Glucose uptake by diaphragms from rats subjected to hemorrhagic shock

1964 ◽  
Vol 206 (2) ◽  
pp. 317-320 ◽  
Author(s):  
William R. Drucker ◽  
John C. DeKiewiet
Keyword(s):  
Skeletal Muscle ◽  
Hemorrhagic Shock ◽  
Glucose Uptake ◽  
Anaerobic Metabolism ◽  
Lactate Production ◽  
Bicarbonate Buffer ◽  
Metabolic Alterations ◽  
Shock Models ◽  
The Media ◽  
Intracellular Energy

The marked metabolic alterations that occur in hemorrhagic shock have been ascribed to tissue anoxia occasioned by hypovolemia. Other investigators, utilizing different shock models, have explained the initial metabolic changes as secondary to humoral changes. In skeletal muscle, anoxia is known to cause an increased glucose uptake, whereas epinephrine causes a decreased uptake. The present work was undertaken to explore some alterations in carbohydrate metabolism during hemorrhagic shock in rats, when both tissue anoxia and an altered humoral state are present. Hemidiaphragms from rats subjected to a standardized hemorrhagic shock procedure and from control rats were excised and incubated aerobically in bicarbonate buffer containing glucose. After 1 hr of incubation aliquots of the media were analyzed for glucose and lactate. The results demonstrated a significantly greater glucose uptake and lactate production by the diaphragms from the bled rats. The data suggest that, during hemorrhagic shock in rats, tissue anoxia leads to a predominance of anaerobic metabolism and a severe depletion of intracellular energy, resulting in an increased uptake of glucose in skeletal muscle despite the concomitant altered humoral state which ordinarily would inhibit glucose uptake.

Increased Skeletal Muscle Na+, K+-ATPase Activity as a Cause of Increased Lactate Production after Hemorrhagic Shock

1998 ◽  
Vol 44 (5) ◽  
pp. 796-803 ◽  
Author(s):  
Fred A. Luchette ◽  
Lou Ann Friend ◽  
Carey C. Brown ◽  
Radha Krishna Upputuri ◽  
J. Howard James
Keyword(s):  
Skeletal Muscle ◽  
Atpase Activity ◽  
Hemorrhagic Shock ◽  
Lactate Production

Effects of insulin and alloxan diabetes on glucose metabolism in rabbit aortic tissue

1962 ◽  
Vol 203 (6) ◽  
pp. 1038-1042 ◽  
Author(s):  
P. D. Mulcahy ◽  
Albert I. Winegrad
Keyword(s):  
Glucose Uptake ◽  
Total Lipid ◽  
Alloxan Diabetes ◽  
Lactate Production ◽  
Aortic Tissue ◽  
Bicarbonate Buffer ◽  
Insulin Effect ◽  
Glucose Carbon ◽  
Diabetic Rabbits

Tubular sections of rabbit thoracic aorta, free of adventitia, were incubated in vitro with uniformly labeled glucose-C14 in Krebs bicarbonate buffer. Lactate production accounted for the major portion of glucose uptake, oxidation to CO2 and incorporation of glucose carbon into glycogen and total lipid, for progressively smaller fractions. Insulin in vitro had no effect on glucose uptake or lactate production by tissue from normal or alloxan diabetic rabbits. Glucose uptake, lactate production, and the incorporation of glucose carbon in CO2, glycogen, and total lipid were markedly decreased by alloxan diabetes, and were not increased in insulin in vivo until after 18 hr of therapy. Marginal but consistent increases in the incorporation of carbon 14 into total lipid occurred in tissue from normal and diabetic rabbits with insulin in vitro; similar increases in the incorporation of glucose carbon into glycogen were observed. The bulk of the cellular components of the intima and media of the rabbit aorta appear to be insensitive to a direct or immediate insulin effect, but their glucose utilization is impaired by the diabetic state.

Characteristics of rat hindlimbs perfused with erythrocyte- and albumin-free medium

1986 ◽  
Vol 251 (1) ◽  
pp. C78-C84 ◽  
Author(s):  
M. Shiota ◽  
T. Sugano
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Flow Rate ◽  
Adenine Nucleotides ◽  
Creatine Phosphate ◽  
Integral Function ◽  
Maximal Effect ◽  
Free Medium ◽  
Bicarbonate Buffer ◽  
Lactate Release

The isolated rat hindlimb was perfused with Krebs-bicarbonate buffer without erythrocytes and albumin in a flow-through mode at 32 degrees C, and the viability and metabolic characteristics of perfused skeletal muscle were examined. 1) With the flow rate at 15 ml X min-1 X leg-1, glucose and O2 uptake, lactate release, lactate-to-pyruvate ratio in effluent, and tissue creatine phosphate and adenine nucleotides remained constant at rest during perfusion for 90 min. The twitch tension changed little over perfusion. 2) When the leg was stimulated at a frequency below 0.5 Hz, the standard flow rate adequately delivered O2 to the perfused leg. Sciatic nerve stimulation enhanced glucose uptake in the absence of insulin. 3) The stimulatory effect of insulin on glucose uptake was observed with a concentration as low as 0.1 mU/ml, and maximal effect was at approximately mU/ml, with a nearly eightfold increase in glucose uptake. 4) Epinephrine and isoproterenol at a concentration of 0.5 nM stimulated lactate release, with maximal effect at 5 nM. The response to catecholamines was reversible and reproducible with a single preparation during the perfusion period of 120 min. The results indicated that the perfusion of hindlimb with a hemoglobin- and albumin-free medium is a convenient and reliable tool for the biochemical investigations of the integral function of hindlimb skeletal muscle.

scholarly journals Inhibition of glucose uptake and glycogenolysis by availability of oleate in well-oxygenated perfused skeletal muscle

1977 ◽  
Vol 168 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Michael J. Rennie ◽  
John O. Holloszy
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Glucose Uptake ◽  
Contractile Activity ◽  
Haemoglobin Concentration ◽  
Lactate Production ◽  
O2 Uptake ◽  
Perfusion Medium ◽  
Red Muscle ◽  
Intracellular Glucose

The effects of exogenous oleate on glucose uptake, lactate production and glycogen concentration in resting and contracting skeletal muscle were studied in the perfused rat hindquarter. In preliminary studies with aged erythrocytes at a haemoglobin concentration of 8g/100ml in the perfusion medium, 1.8mm-oleate had no effect on glucose uptake or lactate production. During these studies it became evident that O2 delivery was inadequate with aged erythrocytes. Perfusion with rejuvenated human erythrocytes at a haemoglobin concentration of 12g/100ml resulted in a 2-fold higher O2 uptake at rest and a 4-fold higher O2 uptake during muscle contraction than was obtained with aged erythrocytes. Rejuvenated erythrocytes were therefore used in subsequent experiments. Glucose uptake and lactate production by the well-oxygenated hindquarter were inhibited by one-third, both at rest and during muscle contraction, when 1.8mm-oleate was added to the perfusion medium. Addition of oleate also significantly protected against glycogen depletion in the fast-twitch red and slow-twitch red types of muscle, but not in white muscle, during sciatic-nerve stimulation. In the absence of added oleate, glucose was confined to the extracellular space in resting muscle. Addition of oleate resulted in intracellular glucose accumulation in red muscle. Contractile activity resulted in accumulation of intracellular glucose in all three muscle types, and this effect was significantly augmented in the red types of muscle by perfusion with oleate. The concentrations of citrate and glucose 6-phosphate were also increased in red muscle perfused with oleate. We conclude that, as in the heart, availability of fatty acids has an inhibitory effect on glucose uptake and glycogen utilization in well-oxygenated red skeletal muscle.

Metabolic alterations induced in cultured skeletal muscle by stretch-relaxation activity

1989 ◽  
Vol 256 (1) ◽  
pp. C175-C181 ◽  
Author(s):  
S. Hatfaludy ◽  
J. Shansky ◽  
H. H. Vandenburgh
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Metabolic Response ◽  
Muscle Cells ◽  
Mechanical Activity ◽  
Biphasic Response ◽  
Short Term ◽  
Metabolic Alterations

Avian pectoralis muscle cells differentiated in vitro are mechanically stimulated by repetitive stretch-relaxations of the cell's substratum. Their metabolic response to mechanical activity is determined by measuring rates of [3H]deoxy-D-glucose uptake and lactate efflux. These two metabolic parameters show a similar biphasic response to mechanical stimulation. During the first 4-6 h of activity, [3H]deoxy-D-glucose uptake and lactate efflux increase 34 and 26%, respectively; at 8 and 24 h of activity, [3H]deoxy-D-glucose uptake and lactate efflux are no longer elevated relative to control values. With continued activity beyond 24 h, their rates are again significantly elevated (150 and 93% by 48 h, respectively). The long-term increases in [3H]deoxy-D-glucose uptake and lactate efflux occur independently of medium growth factors. Protein synthesis is required for the short-term increase in [3H]deoxy-D-glucose uptake, but muscle electrical activity is not required for either short- or long-term increases in [3H]deoxy-D-glucose uptake because both occur in the presence of tetrodotoxin. This new model system allows for the analysis of mechanically induced metabolic alterations in aneural skeletal muscle cells under the defined conditions of tissue culture.

Sign in / Sign up

Export Citation Format

Share Document