ◽

T Cell ◽

Crohn's Disease ◽

Lamina Propria ◽

Therapeutic Effect ◽

Therapeutic Effects ◽

Chronic Colitis ◽

Tnf Α ◽

Ifn Γ

Interaction of OX40 (CD134) on T cells with its ligand (OX40L) on antigen-presenting cells has been implicated in pathogenic T cell activation. This study was performed to explore the involvement of OX40/OX40L in the development of T cell-mediated chronic colitis. We evaluated both the preventive and therapeutic effects of neutralizing anti-OX40L MAb on the development of chronic colitis in SCID mice induced by adoptive transfer of CD4+CD45RBhighT cells as an animal model of Crohn's disease. We also assessed the combination of anti-OX40L and anti-TNF-α MAbs to improve the therapeutic effect. Administration of anti-OX40L MAb markedly ameliorated the clinical and histopathological disease in preventive and therapeutic protocols. In vivo treatment with anti-OX40L MAb decreased CD4+T cell infiltration in the colon and suppressed IFN-γ, IL-2, and TNF-α production by lamina propria CD4+T cells. The combination with anti-TNF-α MAb further improved the therapeutic effect by abolishing IFN-γ, IL-2, and TNF-α production by lamina propria CD4+T cells. Our present results suggested a pivotal role of OX40/OX40L in the pathogenesis of T cell-mediated chronic colitis. The OX40L blockade, especially in combination with the TNF-α blockade, may be a promising strategy for therapeutic intervention of Crohn's disease.

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