Lipid changes in cardiac hypertrophy as measured by silicic acid chromatography

1963 ◽  
Vol 204 (2) ◽  
pp. 297-300 ◽  
Author(s):  
G. H. Nelson ◽  
D. M. Bear ◽  
T. O. Dotson ◽  
R. F. Krause
Keyword(s):  
Cardiac Hypertrophy ◽  
Silicic Acid ◽  
Total Lipid ◽  
Atmospheric Pressure ◽  
Silicic Acid Column ◽  
Prolonged Exposure ◽  
Phospholipid Content ◽  
Albino Rats ◽  
Silicic Acid Column Chromatography ◽  
Silicic Acid Chromatography

Cardiac hypertrophy was produced in adult, male albino rats by prolonged exposure to reduced atmospheric pressure. Total lipid extracts of the heart were made and the lipids were separated into seven fractions by silicic acid column chromatography. The purity of each fraction was determined by chemical analysis. Fractions II, IV, and VII were found to contain reasonably pure triglyceride, cholesterol, and phospholipid, respectively. Fractions I, III, V, and VI contained cholesterol ester, free fatty acid, diglyceride, and monoglyceride, respectively, but were contaminated with other lipid material. The hypertrophied hearts showed a reduction in percentage of total lipid but no change in the weight of total lipid per heart. As the heart increased in weight the phospholipid content increased and the weight of cholesterol decreased. These findings confirm previous observations that the phospholipid content of muscles increases with activity.

Creatine, creatine phosphate, and creatinine levels in hypoxia hypertrophied rat hearts

1963 ◽  
Vol 204 (6) ◽  
pp. 1005-1007 ◽  
Author(s):  
C. D. Edman ◽  
N. P. Silvers ◽  
E. C. Gangloff ◽  
R. F. Krause
Keyword(s):  
Cardiac Hypertrophy ◽  
Adult Male ◽  
Atmospheric Pressure ◽  
Prolonged Exposure ◽  
Creatine Phosphate ◽  
Albino Rats ◽  
Rat Hearts

Cardiac hypertrophy was produced in adult, male, albino rats by prolonged exposure to reduced atmospheric pressure. Creatine, creatine phosphate, and creatinine levels in these hypertrophied hearts were all significantly reduced from that of control rats.

Microdetermination of Cholesterol and Phospholipid in Cerebrospinal Fluid and Serum by Silicic Acid Column Chromatography

1962 ◽  
Vol 8 (6) ◽  
pp. 598-605 ◽  
Author(s):  
Yung S Shin ◽  
James C Lee
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Serum ◽  
Total Phosphorus ◽  
Silicic Acid ◽  
Column Chromatography ◽  
Silicic Acid Column ◽  
Lipid Phosphorus ◽  
Silicic Acid Column Chromatography ◽  
Phospholipid Ratio

Abstract A method is presented for the determination of cholesterol and phospholipid, which requires 5 µl. of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 µg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 µg. of cholesterol and 30.5 µg. of phospholipid was 98.4 and 96.7%, respectively. Standard deviations of ± 1.73 and ± 1.24 have been obtained for the reproducibility of cholesterol and phospholipid determinations after chromatography. The method has been applied for the estimation of the cholesterol/phospholipid ratio and of lipid phosphorus in total phosphorus of human cerebrospinal fluids.

scholarly journals Studies on the Physico-Chemical Properties of Siyal Kanta (Argemone mexicana linn) Seed Oil

1970 ◽  
Vol 46 (4) ◽  
pp. 561-564 ◽  
Author(s):  
Gm Ahmed ◽  
MS Rahman ◽  
MR Zaman ◽  
MA Hossain ◽  
MM Uddin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Silicic Acid ◽  
Column Chromatography ◽  
Seed Oil ◽  
Silicic Acid Column ◽  
Neutral Lipids ◽  
Chemical Properties ◽  
Silicic Acid Column Chromatography ◽  
Physico Chemical

The physico-chemical properties of the extracted oil were studied by the conventional methods. It was observed that Siyal Kanta grown under the soil and climatic condition of Bangladesh contains about 35% of pale yellow coloured oil. The total lipids were fractionated into three major lipid groups, neutral lipids, glycolipid and phospholipids by silicic acid column chromatography. Among the lipids, the neutral lipids were varied from 92.1-92.3%, glycolipid 5.5-5.8% and phospholipid 1.5-1.7% of the total oil of the lipid applied. The oil was also fractionated into mono-, di- and triglyceride by silicic acid column chromatography. The triglycerides were varied from 90.1-90.3%, diglycerides from 2.3-2.8% and monoglycerides from 1.5-1.8%. The saturated and unsaturated fatty acids present in the oil were separated and found to be 14.2-14.5% and 84.2-84.8% respectively depending on the areas in which the plant grows. The fatty acid compositions of the oil were analyzed by Gas Liquid Chromatography (GLC). The major fatty acids found in the oil were oleic acid (23%), linoleic acid (58%), palmetic acid (7%) and ricinoleic acid (10%). Key words: Siyal kanta seed oil; Glyceride; Lipid; Fatty acid. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9607 BJSIR 2011; 46(4): 561-564

scholarly journals The dietary effect of milk sphingomyelin on the lipid metabolism of obese/diabetic KK-Aymice and wild-type C57BL/6J mice

2016 ◽  
Vol 7 (9) ◽  
pp. 3854-3867 ◽  
Author(s):  
Ippei Yamauchi ◽  
Mariko Uemura ◽  
Masashi Hosokawa ◽  
Ai Iwashima-Suzuki ◽  
Makoto Shiota ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Silicic Acid ◽  
Column Chromatography ◽  
Silicic Acid Column ◽  
Silicic Acid Column Chromatography ◽  
Wild Type ◽  
Solvent Fractionation

Purified milk sphingomyelin (SM) was obtained from lipid concentrated butter serum (LC-BS) by successive separations involving solvent fractionation, selective saponification, and silicic acid column chromatography.

Silicic acid column chromatography of phosphonolipids

1984 ◽  
Vol 294 ◽  
pp. 403-406 ◽  
Author(s):  
Michael C. Moschidis ◽  
Constantinos A. Demopoulos
Keyword(s):  
Silicic Acid ◽  
Column Chromatography ◽  
Silicic Acid Column ◽  
Silicic Acid Column Chromatography
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