Increased myocardial lactate oxidation in lambs with aortopulmonary shunts at rest and during exercise

1998 ◽  
Vol 275 (5) ◽  
pp. H1503-H1512 ◽  
Author(s):  
Gertie C. M. Beaufort-Krol ◽  
Janny Takens ◽  
Marieke C. Molenkamp ◽  
Gioia B. Smid ◽  
Koos J. Meuzelaar ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Myocardial Hypertrophy ◽  
Conscious State ◽  
Exercise Group ◽  
Lactate Oxidation ◽  
Lactate Release ◽  
Exogenous Glucose ◽  
Lactate Uptake ◽  
And Control ◽  
Myocardial Oxidative Metabolism

Free fatty acids are the major fuels for the myocardium, but during a higher load carbohydrates are preferred. Previously, we demonstrated that myocardial net lactate uptake was higher in lambs with aortopulmonary shunts than in control lambs. To determine whether this was caused by an increased lactate uptake and oxidation or by a decreased lactate release, we studied myocardial lactate and glucose metabolism with13C-labeled substrates in 36 lambs in a fasting, conscious state. The lambs were assigned to two groups: a resting group consisting of 8 shunt and 9 control lambs, and an exercise group (50% of peak O2consumption) consisting of 9 shunt and 10 control lambs. Myocardial lactate oxidation was higher in shunt than in control lambs (mean ± SE, rest: 10.33 ± 2.61 vs. 0.17 ± 0.82, exercise: 38.05 ± 8.87 vs. 16.89 ± 4.78 μmol ⋅ min−1⋅ 100 g−1; P < 0.05). There was no difference in myocardial lactate release between shunt and control lambs. Oxidation of exogenous glucose, which was approximately zero at rest, increased during exercise in shunt and control lambs. The contribution of glucose and lactate to myocardial oxidative metabolism increased during exercise compared with at rest in both shunt and control lambs. We conclude that myocardial lactate oxidation is higher in shunt than in control lambs, both at rest and during exercise, and that the contribution of carbohydrates in myocardial oxidative metabolism in shunt lambs is higher than in control lambs. Thus it appears that this higher contribution of carbohydrates occurs not only in the case of pressure-overloaded hearts but also in myocardial hypertrophy due to volume overloading.

scholarly journals Similar carbohydrate but enhanced lactate utilization during exercise after 9 wk of acclimatization to 5,620 m

2002 ◽  
Vol 283 (6) ◽  
pp. E1203-E1213 ◽  
Author(s):  
G. van Hall ◽  
J. A. L. Calbet ◽  
H. Søndergaard ◽  
B. Saltin
Keyword(s):  
Sea Level ◽  
Glucose Utilization ◽  
Whole Body ◽  
Glucose Disposal ◽  
Bicycle Exercise ◽  
Lactate Oxidation ◽  
Lactate Release ◽  
Relative Contribution ◽  
Lactate Uptake ◽  
Lactate Utilization

We hypothesized that reliance on lactate as a means of energy distribution is higher after a prolonged period of acclimatization (9 wk) than it is at sea level due to a higher lactate Ra and disposal from active skeletal muscle. To evaluate this hypothesis, six Danish lowlanders (25 ± 2 yr) were studied at rest and during 20 min of bicycle exercise at 146 W at sea level (SL) and after 9 wk of acclimatization to 5,260 m (Alt). Whole body glucose Ra was similar at SL and Alt at rest and during exercise. Lactate Ra was also similar for the two conditions at rest; however, during exercise, lactate Ra was substantially lower at SL (65 μmol · min−1 · kg body wt−1) than it was at Alt (150 μmol · min−1 · kg body wt−1) at the same exercise intensity. During exercise, net lactate release was ∼6-fold at Alt compared with SL, and related to this, tracer-calculated leg lactate uptake and release were both 3- or 4-fold higher at Alt compared with SL. The contribution of the two legs to glucose disposal was similar at SL and Alt; however, the contribution of the two legs to lactate Ra was significantly lower at rest and during exercise at SL (27 and 81%) than it was at Alt (45 and 123%). In conclusion, at rest and during exercise at the same absolute workload, CHO and blood glucose utilization were similar at SL and at Alt. Leg net lactate release was severalfold higher, and the contribution of leg lactate release to whole body lactate Ra was higher at Alt compared with SL. During exercise, the relative contribution of lactate oxidation to whole body CHO oxidation was substantially higher at Alt compared with SL as a result of increased uptake and subsequent oxidation of lactate by the active skeletal muscles.

Contribution of oxidative metabolism and glycolysis to ATP production in hypertrophied hearts

1994 ◽  
Vol 267 (2) ◽  
pp. H742-H750 ◽  
Author(s):  
M. F. Allard ◽  
B. O. Schonekess ◽  
S. L. Henning ◽  
D. R. English ◽  
G. D. Lopaschuk
Keyword(s):  
Oxidative Metabolism ◽  
Work Conditions ◽  
Primary Energy ◽  
Acid Oxidation ◽  
Lactate Oxidation ◽  
Atp Production ◽  
Palmitate Oxidation ◽  
Rat Hearts ◽  
14Co2 Production ◽  
And Control

The contribution of glycolysis and oxidative metabolism to ATP production was determined in isolated working hypertrophied hearts perfused with Krebs-Henseleit buffer containing 3% albumin, 0.4 mM palmitate, 0.5 mM lactate, and 11 mM glucose. Glycolysis and glucose oxidation were directly measured by perfusing hearts with [5–3H/U-14C]glucose and by measuring 3H2O and 14CO2 production, respectively. Palmitate and lactate oxidation were determined by simultaneous measurement of 3H2O and 14CO2 in hearts perfused with [9,10–3H]palmitate and [U-14C]lactate. At low workloads (60 mmHg aortic after-load), rates of palmitate oxidation were 47% lower in hypertrophied hearts than in control hearts, but palmitate oxidation remained the primary energy source in both groups, accounting for 55 and 69% of total ATP production, respectively. The contribution of glycolysis to ATP production was significantly higher in hypertrophied hearts (19%) than in control hearts (7%), whereas that of glucose and lactate oxidation did not differ between groups. During conditions of high work (120 mmHg aortic afterload), the extra ATP production required for mechanical function was obtained primarily from an increase in the oxidation of glucose and lactate in both groups. The contribution of palmitate oxidation to overall ATP production decreased in hypertrophied and control hearts (to 40 and 55% of overall ATP production, respectively) and was no longer significantly depressed in hypertrophied hearts. Glycolysis, on the other hand, was accelerated in control hearts to rates seen in the hypertrophied hearts. Thus a reduced contribution of fatty acid oxidation to energy production in hypertrophied rat hearts is accompanied by a compensatory increase in glycolysis during low work conditions.(ABSTRACT TRUNCATED AT 250 WORDS)

Recovery of glycolysis and oxidative metabolism during postischemic reperfusion of hypertrophied rat hearts

1996 ◽  
Vol 271 (2) ◽  
pp. H798-H805 ◽  
Author(s):  
B. O. Schonekess ◽  
M. F. Allard ◽  
G. D. Lopaschuk
Keyword(s):  
Oxidative Metabolism ◽  
Glucose Oxidation ◽  
Mechanical Function ◽  
Lactate Oxidation ◽  
Atp Production ◽  
Postischemic Reperfusion ◽  
Palmitate Oxidation ◽  
Abdominal Aortic ◽  
Rat Hearts ◽  
And Control

We investigated the source and extent of recovery of ATP production during postischemic reperfusion of isolated working hearts from abdominal aortic-banded rats. Rates of glycolysis, glucose oxidation, lactate oxidation, and palmitate oxidation were measured in hypertrophied and control hearts [perfused with (in mM) 11 glucose, 0.5 lactate, and 1.2 palmitate] during and after 30 min of no-flow ischemia. In the initial aerobic period glycolytic rates were 1.87-fold higher in hypertrophied hearts compared with control hearts (P < 0.05), with rates of carbohydrate and palmitate oxidation being similar. During reperfusion, hypertrophied hearts recovered 40% of preischemic function compared with 71% in control hearts. Rates of glycolysis during reperfusion of hypertrophied hearts remained accelerated compared with control hearts (2.01-fold higher, P < 0.05), whereas oxidative metabolism returned to preischemic values in both groups. The efficiency of converting ATP production into mechanical work decreased to 29% of preischemic values in hypertrophied hearts during the postischemic reperfusion compared with a decrease to only 59% of preischemic values in control hearts. This suggests that the recovery of glycolysis and oxidative metabolism in the hypertrophied heart during postischemic reperfusion is not impaired, but rather the efficiency of converting ATP produced into mechanical function decreases.

scholarly journals C-11 acetate has excellent reproducibility for quantification of myocardial oxidative metabolism

2014 ◽  
Vol 16 (5) ◽  
pp. 500-506 ◽  
Author(s):  
S. V. Nesterov ◽  
O. Turta ◽  
C. Han ◽  
M. Maki ◽  
I. Lisinen ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Myocardial Oxidative Metabolism ◽  
Excellent Reproducibility

scholarly journals Leg and arm lactate and substrate kinetics during exercise

2003 ◽  
Vol 284 (1) ◽  
pp. E193-E205 ◽  
Author(s):  
G. van Hall ◽  
M. Jensen-Urstad ◽  
H. Rosdahl ◽  
H.-C. Holmberg ◽  
B. Saltin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Mass ◽  
Lactate Concentration ◽  
Whole Body ◽  
Glucose Turnover ◽  
Lactate Oxidation ◽  
Arterial Lactate ◽  
Lactate Uptake ◽  
High Lactate

To study the role of muscle mass and muscle activity on lactate and energy kinetics during exercise, whole body and limb lactate, glucose, and fatty acid fluxes were determined in six elite cross-country skiers during roller-skiing for 40 min with the diagonal stride (Continuous Arm + Leg) followed by 10 min of double poling and diagonal stride at 72–76% maximal O2 uptake. A high lactate appearance rate (Ra, 184 ± 17 μmol · kg−1 · min−1) but a low arterial lactate concentration (∼2.5 mmol/l) were observed during Continuous Arm + Leg despite a substantial net lactate release by the arm of ∼2.1 mmol/min, which was balanced by a similar net lactate uptake by the leg. Whole body and limb lactate oxidation during Continuous Arm + Leg was ∼45% at rest and ∼95% of disappearance rate and limb lactate uptake, respectively. Limb lactate kinetics changed multiple times when exercise mode was changed. Whole body glucose and glycerol turnover was unchanged during the different skiing modes; however, limb net glucose uptake changed severalfold. In conclusion, the arterial lactate concentration can be maintained at a relatively low level despite high lactate Ra during exercise with a large muscle mass because of the large capacity of active skeletal muscle to take up lactate, which is tightly correlated with lactate delivery. The limb lactate uptake during exercise is oxidized at rates far above resting oxygen consumption, implying that lactate uptake and subsequent oxidation are also dependent on an elevated metabolic rate. The relative contribution of whole body and limb lactate oxidation is between 20 and 30% of total carbohydrate oxidation at rest and during exercise under the various conditions. Skeletal muscle can change its limb net glucose uptake severalfold within minutes, causing a redistribution of the available glucose because whole body glucose turnover was unchanged.

scholarly journals El efecto de la terapia acuática en el plasma y la interleucina-12 y 17 en pacientes con esclerosis múltiple

2019 ◽  
Vol 8 (1) ◽  
pp. 89-94
Author(s):  
S. Nejatpour ◽  
M. Fathei ◽  
A. Yaghoubi
Keyword(s):  
Multiple Sclerosis ◽  
Exercise Group ◽  
Interleukin 12 ◽  
Interleukin 17 ◽  
Esclerosis Múltiple ◽  
Intervention Protocol ◽  
Before And After ◽  
Post Test ◽  
Factores De Riesgo ◽  
And Control

Las citocinas como la interleucina-12 (IL-12) y la interleucina-17 (IL-17) influyen en la función del sistema inmune y los diferentes tejidos, siendo estudiadas debido al papel que desempeñan en la Esclerosis Múltiple (EM). El objetivo de este estudio fue investigar el efecto de la terapia acuática en la IL-12 y la IL-17 en pacientes con esclerosis múltiple. Participaron 25 hombres con esclerosis múltiple, los cuales fueron divididos en dos grupos: ejercicio y control. Se tomó una muestra de sangre antes y después de la intervención. El grupo de ejercicio llevó a cabo tres sesiones por semana durante ocho semanas. Las partes inicial y final de cada sesión incluyeron calentamiento y vuelta a la calma, llevadas a cabo en zonas poco profundas. Las actividades fueron diseñadas y llevadas a cabo por un investigador bajo la supervisión de un fisiólogo deportivo y un neurólogo experimentado. Durante las sesiones de entrenamiento, se dedicaban los primeros 10 minutos al calentamiento y los últimos 5 minutos a la vuelta a la calma. La intensidad del ejercicio fue aproximadamente del 75% de la frecuencia cardíaca máxima. Los datos demostraron que tanto IL-12 como IL-17 disminuyeron significativamente entre el pre-test y el post-test. Por tanto, se concluye que la terapia acuática puede reducir los factores de riesgo con respecto a la esclerosis múltiple, incluidas la IL-12 y la IL-17. Cytokines such as Interleukin 12 (IL-12) and Interleukin 17(IL-17) influence the function of the immune system and different tissues and are studied due to the role they play in Multiple Sclerosis (MS). The aim of the study was to investigate the effect of aqua-therapy on plasma IL-12 and IL-17 in patients with MS. 25 men with MS were divided into two groups: exercise and control. Blood sample was taken before and after the intervention protocol. The exercise group carried out three exercise sessions per week during eight weeks. The beginning and final parts of each session included warming up and cool down, and were carried out in shallow areas. These activities were designed and implemented by a researcher under the supervision of a sports physiologist and an experienced neurologist. During the training sessions, the first 10 minutes were for warming up, and the last 5 minutes of training were for cool down. Exercise intensity was approximately 75% of heart rate reserve. The data demonstrated that both IL-12 and IL-17 decreased significantly from pre-test to post-test in the exercise group. We conclude that aqua-training may reduce risk factors regarding multiple sclerosis, including IL-12 and IL-17.

Effects of aerobic plus explosive power exercises on bone remodeling and bone mineral density in young men

2017 ◽  
Vol 43 (1) ◽  
pp. 40-48
Author(s):  
Veysi Çay ◽  
Gurbuz Buyukyazi ◽  
Cevval Ulman ◽  
Fatma Taneli ◽  
Yeliz Doğru ◽  
...  
Keyword(s):  
Maximal Oxygen Consumption ◽  
Exercise Program ◽  
Exercise Group ◽  
Risk Level ◽  
Control Group ◽  
Maximal Heart Rate ◽  
Mineral Density ◽  
Explosive Power ◽  
Before And After ◽  
And Control

AbstractObjectives:This study aims to examine effects of aerobic jogging and explosive power exercises on body composition, maximal oxygen consumption (MaxVOMaterials and methods:Thirty seven healthy males were divided into exercise group (EG, n=19) and control group (CG, n=18). EG completed 10 weeks of outdoor aerobic (jogging at 60–70% maximal heart rate reserve starting from 20 min steadily increasing up to 28 min) and explosive power exercises (in 2–3 sets, with maximum repetitions lasting 15 s), 3 times per week. All measurements were taken before and after the exercise program. BMD was measured via dual energy X-ray absorptiometry (DEXA).Results:Body mass index (p<0.001) significantly decreased; MaxVOConclusion:Performing our outdoor exercise program in spring months might have an important role in the significant increase (9 vs. 22 ng/mL) in mean vitamin D level, which reached above the fracture risk level of 20 ng/mL.

scholarly journals Effect of Fiber Rich Product on Childhood Obesity and Lipid Profile Aged 10-12 Years

2021 ◽  
Vol 9 (3) ◽  
pp. 87-90
Author(s):  
A. Jyoth ◽  
Keyword(s):  
Exercise Group ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Diet Survey ◽  
Group Ii ◽  
And Control ◽  
Experimental Group ◽  
Positive Results ◽  
Green Peas

The effect of fiber rich product on child hood obesity studied with 60 sample which were collected randomly among 10-12 years and categorized into 2 groups i.e, experimental group and control group. Experimental group further categorized into three groups i.e, supplementation with exercise (n=15), only supplementation group (n=15), only exercise group (n=15) and control group consists of (n=15).Anthropometric, and diet survey conducted as parameters. A fiber rich product was prepared and supplemented for 2 months to the selected subjects and it consists of whole Bengal gram, whole green gram, green peas, barley and jaggery. Positive results were obtained in three experimental groups. Significant decrease observed inweight, and BMI, Total cholesterol, Triglycerides, LDL, VLDL significant increase observed in HDL in group I II and III. The results were (51.60-48.26kg, 24.7-23.1, 195.2-152.3 mg/dl, 168.2-145.0 mg/dl, 52.2-54.13 mg/dl, 109.4- 69.4mg/dl, 33.6-28.7mg/dl) in group I, In group II the results are (50.3-49.86kg, 23.7-23.4, 168.6-161.0mg/dl, 145.4-129.6mg/dl, 44.2-45.2 mg/dl, 95.3-90.0mg/dl, 29.1-28.3mg/dl).In group III the tesults aere (50.7-49.6kg, 24.5-23.9, 143.2-139.3mg/dl, 139-134.5mg/dl, 38.2-38.7mg/dl 76.8-74.1 mg/dl, 25.6-28.1mg/dl) .From the results it was clear that when compared to group II and III group I has shown better results.

Abstract 16976: Derangements in Cardiac Oxidative Metabolism in Humans With Dilated Cardiomyopathy

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Daniel Clark ◽  
Giovanni E Davogustto ◽  
Susan P Bell ◽  
RAVINDER MALLUGARI ◽  
William S Bradham ◽  
...  
Keyword(s):  
Myocardial Perfusion ◽  
Dilated Cardiomyopathy ◽  
Oxidative Metabolism ◽  
Myocardial Perfusion Reserve ◽  
Normal Subjects ◽  
Rate Pressure Product ◽  
Gadolinium Contrast ◽  
Perfusion Reserve ◽  
Demand Rate ◽  
Myocardial Oxidative Metabolism

Introduction: Dilated cardiomyopathy (DCM) is associated with impaired myocardial perfusion reserve and impaired myocardial oxidative metabolism. However, the association between myocardial perfusion reserve and oxidative metabolism, is not fully understood. Hypothesis: Reduced myocardial perfusion reserve is associated with reduced myocardial oxidative metabolism. Methods: Using non-invasive cardiac imaging, we studied 8 DCM patients and 14 normal subjects. Myocardial perfusion reserve index (MPRI) was calculated using cardiac magnetic resonance as the normalized rate of myocardial signal augmentation following gadolinium contrast injection between rest and regadenoson induced stress. Resting oxidative metabolism was calculated as the myocardial mono-exponential decay rate (Kmono) of [ 11 C]acetate by positron emission tomography normalized per unit demand (rate-pressure product, RPP) (Kmono/RPP). Results: MPRI was lower in DCM compared to controls (1.25 ± 0.22 vs 1.59 ± 0.49, p=0.038). Similarly, Kmono/RPP was lower in DCM compared with normal subjects (0.6x10e-3 ± 0.15 x10e-3 vs 1.2x10e-3 ± 0.9x10e-3, p<0.0001). There was a linear relation between Kmono and RPP in normal subjects. However, DCM patients showed no increase in Kmono regardless of RPP (Figure 1A). Kmono/RPP was not significantly related to MPRI in either group (Figure 1B). Conclusions: Patients with DCM exhibit markedly impaired myocardial oxidative metabolism compared to normal subjects. However, this impairment was not quantitatively related to impaired myocardial perfusion reserve. Of the various mechanisms that could explain decrease in oxidative metabolism in DCM, these data suggest that reduced myocardial perfusion is not the principal driver of impaired oxidative metabolism.

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